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Search Results: 1 - 10 of 289 matches for " Kalpana Rajanala "
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Localization of Nucleoporin Tpr to the Nuclear Pore Complex Is Essential for Tpr Mediated Regulation of the Export of Unspliced RNA
Kalpana Rajanala, Vinay Kumar Nandicoori
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0029921
Abstract: Nucleoporin Tpr is a component of the nuclear pore complex (NPC) that localizes exclusively to intranuclear filaments. Tpr functions as a scaffolding element in the nuclear phase of the NPC and plays a role in mitotic spindle checkpoint signalling. Export of intron-containing mRNA in Mason Pfizer Monkey Virus is regulated by direct interaction of cellular proteins with the cis-acting Constitutive Transport Element (CTE). In mammalian cells, the transport of Gag/Pol-CTE reporter construct is not very efficient, suggesting a regulatory mechanism to retain this unspliced RNA. Here we report that the knockdown of Tpr in mammalian cells leads to a drastic enhancement in the levels of Gag proteins (p24) in the cytoplasm, which is rescued by siRNA resistant Tpr. Tpr's role in the retention of unspliced RNA is independent of the functions of Sam68 and Tap/Nxf1 proteins, which are reported to promote CTE dependent export. Further, we investigated the possible role for nucleoporins that are known to function in nucleocytoplasmic transport in modulating unspliced RNA export. Results show that depletion of Nup153, a nucleoporin required for NPC anchoring of Tpr, plays a role in regulating the export, while depletion of other FG repeat-containing nucleoporins did not alter the unspliced RNA export. Results suggest that Tpr and Nup153 both regulate the export of unspliced RNA and they are most likely functioning through the same pathway. Importantly, we find that localization of Tpr to the NPC is necessary for Tpr mediated regulation of unspliced RNA export. Collectively, the data indicates that perinuclear localization of Tpr at the nucleopore complex is crucial for regulating intron containing mRNA export by directly or indirectly participating in the processing and degradation of aberrant mRNA transcripts.
Characterization of Leishmania donovani MCM4: Expression Patterns and Interaction with PCNA
Neha Minocha, Devanand Kumar, Kalpana Rajanala, Swati Saha
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0023107
Abstract: Events leading to origin firing and fork elongation in eukaryotes involve several proteins which are mostly conserved across the various eukaryotic species. Nuclear DNA replication in trypanosomatids has thus far remained a largely uninvestigated area. While several eukaryotic replication protein orthologs have been annotated, many are missing, suggesting that novel replication mechanisms may apply in this group of organisms. Here, we characterize the expression of Leishmania donovani MCM4, and find that while it broadly resembles other eukaryotes, noteworthy differences exist. MCM4 is constitutively nuclear, signifying that, unlike what is seen in S.cerevisiae, varying subcellular localization of MCM4 is not a mode of replication regulation in Leishmania. Overexpression of MCM4 in Leishmania promastigotes causes progress through S phase faster than usual, implicating a role for MCM4 in the modulation of cell cycle progression. We find for the first time in eukaryotes, an interaction between any of the proteins of the MCM2-7 (MCM4) and PCNA. MCM4 colocalizes with PCNA in S phase cells, in keeping with the MCM2-7 complex being involved not only in replication initiation, but fork elongation as well. Analysis of a LdMCM4 mutant indicates that MCM4 interacts with PCNA via the PIP box motif of MCM4 - perhaps as an integral component of the MCM2-7 complex, although we have no direct evidence that MCM4 harboring a PIP box mutation can still functionally associate with the other members of the MCM2-7 complex- and the PIP box motif is important for cell survival and viability. In Leishmania, MCM4 may possibly help in recruiting PCNA to chromatin, a role assigned to MCM10 in other eukaryotes.
