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Search Results: 1 - 10 of 5062 matches for " Jung Gerlinde "
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Side chain modified peptide nucleic acids (PNA) for knock-down of six3 in medaka embryos
Dorn Sebastian,Aghaallaei Narges,Jung Gerlinde,Bajoghli Baubak
BMC Biotechnology , 2012, DOI: 10.1186/1472-6750-12-50
Abstract: Background Synthetic antisense molecules have an enormous potential for therapeutic applications in humans. The major aim of such strategies is to specifically interfere with gene function, thus modulating cellular pathways according to the therapeutic demands. Among the molecules which can block mRNA function in a sequence specific manner are peptide nucleic acids (PNA). They are highly stable and efficiently and selectively interact with RNA. However, some properties of non-modified aminoethyl glycine PNAs (aegPNA) hamper their in vivo applications. Results We generated new backbone modifications of PNAs, which exhibit more hydrophilic properties. When we examined the activity and specificity of these novel phosphonic ester PNAs (pePNA) molecules in medaka (Oryzias latipes) embryos, high solubility and selective binding to mRNA was observed. In particular, mixing of the novel components with aegPNA components resulted in mixed PNAs with superior properties. Injection of mixed PNAs directed against the medaka six3 gene, which is important for eye and brain development, resulted in specific six3 phenotypes. Conclusions PNAs are well established as powerful antisense molecules. Modification of the backbone with phosphonic ester side chains further improves their properties and allows the efficient knock down of a single gene in fish embryos.
The patatin-like lipase family in Gallus gallus
Jani Saarela, Gerlinde Jung, Marcela Hermann, Johannes Nimpf, Wolfgang J Schneider
BMC Genomics , 2008, DOI: 10.1186/1471-2164-9-281
Abstract: We scanned the draft chicken genome using human PNPLA sequences, and performed PCR to amplify and sequence orthologous cDNAs. Full-length cDNA sequences of galline PNPLA2/ATGL, PNPLA4, -7, -8, -9, and the activator protein CGI-58, as well as partial cDNA sequences of avian PNPLA1, -3, and -6 were obtained. The high degree of sequence identities (~50 to 80%) between the avian and human orthologs suggests conservation of important enzymatic functions. Quantitation by qPCR of the transcript levels of PNPLAs and CGI-58 in 21 tissues indicates that expression patterns and levels diverge greatly between species. A particularly interesting tissue in which certain PNPLAs may contribute to physiological specialization is the extraembryonic yolk sac.Knowledge about the exact in-vivo functions of PNPLAs in any system is still sparse. Thus, studies about the temporal expression patterns and functions of the enzymes identified here, and of other already known extracellular lipases and co-factors, in the yolk sac and embryonic tissues during embryogenesis are called for. Based on the information obtained, further studies are anticipated to provide important insights of the roles of PNPLAs in the yolk sac and embryo development.In egg-laying species, the oocyte's yolk is the major source of nutrients and regulatory factors for the developing embryo. The accumulation of yolk is predominantly the result of massive transport of lipids and proteins from the liver to oocytes situated within ovarian follicles [1]. In mature hens of the domesticated chicken (Gallus gallus), fully-grown oocytes contain up to 5 g triacylglycerols (TG) and 240 mg cholesterol, and ovulate every 25 h. In parallel to this oocyte-targeted flow, lipid homeostasis of somatic tissues including the follicular cell layers is maintained. Subsequent to fertilization of the oocyte, normal embryo growth likewise depends on efficient nutrient transfer, i.e., mainly of lipid components, from the yolk into the embryonic ci
MOSEP – More Self-Esteem With My E-Portfolio Development of a Train-the-Trainer Course for E-Portfolio Tutors
Wolf Hilzensauer,Gerlinde Buchberger
International Journal of Emerging Technologies in Learning (iJET) , 2009, DOI: 10.3991/ijet.v4i1.820
Abstract: E-portfolios are known as a technology- supported learning method for the documentation of competency development. In this article the didactic approach, the course design and the results of the Leonardo da Vinci project MOSEP (More self-esteem with my e-portfolio) are described. The main objective of the project was to develop, test and evaluate a new e-portfolio training concept for teachers and tutors in order to support learners during their competence development phase.
