oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2019 ( 181 )

2018 ( 479 )

2017 ( 491 )

2016 ( 532 )

Custom range...

Search Results: 1 - 10 of 203269 matches for " Juan Bautista De Sanctis "
All listed articles are free for downloading (OA Articles)
Page 1 /203269
Display every page Item
estatinas afectan la viabilidad de líneas celulares de leucemia y linfoma humanas in vitro
Guerrero,Mery; Di Giulio,Camilo; De Sanctis,Juan Bautista;
Revista de la Facultad de Medicina , 2010,
Abstract: statins have been proposed to induce apoptosis of tumor cells. in order to test this hypothesis, the effect of atorvastatin, fluvastatin, lovastatin, mevastatin, pravastatin, simvastatin on cell viability was assessed by in vitro culture for 48 hr, at concentrations ranging from 1 pm to 100 μm on human cell lines jurkat e6.1, jurkat d1.1 (t cell lymphoma), daudi (b cell lymphoma), u937 (monocitic leukemia) and hl-60 (pro mielomonocitic leukemia) and analyzed the oxidation of (3-(4.5-dimethylthiazol-2-yl)-2.5- diphenyltetrazolium bromide (mtt). lovastatin and mevastatin are the most potent inductors of cell death independently of the cell type (ic 50 between 12 and 50 μm). differences in the ic50 are observed depending on the cell line: atorvastatina (38.1 and 48.6 μm jurkats, 55.3 μm daudi y 100 μm for the others lines), pravastatin (25 μm hl-60, 55.6 y 60.7 μm jurkats and ≥ 100 μm daudi and u937), simvastatin (25.1 μm jurkat d1.1, 50.2 μm jurkat e6.1, 45.2 μm daudi and 51,3 μm hl-60, and > 100 μm u937) and for fluvastatin > 100 μm in all cases. the decrease in cell viability is reverted completely when the cells were incubated with 10 μm mevalonate. it is concluded that lovastatin and mevastatin are the most potent inductors of cell death followed by atorvastatin, pravastatin and simvastatin whose effect depends upon the cell type and fluvastatin does not have any important effects on cell viability on the cell lines studied.
estatinas afectan la viabilidad de líneas celulares de leucemia y linfoma humanas in vitro
Mery Guerrero,Camilo Di Giulio,Juan Bautista De Sanctis
Revista de la Facultad de Medicina , 2010,
Abstract: Se ha propuesto que las estatinas inducen apoptosis sobre células tumorales. Para probar dicha hipótesis, se analizó el efecto de las estatinas atorvastatina, fluvastatina, lovastatina, mevastatina, pravastatina y simvastatina en el rango de concentraciones de 1 pM hasta 100 μM, sobre la viabilidad de las líneas celulares humanas Jurkat E6.1, Jurkat D1.1 (Linfoma T) , Daudi (Linfoma B), U937 (leucemia monocítica) y HL-60 (leucemia promielomonocítica) in vitro en cultivos de 48 horas, analizados por la técnica de hidrolización del compuesto bromuro de 3-(4,5-dimetiltiazol-2-il)-2,5-difenilltetrazolio (MTT). Lovastatina y mevastatina son los más potentes inductores de muerte celular independientemente del tipo celular (Ic 50 entre 12 y 50 μM). Para las otras estatinas se observan diferencias en el Ic50 según la línea celular atorvastatina (38,1 y 48,6 μM Jurkats, 55,3 μM Daudi y 100 μM para las otras líneas), pravastatina (25 μM HL-60, 55,6 y 60,7 μM Jurkats y ≥ 100 μM Daudi y U937), simvastatina (25,1 μM Jurkat D1.1, 50,2 μM Jurkat E6.1, 45,2 μM Daudi y 51,3 μM HL-60, y > 100 μM U937) y para fluvastatina en todos los casos > 100 μM. La disminución de la viabilidad celular se revierte completamente cuando las células son incubadas con 10 μM mevalonato. Se concluye que la lovastatina y mevastatina son las más potentes inductoras de muerte seguida por