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Search Results: 1 - 10 of 715 matches for " KUSUMADEWI SRI YULITA "
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Secondary Structures of Chloroplast trnL Intron in Dipterocarpaceae and its Implication for the Phylogenetic Reconstruction
HAYATI Journal of Biosciences , 2013,
Abstract: Unambiguous insertion-deletion events were previously identified in trnL intron of 110 species of subfamily Dipterocarpoideae (Dipterocarpaceae). These indels are associated with the formation of four stem loop structures and featuring characteristic for generic/infra-generic level depended upon which taxonomic classifications are followed. Phylogenetic analyses were performed by including and excluding these structures to examine the robustness of resulted topologies. Results indicated that inclusion of such structures yielded more resoved topologies, and that none of the stemloop structures were homoplasious. Results of this present study was also in agreement with the previous molecular phylogenetic studies that using several genes of cp genomes in that tribe Dipterocarpae was polyphyletic by the placement of all members of the genus Dipterocarpus within tribe Shoreae, and that tribe Shoreae was a potential monophyletic group. The phylogenetic relationships between variable genera of Hopea and Shorea was also in accordance to earlier studies that suggested a potential monophyly of the two with inclusion of Parashorea and Neobalanocapus heimii. Genera that were recived strong branch support (Dipterocarpus, Dryobalanops, Vatica, and Stemonoporus) possessed certains indels exclusive to each and this may contributed to the monophyletic nature of these genera.
Genetic variations of Lansium domesticum Corr. accessions from Java, Sumatra and Ceram based on Random Amplified Polymorphic DNA fingerprints
Biodiversitas , 2011,
Abstract: Yulita KS (2011) Genetic variations of Lansium domesticum Corr. accessions from Java, Bengkulu and Ceram based on Random Amplified Polymorphic DNA fingerprints. Biodiversitas 12: 125-130. Duku (Lansium domesticum Corr.) is one of popular tropical fruits in SE Asia. The spesies has three varieties, known as duku, langsat and kokosan; and duku is the most popular one for being the sweetiest fruit. Indonesia has several local varieties of duku, such as duku Condet, duku Sumber and duku Palembang. This present study aimed to assess genetic diversity of 47 accessions of duku from Java, Sumatra, and Ceram based on RAPD fingerprints. Ten RAPD’s primers were initially screened and five were selected for the analysis. These five primers (OPA 7, 13, 18, OPB 7, and OPN 12) generated 53 scorable bands with an average of 10.6 polymorphic fragment per primer. Percentage of polymorphism ranged from 16.89% (OPA 7 and OPN 12) to 24.54% (OPB 7) with an average of 20.16% polymorphism. OPB 7 at 450 bp was exclusively possessed by accession 20 (Java), OPA 18 at 500 bp was by accession 6 (Java), 550 bp by 3 clones from Bengkulu. While OPN 12 at 300 bp and OPA 13 at 450 bp were shared among the accessions. Clustering analysis was performed based on RAPD profiles using the UPGMA method. The range of genetic similarity value among accessions was 0.02-0.65 suggesting high variation of gene pool existed among accessions.
The Occurrence of Hybrid in Nepenthes hookeriana Lindl. from Central Kalimantan can be Detected by RAPD and ISSR Markers
HAYATI Journal of Biosciences , 2012,
Abstract: Nepenthes spp. (Nepenthaceae) is one of the most popular ornamental plants in Southeast Asia. There are 97 species of Nepenthes to which 64 are found in Indonesia with the center of its diversity located in Borneo. N. x hookeriana was hypothesised to be a natural hybrid between N. ampullaria and N. rafflesiana on the basis of morphological characters. Several variants of each species were also known. This present study aimed to detect the occurrence of hybrid within N. x hookeriana ‘spotted’ and ‘green’ variant using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR). Five RAPD primers and three ISSR primers were used to amplify total DNA genome and produced 83 polymorphic bands ranging in size from 300-1700 bp. Clustering analysis was performed based on RAPD and ISSR profiles using the UPGMA method. The genetic similarity of the combined markers range between 0.30-0.75 indicating a narrow range of genetic similarity among the accessions. Results from cluster analyses suggested that N. x hookeriana was indeed a hybrid between N. ampullaria and N. Rafflesiana, however it was genetically more similar to N. raflessiana.
