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Traffic Conflict Techniques in the Czech Republic
Josef Kocourek
Transactions on Transport Sciences , 2010, DOI: 10.2478/V10158-010-0017-X
Abstract: This article deals mainly with defining a way of monitoring traffic conflicts. This issue is only one part of a greater safety analysis, or risk determination process for a particular location. Based on the described procedures it is possible to apply the methodology also to more complicated nodes, such as level crossings, sections of towns, residential areas, or rural areas. After the survey and the collection of a necessary amount of transport and engineering data, the determination of a relative number for the "almost-accident rate" (number of calculated vehicles for a selected time period and the number of traffic conflicts - so-called almost-accidents) may be calculated.
Silver-Doped Layers of Implants Prepared by Pulsed Laser Deposition  [PDF]
Tomas Kocourek, Miroslav Jelinek, Jan Miksovsky, Karel Jurek, Jaromir Kopecek
Journal of Computer and Communications (JCC) , 2013, DOI: 10.4236/jcc.2013.17014
Abstract: Physical and mechanical properties of silver-doped layers of titanium alloy Ti6Al4V and 316L steel prepared by pulsed laser deposition were studied. Metallic silver-doped coatings could be a new way for antibacterial protection in medicine. Thin films of silver and silver-doped materials were synthesized using KrF excimer laser deposition. The material was ablated from two targets, which composed either from titanium alloy with silver segments or from steel with silver segments. The concentration of silver ranged from 1.54 at.% to 4.32 at.% for steel and from 3.04 at.% to 13.05 at.% for titanium alloy. The layers properties such as silver content, structure, and adhesion were measured. Adhesion was stu- died using scratch test.
O Conselho Municipal de Direitos da Crian a e do Adolescente como espa o de constru o de cidadania
Arruda, Marina Patrício de,Kocourek, Sheila
Textos & Contextos (Porto Alegre) , 2008,
Abstract: Este artigo visa contribuir na reflex o sobre os Conselhos Municipais de Direitos da Crian a e Adolescentes como espa o propício à constru o da cidadania. Nosso objetivo é mostrar que os Conselhos de Direitos sofrem de um esvaziamento e de uma retra o que diz respeito à participa o popular efetiva. O texto tem por base uma pesquisa desenvolvida junto ao Programa de Pós-Gradua o em Servi o Social, da PUCRS, e possibilitou-nos a compreens o de que a prática dos conselhos municipais reflete, de um modo geral, a trajetória brasileira de constitui o da cidadania respaldada por práticas associadas à escravid o e à passividade da popula o. Numa síntese provisória, compreendemos que a constru o da cidadania pode ser possibilitada pelos conselheiros que se disponham a repensar sua prática na condu o das reuni es e delibera es dos Conselhos Municipais, permitindo a participa o ativa da popula o.
Thermoelectric Properties of Ce0.09Fe0.67Co3.33Sb12/FeSb2Te Multi-Layered Structures  [PDF]
R. Zeipl, M. Jelínek, J. Walachová, T. Kocourek, M. Vl?ek
Journal of Computer and Communications (JCC) , 2013, DOI: 10.4236/jcc.2013.17001

Thermoelectric properties of Ce0.09Fe0.67Co3.33Sb12/FeSb2.1Te multi-layered structures with period of 5 nm were studied in temperature ranging from 300 K to 500 K. Structures were prepared by Pulsed Laser Deposition (PLD) on fused sili- ca quartz glass substrates at the substrate temperature during the deposition Ts = 230°C and Ts = 250°C with the laser beam energy density Ds = 3 Jcm-2. In the contribution temperature dependencies of the in-plane electrical conductivity, the Seebeck coefficient and the resultant power factor together with room temperature value of thermoelectric figure of merit are presented.

Thermoelectric Simple and Multilayers Prepared by Laser  [PDF]
M. Jelinek, R. Zeipl, J. Vanis, T. Kocourek, J. Remsa, J. Navratil, J. Lorin?ík
Journal of Materials Science and Chemical Engineering (MSCE) , 2016, DOI: 10.4236/msce.2016.41010

Thermoelectric layers as Bi2Te3, Yb0.19Co4Sb12, FeSb2Te, Ce0.1Fe0.7Co3.3Sb12 and FeSb2Te/Ce0.1Fe0.7Co3.3Sb12 multilayers were prepared by Pulsed Laser Deposition (PLD). Smooth, nano-crystalline, stoichiometric layers were synthetized in a classical PLD arrangement or in a special off-axis PLD arrangement, followed by Rapid thermal annealing. Results of physical characterizations such as morphology—Atomic Force Microscope, Scanning Electron Microscope, composition-Energy Dispersive X-ray analysis, crystallinity—X-ray Diffraction, separation of multilayers—Secondary of ion beam mass spectroscopy SIMS and study of thermoelectric properties such as the thermoelectric figure of merit ZT, in-plane electrical resistivity, Seebeck coefficient and thermal conductivity are presented. For thermal conductivity measurement a newly developed Atomic force thermal microscope (AFMTh) was tested. Results obtained on the single layers compared to multi-layered structures are discussed.

