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Search Results: 1 - 10 of 36742 matches for " Jun Kameoka "
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Sorting of Silica Nanocups by Diameter during Fabrication Process
Parag Deotare,Jun Kameoka
Journal of Nanomaterials , 2007, DOI: 10.1155/2007/71259
Abstract: We demonstrated a new technique to sort nanoparticles based on their dimensions. Due to the interactions between charged droplets and a nonlinear electrostatic field, nanoparticles with different dimensions were deposited at different spatial locations on a given target substrate. By using this principle, we have been able to sort nanocups into three groups with mean diameters of 0.31 μm, 0.7 μm, and 1.1 μm and a standard deviation of 20%. This technique improves the nanoparticle fabrication process not only by decreasing the standard deviation of its dimensions but also by increasing its yield since nanoparticles with different mean diameters can be generated at the same time.
A Low Cost and Versatile STED Superresolution Fluorescent Microscope  [PDF]
Daniel McBride, Chin Su, Jun Kameoka, Stanislav Vitha
Modern Instrumentation (MI) , 2013, DOI: 10.4236/mi.2013.23007

A versatile and inexpensive super-resolution fluorescent microscope that functions as easily as a conventional confocal microscope is described. Components of the microscope were designed on a platform which was placed atop a surplus microscope frame. All optical components and equipments used are given. The excitation and depletion beams are extracted from a compact low-cost supercontinuum light source. The focal spot of the depletion beam at the focal plane is studied and imaged by a 100 nm aperture near-field fiber tip. The collinear excitation and depletion beam focused by a 0.9 numerical aperture microscope objective produce a 90 nm lateral super-resolution as verified by imaging 100 nm diameter fluorescent beads.

Forty-Four Pass Fibre Optic Loop for Improving the Sensitivity of Surface Plasmon Resonance Sensors
Chin B Su,Jun Kameoka
Physics , 2007, DOI: 10.1088/0957-0233/19/1/015204
Abstract: A forty-four pass fibre optic surface plasmon resonance sensor that enhances detection sensitivity according to the number of passes is demonstrated for the first time. The technique employs a fibre optic recirculation loop that passes the detection spot forty- four times, thus enhancing sensitivity by a factor of forty-four. Presently, the total number of passes is limited by the onset of lasing action of the recirculation loop. This technique offers a significant sensitivity improvement for various types of plasmon resonance sensors that may be used in chemical and biomolecule detections.
Measurement of Protein 53 Diffusion Coefficient in Live HeLa Cells Using Raster Image Correlation Spectroscopy (RICS)  [PDF]
Sungmin Hong, Ying-Nai Wang, Hirohito Yamaguchi, Harinibytaraya Sreenivasappa, Chao-Kai Chou, Pei-Hsiang Tsou, Mien-Chie Hung, Jun Kameoka
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2010, DOI: 10.4236/jbnb.2010.11004
Abstract: We have applied Raster Image Correlation Spectroscopy (RICS) technique to characterize the dynamics of protein 53 (p53) in living cells before and after the treatment with DNA damaging agents. HeLa cells expressing Green Fluores-cent Protein (GFP) tagged p53 were incubated with and without DNA damaging agents, cisplatin or eptoposide, which are widely used as chemotherapeutic drugs. Then, the diffusion coefficient of GFP-p53 was determined by RICS and it was significantly reduced after the drug treatment while that of the one without drug treatment was not. It is suggested that the drugs induced the interaction of p53 with either other proteins or DNA. Together, our results demonstrated that RICS is able to detect the protein dynamics which may be associated with protein-protein or protein-DNA interactions in living cells and it may be useful for the drug screening.
Log-Normal Distribution of Single Molecule Fluorescence Bursts in Micro/Nano-Fluidic Channels
Lazar L. Kish,Jun Kameoka,Claes G. Granqvist,Laszlo B. Kish
Physics , 2011, DOI: 10.1063/1.3648118
Abstract: The width and shape of photon burst histograms pose significant limitations to the identification of single molecules in micro/nano-fluidic channels, and the nature of these histograms is not fully understood. To reach a deeper understanding, we performed computer simulations based on a Gaussian beam intensity profile with various fluidic channel diameters and assuming (i) a deterministic (noise-free) case, (ii) photon emission/absorption noise, and (iii) photon noise with diffusion. Photon noise in narrow channels yields a Gaussian burst distribution while additional strong diffusion produces skewed histograms. We use the fluctuating residence time picture [Phys. Rev. Lett. 80, 2386-2388 (1998)] and conclude that the skewness of the photon number distribution is caused by the longitudinal diffusive component of the motion of the molecules as they traverse the laser beam. In the case of strong diffusion in narrow channels, this effect leads to a log-normal distribution. We show that the same effect can transform the separate peaks of the photon burst histograms of multiple molecule mixtures into a single log-normal shape.
