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Search Results: 1 - 10 of 1310 matches for " Kazuo Onuma "
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In Situ Atomic Force Microscopy Observation of Octacalcium Phosphate (100) Face Dissolution in Weak Acidic Solutions  [PDF]
Kazuo Onuma, Mayumi Iijima
Journal of Crystallization Process and Technology (JCPT) , 2015, DOI: 10.4236/jcpt.2015.51001
Abstract: Dissolution of the (100) face of octacalcium phosphate (OCP) single crystal in weak acidic solutions (pH = 6.5; 25°;C) was observed in situ using atomic force microscopy. Monomolecular steps (2.0 nm high) were observed; they originated from etch pits or crystal edges. Advancement of the dissolution process led to precipitation of nanoparticles as small as ~10 nm even though the solution was undersaturated with respect to OCP. This precipitation of nanoparticles was accompanied by a drastic decrease in the dissolution rate; however, the substrate OCP continued to dissolve, indicating that dissolution and growth occurred simultaneously on the same surface. The precipitated nanoparticles coalesced and eventually covered the entire surface without changing the surface morphology of the substrate crystal. The step height after complete coverage was ~2.0 nm, the same as that observed on the dissolving OCP surface. These findings indicate that the precipitated phase was a pseudomorph of OCP crystal.
Crystallization of Cellobiohydrolase in the Presence of Cellulose-Degraded Cellobiose: Analysis of Intermolecular Interactions and Association Dynamics  [PDF]
Kazuo Onuma, Naoki Furubayashi, Fujiko Shibata, Yoshiko Kobayashi, Sachiko Kaito, Yuki Ohnishi, Koji Inaka
Journal of Crystallization Process and Technology (JCPT) , 2014, DOI: 10.4236/jcpt.2014.41001
Abstract:

Crystallization of enzymes in presence of impurities is important for clarifying the role of enzymes in natural world. Although it is proposed that impurities inhibit nucleation of enzyme crystallization, details are unclear. In this study, crystallization of cellobiohydrolase from Aspergillus niger was investigated by dynamic and time-resolved static light scattering using cellobiose as an impurity. We aimed to clarify how cellobiose inhibits cellobiohydrolase crystallization and to crystallize cellobiohydrolase in concentrated cellobiose without using seeds. The contribution of attractive forces to total intermolecular interactions of cellobiohydrolase monomers increased with the molar ratio of cellobiose/cellobiohydrolase (R(cb/ce)). Association dynamics of cellobiohydrolase using lithium sulfate, however, showed that the initial aggregation rate decreased with an increase in R(cb/ce). Because binding sites of cellobioses to cellobiohydrolase molecules differed from those for the growth of protein crystals, the binding of cellobioses would increase the chemical potential of the cellobiohydrolase monomers, which gradually reduced supersaturation for growth as the aggregate size increased. This result was in contrast with the conventional idea that cellobiose inhibits the nucleation of cellobiohydrolase crystals. Gentle agitation of cellobiose-containing cellobiohydrolase solutions during sitting-drop vapor-diffusion growth resulted in the growth of cellobiohydrolase single crystals for all R(cb/ce) conditions without using seeds.

Non-motor signs and symptoms in Parkinson’s disease  [PDF]
Kazuo Abe
Health (Health) , 2012, DOI: 10.4236/health.2012.431171
Abstract: Motor symptoms are cardinal clinical features of Parkinson’s disease (PD). Progress in drug therapy and rehabilitation has been presenting beneficial effect for motor symptoms. However, non-motor symptoms and signs in PD have been accumulated growing attentions and its amelioration may also give beneficial effect for PD patients’ and their care givers’ quality of life. In this mini-review, I overviewed non-motor symptoms and signs in PD.
A Revival of Little Black Sambo in Japan  [PDF]
Kazuo Mori
Advances in Literary Study (ALS) , 2014, DOI: 10.4236/als.2014.21003
Abstract:

In the late 1980s, a popular children’s book “Little Black Sambo”(hereafter,LBS) disappeared from all bookstores in Japan.The book was alleged to have racist characteristics such as the name of the boy, the way the illustrations caricatured blacks, etc. Mori (1997) revised the original story by changing the protagonist from a black boy to a black Labrador puppy, with eliminating the word “Sambo”,which had a historically pejorative connotation in the US. Mori (2005) conducted an experiment to compare the entertainment value of the two versions of LBS using four-year-old children and found no difference. Mori (2005) also casted a suspicion that the real reason why the book was withdrawnin Japan was a matter of piracy rather than racism. All Japanese publishers at that time had not properly obtained the copyright. Nowadays there are several versions of LBS available in bookstores all over Japan.