A phage display combined with DNA affinity magnetic system can be applied to a screening of DNA binding proteins, such as transcription factors
Yulita,Kusumadewi Sri; Kouno,Takafumi; Ezaki,Bunichi;
Electronic Journal of Biotechnology , 2010,
Abstract: here we introduce a new approach for the screening of dna binding proteins, using a phage library based on a phage display technique. in principal, a complementary dna (cdna) library based on the recombinant bacteriophage t7 expressing target proteins on its capsid (phage display) is constructed. these phage particles are hybridized with a biotinylated target dna fragment which is immobilized on the surface of streptavidin paramagnetic particle (sa-pmp). the phage particles are released from the target dna fragment by a nuclease treatment and the recovered phages are used to the next round of hybridization. these processes are repeated three times to amplify the target phages in the population. this simple method is faster, and more systemic than other current methods (e.g. yeast one hybrid system). as a proof of this principle, we tried to isolate transcription factors which specifically bind to the promoter region of the arabidopsis thaliana atgst11 gene. two obtained candidates, ring zinc finger protein and athb6, showed dna binding activity to the atgst11 promoter region. we could validate that our new application of phage display is a superior method for isolation of dna binding proteins with a broad range of potential applications.
Lusiana Indriasari Sagita,Sri Kusumadewi
Jurnal Informatika , 2012,
Abstract: Munculnya suatu penyakit telah menjadi momok menakutkan yang selalu menghantui manusia. Karena sampai saat ini resiko adanya suatu penyakit masih menjadi penyebab utama kematian seseorang. Untuk mengetahui seseorang dalam keadaan sehat atau sakit, seorang dokter akan melakukan beberapa pendekatan diagnosis terhadap pasien yang salah satunya ialah melalui pemeriksaan laboratorium. Pemeriksaan Laboratorium ini, dilakukan dengan harapan bahwa resiko terhadap suatu penyakit dapat dideteksi lebih dini secara tepat dan akurat. Sistem berbasis kasus atau Case Based Reasoning (CBR) merupakan model penalaran untuk menyelesaikan masalah dengan menggunakan konsep analogi. CBR akan membandingkan suatu kasus baru dengan kasus-kasus lain yag sudah tersimpan sebelumnya. Selain itu, akan dilakukan penandaan terhadap kasus--kasus klinis, serta menemukan kembali kasus-kasus yang mirip. Sistem berbasis kasus untuk diagnosis penyakit melalui pemeriksaan laboratorium merupakan sistem yang dapat menentukan suatu keputusan mengenai diagnosis penyakit melalui hasi pemeriksaan laboratorium dengan menggunakan metode CBR.. Selain diagnosis penyakit, sistem akan memberikan informasi penyebab tinggi rendahnya suatu nilai pemeriksaan dan solusi terapinya. Dengan aplikasi ini diharapkan dapat memberikan manfaat yang lebih untuk melakukan diagnosis penyakit dan proses rekam medik pasien.
Lucie Widowati,Awal Prichatini Kusumadewi,Sri Murhandini
Media of Health Research and Development , 2012,
Abstract: Teratogenic test has been performed on sweet stevia as product of B2P2TO2T cultivation at rats (Rattus novergicus) pregnant female Wistar. Stevia sweet was administered orally at a dose of 360, 120 and 40 mg/kg bw, volume 1 ml/100g bw per day during organogenesis period, on the day of pregnancy to the 6th until 15th. During the test, test animals were observed two times daily with the distance of six hours against the toxicity symptoms such as changes in skin, hair, eyes and mucous membranes, bleeding. Animals that experienced abortion, premature birth or death during the trial period were sacrificed and observed immediately with microscopic technic. At 20th day of pregnancy all of the pregnant rat dissected, and put out the fetuses from the mother's and observated the health conditions in general and the whole of mothers reproductive systems of fetuses, the outer fetal malformation and soft tissue system of fetal. The conclusion were sweet stevia of B2P2TO2T no worse effect on the mother rats, fetal body weight and morphology of mother rats and the fetus, does not affect the process of development of fetal soft tissue, and does not affect the development of fetal skeleton. Generally the B2P2TO2T stevia sweet substances safe for used, does not cause teratogenic effects.
