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Search Results: 1 - 10 of 108024 matches for " José Eduardo Brasil Pereira Pinto "
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Enraizamento in vitro de microestacas e micropropaga??o de gemas axilares de Sarandi (Sebastiania schottiana Muell. arg.)
Deschamps, Cícero;Pinto, José Eduardo Brasil Pereira;
Ciência Rural , 1995, DOI: 10.1590/S0103-84781995000300010
Abstract: in vitro rooting was carried out on murashige & skoog (ms) and wood plant medium (wpm) liquid and solid, suppiemented with indolbutiric acid (iba). multipleshoot induction was tested with benzylaminopurine (bap) on ms and wpm medium, with one explant in each tube. the explant was inoculated in container (200ml) on ms medium suppiemented with bap and naa. the last experiment was made with bap, ga3 and activaded charcoal. in the present work, the results show better rooting percentage and number of roots on wpm medium with 4.9μm iba. the multiplication of shoots was obtained with ga3, at 1.4μm that resulted in better lenght of shoots and number of buds. bap was effective for all characteristics at 1.1μm only without actived charcoal.
Resposta à regenera??o e crescimento de brotos in vitro de Kielmeyera coriacea quando influenciado por diferentes concentra??es dos sais e de sacarose
Pinto, José Eduardo Brasil Pereira;Arello, Eduardo Fonseca;Pinto, César Augusto Brasil Pereira;Barbosa, Márcio Henrique Pereira;
Ciência Rural , 1996, DOI: 10.1590/S0103-84781996000100011
Abstract: trials were carried out to test sucrose and salt concentrations added to growth medium on regeneration and growth response of kielmeyera coriacea shoots. the reduction and increase of sucrose of 30 and 45g/l, rerspectively, caused a decrease in total number of shoots. a higher shoots percentage of with more than 1.0cm, occurred on media with 30g/l of sucrose. the use of total force (1/1) and half strength (1/2) of ms salt proportioned a higher shoot ratio and growth.
Optimization of the Protocol for the in Vitro Cultivation of Piper aduncum L.  [PDF]
Samia Torres Silva, Fernanda Ventorim Pacheco, Ivan Caldeira Almeida Alvarenga, José Eduardo Brasil Pereira Pinto, Suzan Kelly Vilela Bertolucci, Cibelly Pereira Ferreira
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.523363
Piper aduncum L. (Piperaceae) has great potential for economic exploration because of the proven use of its essential oil in the agriculture and in the human health. A technique that improves its propagation, as the tissue culture, becomes necessary. Some parameters must be determined for the successful cultivation in vitro. Thus, this study aimed to determine the salts concentration of MS medium, temperature, luminosity and light quality for in vitro culture of this species. The following treatments were conducted: 1/4MS, 1/2MS, MS and 2MS; 20, 25, 30 and 35; monochromatic blue, red and white lights and the combination of red and blue, using light emitting diodes (LEDs); luminosities of 17, 37, 48 and 73 μmol·m-2·s-1 obtained with cool white fluorescent lamp. After 35 days, the treatments were analyzed. To determine the salts concentration of MS, the variables were evaluated: number of shoots, leaves and roots, length and width of leaves, length and dry mass of root and shoots and percentage of death by necrosis. To the plantlets under different temperatures, length and dry mass of shoot and root, number of shoots, number, length and width of leaves and survival and rooting percentages were measured. The plantlets that were maintained under different luminosities were evaluated for length of shoot and root, dry mass of leaf and root. To evaluate the growth under the LEDs, the length and dry mass of shoot and root, number of shoots and roots, percentage of rooting and sprouting were assessed. The medium 1/4MS and the medium 1/2MS showed better responses for number and length of root, leaf width and shoot length. The temperature 25 provided the highest number of leaves, length of shoot and root, root dry mass and rooting percentage. The luminosity 73 μmol·m-2·s-1 provided higher values in length of root and dry mass of leaf and root. The red LED provided plantlets with greater growth. Thus, for the in vitro cultivation of Piper aduncum, 1/4MS, environment temperature of 25, light intensity of 73
Gas Exchange and Production of Photosynthetic Pigments of Piper aduncum L. Grown at Different Irradiances  [PDF]
Fernanda Ventorim Pacheco, Helbert Rezende de Oliveira Silveira, Amauri Alves Alvarenga, Ivan Caldeira Almeida Alvarenga, José Eduardo Brasil Pereira Pinto, Jean Marcel Sousa Lira
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.412A3014

