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Search Results: 1 - 10 of 36614 matches for " KANG Qiang-Sheng "
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Construction of a Plant Transgenic Expression Vector of Peanut

WANG Jing,YAN Hai-Yan,KANG Qiang-Sheng,

植物科学学报 , 2005,
Abstract: 通过用一对特异性引物,PCR扩增得到花生(Arachis hypogaea)主要致敏蛋白Arah1的基因的启动子片段1957bp,其序列组成与已发表序列基本一致。用其替换pBIN 35S-mGFP4载体中的35S启动子部分,组成一种花生转基因表达载体pGA1。该载体含有种子特异表达调控元件、增强表达元件和一些与转录因子结合的调控元件,是一个强启动子,便于将外源基因转入花生,实现外源基因在花生中的表达,为建立花生生物反应器奠定了基础。
Dual Application of Arbuscular Mycorrhizal Fungi and Polyamines Affects Growth and Nutrient Uptake of Trifoliate Orange (Poncirus trifoliata) Seedlings
Qiang-Sheng WU,Ying-Ning ZOU
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2009,
Abstract: The experiment was carried out to study the dual application effects of Arbuscular Mycorrhizal Fungi (AMF) and polyamines on growth and nutrient uptake of trifoliate orange (Poncirus trifoliata) seedlings. The seedlings were colonized by Glomus versiforme and irrigated with 320 mL 100 mg/L putrescine, spermidine and spermine, respectively. Two months after exogenous polyamines treatments, both putrescine and spermine applications significantly increased the mycorrhizal colonization, whereas spermidine supplement did not alter the colonization rate. The sole AMF inoculation significantly increased total dry weight, leaf P, K, Ca, Mg, Fe, Cu and Mn contents and root P, K, Ca, Fe, Cu and Zn contents of the seedlings, compared to the non-AMF control. Compared to the sole AMF inoculation, additional putrescine and spermine markedly increased total dry weight, and elevated leaf P and K contents and root P, Mg, Fe and Zn contents. These increases were more significantly in the mycorrhizal seedlings supplied with putrescine than in the mycorrhizal seedlings supplied with spermine. All these polyamines applications did not affect root Cu and Mn contents, but enhanced leaf Mn uptake and root Ca uptake. Spermidine treatment had almost no effects on nutrient uptake and growth of the seedlings. These results suggest that dual application of G. versiforme and putrescine could be a feasible procedure for better citrus cultivation.
Sodium Chloride Stress Induced Changes in Leaf Osmotic Adjustment of Trifoliate Orange (Poncirus trifoliata) Seedlings Inoculated with Mycorrhizal Fungi
Ying-Ning ZOU,Qiang-Sheng WU
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2011,
Abstract: Citrus plants are sensitive to salinity, and thus employing new approaches to alleviate salt damage are necessary. The present study evaluated the effect of two arbuscular mycorrhizal fungi (AMF), Glomus mosseae and G. versiforme, on leaf osmotic adjustment of trifoliate orange (Poncirus trifoliata) seedings exposed to 100 mM NaCl. Salinity significantly inhibited mycorrhizal colonization, plant biomass and leaf relative water content, whereas the reduce of plant biomass was notably alleviated by the mycorrhizal colonization. Mycorrhizal seedlings exhibited significantly lower Na+ and Ca2+ concentrations, whilst also recorded higher K+ concentration and K+/Na+, Ca2+/Na+ and Mg2+/Na+ ratios at both salinity levels. Under salinity stress, mycorrhizal symbiosis markedly decreased sucrose concentrations of leaves and also increased glucose, fructose and proline concentrations of leaves. The results suggest that arbuscular mycorrhizas improved leaf osmotic adjustment responses of the seedlings to salt stress, thus enhancing salt tolerance of mycorrhizal plants.
Improvement of Root System Architecture in Peach (Prunus persica) Seedlings by Arbuscular Mycorrhizal Fungi, Related to Allocation of Glucose/Sucrose to Root
Qiang-Sheng WU,Guo-Huai LI,Ying-Ning ZOU
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2011,
Abstract: Root system architecture (RSA) is used to describe the spatial configuration of a root system in the soil, which substantially determines the capacity of a plant to take up nutrients and water. The present study was to assess if arbuscular mycorrhizal fungi (AMF), Glomus mosseae, G. versiforme, and Paraglomus occultum would alter RSA of peach (Prunus persica L. Batsch) seedlings, and the alteration due to mycorrhization was related to allocation of glucose/sucrose to root (Aglucose/sucrose). Inoculation with G. mosseae and G. versiforme significantly increased leaf, stem, root and total fresh weights, compared with non-AMF treatment. Mycorrhizal alterations of RSA in peach plants were dependent on AMF species, because only G. mosseae and G. versiforme but not P. occultum