Solving the Fractional Rosenau-Hyman Equation via Variational Iteration Method and Homotopy Perturbation Method
R. Yulita Molliq,M. S. M. Noorani
International Journal of Differential Equations , 2012, DOI: 10.1155/2012/472030
Solving the Fractional Rosenau-Hyman Equation via Variational Iteration Method and Homotopy Perturbation Method
R. Yulita Molliq,M. S. M. Noorani
International Journal of Differential Equations , 2012, DOI: 10.1155/2012/472030
Abstract: In this study, fractional Rosenau-Hynam equations is considered. We implement relatively new analytical techniques, the variational iteration method and the homotopy perturbation method, for solving this equation. The fractional derivatives are described in the Caputo sense. The two methods in applied mathematics can be used as alternative methods for obtaining analytic and approximate solutions for fractional Rosenau-Hynam equations. In these schemes, the solution takes the form of a convergent series with easily computable components. The present methods perform extremely well in terms of efficiency and simplicity. 1. Introduction Recent advances of fractional differential equations are stimulated by new examples of applications in fluid mechanics, viscoelasticity, mathematical biology, electrochemistry, and physics. For example, the nonlinear oscillation of earthquake can be modeled with fractional derivatives [1], and the fluid-dynamic traffic model with fractional derivatives [2] can eliminate the deficiency arising from the assumption of continuum traffic flow. Based on experimental data fractional partial differential equations for seepage flow in porous media are suggested in [3], and differential equations with fractional order have recently proved to be valuable tools to the modeling of many physical phenomena [4]. Fractional partial differential equations also have studied and successfully solved such as the space-time fractional diffusion-wave equation [5–7], the fractional advection-dispersion equation [8, 9], the fractional KdV equation [10], and the linear inhomogeneous fractional partial differential equations [11]. Most nonlinear differential equations are usually arising from mathematical modeling of many physical systems. In most cases, it is very difficult to achieve analytic solutions of these equations. Perturbation techniques are widely used in science and engineering to handle nonlinear problems and do great contribution to help us understand many nonlinear phenomena. However, perturbation techniques are based on the existence of small/large parameter. Therefore, these techniques are not valid for strongly nonlinear problems. The homotopy perturbation method (HPM) is the new approach for finding the approximate analytical solution of linear and nonlinear problems. The method was first proposed by He [12, 13] and was successfully applied to solve nonlinear wave equation by He [14–16]. The convergence of Homotopy perturbation series to the exact solution is considered in [17]. Similarly, applying the variational iteration method,
Degradation of Primate Habitat at Tesso Nilo Forest with Special Emphasis on Riau Pale-Thighed Surili (Presbytis siamensis cana)  [PDF]
Jatna Supriatna, Sri Mariati
Journal of Environmental Protection (JEP) , 2014, DOI: 10.4236/jep.2014.512112
Abstract: The habitat of Riau pale-thighed surili (Presbytis siamensis cana) located in Riau province of Sumatra island between Siak in the north and Indragiri rivers in the south, has been seriously degraded in the last a few decades. This swampy peat land forest has been degraded by frequent burning during the dry season. These problems originated because of poor governance on the forest management, and the construction of two access roads by the owners of logging concessions and wood pulp plantations. This forest block consists of Tesso Nilo National Park, Kerumutan protected area, Production Forest areas belong to two companies, and a small recreation Park. It also contains one of the most important mixed peat swamp forests. Here, we tracked changes in forest cover before and after road construction using GIS and remote sensing imagery. Deforestation rates have increased from 1.5% per year before road construction to 9.28% per year after road construction, which means that the area of forest has decreased by an annual average of 8156 ha in Tesso Nillo alone. The habitat of Riau pale-thighed surili decreased almost up to 60% in the last decade after companies started their converting forest for oil palm and acacia and roads were built crisscrossed. If the trend of deforestation continues, further decline of population of habitat of this species is unavoided. Researches should be carried out in more details to understand the status of population of this subspecies before this subspecies disappears in the wild.
Monolayers Langmuir-Blodgett Films of Synthetic Artificial Mimic Molecules That Resemble the Following Tetraether Lipids on Silicon Wafers  [PDF]
Sri Vidawati, Ulrich Rothe
Advances in Biological Chemistry (ABC) , 2015, DOI: 10.4236/abc.2015.54015
Abstract: This study investigated the behavior and molecular organization of synthetic artificial mimic molecules that resemble the following tetraether lipids: di-O-hexadecyl-glycero-3-phosphatidyl-glycerol (DHGPG) and bis-4-dodecylphenyl-12-phosphate. These molecules were analyzed using Langmuir film balance, ellipsometry and atomic force microscopy. The monolayer Langmuir-Blodgett films of DHGPG and bis-4-dodecylphenyl-12-phosphate were stable on the solid surface silicon wafers. The ellipsometry and AFM results showed that monolayers Langmuir-Blodgett films of DHGPG and bis-4-dodecylphenyl-12-phosphate were present, and the thickness of the observed films varied from 1.2 - 5.0 nm.
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