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Search Results: 1 - 10 of 287643 matches for " José Maria Paz de; Silva "
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Study of carbohydrates present in the cladodes of Opuntia ficus-indica (fodder palm), according to age and season
Ribeiro, Erika Maria de Oliveira;Silva, Nicácio Henrique da;Lima Filho, José Luiz de;Brito, Júlio Zoe de;Silva, Maria da Paz Carvalho da;
Ciência e Tecnologia de Alimentos , 2010, DOI: 10.1590/S0101-20612010000400015
Abstract: types and content of carbohydrates were evaluated by chemical (spectrophotometric assay) and physicochemical (thin layer chromatography - tlc and high performance liquid chromatography - hplc) methods in some opuntia ficus-indica varieties according to age and season. the samples comprised four varieties of palm (giant, copena f1, clone 20, and round palm). the results demonstrated that the four varieties of palm contain a good quantity of neutral and acid sugars in both summer and winter seasons. however, samples collected in the summer presented a higher content of carbohydrates specially glucose, fructose, galactose, xylose, and arabinose. the tertiary cladode (old cladode) presented almost the double content of sugar found in the quaternary cladodes (young cladodes). pectic polysaccharides were sequentially extracted with water at 60 oc, and edta at 60 oc solution resulting in water-soluble pectin (wsp) and a chelating-soluble pectin (csp) respectively. galacturonic acid was detected in the fractions wsp and csp. however, the fraction csp presented the highest content of sugar acid.
In Vitro” Antibacterial Activity of the Hidroalcoholic Extract of the Schinus terebinthifolius Raddi Barks  [PDF]
Erica Renata Nogueira Sá, Cássio Ilan Soares Medeiros, Juliana Raquel de Morais Santos Oliveira, Antonio José de Jesus Evangelista, Maria Iranilda Silva Magalh?es, Geraldo Gon?alves de Almeida Filho, Andréa Maria Rolim da Paz, Hilzeth de Luna Freire Pess?a
Advances in Microbiology (AiM) , 2014, DOI: 10.4236/aim.2014.412083
Abstract: The Schinus terebinthifolius Raddi is a native plant of South America popularly known in Brazil as aroeira. It is a medium-sized plant, which demonstrates a high adaptive potential in various environments, besides having various medicinal properties such as anti-inflammatory and antidiarrheal. Bacterial susceptibility tests were carried out and the minimal inhibitory concentration (MIC) was obtained. The results were interpreted based on the conventionally microbiological protocols and data from the CLSI. For the microbiological tests, microorganisms obtained from the American Type Culture Collection (ATCC), specifically, (Pseudomonas aeruginosa, Staphylococcus aureus and Encherichia coli) were used. Of the tested bacterial strains, only the Staphylococcus aureus 6538 presented susceptibility to the aroeira’s hydroalcoholic extract, forming zones of inhibition of 8.0 mm of diameter up to the MIC of 35.3 mg/mL, while the other tested strains showed to be resistant in all the concentrations of hydroalcoholic extract of the Schinus terebinthifolius Raddi bark. Our aim is to analyze the “in vitro” antibacterial potential of the 70% hydroalcoholic extract of the Schinus terebinthifolius Raddi.
Primeiro registro de Aedes (Stegomyia) albopictus no Estado do Ceará, Brasil
Martins,Víctor Emanuel Pessoa; Martins,Marlos Gomes; Araújo,José Maria Paz de; Silva,Luís Osvaldo Rodrigues; Monteiro,Hamilton Antonio de Oliveira; Castro,Francisco Corrêa; Vasconcelos,Pedro Fernando da Costa; Guedes,Maria Izabel Florindo;
Revista de Saúde Pública , 2006, DOI: 10.1590/S0034-89102006000500027
Abstract: for the first time, the occurrence of aedes (stegomyia) albopictus in an urban area of the city of fortaleza, northeastern, brazil, is reported. from january to july 2005, ovitraps were used to collect eggs from aedes spp., which were kept under laboratory conditions to develop into the adult phase. the resultant mosquitoes were identified and subjected to dengue virus isolation tests. thirteen specimens of aedes albopictus, all females, were identified. no dengue virus was isolated in any of the mosquito pools. even though aedes albopictus has not been incriminated in brazilian dengue outbreaks, the possibility of dengue virus transmission by these mosquitoes cannot be dismissed.