markedly increased root length, root projected area, root surface area and root volume. For the distribution of root length classes, AMF mainly increased 0-1 and 3-4 cm root length classes, which is AMF species dependent. Inoculated seedlings with Glomus species recorded significantly higher root sucrose and leaf and root glucose concentrations and lower root sucrose concentrations than un-inoculated control. Compared with the non-AMF treatment, G. mosseae and G. versiforme generally increased the Aglucose and Asucrose, but P. occultum significantly decreased the Aglucose and Asucrose. Asucrose or Aglucose was significantly positive correlated with root length, root projected area and root surface area. The results suggest that AMF modified variables of RSA in peach, which is AMF species dependent and related to Aglucose and Asucrose.
Polyamines Participate in Mycorrhizal and Root Development of Citrus (Citrus tangerine) Seedlings
Qiang-Sheng WU,Ying-Ning ZOU,Tian-Tian ZHAN,Chun-Yan LIU
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2010,
Abstract: A pot experiment was conducted to study the effects of difluoromethylornithine (DFMO, an irreversible inhibitor of putrescine (Put) formation) alone or combination with Put on mycorrhizal and root development of Citrus tangerine seedlings inoculated with Glomus mosseae. DFMO significantly inhibited plant growth (plant height, stem diameter, leaf number per plant, shoot and root dry weights), mycorrhizal (root colonization and the number of entry point, vesicle and arbuscule) and root development (total length, total projected area, total surface area and total volume), chlorophyll content, photosynthesis (photosynthetic rates, transpiration rates and stomatal conductance) and soluble protein, whereas the inhibition was partly or completely reversed by Put application to DFMO treatment. DFMO applied to mycorrhizal seedlings showed the decrease of P contents of leaves and roots, the decrease of acid, neutral and alkaline phosphatase activities of rhizosphere and the increase of available P content of rhizosphere, whereas application of exogenous Put to DFMO treatment partly unchained the effects of DFMO. DFMO strongly inhibited the allocation of glucose to root but stimulated the allocation of sucrose to root and guaiacol peroxidase (G-POD) activity of root, but these effects were reversed to a certain extent when Put was included in DFMO treatment. This study thus indicates that endogenous polyamines regulate mycorrhizal development of citrus seedlings through altering the allocation of carbohydrates to root, and also participate in root development, which is negatively related to root G-POD and may be due to inducing new protein synthesis.
Effects of Exogenous Putrescine on Mycorrhiza, Root System Architecture, and Physiological Traits of Glomus mosseae-Colonized Trifoliate Orange Seedlings
Qiang-Sheng WU,Ying-Ning ZOU,Min LIU,Kun CHENG
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2012,
Abstract: Putresince (Put) as one of the important polyamines (PAs) has been identified to regulate mycorrhizal development of citrus plants. The present study was to screen an efficient concentration of Put application at the range of 0.05-1 mM on the trifoliate orange (Poncirus trifoliata) seedlings colonized by Glomus mosseae, in terms of growth, root system architecture, and chlorophyll and carbohydrate contents. Compared to the non-Put treatment, all the Put treatments, especially 0.05 mM Put, significantly increased mycorrhizal colonization of tap root in addition to first, second, and third order lateral roots. The mycorrhizal seedlings treated by 0.05, 0.1, and 1 mM Put showed greater growth (stem diameter, height, leaf number, and fresh mass) and root morphological properties ( tap root length, projected and surface areas, and volume) and higher numbers of first, second, and third order lateral roots. Bio-molecules like chlorophyll a, total chlorophyll, and carotenoid contents of the seedlings were significantly increased by the Put treatments at 0.05-1 mM. All exogenous Put application at the range of 0.05-1 mM significantly decreased sucrose contents but increased glucose contents of leaves and roots. This study suggests that exogenous Put can significantly improve growth performance and root system architecture, besides changes in physiological traits of AMF seedlings. The 0.05 mM concentration of Put showed the best effects.
Effects of arbuscular mycorrhizal fungi on reactive oxygen metabolism of Citrus tangerine leaves under water stress.