Getting Rid of the Horizontal-to-Vertical Strategy: A Case Report of a Japanese Research Psychologist  [PDF]
Kazuo Mori
Open Journal of Social Sciences (JSS) , 2014, DOI: 10.4236/jss.2014.24040
Abstract: Most Japanese research psychologists have used “Horizontal-to-Vertical Conversion Strategy” for their research, simply replicating experimental studies published in Western journals with Japanese participants and publishing papers only in Japanese (the traditional orthography of the Japanese language is vertical). This short article criticized this prevailing research strategy among Japanese psychologists and reported a series of studies resulted from an invention of new experimental procedure. It showed an example of a Japanese researcher who had got rid of the inadequate approach by inventing a new experimental method. It also aimed to encourage Japanese psychologists to conduct innovative research and publish their studies in English journals.
Assessment of the Implicit Attitude of Japanese People toward Blacks and Little Black Sambo  [PDF]
Kazuo Mori
Open Journal of Social Sciences (JSS) , 2018, DOI: 10.4236/jss.2018.61001
Abstract:
We examined whether Japanese people, 47 junior high school students, 49 undergraduates, and 52 older adults, possessed negative attitudes against blacks and the picture book Little Black Sambo. We assessed the implicit attitude toward the target word pairs, “black/white” and “Sambo/Heidi,” by utilizing a paper-based Implicit Association Test and found that both black and Sambo were associated more negatively than white and Heidi. However, the implicit attitudes assessed with a single-target IAT showed that 67 Japanese students showed positive implicit scores for blacks but with smaller valences. A post hoc analysis revealed that the reading experience of Little Black Sambo did not show a significant difference between the implicit attitudes of those who had and had not read the book.
Automatic & Safe Oxytocin Induction of Labor  [PDF]
Kazuo Maeda
Open Journal of Obstetrics and Gynecology (OJOG) , 2018, DOI: 10.4236/ojog.2018.89084
Abstract: Aims: To prevent fetal damage by excess oxytocin administration of manually controlled infusion, by automatic and safe increasing infusion setting with monitoring uterine contraction and fetal heart rate. Methods: Starting infusion level was 2 milliU/ml, as oxytocin sensitivity of a pregnant uterus is unknown, to avoid hyper contraction and fetal bradycardia caused by unexpected excess oxytocin sensitivity. The infusion automatically increased with automatic monitoring of uterine contraction curve, then the increasing stopped when contraction reached to normal labor level, where the infusion level maintained, which continued until delivery, if there is no trouble. However, the infusion continued until expiring all fluid in case of insensitive uterus, where the induction was performed in another day. The infusion stopped automatically when contraction was too strong, or fetal heart rate is abnormal. Thus, oxytocin sensitive case is protected from excess contraction and fetal asphyxia. Results: Normal vaginal delivery was achieved in 28/33 cases (85%), which was more than manually controlled infusion. No case was abnormal in successful oxytocin infusion. Conclusion: The automated technique will be applied to oxytocin labor induction.
Usefulness of Radiofrequency Ablation with the PVT-350BTP Probe for the treatment of Hepatocellular Carcinoma  [PDF]
Naoki Hotta, Kazuo Masuko
Open Journal of Medical Imaging (OJMI) , 2011, DOI: 10.4236/ojmi.2011.11002
Abstract: Radiofrequency ablation (RFA) has been clinically employed in Japan, and its usefulness in the treatment of hepatocellular carcinoma (HCC) has been reported by many researchers. In the present study, the convex puncture probe PVT-350BTP, rather than a conventional convex probe, was used for RFA for the treatment of HCC and its usefulness was assessed. At the same time, evaluation of the therapeutic effect using ultrasound contrast agent was investigated. The subjects were 20 patients (16 men and 4 women) with 20 HCC nodules as confirmed by contrast CT or abdominal angiography. No nodules were ≤1.0 cm in diameter, 12 were 1.1 cm - 2.0 cm, and 8 were 2.1 cm - 3.0 cm. An Aplio diagnostic ultrasound system (Toshiba Medical Systems, Tokyo, Japan) and the PVT-350BTP probe (Toshiba) were employed for RFA. The PVT-350BTP supports puncture angles of 55, 70, 85, and 100 degrees. By mounting various types of puncture adapters on the probe, 13 G to 22 G puncture needles can be used. The needle can easily be attached and detached by operating a lever. Contrast echo studies with Levovist were performed before and after RFA, and the results were evaluated using the Advanced Dynamic Flow (ADF) imaging technique. Puncture was performed successfully using this probe for 20 cases with 20 nodules. It was confirmed that RFA with the PVT-350BTP permits percutaneous treatment to be performed more reliably than with a conventional probe.
Conformity of Six-Year-Old Children in the Asch Experiment without Using Confederates  [PDF]
Aiko Hanayama, Kazuo Mori
Psychology (PSYCH) , 2011, DOI: 10.4236/psych.2011.27100
Abstract: We investigated the conformity of young children without the use of confederates by utilizing the fMORI-Asch paradigm. The Asch-equivalent tasks were presented by means of a presentation trick so that one participant observed different stimuli than the other three, creating a minority-majority confrontation without using confederates. Ninety-six Japanese first graders (6-7 years old; 48 boys and 48 girls) participated in same-sex groups of four. The response order was randomly assigned and the third responders observed the standard lines differently from the other three children. The results showed that the minority children who had observed different stimuli tended to make more errors than the other three children. No gender differences were observed.
Usefulness of new live 3D ultrasound probe during radiofrequency ablation in a case of hepatocellular carcinoma  [PDF]
Naoki Hotta, Kazuo Masuko
Open Journal of Gastroenterology (OJGas) , 2012, DOI: 10.4236/ojgas.2012.21003
Abstract: We report a case of hepatocellular carcinoma (HCC) with chronic hepatitis C virus (HCV) infection, suc-cessfully treated with percutaneous radiofrequency ablation (RFA) under new Live trhree-dimensional (3D) echo guidance for hearts probe before [1], but ,at the time, perform RFA with a new Live3D probe de- veloped flants. A 61 year-old Japanese man had a 3.5 cm in diameter HCC nodule in the liver S3 region. We applied the Live 3D ultrasonography during RFA therapy with a cool-tip needle electrode. The echo guidance facilitated an accurate approach for the needle puncture. The guidance was also useful for confirming, whether an adequate safety marging for the nodule had been obtained. Thus new Live 3D ul-trasonography echo technique appears to provide safe guidance of RFA needles via accurate targeting of HCC nodules, thereby allowing real-time visualize- tion when combined with echo contrast.
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