atorvastatina, pravastatina y simvastatina cuyo efecto depende del tipo de línea celular y la fluvastatina no tiene efectos importantes en la viabilidad de las líneas celulares estudiadas. Statins have been proposed to induce apoptosis of tumor cells. In order to test this hypothesis, the effect of atorvastatin, fluvastatin, lovastatin, mevastatin, pravastatin, simvastatin on cell viability was assessed by in vitro culture for 48 hr, at concentrations ranging from 1 pM to 100 μM on human cell lines Jurkat E6.1, Jurkat D1.1 (T cell lymphoma), Daudi (B cell lymphoma), U937 (monocitic leukemia) and HL-60 (pro mielomonocitic leukemia) and analyzed the oxidation of (3-(4.5-Dimethylthiazol-2-yl)-2.5- diphenyltetrazolium bromide (MTT). Lovastatin and mevastatin are the most potent inductors of cell death independently of the cell type (Ic 50 between 12 and 50 μM). Differences in the Ic50 are observed depending on the cell line: atorvastatina (38.1 and 48.6 μM Jurkats, 55.3 μM Daudi y 100 μM for the others lines), pravastatin (25 μM HL-60, 55.6 y 60.7 μM Jurkats and ≥ 100 μM Daudi and U937), simvastatin (25.1 μM Jurkat D1.1, 50.2 μM Jurkat E6.1, 45.2 μM Daudi and 51,3 μM HL-60, and > 100 μM U937) and for fluvastatin >
Niveles séricos de metaloproteinasa 9 (mmp-9) y del inhibidor tisular de mmp tipo 1 (timp-1) en pacientes venezolanos con asma o con enfermedad pulmonar obstructiva crónica (epoc)
Larocca,Nancy Elizabeth; Moreno,Dolores; Garmendia,Jenny; De Sanctis,Juan Bautista;
Revista de la Facultad de Medicina , 2010,
Abstract: serum levels of metalloproteinase (mmp) 9, an enzyme involved in inflammation, and its counterpart, the tissue inhibitor of metalloproteinases type 1 (timp-1) are significantly (p < 0.01) increased in patients with asthma or with chronic obstructive pulmonary disease (copd) as compared with controls of the same age. the increase in both parameters is more significant (p < 0.0001) in severe patients of both groups, and particularly in patients with severe copd as compared to severe asthmatics (p < 0.01). the increase in serum mmp-9 levels in relation with severity is higher than the observed values for timp-1 in both diseases. it is concluded that serum levels of mmp-9 and timp-1 may be important markers to establish severity in these diseases.
Polimorfismos de receptores semejantes a Toll 2 y 4 en pacientes con asma bronquial y enfermedad pulmonar obstructiva crónica (EPOC)
Larocca G,Nancy Elizabeth; De Sanctis,Juan Bautista; Toro,Félix; Moreno,Dolores; Tálamo,Carlos;
Revista de la Facultad de Medicina , 2008,
Abstract: toll like receptors are important in the inflammatory immune response. several mutations of these receptors have been related to the development and chronicity of different inflammatory diseases as bronchial asthma and obstructive pulmonary disease. a case control study was performed, 100 patients with bronchial asthma, 100 obstructive pulmonary disease and 100 controls. restriction based polymorphism pcr analysis was performed for tlr2 (arg677trp and arg753gln) and tlr4 (asp299gly and thr399ile) genes and its association with asthma and obstructive pulmonary disease was assessed. the frequency of tlr4 polymorphism was 9 % in each group studied (asthma, obstructive pulmonary disease and controls). no association was found among tlr4 polymorphism and stage of disease for asthma or obstructive pulmonary disease. tlr4 polymorphism are not associated with the presence or severity of asthma or obstructive pulmonary disease in the venezuelan population.