The Progamic Phase in High-Mountain Plants: From Pollination to Fertilization in the Cold
Gerlinde Steinacher,Johanna Wagner
Plants , 2013, DOI: 10.3390/plants2030354
Abstract: In high-mountains, cold spells can occur at any time during the growing season and plants may be covered with snow for several days. This raises the question to what extent sexual processes are impaired by low temperatures. We tested pollen performance and fertilization capacity of high-mountain species with different elevational distribution in the European Alps ( Cerastium uniflorum, Gentianella germanica, Ranunculus glacialis, R. alpestris, Saxifraga bryoides, S. caesia, S. moschata) during simulated cold snaps in the laboratory. Plants were exposed to 0 °C (the temperature below the snow) for 12, 36, 60 and 84 h. In S. caesia, the experiment was verified in situ during a cold snap. Sexual processes coped well with large temperature differences and remained functional at near-freezing temperatures for a few days. During the cooling-down phase a high percentage (67–97%) of pollen grains germinated and grew tubes into the style. At zero degrees, tube growth continued slowly both in the laboratory and in situ below the snow. Fertilization occurred in up to 100% of flowers in the nival species and in G. germanica, but was strongly delayed or absent in the alpine species. During rewarming, fertilization continued. Overall, progamic processes in high-mountain plants appear fairly robust toward weather extremes increasing the probability of successful reproduction.
Clinical review: The role of the intensive care physician in mass casualty incidents: planning, organisation, and leadership
Peter J Shirley, Gerlinde Mandersloot
Critical Care , 2008, DOI: 10.1186/cc6876
Abstract: 7 July 2005 witnessed the first suicide bombing on the UK mainland, targeting the public in London. The multiple bomb explosions on the London Underground and bus transport system resulted in the biggest mass casualty incident in the UK since the Second World War (52 dead and more than 700 injured). This was preceded by a similar attack in Madrid in 2004 and followed by an attack in Mumbai in 2006, also targeting transport infrastructure.The Royal London Hospital (RLH) is a teaching hospital and major trauma centre, with a 16-bed intensive care unit (ICU), supplemented by a 6-bed surgical high-dependency unit (HDU). On the day of the bombings, 194 casualties were seen at the hospital, of whom 27 were admitted; 7 casualties requiring ventilatory support were admitted to the ICU and the remainder to the trauma ward and HDU. All of the intensive care patients had been triaged directly to the operating theatre to undergo major surgery, including limb amputations, thoracotomy, laparotomy, and craniotomy. After surgery, the patients were transferred to the ICU, from which further assessments (including secondary and tertiary trauma surveys) were organised. The bombing of the London transport network, while highlighting some good practices in our major incident planning, also exposed weaknesses already described by others [1,2].It seems clear to us, in reflecting on our response to the events and the ensuing critical care requirements, that intensivists should be intimately involved in disaster planning and in the initial stages of the response to any major incident. This paper, based in part on our participation with the UK Department of Health expert symposium convened during June 2007, will discuss how this might occur and at what stages of these processes.Five senior intensive care doctors were in the hospital at the time the major incident was declared, and two more subsequently were able to attend. (Of this total, six were anaesthetists and one a physician.) None of
Expression of yeast lipid phosphatase Sac1p is regulated by phosphatidylinositol-4-phosphate
Andreas Kn?dler, Gerlinde Konrad, Peter Mayinger
BMC Molecular Biology , 2008, DOI: 10.1186/1471-2199-9-16