Geostatistical Analyst for Deciding Optimal Interpolation Strategies for Delineating Compact Zones  [PDF]
Kalpana Harishwar Kamble, Pramila Aggrawal
International Journal of Geosciences (IJG) , 2011, DOI: 10.4236/ijg.2011.24061
Abstract: Variability maps of Hydraulic conductivity (K) were generated by using geo statistical analyst extension of ARC GIS for delineating compact zones in a farm. In the initial exploratory spatial data analysis, K data for 0 - 15 and 15 - 30 cm soil layers showed spatial dependence, anisotropy, normality on log transformation and linear trend. Outliers present in both layers were also removed. In the next step, cross validation statistics of different combinations of kriging (Ordinary, simple and universal), data transformations (none and logarithmic) and trends (none and linear) were compared. Combination of no data transformation and linear trend removal was the best choice as it resulted in more accurate and unbiased prediction. It thus, confirmed that for generating prediction maps by kriging, data need not be normal. Ordinary kriging is appropriate when trend is linear. Among various available anisotropic semivariogram models, spherical model for 0 - 15 cm and tetra spherical model for 15 - 30 cm were found to be the best with major and minor ranges between 273 - 410 m and 98 - 213 m. The kriging was superior to other interpolation techniques as the slope of the best fit line of scatter plot of predicted vs. measured data points was more (0.76) in kriging than in inverse distance weighted interpolation (0.61) and global polynomial interpolation (0.56). In the generated prediction maps, areas where K was <12 cm?day–1 were delineated as compact zone. Hence, it can be concluded that geostatistical analyst is a complete package for preprocessing of data and for choosing the optimal interpolation strategies.
$\nu$ masses in a SUSY SO(10) theory with spontaneous CP violation
Bora, Kalpana
High Energy Physics - Phenomenology , 2007,
Abstract: We propose a possibility of spontaneous CP-violation (SCPV) at high scale in a SUSY SO(10) theory. The model is L-R symmetric SUSY SO(10) with \textbf{\noun{10}} and \textbf{126} dimensional Higgs generating fermion masses, and the CP phase is generated through complex VEV of B-L breaking \textbf{126} Higgs . The model can have potential application in explaining $\nu$ masses and leptogenesis as well.
Use of Information Communication Technology (ICT) in communicable disease surveillance – a review of literature
Kalpana Chandrasekar
Sri Lanka Journal of Bio-Medical Informatics , 2012, DOI: doi: http://dx.doi.org/10.4038/sljbmi.v2i2.3541
Abstract: Panic set out by newly emerging and re-emerging communicable diseases is taking a global dimension. A functional surveillance system is essential in all countries to provide the necessary information required for preventive action against communicable diseases and to make decisions on public health related issues. This article discusses the importance of such ‘communicable disease surveillance systems (CDSS)’ and problems with existing CDSS. Most of the studies conducted were related to the attributes such as completeness and timeliness of communicable disease notification. As a solution to overcome the difficulties experienced by the existing systems, some countries have introduced computerised systems for the surveillance of communicable diseases. Studies focused on the evaluation of these electronic systems reported improvements in the quality of the systems with respect to the previous systems. During the last few years, studies were directed towards the development and evaluation of ‘early warning systems’ and ‘syndromic surveillance systems’ for early detection and monitoring of epidemics and bioterrorist-initiated infectious disease outbreaks. The WHO envisages an integrated approach to communicable disease surveillance and this strategy has been recognised by all Member States and is being adapted in the African region and activities are under way in the Eastern Mediterranean, Europe and South-East Asia.
BIO-PROGRAMMING PROSPECTS OF JAVA: A COMPUTATIONAL MOVE TOWARDS THE UNDERSTANDING OF THE BIOLOGICAL ASPECTS OF GENES AND PROTEINS
KALPANA RAJA
Indian Journal of Computer Science and Engineering , 2011,
Abstract: Java is a powerful object oriented programming language that dominates many other programming languages for more than a decade. It is well designed and available as many executable technologies for software development such as Java Swing, Java Beans, Java Applets, Java Web Start, Java Database Connectivity (JDBC), Java Server Pages (JSP) and Java 2 Enterprise Edition (J2EE). Beyond its usage in the IT sector, the language is prominent even in the new emerging fields including bioinformatics and computational biology.The biological data (genes and proteins) from the biological and medical research is immense and require software professionals to mine them for new knowledge discovery. The knowledge to merge the programming concepts of Java to understand a wide range of biological concepts opens a new career challenge for many IT professionals. This paper introduces the implementation of the coding knowledge of Java in the field ofmolecular biology.