HLA y enfermedad pulmonar obstructiva crónica (EPOC)
Canónico S,Yeilis C; Larocca G,Nancy Elizabeth; Moreno,Dolores; De Sanctis,Juan Bautista;
Revista de la Facultad de Medicina , 2008,
Abstract: obstructive pulmonary disease is characterized by the progressive obstruction of air flow and it is partially reversible due to the destruction of the pulmonary parenchyma and inflammation of the air ways. the development of this inflammatory pathology depends on several factors including the major histocompatibility complex. few studies have dealt with the possible role of major histocompatibility complex variations and obstructive pulmonary disease development and chronicity. major histocompatibility complex class i and ii genetic analysis was performed in 50 hybrid patients, with technique of amplification of specific primers (pcr-ssp) (genovision?). as a comparison 108 controls were used. no significant differences were encountered in frequencies of class i and ii patients with obstructive pulmonary disease and controls. no association was found in major histocompatibility complex polymorphism and obstructive pulmonary disease. further studies should assess other genes for ascertain susceptibility to develop the disease.
Polimorfismos de receptores semejantes a Toll 2 y 4 en pacientes con asma bronquial y enfermedad pulmonar obstructiva crónica (EPOC)
Nancy Elizabeth Larocca G,Juan Bautista De Sanctis,Félix Toro,Dolores Moreno
Revista de la Facultad de Medicina , 2008,
Abstract: Los receptores semejantes a Toll forman parte del sistema inmune. Algunas mutaciones en estos receptores se han relacionado con el desarrollo de enfermedades como el asma bronquial y la enfermedad pulmonar obstructiva crónica. Se realizó un estudio de casos-controles, en el que se incluyeron 100 pacientes con asma bronquial, 100 pacientes con enfermedad pulmonar obstructiva crónica y 100 controles. A través de la técnica de reacción en cadena de la polimerasa, se investigó la presencia de polimorfismos en TLR2 (Arg677Trp y Arg753Gln) y TLR4 (Asp299Gly y Thr399Ile) y su asociación con asma y enfermedad pulmonar obstructiva crónica. Se demostró la presencia de polimorfismos en TLR4 con una frecuencia del 9 % en cada grupo de estudio. No se observó relación alguna entre la presencia de polimorfismos en TLR4 y los índices de gravedad para asma bronquial o enfermedad pulmonar obstructiva crónica. La gravedad de los pacientes con asma y enfermedad pulmonar obstructiva crónica no está relacionada con los polimorfismos en TLR 4. Toll like receptors are important in the inflammatory immune response. Several mutations of these receptors have been related to the development and chronicity of different inflammatory diseases as bronchial asthma and obstructive pulmonary disease. A case control study was performed, 100 patients with bronchial asthma, 100 obstructive pulmonary disease and 100 controls. Restriction based polymorphism PCR analysis was performed for TLR2 (Arg677Trp and Arg753Gln) and TLR4 (Asp299Gly and Thr399Ile) genes and its association with asthma and obstructive pulmonary disease was assessed. The frequency of TLR4 polymorphism was 9 % in each group studied (asthma, obstructive pulmonary disease and controls). No association was found among TLR4 polymorphism and stage of disease for asthma or obstructive pulmonary disease. TLR4 polymorphism are not associated with the presence or severity of asthma or obstructive pulmonary disease in the Venezuelan population.
