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Search Results: 1 - 10 of 131129 matches for " Kun-I Lin "
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α-Galactosyl Phytosphingosine 2,6’-Diamide as an Inducer of Invariant Natural Killer T Cell  [PDF]
Ying-Cheng Huang, Wei-Ting Chen, Shu-Fan Tien, Ho-Lien Huang, Chun-Nan Yeh, Kun-I Lin, Chung-Shan Yu
Open Journal of Medicinal Chemistry (OJMC) , 2013, DOI: 10.4236/ojmc.2013.33008
Abstract:

Four a-galactosyl phytosphingosine 2,6’-diamide analogs were prepared from 2,6’-diamino a-galactosylphytosphingosine and the aromatic-bearing carboxylic acids. After purification with High Performance Liquid Chromatography, a flowcytometry for the four compounds for stimulation of human Va24+/Vb11+ NKT cell populations was carried out. Additional keto groups on the acyl chains of the 2,6’-diamide compound was associated with the enhanced stimulating effect.


6-Azido-Galactosyl Imidate as a Building Block for Preparation of 1-(4-Aminobutyl)-, Di-, Tri- and Tetra-Saccharides  [PDF]
Kun-I Lin, Li-Wu Chiang, Cheng-Tse Pan, Ho-Lien Huang, Yuan-Hsiao Su, Shui-Tein Chen, Ying-Cheng Huang, Chung-Shan Yu
Open Journal of Medicinal Chemistry (OJMC) , 2013, DOI: 10.4236/ojmc.2013.33010
Abstract:

6-azidogalactosyl imidate has been used as a donor to generate 1-(4-aminobutyl)-6-aminogalactose, 6-aminothiotolyl- glycosides of disaccharide, trisaccharide and tetrasaccharide that incorporates 6-azido group and 1-(4-tolyl)thio group. Trisaccharide and tetrasaccharide were obtained from lactosyl-based acceptor. The anomeric 1-(4-tolyl)thio group could be used to conjugate with sphingosine analogs to provide the alpha-Gal Sph analogs for library extension from the azido group.