Influência do fósforo e de diferentes regimes de corte na produtividade e no perfilhamento do capim-de-raiz (Chloris orthonoton Doell)
Oliveira, Tatiana Neres de;Paz, Luiz Gonzaga da;Santos, Mércia Virginia Ferreira dos;Dubeux Júnior, José Carlos Batista;Ferreira, Rinaldo Luiz Caraciolo;Pires, Aureliano José Vieira;Silva, Maria da Concei??o;
Revista Brasileira de Zootecnia , 2004, DOI: 10.1590/S1516-35982004000100009
Abstract: the work was carried out in a greenhouse, at the animal science department/ufrpe, from october 2000 to may 2001, to evaluate the effect of phosphorus fertilization and different cut systems on the productivity and tillering of the capim-de-raiz (chloris orthonoton, doell). the experimental design was completely randomized, distributed in a factorial arrangement 3 x 2 x 2, being three levels of application of phosphorus fertilizer (0, 100 and 200 kg/ha of p2o5), two cut frequencies (30 and 40 days) and two cut intensities (5 and 15 cm). significant interaction between cut frequency and cut intensity as well as phosphorus fertilization and cut frequency for the dm yield was observed. the best combination between cut frequency and cut intensity was 40 days and 5 cm, respectively, with 10.66 g/pot of dm. the phosphorus fertilization showed higher efficacy when the cut frequency was 40 days (11.70 g/pot of dm). phosphorus fertilization, cut frequency and cut intensity showed significant effect on the tillering process.
Polineuropatia periférica em pacientes com lúpus eritematoso sistêmico juvenil
Almeida, Roberta Tavares;Campos, Lúcia Maria de Arruda;Aikawa, Nádia Emi;Kozue, Kátia Tomie;Paz, José Albino da;Marques-Dias, Maria Joaquina;Silva, Clovis Artur Almeida;
Revista Brasileira de Reumatologia , 2009, DOI: 10.1590/S0482-50042009000400004
Abstract: introduction: peripheral polyneuropathy is one of 19 neuropsychiatric syndromes seen in systemic lupus erythematosus, according to the classification criteria proposed by the american college of rheumatology (acr) for neuropsychiatric syndromes. however, this manifestation has not been reported very often, especially in patients with juvenile systemic lupus erythematosus (jsle). patients and methods: from 1983 to 2007, 5,079 patients were seen at the pediatric rheumatology unit of the icr-hc-fmusp; 228 (4.5%) patients were diagnosed with jsle according to the criteria of the acr. peripheral polyneuropathy was diagnosed according to the criteria for neuropsychiatric syndromes of the acr. results: five (2.2%) out of 228 patients with jsle developed peripheral polyneuropathy and were described retrospectively. the diagnosis was confirmed by electroneuromyography, which showed the presence of distal peripheral polyneuropathy, sensorial and/or motor, involving all four limbs, in two patients, and the lower limbs, in three patients. three of those patients were females, and peripheral neuropathy developed after the diagnosis of jsle. the mean age of onset of the disease was 14 years, and the mean time between the onset of jsle and the diagnosis of peripheral polyneuropathy was 23 months. the most common clinical presentations included muscular weakness and hyporeflexia. antiphospholipid antibodies were present in all patients. treatment consisted of corticosteroids in all patients, associated with intravenous cyclophosphamide in three patients. one patient evolved to functional disability and paresis of the lower limbs, requiring a wheelchair. one female patient died of severe sepsis. conclusions: peripheral polyneuropathy is a rare, severe, and occasionally incapacitating manifestation of jsle, commonly associated with the presence of antiphospholipid antibodies.