Optimum Drift Velocity for Single Molecule Fluorescence Bursts in Micro/Nano-Fluidic Channels
Lazar L. Kish,Jun Kameoka,Claes G. Granqvist,Laszlo B. Kish
Physics , 2012, DOI: 10.1063/1.4739471
Abstract: Photonic burst histograms can be used to identify single protein molecules in micro/nano-fluidic channels provided the width of the histogram is narrow. Photonic shot noise and residence time fluctuations, caused by longitudinal diffusion, are the major sources of the histogram width. This Communication is a sequel to an earlier Letter of ours [L. L. Kish et al., Appl. Phys. Lett. 99, 143121 (2011)] and demonstrates that, for a given diffusion coefficient, an increase of the drift velocity enhances the relative shot noise and decreases the relative residence time fluctuations. This leads to an optimum drift velocity which minimizes the histogram width and maximizes the ability to identify single molecules, which is an important result for applications.
Collection of Macaca fascicularis cDNAs derived from bone marrow, kidney, liver, pancreas, spleen, and thymus
Naoki Osada, Makoto Hirata, Reiko Tanuma, Yutaka Suzuki, Sumio Sugano, Keiji Terao, Jun Kusuda, Yosuke Kameoka, Katsuyuki Hashimoto, Ichiro Takahashi
BMC Research Notes , 2009, DOI: 10.1186/1756-0500-2-199
Abstract: We constructed cDNA libraries of Macaca fascicularis, derived from tissues obtained from bone marrow, liver, pancreas, spleen, and thymus of a young male, and kidney of a young female. In total, 5'-end sequences of 56,856 clones were determined. Including the previously established cDNA libraries from brain and testis, we have isolated 112,587 cDNAs of Macaca fascicularis, which correspond to 56% of the curated human reference genes.These sequences were deposited in the public sequence database as well as in-house macaque genome database http://genebank.nibio.go.jp/qfbase/ webcite. These data will become valuable resources for identifying functional parts of the genome of macaque monkeys in future studies.Macaca fascicularis (cynomolgus, crab-eating, or long-tail macaque) is one of the most popular primate species used in biomedical research, and is closely related to Macaca mulatta (rhesus macaque). The draft sequence of the Macaca mulatta genome, which has an evolutionary important position, was published in 2007 [1].Transcriptiome data broadens the application of genome sequences. Compared with several millions of human transcript sequences, macaque transcriptome data has only been analyzed in a limited numbers of studies [2-6]. A complete list of macaque genes will be beneficial for performing genetic studies using macaques in the future. We aim to elucidate all the macaque transcripts that correspond to human genes, which have been widely accepted as reference sequences, such as the RefSeq sequences [7].We have published expressed sequence tag (EST) and full-length sequences, which were obtained from cDNA libraries of brain and testis of Macaca fascicularis, using a variety of research subjects [5,8-13]. Here, we present 5'-EST sequences from six other tissues of Macaca fascicularis. Bone marrow, liver, pancreas, spleen, and thymus from a 4-year-old male Malaysian Macaca fascicularis, and kidney from a 3-year-old female Philippine Macaca fascicularis were harve
Huge mucinous borderline ovarian cystadenoma in a premenarchal girl  [PDF]
Atsushi Horiuchi, Kazuhiro Kameoka, Kouichi Sato, Yuji Yamamoto, Yuji Watanabe
Open Journal of Pediatrics (OJPed) , 2012, DOI: 10.4236/ojped.2012.21014
Abstract: Epithelial ovarian tumors are common in adult women, but rare in children. Especially mucinous ovarian cystadenoma is extremely rare, with only 16 cases in premenarchal girls reported to date. We present a case of 12-year-old premenarchal girl with symptoms of lower abdominal distension. CT showed a large multilocular tumor spreading throughout the entire abdominal cavity from the right upper quadrant to the pelvic cavity. The tumor was brought out little by little with aspiration of the fluid contents with a small incision. The tumor was found to originate from the left ovary, and oophorectomy was performed. The tumor measured 26 × 18 cm and weighed 5860 g. Histological diagnosis was mucinous ovarian cystadenoma with borderline malignancy. No evidence of recurrence had been seen as of 2 years postoperatively. In the review of premenarchal mucinous borderline ovarian cystadenoma, all cases were stage I and underwent salpingo-oophorectomy or oophorectomy. Those patients were alive without recurrence. Al-though mucinous borderline ovarian cystadenoma was characterized as malignancy, premenarchal cases showed favorable outcomes and salpingo-oohprectomy or oophorectomy appears appropriate.
