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Search Results: 1 - 10 of 26 matches for " Kantamaht KANCHANAPOOM "
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Micropropagation through adventitious shoot regeneration from leaf culture of Torenia fournieri Lind.
Kantamaht Kanchanapoom,Nirunya Buntin,Kamnoon Kanchanapoom
Songklanakarin Journal of Science and Technology , 2010,
Abstract: A tissue culture system was established from young leaves of in vitro grown seedling of Torenia fournieri Lind. Leafsegments were cultured on Murashige and Skoog medium (MS) medium supplemented with combinations of NAA and BA. Shoot organogenesis was observed in all growth regulators containing medium. High frequency regeneration was obtained from leaves cultured on MS medium supplemented with 0.05 mg/l NAA plus 3 mg/l BA. When individual isolated shoots were transferred to MS medium devoid of growth regulators, complete plantlets were obtained. Adventitious shoots were also regenerated from callus that was derived from leaf explants and a high frequency of shoot organogenesis was found on MSmedium containing 0.1 mg/l NAA plus 3 mg/l BA.
In vitro Flowering of Shoots Regenerated from Cultured Nodal Explants
Kantamaht KANCHANAPOOM,Suttinee JINGJIT,Kamnoon KANCHANAPOOM
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2011,
Abstract: A protocol for the regeneration of Gypsophila paniculata L. using nodal explants from 2-month-old field grown plants was established. The induction of multiple shoots was best obtained on Murashige and Skoog (MS) medium supplemented with 13.3 μM BA. Callus growth was observed on MS medium containing 44.3 μM BA. Calluses were transferred to MS medium supplemented with 2, 4-D (4.5, 13.5, 22.6 μM), NAA (5.3, 16.1, 26.8 μM) or BA (4.4, 13.3, 22.1 μM) for 2 months to induce shoot formation. After 6 weeks of initial culture, multiple shoots were regenerated from calluses cultured on MS medium supplemented with 13.3 μM BA. All regenerated shoots produced roots on 16.1 μM NAA containing MS medium within 4 weeks. Rooted plantlets were hardened and established in pots at 100% survival. For induction of in vitro flowering, regenerated shoots could be induced to flower efficiently when cultured on MS medium containing 13.3 μM BA and 50 g/l sucrose.
In Vitro Flowering from Cultured Nodal Explants of Rose (Rosa hybrida L.)
Kantamaht KANCHANAPOOM,Nonlapan POSAYAPISIT,Kamnoon KANCHANAPOOM
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2009,
Abstract: Roses are one of the world’s most important ornamentals for a long time and are most often used for ornamental, medicinal and aromatic purposes. The study reports in vitro multiple shoot formation and flower induction of Rosa hybrida L. cv. ‘Red Masterpiece’ with maximum number of 5 shoots per explant on MS medium supplemented with 3 mg/l BA and 1 mg/l kinetin, followed by flower induction on MS medium containing 2 mg/l BA for 9 weeks. The shoots readily rooted on MS medium devoid of growth regulators. Shoots cultured under various photoperiods did not flower. Rooted plantlets were hardened and established in pots with 100% survival.
The Effect of Chitosan on Organogenesis of Oil Palm Embryo-Derived Callus
Kantamaht KANCHANAPOOM,Amornrat PHONGDARA,Kamnoon KANCHANAPOOM
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2010,
Abstract: Zygotic embryos of oil palm (Elaeis guineensis Jacq. var. tenera) were excised and cultured on MS medium containing 3 mg/l 2, 4-D either with or without 0.05% activated charcoal (AC). Improved growth of embryos was obtained on MS medium supplemented with 0.05% AC. Callus cultures were initiated from embryos, young leaves and roots on MS medium containing 2, 4-D, NAA and 0.05% AC. On these media, two morphologically distinct types of white and yellow compact calluses were produced. Green shoots regenerated after several transfers of the yellow compact calluses from zygotic embryos to MS medium supplemented with 15 mg/l chitosan either with or without 5 mg/l 2, 4-D. Histological sectioning revealed that regenerated shoots originated from a clump of meristematic cells that had dense cytoplasm. Regenerated shoots rooted when transferred to MS medium in the presence of 0.05% AC. Transfer of plantlets to soil was achieved. Callus from young seedling leaves and roots did not regenerate shoots or roots in medium containing 2, 4-D or TDZ, with or without chitosan. This finding shows that chitosan can initiate organogenesis in oil palm callus.
Micropropagation of Anubias barteri var. Nana from Shoot Tip Culture and the Analysis of Ploidy Stability
Kantamaht KANCHANAPOOM,Panyaros CHUNUI,Kamnoon KANCHANAPOOM
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2012,
Abstract: Plant regeneration of Anubias barteri var. Nana was achieved through organogenesis in shoot tip cultures. Multiple shoots were induced from cultured shoot tips on a modified MS (Murashige and Skoog, 1962) medium supplemented with BA and kinetin. The maximum green shoot numbers were best obtained on MS medium containing 3 mg/L BA with 5 shoots. Rooting in all regenerated shoots was promoted on MS medium devoid of plant growth regulators or kinetin singly. Acclimatization and survival when transferred to field conditions were shown to be 100% in the regenerated plants. Cytological and flow cytometric analyses of the mother plants and in vitro grown plants derived from 5 years old cultures showed no differences in ploidy level, they were all diploid (2n = 2x = 48) with a 2C peak indicating that ploidy alteration did not occur.
In vitro Propagation of Adenium obesum (Forssk.) Roem. and Schult.