HLA-B*44 Is Associated with Dengue Severity Caused by DENV-3 in a Brazilian Population
Liciana Xavier Eurico de Alencar,Ulisses de Mendon?a Braga-Neto,Eduardo José Moura do Nascimento,Marli Tenório Cordeiro,Ana Maria Silva,Carlos Alexandre Antunes de Brito,Maria da Paz Carvalho da Silva,Laura Helena Vega Gonzales Gil,Silvia Maria Lucena Montenegro,Ernesto Torres de Azevedo Marques Júnior
Journal of Tropical Medicine , 2013, DOI: 10.1155/2013/648475
Abstract: Human leukocyte antigen (HLA) alleles have been correlated with susceptibility or resistance to severe dengue; however, few immunogenetic studies have been performed in Latin American (LA) populations. We have conducted immunogenetic studies of HLA class I and II alleles in a cohort of 187 patients with DENV-3 infection and confirmed clinical diagnosis of either severe dengue, known as dengue hemorrhagic fever (DHF), or the less severe form, dengue fever (DF), in Recife, Pernambuco, Brazil. An association analysis was performed using Fisher’s association test, with odds ratios (ORs) calculated using conditional maximum likelihood estimates. HLA-B*44 ( , OR = 2.025, 95% CI = 0.97–4.24) was found to be associated with increased susceptibility to DHF in response to DENV-3 infection. In addition, HLA-B*07 ( , OR = 0.501, one-sided 95% CI = 0–0.99) and HLA-DR*13 ( , OR = 0.511, one-sided 95% CI = 0–0.91) were found to be associated with resistance to secondary dengue infection by DENV-3. These results suggest that HLA-B*44 supertype alleles and their respective T-cell responses might be involved in susceptibility to severe dengue infections, whereas the HLA-B*07 supertype alleles and DR*13 might be involved in cross-dengue serotype immunity. 1. Introduction Dengue virus (DENV) has four serotypes, named as DENV-1, DENV-2, DENV-3 and DENV-4. The majority of dengue infections are subclinical; however, the clinical manifestations of dengue infection range from the benign, self-limited dengue fever (DF) to a vasculopathy syndrome known as dengue hemorrhagic fever (DHF) that can lead to hypovolemic dengue shock syndrome (DSS). It is often observed in epidemiological studies that the more severe illness occurs more frequently in secondary heterotypic dengue infection [1–7]. There are two main hypotheses to explain the higher frequency of DHF in secondary infections. The first is that heterotypic antibodies would bind to the dengue virus and would facilitate the viral entry into cells expressing Fc receptors; this theory is known as antibody dependent enhancement (ADE) [8, 9]. The second hypothesis is that anti-dengue memory T cells that cross-react with related but altered peptide epitopes would induce the T cells to produce abnormal levels of cytokines leading to vasculopathy. This theory is known as “original antigenic sin” (OAS) or “altered peptide ligand” (APL). The theories are not mutually exclusive, and both are based on the fact that previous dengue infection is a risk factor for developing more severe disease. However, 99% or more of the secondary dengue
Screening Test for Antibiotics in Medicinal Plants (STAMP): Using Powdered Plant Materials Instead of Extracts  [PDF]
Marielle Cascaes Inácio, Fabio Carmona, Tiago Antunes Paz, Maysa Furlan, Fernando Arcanjo da Silva, Bianca Waléria Bertoni, Suzelei de Castro Fran?a, Ana Maria Soares Pereira
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.412290

Plants are a rich source of antibiotics, but screening all the existing plant species for biological activity using current methods can be time and resource consuming. The present study is to investigate whether powdered plant materials would perform as well as plant extracts in the screening of plants with antimicrobial activity. In the new method proposed (STAMP), we compared in vitro antimicrobial activity of powdered plant materials from 12 species against bacteria and fungi. We confirmed these results with their corresponding aqueous (wet) and hydro-alcoholic extracts and one species testing the antimicrobial activity of two isolated compounds. Compared with hydro-alcoholic extracts, screening using the powdered plant materials correctly identified the majority of the species with antimicrobial activity against Candida albicans (sensitivity 91%, specificity 86%), C. parapsilosis (sensitivity 100%, specificity 67%), and Staphylococcus aureus (sensitivity 64%, specificity 86%). For bacteria, the method performed better in a pH of 9.0. The antimicrobial activity of two compounds isolated from one species (maytenin and netzahualcoyone) confirmed the results. In conclusion, the use of powdered plant materials for screening plants with antimicrobial properties is a cheap, widely available, technically easy, time sparing, reproducible, and sensitive method and can significantly shorten the time and money spent during drug development.