Large-scale analysis of Macaca fascicularis transcripts and inference of genetic divergence between M. fascicularis and M. mulatta
Naoki Osada, Katsuyuki Hashimoto, Yosuke Kameoka, Makoto Hirata, Reiko Tanuma, Yasuhiro Uno, Itsuro Inoue, Munetomo Hida, Yutaka Suzuki, Sumio Sugano, Keiji Terao, Jun Kusuda, Ichiro Takahashi
BMC Genomics , 2008, DOI: 10.1186/1471-2164-9-90
Abstract: We found that 1024 transcripts did not represent any public human cDNA sequence and examined their expression using M. fascicularis oligonucleotide microarrays. Significant expression was detected for 544 (51%) of the unidentified transcripts. Moreover, we identified 226 genes containing exon alterations in the untranslated regions of the macaque transcripts, despite the highly conserved structure of the coding regions. Considering the polymorphism in the common ancestor of cynomolgus and rhesus macaques and the rate of PCR errors, the divergence time between the two species was estimated to be around 0.9 million years ago.Transcript data from Old World monkeys provide a means not only to determine the evolutionary difference between human and non-human primates but also to unveil hidden transcripts in the human genome. Increasing the genomic resources and information of macaque monkeys will greatly contribute to the development of evolutionary biology and biomedical sciences.Genomic resources and information about primates are valuable for evolutionary and biomedical studies to determine how and why phenotypes specific to humans, as well as human diseases, have been formed. Moreover, they are important for extrapolating the results of laboratory experiments to medical research because the physiology of primates is more similar to that of humans as compared with other common experimental animals such as rodents. The cynomolgus macaque (Macaca fascicularis), also known as the long-tailed or crab-eating macaque, is an Old World monkey living in Southeast Asia. It is bred in laboratories worldwide and is one of the most popular primates used for laboratory animal studies, such as those on infectious diseases, immunology, pharmacology, tissue engineering, gene therapy, senescence, and learning [1]. Cynomolgus macaques, rhesus macaques (M. mulatta), and Japanese macaques (M. fuscata) are widely used for experimental studies and are closely related to each other [2-4]. Th
Underlying—Disease Risk for Antispasmodic Premedication in Older Patients Undergoing Investigations of the Gastrointestinal Tract
Noboru Saito, Akiyoshi Seshimo and Shingo Kameoka
Clinical Medicine Insights: Gastroenterology , 2012,
Abstract: Purpose: Improve risk management of patients undergoing investigations of the gastrointestinal (GI) tract, in regard to underlying diseases and choice of premedication. According to a nationwide survey in Japan, 74% of the deaths associated with premedication were patients aged 60 years or over. Methods: Subjects were 418 patients undergoing investigations of the GI tract (367 endoscopy, 51 barium contrast radiography) between October 2001 and January 2004. Age distribution peaked in the 65-69 years group, and 40% of subjects were aged 65 years and over. Using a questionnaire, each subject was interviewed prior to the investigation to determine contraindications for anticholinergic agents or glucagon preparations. To confirm the risk associated with antispasmodic agents in elderly subjects, the group was subdivided into those aged under 65 years and those aged 65 and over, and com- pared. Results: Anticholinergic agents were contraindicated in more than 50% of subjects aged 65 years and over, and glucagon was contraindicated in 11% of subjects aged 65 years and over. The proportion of elderly subjects in whom antispasmodic agents, including anticholinergics and glucagon, were contraindicated was significantly greater than for subjects aged under 65 years. Conclusions: Using a written questionnaire at the time of obtaining informed consent facilitates the identification of under- lying diseases and selection of appropriate premedication.
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