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Search Results: 1 - 10 of 46584 matches for " June-Tai Wu "
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Isotope Label-Aided Mass Spectrometry Reveals the Influence of Environmental Factors on Metabolism in Single Eggs of Fruit Fly
Te-Wei Tseng, June-Tai Wu, Yu-Chie Chen, Pawel L. Urban
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0050258
Abstract: In order to investigate the influence of light/dark cycle on the biosynthesis of metabolites during oogenesis, here we demonstrate a simple experimental protocol which combines in-vivo isotopic labeling of primary metabolites with mass spectrometric analysis of single eggs of fruit fly (Drosophila melanogaster). First, fruit flies were adapted to light/dark cycle using artificial white light. Second, female flies were incubated with an isotopically labeled sugar (13C6-glucose) for 12 h – either during the circadian day or the circadian night, at light or at dark. Third, eggs were obtained from the incubated female flies, and analyzed individually by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS): this yielded information about the extent of labeling with carbon-13. Since the incorporation of carbon-13 to uridine diphosphate glucose (UDP-glucose) in fruit fly eggs is very fast, the labeling of this metabolite was used as an indicator of the biosynthesis of metabolites flies/eggs during 12-h periods, which correspond to circadian day or circadian night. The results reveal that once the flies adapted to the 12-h-light/12-h-dark cycle, the incorporation of carbon-13 to UDP-glucose present in fruit fly eggs was not markedly altered by an acute perturbation to this cycle. This effect may be due to a relationship between biosynthesis of primary metabolites in developing eggs and an alteration to the intake of the labeled substrate – possibly related to the change of the feeding habit. Overall, the study shows the possibility of using MALDI-MS in conjunction with isotopic labeling of small metazoans to unravel the influence of environmental cues on primary metabolism.
The COP9 Signalosome Converts Temporal Hormone Signaling to Spatial Restriction on Neural Competence
Yi-Chun Huang,Yu-Nung Lu,June-Tai Wu,Cheng-Ting Chien ,Haiwei Pi
PLOS Genetics , 2014, DOI: doi/10.1371/journal.pgen.1004760
Abstract: During development, neural competence is conferred and maintained by integrating spatial and temporal regulations. The Drosophila sensory bristles that detect mechanical and chemical stimulations are arranged in stereotypical positions. The anterior wing margin (AWM) is arrayed with neuron-innervated sensory bristles, while posterior wing margin (PWM) bristles are non-innervated. We found that the COP9 signalosome (CSN) suppresses the neural competence of non-innervated bristles at the PWM. In CSN mutants, PWM bristles are transformed into neuron-innervated, which is attributed to sustained expression of the neural-determining factor Senseless (Sens). The CSN suppresses Sens through repression of the ecdysone signaling target gene broad (br) that encodes the BR-Z1 transcription factor to activate sens expression. Strikingly, CSN suppression of BR-Z1 is initiated at the prepupa-to-pupa transition, leading to Sens downregulation, and termination of the neural competence of PWM bristles. The role of ecdysone signaling to repress br after the prepupa-to-pupa transition is distinct from its conventional role in activation, and requires CSN deneddylating activity and multiple cullins, the major substrates of deneddylation. Several CSN subunits physically associate with ecdysone receptors to represses br at the transcriptional level. We propose a model in which nuclear hormone receptors cooperate with the deneddylation machinery to temporally shutdown downstream target gene expression, conferring a spatial restriction on neural competence at the PWM.
Lipoprotein(a) in Vascular Disease, Cancer and Longevity
June Hsieh Wu
Chang Gung Medical Journal , 2011,
Abstract: Lipoprotein(a) [Lp(a)] is a unique lipoprotein with controversial functions. Lp(a) contains apolipoprotein(a) [apo(a)]covalently attached to apolipoprotein B on the low-densitylipoprotein (LDL) particle. The distribution of blood Lp(a)concentrations in several populations have been found to beskewed with Lp(a) being mostly present at low level (0 – 200mg/L). A high Lp(a) concentration (greater than 200 mg/L) inblood increases the risk of various vascular diseases includingchronic heart disease, acute myocardial infarction and cerebralthrombosis. With Lp(a) potentially having such deleteriouseffects, there is a need to ask what are the evolutionary benefit(s) of Lp(a) to humans and other mammals that have it.Lp(a) has been reported to offer a number of benefits such asproviding protection from LDL cholesterol and providing asource of cholesterol in wound tissue. Furthermore, some evidence is emerging that Lp(a) has anti-tumor properties. Other surveys have indicated thatLp(a) is advantageous because it promotes longevity. Lp(a) is only found in humans, oldworld monkeys and hedgehogs. Individuals who do not express Lp(a) do not show any disease symptoms, which indicates that Lp(a) is not essential for human life. It still remainsunclear why mysterious Lp(a) has evolved and is present in humans. (
Rural Tourism and New Township Development: A Study of Jiuzhou, Guizhou Province, China  [PDF]
Lei Wu, Tai-Chee Wong
Current Urban Studies (CUS) , 2017, DOI: 10.4236/cus.2017.54022
Peasantry is symbolic of China’s rural poverty, especially in mountainous provinces such as Guizhou. Recent public campaigns in China to modernize rural regions have much focused on organizing peasants to be urbanized, and engaged in higher value services, one of which is tourism. The paper has used Jiuzhou, a small township in Guizhou to examine its rural urbanization process through land value enhancement and human resource redeployment of local workforce. Jiuzhou’s proximity to the famous tourist site, Huangguoshu, and its easy access to a trans-province highway and its own heritage attractions have been an enabling power to raise its economic status. The study has found a close relationship between the urban-based tourism development and the pace of urbanization, and local tourism development has held back local residents to some extent from migrating out. Growth has generated greater demand for goods and services which in turn has stimulated higher levels of consumption pattern. Nevertheless, many state-run travel agencies were reported to be more dedicated to “image engineering” than market competition, and environmental conservation, the key for eco-tourism, has been somehow overlooked.