HLA y enfermedad pulmonar obstructiva crónica (EPOC)
Yeilis C Canónico S,Nancy Elizabeth Larocca G,Dolores Moreno,Juan Bautista De Sanctis
Revista de la Facultad de Medicina , 2008,
Abstract: La enfermedad pulmonar obstructiva crónica se caracteriza por una obstrucción al flujo aéreo a consecuencia de un proceso inflamatorio crónico que depende de muchos factores, entre ellos, el control a nivel genético. La presencia de polimorfismos en el sistema de antígenos leucocitarios humanos ha sido asociado con varias enfermedades. Pocos estudios se han enfocado hacia el posible rol de las variaciones en el locus antígenos leucocitarios humanos humano y el desarrollo de la enfermedad pulmonar obstructiva crónica. Se realizó la tipificación de antígenos leucocitarios humanos clase I y II en 50 pacientes de raza mestiza, con diagnóstico de enfermedad pulmonar obstructiva crónica, utilizando la técnica de PCR-SSP, comparando los pacientes estudiados con 108 controles. No se encontró diferencia estadísticamente significativa entre pacientes con enfermedad pulmonar obstructiva crónica y controles, con respecto a la frecuencia de los alelos antígenos leucocitarios humanos clase I y II. No se encontró asociación de alelos del sistema antígenos leucocitarios humanos con presencia de enfermedad pulmonar obstructiva crónica. Obstructive pulmonary disease is characterized by the progressive obstruction of air flow and it is partially reversible due to the destruction of the pulmonary parenchyma and inflammation of the air ways. The development of this inflammatory pathology depends on several factors including the major histocompatibility complex. Few studies have dealt with the possible role of major histocompatibility complex variations and obstructive pulmonary disease development and chronicity. Major histocompatibility complex class I and II genetic analysis was performed in 50 hybrid patients, with technique of amplification of specific primers (PCR-SSP) (Genovision ). As a comparison 108 controls were used. No significant differences were encountered in frequencies of class I and II patients with obstructive pulmonary disease and controls. No association was found in major histocompatibility complex polymorphism and obstructive pulmonary disease. Further studies should assess other genes for ascertain susceptibility to develop the disease.
Niveles séricos de metaloproteinasa 9 (mmp-9) y del inhibidor tisular de mmp tipo 1 (timp-1) en pacientes venezolanos con asma o con enfermedad pulmonar obstructiva crónica (epoc)
Nancy Elizabeth Larocca,Dolores Moreno,Jenny Garmendia,Juan Bautista De Sanctis
Revista de la Facultad de Medicina , 2010,
Abstract: Los niveles séricos de la metaloproteinasa (MMP) 9, enzima involucrada en inflamación, y su contraparte, el inhibidor tisular de la metaloproteinasas tipo 1 (TIMP-1), se encuentran significativamente (P < 0,01) incrementados en pacientes con asma o con enfermedad pulmonar obstructiva crónica (EPOC) en comparación con los grupos control del mismo rango etario. El incremento de ambos parámetros se hace más significativo en los pacientes severos de ambos grupos (P < 0,0001) y particularmente en los pacientes con EPOC severo en comparación con los asmáticos severos (P < 0,01). El incremento en los niveles séricos de MMP-9 en relación con severidad es mayor que lo observado para TIMP-1 en ambas patologías. Se concluye que los niveles séricos de MMP-9 y TIMP-1 pueden ser un marcador importante para determinar severidad en estas enfermedades. Serum levels of metalloproteinase (MMP) 9, an enzyme involved in inflammation, and its counterpart, the tissue inhibitor of metalloproteinases type 1 (TIMP-1) are significantly (P < 0.01) increased in patients with asthma or with chronic obstructive pulmonary disease (COPD) as compared with controls of the same age. The increase in both parameters is more significant (P < 0.0001) in severe patients of both groups, and particularly in patients with severe COPD as compared to severe asthmatics (P < 0.01). The increase in serum MMP-9 levels in relation with severity is higher than the observed values for TIMP-1 in both diseases. It is concluded that serum levels of MMP-9 and TIMP-1 may be important markers to establish severity in these diseases.