Abstract: Here we show that the expression of lipid phosphatase Sac1p in the yeast Saccharomyces cerevisiae is regulated in response to changes in phosphatidylinositol-4-phosphate (PI(4)P) concentrations. Unlike genes encoding enzymes involved in phospholipid biosynthesis, expression of the SAC1 gene is independent of inositol levels. We identified a novel 9-bp motif within the 5' untranslated region (5'-UTR) of SAC1 that is responsible for PI(4)P-mediated regulation. Upregulation of SAC1 promoter activity correlates with elevated levels of Sac1 protein levels.Regulation of Sac1p expression via the concentration of its major substrate PI(4)P ensures proper maintenance of compartment-specific pools of PI(4)P.Phosphorylated derivatives of phosphatidylinositol, collectively called phosphoinositides, play essential roles in a wide range of cellular processes situated at intracellular membranes [1]. Recent evidence indicates that phosphoinositides are not only short-lived signals that activate downstream regulatory networks, but also play constitutive roles in organelle identity and membrane dynamics [2]. A key property of individual phosphoinositides is their precisely regulated compartment-specific localization [2,3]. The control and maintenance of diverse intracellular phosphoinositide pools is achieved through the functional interplay of specific sets of lipid kinases and phosphatases. Although it has been established that deficiencies in certain lipid phosphatases can lead to severe human disease [4], it is unknown as to how the expression of these enzymes is regulated. In contrast, the transcriptional regulation of enzymes involved in the biosynthesis of major membrane phospholipids is well characterized [5]. The cellular concentrations of metabolic intermediates required for phospholipid biosynthesis, such as inositol, choline and phosphatidic acid, determine the levels of expression of their respective biosynthetic enzymes [6,7]. However, whether the expression of lipid ph
Prevention of alcohol misuse among children, youths and young adults
Korczak, Dieter,Steinhauser, Gerlinde,Dietl, Markus
GMS Health Technology Assessment , 2011,
Abstract: Background: Despite many activities to prevent risky alcohol consumption among adolescents and young adults there is an increase of alcohol intoxications in the group of ten to twenty year old juveniles. Objectives: This report gives an overview about the recent literature as well as the German federal prevention system regarding activities concerning behavioral and policy prevention of risky alcohol consumption among children, adolescents and young adults. Furthermore, effective components of prevention activities are identified and the efficiency and efficacy of ongoing prevention programs is evaluated. Methods: A systematic literature review is done in 34 databases using Bool’sche combinations of the key words alcohol, prevention, treatment, children, adolescents and young adults. Results: 401 studies were found and 59 studies were selected for the health technology assessment (HTA). Most of the studies are done in USA, nine in Germany. A family strengthening program, personalized computer based intervention at schools, colleges and universities, brief motivational interventions and policy elements like increase of prices and taxes proved effective. Discussion: Among the 59 studies there are three meta-analyses, 15 reviews, 17 randomized controlled trials (RCT) and 18 cohort studies. Despite the overall high quality of the study design, many of them have methodological weaknesses (missing randomization, missing or too short follow-ups, not clearly defined measurement parameters). The transferability of US-results to the German context is problematic. Only a few prevention activities reach a sustainable reduction of frequency and/or amount of alcohol consumption. Conclusion: The HTA-report shows the need to develop specific and target group focused prevention activities for the German situation. Essential for that is the definition of target goals (reduction of consumption, change of behaviour) as well as the definition and empirical validation of risky alcohol consumption. The efficacy of prevention activities should be proven before they are launched. At present activities for the reduction or prevention of risky alcohol consumption are not sufficiently evaluated in Germany concerning their sustainable efficacy.