WU Qiang-sheng,ZOU Ying-ning,XIA Ren-xue,

应用生态学报 , 2007,
Abstract: In a pot experiment, this paper studied the effects of arbuscular mycorrhizal (AM) fungus Glomus versiforme (Karsten) Berch inoculation on the reactive oxygen metabolism of Citrus tangerine Hort. ex Tanaka leaves under water stress. The results showed that water stress decreased the colonization of G. versiforme on C. tangerine roots significantly, with a decrement of 33%. Under normal water supply and water stress, G. versiforme inoculation increased the leaf P content by 45% and 27%, and decreased the leaf malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents by 25% and 21%, and 16% and 16%, respectively, compared with the control. Inoculation with G. versiforme enhanced the activities of leaf superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), and increased the contents of leaf soluble protein, ascorbate (ASC) and total ascorbate (TASC) notably, regardless soil moisture condition. Under water stress, G. versiforme inoculation decreased the leaf superoxide anion radical (O2-*) content by 31%, compared with that under normal water supply. It was suggested that the drought resistance of C. tangerine leaves was enhanced after G. versiforme inoculation.
Mycorrhizal-Mediated Lower Proline Accumulation in Poncirus trifoliata under Water Deficit Derives from the Integration of Inhibition of Proline Synthesis with Increase of Proline Degradation
Ying-Ning Zou, Qiang-Sheng Wu, Yong-Ming Huang, Qiu-Dan Ni, Xin-Hua He
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0080568
Abstract: Proline accumulation was often correlated with drought tolerance of plants infected by arbuscular mycorrhizal fungi (AMF), whereas lower proline in some AM plants including citrus was also found under drought stress and the relevant mechanisms have not been fully elaborated. In this study proline accumulation and activity of key enzymes relative to proline biosynthesis (?1-pyrroline-5-carboxylate synthetase, P5CS; ornithine-δ-aminotransferase, OAT) and degradation (proline dehydrogenase, ProDH) were determined in trifoliate orange (Poncirus trifoliata, a widely used citrus rootstock) inoculated with or without Funneliformis mosseae and under well-watered (WW) or water deficit (WD). AMF colonization significantly increased plant height, stem diameter, leaf number, root volume, biomass production of both leaves and roots and leaf relative water content, irrespectively of water status. Water deficit induced more tissue proline accumulation, in company with an increase of P5CS activity, but a decrease of OAT and ProDH activity, no matter whether under AM or no-AM. Compared with no-AM treatment, AM treatment resulted in lower proline concentration and content in leaf, root, and total plant under both WW and WD. The AMF colonization significantly decreased the activity of both P5CS and OAT in leaf, root, and total plant under WW and WD, except for an insignificant difference of root OAT under WD. The AMF inoculation also generally increased tissue ProDH activity under WW and WD. Plant proline content significantly positively correlated with plant P5CS activity, negatively with plant ProDH activity, but not with plant OAT activity. These results suggest that AM plants may suffer less from WD, thereby inducing lower proline accumulation, which derives from the integration of an inhibition of proline synthesis with an enhancement of proline degradation.
Effects of exogenous ABA and GA3 on sugar concentration in flesh of Cara Cara Navel Orange

WANG Gui-yuan,XIA Ren-xue,ZENG Xiang-guo,WU Qiang-sheng,

应用生态学报 , 2007,
Abstract: The concentration of glucose, fructose, sucrose and total sugar were determined after exogenous ABA and GA3 treatment during young period of fruit and before fruit coloring in flesh of Cara Cara Navel Orange. The results showed that 10 mg x L(-1) ABA treatment improved glucose, fructose and total sugar concentration significantly or very significantly, 50 mg x L(-1) ABA treatment improved sucrose concentration very significantly, but 100 mg x L(-1) ABA treatment reduced glucose concentration very significantly. GA3 treatment of lower and middle concentrations (10, 50 and 250 mg x L(-1)) improved sucrose concentration very significantly, 10 mg x L(-1) GA3 treatment had no remarkable effect on glucose and fructose concentration but improved total sugar concentration very significantly, GA3 treatment of 50, 250 and 500 mg x L(-1) decreased glucose, fructose and total sugar concentration very significantly. Therefore, ABA treatment of lower concentration could improve one or several kinds of sugar concentration, but GA3 treatment of higher concentration (250 and 500 mg x L(-1)) prohibited sugar accumulation in flesh of Cara Cara Navel Orange seriously.
Strong stem effect and physiological characteristics of rice plant under rice-duck farming.

WANG Qiang-sheng,ZHEN Ruo-hong,DING Yan-feng,WANG Shao-hua,

应用生态学报 , 2008,
Abstract: A field plot comparison experiment was conducted to study the strong stem effect and physiological characteristics of rice plant under rice-duck farming. The results showed that under rice-duck farming, the morphology of rice plant changed obviously, and the carbohydrate content and C/N ratio of the plant as well as the dry matter output from rice stem increased significantly, due to the activities of duck in paddy field. In the meantime, the length of basal internodes decreased by 2.88%, while the stem diameter, stem mechanical strength, and stem lodging resistant index increased by 64.90%, 11.78%, and 10.95%, respectively. Rice-duck farming increased the root mass and root vitality in deeper soil layers, and decreased the proportion of black roots by 16.63%. It was indicated that rice-duck farming benefited the formation of strong rice stem and increased the stress resistance of rice plant, which would be favorable to the stable and high-yielding of rice.
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