Expresión de los receptores para el Fc de la IgG en leucocitos de sangre periférica de pacientes con hepatitis C crónica Expression of Fc receptors for IgG in peripheral blood leucocytes from hepatitis C virus infected individuals
María Peinado,Angela Conesa,Luisana Dávila,Juan Bautista De Sanctis
Investigación Clínica , 2007,
Abstract: La respuesta inmunitaria representa un elemento fundamental en el control de la infección por el Virus de Hepatitis C (VHC). Factores de origen viral y del hospedero modulan dicha respuesta. El presente trabajo tuvo como objetivo caracterizar complejos inmunitarios, bajo la forma de crioglobulinas, de pacientes infectados con el VHC y evaluar la expresión de superficie de los receptores para el Fc de la IgG (FcγR) en subpoblaciones leucocitarias de sangre periférica de estos pacientes. Se seleccionaron 12 individuos VHC (+) y 12 sujetos sanos. De ambos grupos se tomaron muestras de suero para aislar crioglobulinas y sangre venosa con EDTA para evaluar la expresión de los FcγR CD64 (FcγRI), CD32 (FcγRII) y CD16 (FcγRIII) mediante citometría de flujo. La presencia de ARN del VHC en suero y crioglobulinas fue analizada mediante RT-PCR. Los resultados muestran que el 50% de los pacientes VHC (+) presentaron niveles elevados de crioglobulinas constituidas fundamentalmente por IgG. En 3 de 5 pacientes con crioglobulinas elevadas se identificó ARN del VHC. La expresión de CD64 se observó principalmente en monocitos (80%), CD32 en monocitos, linfocitos B y neutrófilos (> 90%) y CD16 en células NK y neutrófilos (85% y 95% respectivamente) no encontrándose diferencias significativas entre pacientes y controles. La densidad de expresión de CD32 resultó significativamente menor en poblaciones de monocitos y neutrófilos de pacientes en comparación con controles (p < 0,05). Se concluye que esta disminución en la densidad del receptor FcγRII podría tener implicaciones en la fisiopatología de la infección por el VHC. The immune response represents a fundamental element in the control of Hepatitis C virus (HCV) infection. Viral and cellular host factors may modulate this response. In the present study, we characterized immune complexes (cryoprecipitates) isolated form HCV-infected patients and evaluated the expression of Fc receptors for IgG (FcγR) in peripheral blood leucocytes of these patients. Twelve HCV (+) patients and 12 healthy control individuals were selected for this study. For each group, sera samples were collected for cryoglobulins isolation and characterization and EDTA-anticoagulated venous blood samples were collected for flow cytometry analysis of FcγR, CD64 (FcγRI), CD32 (FcγRII) and CD16 (FcγRIII) expression. Presence of HCV RNA in serum and cryoprecipitates was analysed by RT-PCR. Results show that 50% of HCV-infected patients present high levels of cryoglobulins mainly constituted by IgG. Three out of 5 cryoglobulins analyzed by RT-PCR were positiv
Expresión de los receptores para el Fc de la IgG en leucocitos de sangre periférica de pacientes con hepatitis C crónica
Peinado,María; Conesa,Angela; Dávila,Luisana; De Sanctis,Juan Bautista; Deibis,Leopoldo; Toro,Félix;
Investigación Clínica , 2007,
Abstract: the immune response represents a fundamental element in the control of hepatitis c virus (hcv) infection. viral and cellular host factors may modulate this response. in the present study, we characterized immune complexes (cryoprecipitates) isolated form hcv-infected patients and evaluated the expression of fc receptors for igg (fcγr) in peripheral blood leucocytes of these patients. twelve hcv (+) patients and 12 healthy control individuals were selected for this study. for each group, sera samples were collected for cryoglobulins isolation and characterization and edta-anticoagulated venous blood samples were collected for flow cytometry analysis of fcγr, cd64 (fcγri), cd32 (fcγrii) and cd16 (fcγriii) expression. presence of hcv rna in serum and cryoprecipitates was analysed by rt-pcr. results show that 50% of hcv-infected patients present high levels of cryoglobulins mainly constituted by igg. three out of 5 cryoglobulins analyzed by rt-pcr were positive for hcv-rna. expression of cd64 was observed mainly in monocytes (80%), cd32 in monocytes, b lymphocytes and neutrophils (> 90%) and cd16 in nk cells and neutrophils (85% and 95% respectively). no differences were observed in the percentage of fcγr expression when comparing hcv-infected patients with healthy controls. on the contrary, density of expression of cd32 in monocytes and neutrophils cell populations of hcv patients was significantly lower than that observed in healthy controls (p < 0.05). we concluded that low density expression of fcγrii in hcv-infected patients may have implications in the physiopatholgy of hcv infection.
Page 1 /203269
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.