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Search Results: 1 - 10 of 48007 matches for " KOU Xiao-Xia "
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Detection of waterborne norovirus
水源性诺如病毒

KOU Xiao-Xia,WU Qing-Ping,XUE Liang,
寇晓霞
,吴清平,薛亮

微生物学通报 , 2012,
Abstract: Norovirus is the major cause of acute non-bacterial gastroenteritis worldwide and often lead to sever food safety incidents. Water is one of the important vectors of norovirus transmission. The paper gives an overview about the environmental résistance, concentration, detection and epidemiology of norovirus in water. At the same time, the outstanding problem and developmental direction of norovirus detection is also enclosed.
Foodborne Viruses and its Detection Methods
食源性病毒及其检测方法*

WU Qing-Ping KOU Xiao-Xia ZHANG Ju-Mei,
吴清平
,寇晓霞,张菊梅

微生物学通报 , 2004,
Abstract: Foodbome viruses are defined to be viruses that can lead to human diseases through food. In accordance with the different origin, foodborne viruses can be divided into two kinds: intestinal viruses and zoonotic viruses. The former include those viruses that can be transmitted to person via fecal-orally route. The latter include those zoonotic viruses that chiefly transmitted to person through livestock and poultry products. This paper expounds foodborne viruses categories, biology nature, epidemiology character, and study circumstance, and clarifies the molecular biological methods and problems on the base of the polymerase chain reactions, and presents the development direction and application perspective of the foodbome viruses study.
Development of Noroviruses
诺瓦克病毒研究进展

WANG Da-peng,WU Qing-ping,KOU Xiao-xia,
王大鹏
,吴清平,寇晓霞

微生物学报 , 2007,
Abstract: Noroviruses (NVs) were one of the new borne viruses, which was found firstly in the Unit States of America in 1972 and reported in China in 1995. The main food-borne viral pathogens affect people badly and cause the epidemic acute gastroenteritis in all kinds of people. And to this day, however, no cell lines and animal models have been found, which has hampered the study of these viruses. With the progress of the molecular biology and other subjects, the genomes of different NVs were sequenced, and the proteins of the viruses were expressed in vitro by the eukaryotic and prokaryotic expression systems respectively. Therefore, the novel knowledge and ideas on NVs were developed quickly in the character of these kinds of viruses. In this article, the NVs were described systematically, such as the structure of genomes and function of these nucleotides, organization and function of proteins, application and development of detecting and accumulation of epidemiology. Furthermore, the majority of researchers were interested in and focused on the study of protein and the detection of viruses. The progress and obstacles in this field were also involved. In additional, the suggestions were mentioned about the molecular evolution, detection and multiplication system in vitro on the viruses.
Study on Rotavirus detection by nucleic acid sequence based amplification
轮状病毒NASBA检测研究

KOU Xiao-xia,WU Qing-ping,FAN Hong-ying,
寇晓霞
,吴清平,范宏英

微生物学报 , 2006,
Abstract: Rotavirus is one of the major cause of the viral gastroenteritis throughout the world. A nucleic acid sequence-based amplification(NASBA) technique for the detection of rotavirus in faecal specimens was developed and compared to the RT-PCR technique. Primers were designed according to the high conserved region of VP7 gene. Amplicons were detected by denaturating agarose gel, and then demonstrated by Northern hybridization with digoxigenin-labeled oligonucleotide probe. The anticipative specific amplification product is 392bp,and no nonspecific products appear even the concentration of nontarget nucleic acid as high as 1 microg/microL. A detection limit of 50 pg target RNA/mL is obtained when the optimal amplification time of 3h used.The NASBA assay will be a favourable alternative to RT-PCR for the investigation of rotavirus outbreaks as a routine diagnostic test in the near future because it is shown to be highly sensitive, specific and do not require specialized equipment.
Study on Rotavirus detection with single-tube seminested RT-PCR method in shellfish
单管半套式RT-PCR法检测贝类中轮状病毒的研究

KOU Xiao-xia,WU Qing-Ping,ZHANG Jv-mei,FAN Hong-ying,
寇晓霞
,吴清平,张菊梅,范宏英

微生物学报 , 2005,
Abstract: Being a foodborne virus, Rotavirus was often carried by shellfish. At the present, RT_PCR was the most effective method of Rotavirus detection in shellfish, but its sensitivity was low because of low levels of virus contamination and PCR inhibitors in shellfish. So contaminated shellfish experimentally in laboratory,and imitated the natural environment to concentrate Rotavirus, then detected by the developed single_tube seminested RT_PCR. Compared with ELISA and the conventional RT_PCR, the detection sensitivity is improved by 1000 times and 10 times respectively. In addition, the outer and inner contamination is reduced dramatically and the detection time is decreased from 6h to 4.5h. When the whole shellfish and only the digestive tissues of shellfish serve as samples,the detection ratio and sensitivity are more higher when sample is the latter.
An Approach to Transforming from CIM to PIM Using Pattern
一种运用模式将CIM转换到PIM的方法