Kantamaht KANCHANAPOOM,Sunisa SUNHEEM,Kamnoon KANCHANAPOOM
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2010,
Abstract: An in vitro protocol using shoot tip explants from seedling of Adenium obesum (Forssk.) Roem. and Schult. was developed. Explants were cultured on Murashige and Skoog (MS) medium supplemented with various combinations of NAA and BA. The most effective medium for shoot proliferation at a high frequency of 5.20±1.10 shoots per explant consisted of 22.2 μM BA. High rooting and survival were achieved using MS medium supplemented with 0.3% activated charcoal and without any growth regulators. Rooted plants were successfully acclimatized to greenhouse conditions. This study showed that A. obesum could be micropropagated by utilizing multiple shoots derived from seedling shoot tips. A flow cytometric analysis for DNA content revealed no differences among the micropropagated plants and the in vivo natural grown plants. The resulting 2C DNA value (8.35±0.039 pg) of this species was estimated for the first time.
Micropropagation from cultured nodal explants of rose (Rosa hybrida L. cv. ‘Perfume Delight’)
Naphaporn Nak-Udom,Kantamaht Kanchanapoom,Kamnoon Kanchanapoom
Songklanakarin Journal of Science and Technology , 2010,
Abstract: A method for the micropropagation of rose (Rosa hybrida L. cv. ‘Perfume Delight’) was developed. First to fifth nodal explants from young healthy shoots were excised and cultured on basal medium of Murashige and Skoog (1962, MS) containing several concentrations of BA and NAA. Multiple shoot formation of up to 3 shoots was obtained on MS medium supplemented with 3 mg/l BA and 0.003 mg/l NAA. Shoot readily rooted on MS medium devoid of growth regulators.Rooted plantlets were hardened and established in pots at 100% survival. In vitro flowering was observed on rose plantscultured on MS medium containing 3 mg/l BA and 0.003 mg/l NAA.
Determination of the most efficient target tissue and helium pressure for biolistic transformation of oil palm (Elaeis guineensis Jacq.)
Kantamaht Kanchanapoom,Alisa Nakkaew,Kamnoon Kanchanapoom,Amornrat Phongdara
Songklanakarin Journal of Science and Technology , 2008,
Abstract: An efficient genetic transformation system for oil palm using particle bombardment was established. The transformation was performed using the pCAMBIA 1302 DNA which contains the green fluorescent protein (mgfp5) reporter gene and the selectable marker hygromycin phosphotransferase (hph) gene. Oil palm explants were bombarded under the following conditions: rupture disk to macrocarrier distance, 11 mm; macrocarrier to target tissue, 90 mm and using 1 μm gold particles as microcarrier. Four different pressures of helium were tested with three types of target tissues (mature embryo, embryogenic callus and young seedlings). From the transformation efficiency, calli were much more efficiently transformed in the biolistic process compared with mature embryos and seedlings. A 100% transformation efficiency for DNA delivery into callus oil palm explants was obtained at 850 psi helium pressures, for embryos a maximum 81.8% efficiency required 850 psi and for seedlings a maximum 75.9% efficiency required 1,550 psi. Using a confocal laser scanning microscope, and appropriate filters to block out the red fluorescence of chlorophyll, expression of the GFP gene was observed in all three bombarded explant types by a bright-green fluorescence. The mgfp5 gene was still present more than 8 months after bombardment, hence it indicated the stability of transgene in those transformants.
Efficient Biolistic Transformation and Regeneration Capacity of an EgTCTP Transgene in Protocorm-like Bodies of Phalaenopsis Orchid
Kantamaht KANCHANAPOON,Alisa NAKKAEW,Kamnoon KANCHANAPOOM,Amornrat PHONGDARA
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2012,
Abstract: An efficient genetic transformation system using the biolistic method and protocorm-like bodies (PLBs) of Phalaenopsis orchid was established for introducing the EgTCTP gene obtained from oil palm leaves. A pCAMBIA 1302 vector containing the green fluorescent protein (mgfp5) as reporter gene and the selectable marker hygromycin phosphotransferase (hpt) gene under the cauliflower mosaic virus (CaMV) 35S promoter were used in this experiment. The transformed PLBs were cultured on MS medium containing 20 mg/L hygromycin for 2 months. The surviving PLBs with green fluorescence spots were used to calculate a transformation frequency (93.34%). PLBs containing the transformed EgTCTP gene had the highest percentage of regeneration frequency (95.66%) and numbers of regenerated shoots per explant (3.78±1.89 shoots) compared to the control. The time required for initiation of primordial shoots in the transformed PLBs (55.22±26.56 days) was much shorter than for the control. Evaluation of the regeneration efficiency, determined that the status of the EgTCTP transformants was above average: score=4.04±0.88. The EgTCTP gene was detected in the PLBs over a period of at least 6 months with subculturing every 4 weeks. The stability of the transgenes within the PLBs was confirmed by PCR and this indicated that the transgenes had been integrated into the genome of the transformants. This is the first successful report to introduce EgTCTP gene into PLBs of Phalaenopsis orchid.
Histology of embryoid development in oil palm (Elaeis guineensis Jacq.) cell suspension culture
Kamnoon Kanchanapoom,Songrat Tinnongjig
Songklanakarin Journal of Science and Technology , 2001,
Abstract: Embryos of oil palm (Elaeis guineensis Jacq.) variety tenera were cultured on Eeuwens or Y3 (1976; 1978) medium supplemented with 2 mg/l 2,4-D. Calluses were initiated from these embryos. The eight-weekold calluses derived from embryos were transferred to modified Y3 liquid medium devoid of 2,4-D and supplemented with NAA, BA and coconut water to establish cell suspension culture. After a period of culture,these cells were then subcultured to the same medium without plant growth regulators to induce embryoid formation. The calluses and embryoids were harvested at various times, fixed, sectioned, stained and examined microscopically. Histological study revealed that embryoid occurred from meristematic cells with dense cytoplasm along the callus clumps.
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