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OALib Journal期刊

ISSN: 2333-9721

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Identification and distribution of Fusarium oxysporum f. sp. Cubense isolates through analysis of vegetative compatibility group in Lampung province, Indonesia
Jumjunidang,Riska,A. Soemargono
Journal of Engineering and Applied Sciences , 2012,
Abstract: The use of Vegetative Compatibility Group (VCG) analysis in this study was to identify the isolates and the distribution of Fusarium oxysporum f. sp. cubense (Foc) as well as the infected banana varieties. Samples of Foc isolates were collected from the diseased banana plants in Lampung province. While isolating and purifying the isolates with single spore techniques and analyzing the VCG were performed in the laboratory. Fifteen testers of VCGs used were from Australia. The results revealed that 30 isolates of the pathogen Foc have been found at all surveyed locations on 10 banana varieties. These varieties were seven varieties of dessert bananas i.e., Seribu (AA), Muli (AA), Ambon Kuning, Ambon Hijau and Cavendish (AAA), Raja Sere and Raja Bulu (AAB) and three varieties of cooking bananas i.e., Nangka (AAB), Jantan (AAB) and Kepok (ABB/BBB). Seventeen of these 30 isolates were VCGs 01213/16 (TR4) found in seven banana varieties. The other isolates comprised five isolates of VCGs 0120/15 (subTR4) in three banana varieties, three isolates of VCGs 01216 (TR4) in two varieties, one isolate of VCG 01213 (TR4) in one variety and four isolates of unknown VCGs in three varieties. Since VCGs 01213/16 (TR4) was the most prevalent, managing and preventing further spread of the disease in Lampung province should be taken into account.
Confirm the status of VCG Fusarium oxysporum f. sp. Cubense in west Sumatera Indonesia
Riska,Jumjunidang,Catur Hermanto
Journal of Engineering and Applied Sciences , 2012,
Abstract: The objective of the experiment was to confirm the status VCGs of Foc in West Sumatera by identifying of Fusarium oxysporum f. sp. cubense isolates with Vegetative Compatibility Group test. A total 47 isolates of Foc were Indonesian Tropical Fruits Research Institute collection that preserved in paper sterile technique. Isolation, isolate purification and VCG analysis were held at Pest and Disease Laboratory of Indonesian Tropical Fruit Research Institute and Department of Primary Industry, Plant Pathology Section, Indooroopili, Australia and started from Juli 2008 until December 2009. The testers of VCGs used were from Australia, namely: VCG 0120, VCG 0120/15, VCG 01215, VCG 0123, VCG 0124, VCG 0124/5, VCG 0125, VCG 0126, VCG 0128, VCG 01211, VCG 01213, VCG 01213/16, VCG 01216, VCG 01219 and VCG 01220. The results showed that A total of 47 isolates of Foc collection from West Sumatra grouped into five VCG (01213/16, 0121, 01219, 01218 and 0120) and seven isolates are unknown VCGs. A total of 13 varieties were attacked by Foc are Raja/AAB, Raja serai/AAB, Buai/AAA, P. panjang/AAB, Barangan/AAA, Ambon kuning/AAA, Ambon hijau/AAA, Rejang/AAw, Jantan/AAB, Kepok/ABB/BBB, Manih/AA, Cavendish/AAA, siem/ABB and Mas kirana./AA total of 28 isolates (59,5%) grouped into VCG 01213/16 (TR4), attacked 11 varieties of bananas (Raja/AAB, Raja serai/AAB, Buai/AAA, Panjang/AAB, Barangan/AAA, Ambon kuning/AAA, Ambon hijau/AAA, Rejang/AAw, Jantan/AAB, Kepok/ABB/BBB, and Mas kirana/AA).
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