Constitutive Expression of Thermobifida fusca Thermostable Acetylxylan Esterase Gene in Pichia pastoris
Chao-Hsun Yang,Kun-I Lin,Gen-Hung Chen,Yu-Fen Chen,Cheng-Yu Chen,Wei-Lin Chen,Yu-Chun Huang
International Journal of Molecular Sciences , 2010, DOI: 10.3390/ijms11125143
Abstract: A gene encoding the thermostable acetylxylan esterase (AXE) in Thermobifida fusca NTU22 was amplified by PCR, sequenced and cloned into the Pichia pastoris X-33 host strain using the vector pGAPZαA, allowing constitutive expression and secretion of the protein. Recombinant expression resulted in high levels of extracellular AXE production, as high as 526 U/mL in the Hinton flask culture broth. The purified enzyme showed a single band at about 28 kDa by SDS-polyacrylamide gel electrophoresis after being treated with endo-β- N-acetylglycosaminidase H; this agrees with the predicted size based on the nucleotide sequence. About 70% of the original activity remained after heat treatment at 60?°C for three hours. The optimal pH and temperature of the purified enzyme were 8.0 and 60?°C, respectively. The properties of the purified AXE from the P.? pastoris transformant are similar to those of the AXE from an E. coli transformant.
Synthesis and Structure-Activity Relationships of Fenbufen Amide Analogs
Kun-I Lin,Chao-Hsun Yang,Chia-Wen Huang,Jhen-Yi Jian,Yu-Chun Huang,Chung-Shan Yu
Molecules , 2010, DOI: 10.3390/molecules15128796
Abstract: The previous discoveries of butyl fenbufen amide analogs with antitumor effects were further examined. The amide analogs with 1, 3, 4 and 8 carbons chains were prepared in 70-80% yield. Fenbufen had no cytotoxic effects at concentrations ranging from 10 to 100 μM. Methyl fenbufen amide had significant cytotoxic effects at a concentration of 100 μM. As the length of the alkyl amide side chain increased, the cytotoxic effects increased, and the octyl fenbufen amide had the greatest cytotoxic effect. After treatment with 30 μM octyl fenbufen amide, nearly seventy percent of the cells lost their viability. At the concentration of 10 μM, fenbufen amide analogs did not show cytotoxicity according to the MTT assay results. The NO scavenging activities of the fenbufen amide analogs were not significantly different from those of fenbufen.
Synthesis of Amino Core Compounds of Galactosyl Phytosyl Ceramide Analogs for Developing iNKT-Cell Inducers
Yin-Cheng Huang,Li-Wu Chiang,Kai-Shiang Chang,Wen-Chin Su,Yi-Hsian Lin,Kee-Ching Jeng,Kun-I Lin,Kuo-Yen Liao,Ho-Lein Huang,Chung-Shan Yu
Molecules , 2012, DOI: 10.3390/molecules17033058
Abstract: 1-Aminophytosphingosine and 6-aminogalactosyl phytosphingosine were prepared in 61% and 40% yield libraries with 44 carboxylic acids showed that a 4-butylbenzoic acid-derived product exe, respectively. Glycosylation using benzoyl-protected lipid resulted in better a-selectivity for ceramide analogs, but the yield was less than that obtained with benzyl moieties. Screening the amide rted less cytotoxicity. These analogs were purified for validation of immunological potencies and the a-GalCer analog but not the sphingosine analog stimulated human iNKT cell population.
Phase Identification Using Series of Selected Area Diffraction Patterns and Energy Dispersive Spectrometry within TEM  [PDF]
Kun-Lin Lin
Microscopy Research (MR) , 2014, DOI: 10.4236/mr.2014.24008
Abstract: Transmission electron microscopy (TEM) is a very powerful technique for materials characteriza-tion, providing information relating to morphology, composition, and crystal structure. Selected area diffraction patterns (SADPs) are crystallographic data that can be obtained using a TEM in-strument. Conventional identification through SADP/TEM is tricky and tedious, thereby increasing the difficulty of phase identification. To establish a procedure for phase identification of known and unknown phases, in this study we examined two samples: one, a known phase, was Si with <100> alignment; the other, unknown, was the TixOy phase at the 96.4Au-3Ni-0.6Ti interlayer/ yttria-stabilized zirconia (YSZ) interface of a steel/96.4Au-3Ni-0.6Ti interlayer/YSZ joint. The procedures for phase identification of the known and unknown phases are described herein using a series of SADPs and energy dispersive spectrometry within TEM that would be useful for general researchers.
Curcumin Reduces Amyloid Fibrillation of Prion Protein and Decreases Reactive Oxidative Stress
Chi-Fen Lin,Kun-Hua Yu,Cheng-Ping Jheng,Raymond Chung,Cheng-I Lee
Pathogens , 2013, DOI: 10.3390/pathogens2030506
Abstract: Misfolding and aggregation into amyloids of the prion protein (PrP) is responsible for the development of fatal transmissible neurodegenerative diseases. Various studies on curcumin demonstrate promise for the prevention of Alzheimer’s disease and inhibition of PrP res accumulation. To evaluate the effect of curcumin on amyloid fibrillation of prion protein, we first investigated the effect of curcumin on mouse prion protein (mPrP) in a cell-free system. Curcumin reduced the prion fibril formation significantly. Furthermore, we monitored the change in apoptosis and reactive oxygen species (ROS) level upon curcumin treatment in mouse neuroblastoma cells (N2a). Curcumin effectively rescues the cells from apoptosis and decreases the ROS level caused by subsequent co-incubation with prion amyloid fibrils. The assays in cell-free mPrP and in N2a cells of this work verified the promising effect of curcumin on the prevention of transmissible neurodegenerative diseases.
Diversity – An International and Interdisciplinary Journal
Shu-Kun Lin
Diversity , 2009, DOI: 10.3390/d1010005
Abstract: After many years of careful planning, we are pleased to launch Diversity (ISSN 1424-2818), a new international and interdisciplinary Open Access journal. Among diversity topics, biodiversity has always been a key topic. In 1996, when I was establishing Molecular Diversity Preservation International (MDPI), an organization dedicated to the collection and distribution of rare molecular samples, I read several books on biodiversity. To promote the MDPI project, in 1996 the journal Molecules was launched, where authors are encouraged to deposit authentic samples of chemicals reported in the published articles. One of the topics covered by the journal Molecules was molecular diversity, and my own paper on diversity assessment published in volume 1 of Molecules cited some of the biodiversity books I had read [1] and Molecules still has a section called “Molecular Diversity” [2]. Our founding Editor-in-Chief, Prof. Dr. Michael Wink [3], also cites biological diversity as one of his main research interests. [...]
Publisher’s Note: The Correct ISSN 2227-7102 for Education Sciences
Shu-Kun Lin
Education Sciences , 2012, DOI: 10.3390/educsci2040254
Abstract: As the registered title of our journal was changed from “Education (Basel)” (ISSN 2076-3344) to “Education Sciences” (ISSN 2227-7102) in May 2012, we mistakenly continued publishing under the old ISSN number instead of the new one. We would like to clarify that the ISSN number on the first pages of the following published papers is incorrect. The correct ISSN number should be 2227-7102. [...]
Distinguishability, Information and Useful Energies
Shu-Kun Lin
Energies , 2008, DOI: 10.3390/en1010001
Abstract: n/a
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