CAO Xiao-Xia,MIAO Huai-Kou,SUN Jun-Mei,
曹晓夏
,缪淮扣,孙军梅

计算机科学 , 2007,
Abstract: 模型转换在MDA软件开发方法中扮演着非常重要的角色,尤其是从CIM到PIM的转换。本文给出了一种从CIM转换到PIM的方法。在CIM中,我们通过特征模型来组织需求,同时用软件体系结构来组织PIM中的各个要素。这个转换中的核心内容是模式的应用。在CIM的需求模型中,本文将特征分层,从而将需求分为不同的层次。同时模式也被分为不同的层级,其中包括体系结构模式和设计模式。针对不同层级的特征模型,应用不同层级的模式进行变换,从而得到分层的体系结构。当需求发生变化时,首先确定这种特征的变化是在哪个层级上的,然后在不同的体系结构层级上变换相应的功能,从而实现PIM的相应变化。本文最后以自行开发的Object-Z的支持工具为例来说明所给出的方法。
Ethyl 2-(1H-1,2,3-benzotriazol-1-yl)acetate
Xiao-Xia Li,Zhong Chen
Acta Crystallographica Section E , 2011, DOI: 10.1107/s160053681005138x
Abstract: The title compound, C10H11N3O2, was synthesized by the reaction of 1H-benzotriazole with ethyl 2-chloroacetate in ethanol. The non-H atoms, excluding the benzotriazol-1-yl group, are almost coplanar (r.m.s. deviation of the non-H atoms = 0.0409 ). The dihedral angle formed between this plane and the benzotriazole ring is 79.12 (5)° In the crystal, weak intermolecular C—H...N and C—H...O interactions help to consolidate the three-dimensional network.
4-(4-Chlorobenzoyl)-3-methyl-1-phenyl-1H-pyrazol-5-yl 4-chlorobenzoate
Xiao-Xia Li,Zhong Chen
Acta Crystallographica Section E , 2011, DOI: 10.1107/s1600536811048136
Abstract: In the title compound, C24H16Cl2N2O3, the three benzene rings are twisted with respect to the central pyrazole ring, making dihedral angles of 71.56 (9) (4-chlorobenzoyloxy), 57.55 (8) (4-chlorobenzoyl) and 39.33 (1)° (phenyl).
Supersymmetric Two-Boson Equation: Bilinearization and Solutions
Q. P. Liu,Xiao-Xia Yang
Physics , 2005, DOI: 10.1016/j.physleta.2005.10.075
Abstract: A bilinear formulation for the supersymmetric two-boson equation is derived. As applications, some solutions are calculated for it. We also construct a bilinear Backlund transformation.
Expression of the ORF2 of Norovirus in Escherichia coli and the Product Analysis
诺瓦克病毒ORF2的原核表达及其产物分析

WANG Da-Peng,WU Qing-Ping,YAO Lin,KOU Xiao-Xia,ZHANG Ju-Mei,
王大鹏
,吴清平,姚 琳,寇晓霞,张菊梅

微生物学通报 , 2008,
Abstract: Norovirus (NV) was one of new borne viruses, which was found firstly in the USA in 1972 and not reported in China until 1995. The main food-borne viral pathogens affect people badly and cause the epidemic acute gastroenteritis in all age groups worldwide. In this study, the genomic RNA was extracted from the non-bacterial gastroenteritis samples. A 1623 bp fragment, containing the complete coding sequence of the ORF2 gene, was amplified from the NV samples by RT-PCR. Sequencing analysis showed that it belongs to GII and shared more than 99% homology with the corresponding sequences published in the GenBank(DQ419908 and DQ369797). The ORF2 gene was then in-frame fused to the prokaryotic expression vector pET-28a, and the resultant recombinant plasmid was transformed into Escherichia coli BL21 (DE3) competent cells. A 62 kD fusion protein, named rVP1, was expressed after IPTG induction. Rabbits were immunized by the purified rVP1. Western Blot results showed that the high titer antibody can specifically recognize the VP1 in the clinical samples from hospital. The data suggested that the rVP1 has good immunogenicity and reactiongenicity. The minor protein in the expression product was analyzed by Western Blot and double?immunodiffusion?test. The data showed that the minor proteins were the fragments of rVP1.
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