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Search Results: 1 - 4 of 4 matches for " Kunjukrishnan Kamalakshi Sivakala "
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Formulation and Statistical Optimization of Culture Medium for Improved Production of Antimicrobial Compound by Streptomyces sp. JAJ06
Polpass Arul Jose,Kunjukrishnan Kamalakshi Sivakala,Solomon Robinson David Jebakumar
International Journal of Microbiology , 2013, DOI: 10.1155/2013/526260
Abstract: Streptomyces sp. JAJ06 is a seawater-dependent antibiotic producer, previously isolated and characterised from an Indian coastal solar saltern. This paper reports replacement of seawater with a defined salt formulation in production medium and subsequent statistical media optimization to ensure consistent as well as improved antibiotic production by Streptomyces sp. JAJ06. This strain was observed to be proficient to produce antibiotic compound with incorporation of chemically defined sodium-chloride-based salt formulation instead of seawater into the production medium. Plackett-Burman design experiment was applied, and three media constituents, starch, KBr, and CaCO3, were recognised to have significant effect on the antibiotic production of Streptomyces JAJ06 at their individual levels. Subsequently, Response surface methodology with Box-Behnken design was employed to optimize these influencing medium constituents for the improved antibiotic production of Streptomyces sp. JAJ06. A total of 17 experiments were conducted towards the construction of a quadratic model and a second-order polynomial equation. Optimum levels of medium constituents were obtained by analysis of the model and numerical optimization method. When the strain JAJ06 was cultivated in the optimized medium, the antibiotic activity was increased to 173.3?U/mL, 26.8% increase as compared to the original (136.7?U/mL). This study found a useful way to cultivate Streptomyces sp. JAJ06 for enhanced production of antibiotic compound. 1. Introduction Streptomyces are the prime producers of bioactive compounds for biotechnology industry. Several clinically significant antibiotics as well as widely used drugs against common diseases have been derived from this unique genus affiliated with the order Actinomycetales [1]. Diverse members of this order including Streptomyces have been isolated from previously unexplored natural habitats to nourish the current microbial antibiotic searching programs [2, 3]. Streptomyces sp. JAJ06 is a Gram-positive, moderately halophilic Streptomyces strain, which has previously been isolated from hypersaline coastal solar saltern at Tuticorin, India [4]. This strain produces an antimicrobial polyketide compound which has been reported to have potent antimicrobial activity against a set of bacteria and yeast with significant minimal inhibitory concentrations [5]. Production of antibiotic compound in JAJ06 has been reported to have seawater dependence. Like many other complex medium components, seawater has nondefined composition which might affect the
Golden Research Thoughts , 2013, DOI: 10.9780/22315063
Abstract: Indira Awas Yojana is an ambitious programme for providing shelter to the rural poor in India. The financial assistance to the IAY is shared by both the Central and State Governments. It is one of the significant policy to facilitate shelter for the rural poor. It is observed from thestatistics of Karnataka that more budget is spent on the construction of the houses compared to allocation and even more number of houses are constructed. Even though, there is much progress in the IAY, still there is demand for the houses. It shows that there is challenge from the increasing population and there are also problems such as the rich people are getting sanction of these houses.
Marcus Gunn Jaw Winking Phenomenon - A case of the widening eye
Kamalakshi G. Bhat,Anupama Karanth
Online Journal of Health & Allied Sciences , 2009,
Abstract: Marcus Gunn jaw winking phenomenon is a congenital synkinetic movement due to synkinesis between the upper eyelid and the pterygoids and it accounts for 8% of patients with congenital ptosis. In rare instances, ptosis may be absent. We present a case of Marcus Gunn Jaw Winking phenomenon without ptosis at presentation
Efficient Power Management using Adaptive Receiver Centric Transport Layer Protocols on Wireless Heterogeneous Networks
Kamalakshi.N,Dr. Naganna H,Dr Ganesh Rao
International Journal on Computer Science and Engineering , 2009,
Abstract: Today's wireless networks are highly heterogeneous, with mobile devices consisting of multiple wireless network interfaces (WNICs). Since battery lifetime is limited, power management of the interfaces has become essential with flexible and open architecture, capable of supporting various types of networks, terminals and applications. However how to integrate the protocols to meet the heterogeneous network environments becomes a significant challenge in the fourth generation wireless network. Adaptive protocols are proposed to solve heterogeneity problem in future wireless networks. This paper discusses two protocols R2CP, and RCP and feasibility of RCP protocols applied to the manage power efficiently and adaptive Congestion control on heterogeneous wireless network.
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