An empiric equation for the latent heat of vaporization of moisture in bananas during its isothermal drying  [PDF]
Wilton Pereira da Silva, Cleide Maria Diniz Pereira da Silva e Silva, Fernando José de Almeida Gama, Josivanda Palmeira Gomes
Agricultural Sciences (AS) , 2012, DOI: 10.4236/as.2012.32025
Abstract: In order to determine the energy needed to artificially dry a product, the latent heat of vaporization of moisture in the product, hfg, must be known. Generally, the expressions for hfg reported in the literature are of the form hfg = h(T)f(M), where h(T) is the latent heat of vaporization of free water, and f(M) is a function of the equilibrium moisture content, M. But expressions of this type contain a simplification because, in this case, the ratio hfg/h would only depend to the moisture content. In this article a more general expression for the latent heat of vaporization, namely hfg = g(M,T), is used to determine hfg for banana. To this end, a computer program was developed which fits automatically about 500 functions, with one or two independent variables, imbedded in its library to experimental data. The program uses nonlinear regression, and classifies the best functions according to the least reduced chi-square. A set of executed statistical tests shows that the generalized expression used in this work given by hfg = g(M,T) produces better results of hfg for bananas than other equations found in the literature.
A realiza??o do teste anti-hiv no pré-natal: os significados para a gestante
Silva, Roberta Maria de Oliveira;Araújo, Carla Luzia Fran?a;Paz, Fatima Maria Trigo da;
Escola Anna Nery , 2008, DOI: 10.1590/S1414-81452008000400004
Abstract: the study had as objective, the knowledge and the analyzis of the meaning of the accomplishment for the test anti-hiv in the prenatal one for the pregnant women. one is about a research with qualitative approach and was carried through in a hospital school and a maternity at the city of rio de janeiro. as a resource of technician-methodological, the speech of the collective citizen was used (dsc). after the analysis of the speeches we verified that, for the pregnant women the accomplishment of the test means the possibility to prevent the vertical transmission of the hiv and as part of the prenatal assistance. the prenatal one was considered by the pregnant women an excellent chance for the accomplishment of the test anti hiv, for the knowledge of the serological condition and precocious beginning of the treatment. it is concluded that the test, for the majority of the pregnant women, represents the possibility to protect the son of the hiv, beyond being part of the construction of the maternal paper from a well-taken care of concrete with the health of the baby.
Eco-epidemiological aspects of Trypanosoma cruzi, Trypanosoma rangeli and their vector (Rhodnius pallescens) in Panama
Vasquez, Ana Maria de;Samudio, Franklyn E.;Salda?a, Azael;Paz, Hector M.;Calzada, José E.;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2004, DOI: 10.1590/S0036-46652004000400008
Abstract: the eco-epidemiology of t. cruzi infection was investigated in the eastern border of the panama canal in central panama. between 1999 and 2000, 1110 triatomines were collected: 1050 triatomines (94.6%) from palm trees, 27 (2.4%) from periurban habitats and 33 (3.0%) inside houses. all specimens were identified as r. pallescens. there was no evidence of vector domiciliation. salivary glands from 380 r. pallescens revealed a trypanosome natural infection rate of 7.6%, while rectal ampoule content from 373 triatomines was 45%. isoenzyme profiles on isolated trypanosomes demonstrated that 85.4% (n = 88) were t. cruzi and 14.6% (n = 15) were t. rangeli. blood meal analysis from 829 r. pallescens demonstrated a zoophilic vector behavior, with opossums as the preferential blood source. seroprevalence in human samples from both study sites was less than 2%. our results demonstrate that t. cruzi survives in the area in balanced association with r. pallescens, and with several different species of mammals in their natural niches. however, the area is an imminent risk of infection for its population, consequently it is important to implement a community educational program regarding disease knowledge and control measures.
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