Hybrid Laser Technology for Creation of Doped Biomedical Layers  [PDF]
Miroslav Jelinek, Lucie Bacakova, Jan Remsa, Tomas Kocourek, Jan Miksovsky, Petr Pisarik, Marta Vandrovcova, Elena Filova, Sarka Kubinova
Journal of Materials Science and Chemical Engineering (MSCE) , 2016, DOI: 10.4236/msce.2016.41014

Hybrid laser technologies for deposition of thin films and basic schemes of combination of pulsed laser deposition (PLD) with magnetron sputtering and RF discharges or two lasers or three laser deposition systems are presented. Experiences with deposition of chromium doped diamond-like carbon (DLC) films for coating of prostheses are described. Layers of different chromium concentrations were prepared using hybrid systems (PLD + magnetron sputtering or by double PLD). Results of physical and mechanical characterization of film properties and biomedical tests of trivalent and toxic hexavalent chromium are given. Experiences with double laser deposition of DLC layers doped with silver are also mentioned.

Highly efficient concentration of lenti- and retroviral vector preparations by membrane adsorbers and ultrafiltration
Katrin Zimmermann, Oliver Scheibe, Andreas Kocourek, Jutta Muelich, Elke Jurkiewicz, Alexander Pfeifer
BMC Biotechnology , 2011, DOI: 10.1186/1472-6750-11-55
Abstract: An important feature of vectors derived from lentiviruses and prototypic gamma-retroviruses is that the host range can be altered by pseudotypisation. The most commonly used envelope protein for pseudotyping is the glycoprotein of the Vesicular Stomatitis Virus (VSV.G), which is also essential for successful concentration using UC.Here, we describe a purification method that is based on membrane adsorbers (MAs). Viral particles are efficiently retained by the anionic exchange MAs and can be eluted with a high-salt buffer. Buffer exchange and concentration is then performed by utilizing ultrafiltration (UF) units of distinct molecular weight cut off (MWCO). With this combined approach similar biological titers as UC can be achieved (2 to 5 × 109 infectious particles (IP)/ml). Lentiviral particles from small starting volumes (e.g. 40 ml) as well as large volumes (up to 1,000 ml) cell culture supernatant (SN) can be purified. Apart from LVs, vectors derived from oncoretroviruses can be efficiently concentrated as well. Importantly, the use of the system is not confined to VSV.G pseudotyped lenti- and retroviral particles and other pseudotypes can also be purified.Taken together the method presented here offers an efficient alternative for the concentration of lenti- as well as retroviral vectors with different pseudotypes that needs no expensive equipment, is easy to handle and can be used to purify large quantities of viral vectors within a short time.Lentiviral vectors (LVs) are versatile tools for molecular medicine and gene therapy [1]. They are able to integrate their viral genome into both dividing and non-dividing cells [2,3]. Most LVs presently used are based on the human immunodeficiency virus type 1 (HIV-1), which is the most extensively studied lentivirus [1]. In addition, lentivectors have been derived from a variety of other lentiviruses (e.g. simian, equine and feline lentiviruses) [4].Lenti- as well as spuma- and oncoretroviruses belong to the large fami
Ludmila K?í?OVá,Michal RICHTER,Franti?ek KOCOUREK,Jan NEDěLNíK
Journal of Central European Agriculture , 2011,
Abstract: The objective of the study was to compare the nutritive value and mycotoxin content of maize forage and silage of near isogenic control MONUMENTAL (C) and Bt maize (MONSANTO, MON 810) that was either untreated (Bt) or artificially inoculated with Fusarium strains (I-Bt). The inoculation was made in the growing crop in milk stage of maturity. Plants were harvested at the soft dough stage of maturity and ensiled in microsilage tubes. The content of forage dry matter (DM) was 307.6 g/kg in C, 306.9 g/kg in Bt and 298.0 g/kg in I-Bt. All forages were positive for deoxynivalenol, aflatoxin, fumonisins and zearalenone (P>0.05). Content of DM was the lowest in I-Bt silage (285.5 g/kg) and differed significantly from C (296.7 g/kg) or Bt (303.7 g/kg, P<0.05). Content of crude protein (CP) was the lowest in I-Bt silage (79.0 g/kg) and differed significantly from C or Bt (85.7 or 81.9 g/kg, respectively, P<0.05). Silages Bt and I-Bt had lower pH (3.93 and 3.96, respectively) than silage C (4.02, P<0.05). Silage I-Bt tended to have a higher degree of proteolysis 9.18 %) measured as N-NH3 (% of total N) than silages C or Bt (8.64 or 8.9 %, respectively, P>0.05). Lactic acid was predominant product of fermentation in all silages, however silage I-Bt tended to have lower content of lactic acid (20.96 g/kg) than C or Bt (24.76 or 23.82 g/kg, P>0.05). I-Bt silage contained lower levels of eoxynivalenol (602 ppb) than C or Bt silage (748 and 690 ppb, respectively, P<0.05). Content of fumonisins and zearalenone in C did not differ from I-Bt (P<0.05) but both were lower than in Bt (P<0.05). In conclusion, nutritional value a fermentation parameters of Bt silage were similar to C except of CP content and pH that was lower in Bt (P<0.05). I-Bt silage had lower content of DM, CP and fat than Bt silage (P<0.05). Controversially, concentrations of mycotoxins in I-Bt silage were lower than in Bt.
Rapid prefractionation of complex protein lysates with centrifugal membrane adsorber units improves the resolving power of 2D-PAGE-based proteome analysis
Mary Doud, Michael W Schmidt, David Hines, Claudia Naumann, Andreas Kocourek, Noushin Kashani-Poor, Robert Zeidler, Dieter A Wolf
BMC Genomics , 2004, DOI: 10.1186/1471-2164-5-25
Abstract: Here, we have used Vivapure Ion Exchange centrifugal adsorber units to rapidly prefractionate total fission yeast protein lysate based on protein charge. Three fractions were prepared by stepwise elution with increasing sodium chloride concentrations. Each of the fractions, as well as the total lysate, were analyzed by 2D-PAGE. This simple prefractionation procedure considerably increased the resolving power of 2D-PAGE. Whereas 308 spots could be detected by analysing total protein lysate, 910 spots were observed upon prefractionation. Thorough gel image analysis demonstrated that prefractionation visualizes an additional set of 458 unique fission yeast proteins not detected in whole cell lysate.Prefractionation with Vivapure Q spin columns proved to be a simple, fast, reproducible, and cost-effective means of increasing the resolving power of 2D-PAGE using standard laboratory equipment.Despite some limitations, 2-dimensional gel electrophoresis (2D-PAGE) [1] coupled to mass spectrometric protein identification remains one of the most reliable and reproducible means of proteome analysis. Due to the wide dynamic range of individual proteins in cells, which presumably varies over five to six orders of magnitude [2], a key requirement for a comprehensive proteome analysis is to reduce sample complexity to a level that permits access to low abundance proteins. Whereas the resolving power of 2D-PAGE is remarkable, biochemical prefractionation will further enhance resolution enabling a deeper view into complex proteomes (reviewed in [3]).Prefractionation can be achieved by a number of techniques such as differential protein extraction, purification of cell organelles or protein complexes, preparative isoelectric focusing (IEF), or chromatographic techniques [4]. Many of these procedures are time consuming, difficult to reproduce and scale up, result in sample loss, and often require expensive instrumentation such as liquid chromatography systems.Here, we have tested Vivap
Thin-Layer Hydroxyapatite Deposition on a Nanofiber Surface Stimulates Mesenchymal Stem Cell Proliferation and Their Differentiation into Osteoblasts
Eva Prosecká,Matej Buzgo,Michala Rampichová,Tomá Kocourek,Petra Kochová,Lucie Vyslou ilová,Daniel Tvrdík,Miroslav Jelínek,David Luká ,Ev en Amler
Journal of Biomedicine and Biotechnology , 2012, DOI: 10.1155/2012/428503
Abstract: Pulsed laser deposition was proved as a suitable method for hydroxyapatite (HA) coating of coaxial poly-ɛ-caprolactone/polyvinylalcohol (PCL/PVA) nanofibers. The fibrous morphology of PCL/PVA nanofibers was preserved, if the nanofiber scaffold was coated with thin layers of HA (200 nm and 400 nm). Increasing thickness of HA, however, resulted in a gradual loss of fibrous character. In addition, biomechanical properties were improved after HA deposition on PCL/PVA nanofibers as the value of Young's moduli of elasticity significantly increased. Clearly, thin-layer hydroxyapatite deposition on a nanofiber surface stimulated mesenchymal stem cell viability and their differentiation into osteoblasts. The optimal depth of HA was 800 nm.
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