Molecular characterization of RA68 related potentially to rice flower development
Xiaohuai Wu,Tai Wang
Chinese Science Bulletin , 2004, DOI: 10.1360/03wc0554
Abstract: One cDNA whose corresponding mRNA was preferentially accumulated in rice flowers was isolated by PCR-mediated RNA subtraction hybridization and RACE strategy. This gene, termed RA68, consists of three exons and two introns. the putative RA68 protein contained 219 amino acid residues with a putative signal peptide and two domains. The N-terminal domain is hydrophilic and rich in proline and threonine embedded in PTPTSYG motifs. The C-terminal domain is hydrophobic. Southern blot and sequence analysis reveal that RA68 exists as one copy in the rice genome and localizes in chromosome 2. Northern hybridization indicates that RA68 is expressed preferentially in buds and flowers, but not in roots and leaves. Furthermore, in situ hybridization indicates that RA68 transcripts are present in pollen mother cells (PMCs), dyad cells, uninucleate pollens and macrospore sacs. Possible functions of RA68 during the flower development are discussed based on its expression and protein structure characteristics.
Catechol-O-methyltransferase and Parkinson's disease.
Tai CH,Wu RM
Acta Medica Okayama , 2002,
Abstract: Parkinson's disease (PD) is one of the main causes of neurological disability in the elderly. Levodopa is the gold standard for treating this disease, but chronic levodopa therapy is complicated by motor fluctuation and dyskinesia. The catechol-O-methyltransferase (COMT) inhibitors represent a new class of antiparkinsonian drugs. When coadministered with levodopa/decarboxylase inhibitor, 2 COMT inhibitors, tolcapone and entacapone have been shown to improve the clinical benefit of levodopa. COMT activity is genetically polymorphic, and individuals with the low activity (COMT(L/L)) genotype have a thermolabile COMT protein; studies suggest that this genotype is less common in Asians than in Caucasians. Differences in COMT activity may determine the individual response to levodopa and result in ethnic differences in PD susceptibility. Our recent study suggests that the COMTL allele can interact with the MAOB gene to increase the occurrence of PD in Taiwanese. In order to understand this new class of antiparkinsonian drugs, we review their basic properties, pharmacology, and clinical efficacy. The frequency distribution of COMT genetic polymorphisms among different populations and its implications in the etiology and drug response is also discussed.
Statement for Chinese Clinical Trial Registration and Publishing System
Tai-Xiang WU
Zhong Xi Yi Jie He Xue Bao , 2006,
Clinical trial registration system and evidence-based medicine
Tai-xiang WU
Zhong Xi Yi Jie He Xue Bao , 2007,
Abstract: ABSTRACT: The authors briefly introduced the management of clinical test for new drug development, clinical trials for drugs prepared in hospital and post-market drugs, and other types of clinical trials. The mechanism of WHO International Clinical Trial Register Platform (WHO ICTRP), Chinese Clinical Trial Register (ChiCTR) and Chinese Clinical Trial Registration and Publishing Collaboration (ChiCTRPC) were also introduced. The authors suggested the trialists to practice the basic philosophy of evidence-based medicine as the rules of their thought and action, and considered that this is the inner guarantee system for the validity of clinical trials.
Statistical Application in Economics  [PDF]
June Luo
Open Journal of Statistics (OJS) , 2012, DOI: 10.4236/ojs.2012.21013
Abstract: Statisticians have recently proposed some methods for ranking the gene variables with outlier expressions. The major attraction of these methods is their ability to select the variables which show systematic decrease or increase in only a subset of samples in the disease group. In order to fully account for the outliers, in this article, we truncate the expression values and propose an alternative method to rank the variables with systematic increase or decrease. The proposed statistic is very simple to implement. Simulations and real data study show that the proposed statistic has a more powerful ability to rank the variables than some methods in literature.
Universal quark-lepton mixing and determination of neutrino masses
Per Osland,Tai Tsun Wu
Physics , 2000,
Abstract: If three right-handed neutrinos are added to the Standard Model, then, for the three known generations, there are six quarks and six leptons. It is then natural to assume that the symmetry considerations that have been applied to the quark matrices are also valid for the lepton mass matrices. Under this assumption, the solar and atmospheric neutrino data can be used to determine the individual neutrino masses. Three minima have been found, using the chi^2 fit, and, from these minima, it is determined that the mass of the lightest neutrino is 1.3\times10^-3 eV, that of the next heavier neutrino is 1.3\times10^-2 eV, while the mass of the heaviest neutrino is 3.4\times10^-2, 5.8\times10^-2 or 9.4\times10^-2 eV.
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