Effectiveness of programmes as part of primary prevention demonstrated on the example of cardiovascular diseases and the metabolic syndrome
Korczak, Dieter,Dietl, Markus,Steinhauser, Gerlinde
GMS Health Technology Assessment , 2011,
Abstract: Background: The HTA-report (HTA = Health Technology Assessment) deals with the primary prevention of cardiovascular diseases and diabetes mellitus type 2. In 2009 approximately 356,000 people died in Germany due to cardiovascular diseases. According to estimations about 6.3 million people are suffering from diabetes mellitus type 2. The interventions that are subsidized by the public health insurance are mainly focused on sufficient physical activities, healthy nutrition, stress management and the reduction of the consumption of addictive drugs and luxury food. Objectives: Which lifestyle-related measures and/or programmes for primary prevention of cardiovascular diseases and of the metabolic syndrome are effective? To what extent will the health status be improved by these offers? To what extent will existing health resources and skills be strengthened by these offers? Are there any differences regarding the effectiveness among the interventions with respect to different settings or subgroups? Which lifestyle-related interventions and/or programmes for primary prevention of cardiovascular diseases and of the metabolic syndrome are sustainable and cost-effective? Which outcome parameters are in the view of the contributors decisive for the evaluation of the effectiveness? In the view of the contributor are there different values between the outcome parameters? In the view of the payers and other actors are there different values between the outcome parameters? Which ethical and juridical factors have to be considered? Which social and/or socio-economic parameters influence the use of the services and effectiveness? Methods: A systematic literature research is done in 35 databases. For the period 2005 to 2010, reviews, epidemiological and clinical studies as well as economical evaluations which deal with primary prevention programmes regarding cardiovascular diseases or the metabolic syndrome are included. Results: 44 publications meet the inclusion criteria. These studies confirm the effectiveness of the primary prevention programmes. Physical activity programs seem to have a stronger effect than nutrition programmes. Psychological programmes prove as well effectiveness, if they include cognitive behaviour therapy. The identified economical studies indicate that programmes for cardiovascular prevention can be conducted cost-effectively. Interventions that focus on the general population turn out to be particularly cost-effective and sustainable. Discussion: There is a wide range of primary preventive effective lifestyle-related interventions with high ev
Effectiveness of geriatric rehabilitation among patients with secondary diagnosis dementia [Effektivit t der ambulanten und station ren geriatrischen Rehabilitation bei Patienten mit der Nebendiagnose Demenz]
Korczak, Dieter,Steinhauser, Gerlinde,Kuczera, Carmen
GMS Health Technology Assessment , 2012, DOI: 10.3205/hta000105
Abstract: [english] Often geriatric patients with secondary diagnosis dementia do not receive a rehabilitation treatment, although it is an obligatory service offered by the statutory health insurance. Scientists have examined the benefit of relevant measures for patients who are slightly or moderately affected with dementia. Thus, also these patients would profit by a rehabilitation. [german] Geriatrische Patienten mit Nebendiagnose Demenz erhalten oft keine Rehabilitation, obwohl diese zu den Pflichtleistungen der gesetzlichen Krankenkasse geh rt. Wissenschaftler haben untersucht, ob und wenn ja, welchen Nutzen entsprechende Ma nahmen für leicht bis moderat demenzkranke Patienten haben. Demnach profitieren durchaus auch diese Patienten von einer Rehabilitation.
Enriched childhood experiences moderate age-related motor and cognitive decline
Megan J. Metzler,Gerlinde A. Metz
Frontiers in Behavioral Neuroscience , 2013, DOI: 10.3389/fnbeh.2013.00001
Abstract: Aging is associated with deterioration of skilled manual movement. Specifically, aging corresponds with increased reaction time, greater movement duration, segmentation of movement, increased movement variability, and reduced ability to adapt to external forces and inhibit previously learned sequences. Moreover, it is thought that decreased lateralization of neural function in older adults may point to increased neural recruitment as a compensatory response to deterioration of key frontal and intra-hemispheric networks, particularly of callosal structures. However, factors that mediate age-related motor decline are not well understood. Here we show that music training in childhood is associated with reduced age-related decline of bimanual and unimanual motor skills in a MIDI keyboard motor learning task. Compared to older adults without music training, older adults with more than a year of music training demonstrated proficient bimanual and unimanual movement, evidenced by enhanced speed and decreased movement errors. Further, this group demonstrated significantly better implicit learning in the weather prediction task, a non-motor task. The performance of older adults with music training in those tasks was comparable to young adults. Older adults, however, displayed greater verbal ability compared to young adults irrespective of a past history of music training. Our results indicate that music training early in life may reduce age-associated decline of neural motor and cognitive networks.
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