HPLC ANALYSIS OF EXTRACT OF IN VIVO MEDICINALLY IMPORTANT CLIMBER CISSUS QUADRANGULARIS L. (HADJOD)
Teware Kalpana
International Research Journal of Pharmacy , 2013,
Abstract: Cissus qudrangularis L. (Hadjod) belongs to family Vitaceae is an indigenous medicinal plant of India. It has been prescribed in ancient Ayurvedic texts as a general tonic especially for the fractured patient. The stem of Cissus quadrangularis L. is also reputed in Ayurveda as alterative, anthelmintic, dyspeptic, digestive, tonic, analgesic in eye and ear diseases, in the treatment of irregular menstruation and asthma, and in complaints of the back and spine. The plant extracts also exhibit cardio tonic property. In present study reveals the presence of various secondary metabolites as Alkaloids, Carbohydrates, Flavones and flavonoids, Saponins, Steroids and sterols, in various types of extracts. Preliminary qualitative chemical tests of extracts were found positive for Phytosterols, flavonoids and triterpenoids in Ethyl acetate fraction. Two important flavonoids were reported in the extract of this highly important medicinal plant Cissus quadrangularis L. HPLC analysis of the extract of in vivo plant give support to the presence of important flavonoids. This piece of work reveals that this climber has important pharmacological properties. The results of the study could be useful for further researches in the world of pharmacology and serve society.
MICROSPONGE: A VERSATILE VESICULAR APPROACH FOR TRANSDERMAL DRUG DELIVERY SYSTEM
Kalpana Patidar
Journal of Global Pharma Technology , 2010, DOI: 10.1234/jgpt.v2i3.11
Abstract: Transdermal drug delivery system (TDDS) is not practically for delivery of materials whose final target is skin itself. Application of topical drugs suffers many problems such as ointments, which are often aesthetically unappealing, greasiness, stickiness etc. that often results into lack of patient compliance. Microsponge technology offers entrapment of ingredients and is believed to contribute towards reduced side effects, improved stability, increased elegance, and enhanced formulation flexibility. In addition, numerous studies have confirmed that Microsponge delivery systems (MDS) are non-irritating, non-mutagenic, non-allergenic, and non-toxic. MDS technology is being used currently in cosmetics, over-the-counter (OTC) skin care, sunscreens and prescription products.
E-Governance in Rural India: Need of Broadband Connectivity Using Wireless Technology  [PDF]
Kalpana Chaudhari, Upena Dalal, Rakesh Jha
Wireless Engineering and Technology (WET) , 2011, DOI: 10.4236/wet.2011.23029
Abstract: The Information and Communication Technologies play an important role in rural development. The Empowerment of rural communities is crucial for the development of the rural region. Bringing the people in the rural region in the mainstream of the digital technologies to access and adopt modern technologies is a major concern now. Rural Development implies both, the economic development of the people and greater social transformation using electronic governance (e-governance). In order to provide the rural people in Maharashtra with better prospects and opportunities for economic development, agricultural development and management, marketing management, increased participation of rural people in usage and adoption of information and communication technologies (ICTs) is envisaged. This paper aims to explore the nature, role and relevance of the Electronic/Digital Governance using ICTs and wireless technologies for agriculture and rural development in the rural regions. It also aims to study the impact of e-governance on rural development and methods for improving local environmental governance having regard particularly to the range of interests and actors involved in e-governance. The paper examines the current status of electronic governance in Jalgaon district in India. It focuses on development of the model using ICTs/wireless technologies for e-governance of Jalgaon district in Maharashtra state, India.
Addressing the health needs of south Asians: The community perspective  [PDF]
Nitasha Chaudhary, Amita Vyas, Kalpana Ramiah
Health (Health) , 2012, DOI: 10.4236/health.2012.410121
Abstract: Community based organizations play a vital role in the health of communities in the United States, particularly among immigrant and minority populations. Yet the gap between the needs of the community and the health issues that are being addressed remains quite large. This qualitative assessment provides a brief summary of community-based organizations in the US that focus on health issues specifically addressing the needs of South Asian Americans. In addition, this assessment explores the health needs of the South Asian American community living in the Washington DC metropolitan region, the fifth largest densely populated city in the US of South Asian Americans. While the report provides an array of organizations serving this population, the question of how effective can community-based organizations be if they can not prioritize their needs based on the community’s needs still remains.
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