The species Piper aduncum is a plant with great economic potential, because the essential oil has antimicrobial activity and insecticides. Thus, as the light directly affects photosynthesis process, the present study evaluated the gas exchanges variables and photosynthetic pigments production of P. aduncum grown under different irradiances. Treatments consisted in environments with 100%, 70% and 50% of irradiance and environments with colored nets (red and blue). After 150 days of culture, photosynthetic activity (A), stomatal conductance (gs) transpiration rate (E), internal CO2 concentration (Ci), deficit vapor pressure (DVP), leaf temperature, concentration of internal/external CO2 (Ci/Ca), carboxylation efficiency (A/Ci) and efficiency water use (A/E) were evaluated. Pigments chlorophyll a, b, carotenoids, total and chlorophyll a

época da indu??o e evoca??o floral em Citrus sinensis (L.) Osbeck cv. Pêra Rio
Pereira, Iara Alvarenga Mesquita;Pinto, José Eduardo Brasil Pereira;Davide, Lisete Chamma;
Ciência Rural , 2003, DOI: 10.1590/S0103-84782003000500010
Abstract: the research aims to determine the time in which the inflorescence induction and evocation occurs. the production of inflorescence stimulus (induction) and emission of first sepals in a bud determined as a flower bud (floral evocation) in citrus sinensis (l.) osbeck cv. pêra rio could be observed through the anatomic variations of the apical and lateral meristems. longitudinal sections, stained with pironina y-methylgreen were done in regular periods from may to august. the time of the year in which the vegetative buds are induced to blossom was determined through the bud shape changes, chiefly considering the diameter, which has increased with the induction. bud diameter variations were detected on the second half of july, the period of floral morphogenesis incipience. the measurement of the bud diameter is an efficient procedure to follow up the phenomena originated from the floral induction, evocation and morphogenesis. vegetative buds bring out an average diameter of 100μm with a three-layer tunic in a conic shape, recovering its body. the reproductive bud, in turn, is flatter, after the initiation of the first sepal primordium, showing an average diameter of 200μm. the pironina y-methylgreen stain provides the observation of the floral evocation phenomenon, through the detection of regions with a higher rna level, in the bud external areas. this biochemical alteration precedes the sepal emission.
Teor e composi o química do óleo essencial de Hyptis marrubioides Epl., Lamiaceae em fun o da sazonalidade = Content and chemical composition of Hyptis marrubioides essential oil in function of seasons
Priscila Pereira Botrel,José Eduardo Brasil Pereira Pinto,Vany Ferraz,Suzan Kelly Vilela Bertolucci
Acta Scientiarum : Agronomy , 2010,
Abstract: Os oleos essenciais sao principios odoriferos armazenados em celulas especiais da planta. O oleo essencial no genero Hyptis e usado como anestesico, antiespasmodico, antiinflamatorio e pode induzir aborto em doses elevadas. O objetivo deste trabalho foi avaliar a influencia da sazonalidade no teor e composicao do oleo essencial de Hyptis marrubioides. O delineamento experimental foi inteiramente casualizado, com quatro tratamentos (inverno, primavera, verao e outono) e seis repeticoes, totalizando 60 plantas. O oleo essencial foi extraido por hidrodestilacao, em aparelho de Clevenger. A analise qualitativa do oleo essencial foi por cromatografia em fase gasosa (CG/EM). Na estacao do verao, as folhas das plantas de H. marrubiodes apresentaram os maiores teores de oleo essencial. Nas demais estacoes, os teores de oleo essencial obtidos foram semelhantes entre si. Os componentes majoritarios no oleo essencial foram os monoterpenoides α-tujona e β-tujona. As concentracoes relativas dos picos correspondentes a β-tujona nao apresentaram diferencas consideraveis nas quatro estacoes. Os sesquiterpenoides oxigenados (cedrol e cariofilenol) e nao-oxigenados (α-copaeno, β-cariofileno, germacreno D e cadaleno) foram encontrados em menores quantidades no oleo essencial, entretanto observam-se diferencas quantitativas ao longo das estacoes. Essential oils are odorous principles stored in special plant cells. The essential oil in the Hyptis genus is traditionally used as an anesthetic, antispasmodic, antiinflammatory, and can induce abortion in high doses. The purpose of this work was to evaluate the influence of seasons in essential oil content and chemical composition of species H. marrubioides. A completely randomized complete design was used with four treatments (winter, spring, summer and autumn) and six replications, totaling 60 plants. The essential oil was obtained by hydrodistillation with a Clevenger apparatus. The qualitative analysis of the essential oil was performed by gas chromatography (GC/MS). In summer, plant leaves showed higher essential oil content. In the other seasons, essential oil content was similar. The majorcompounds in the essential oil were monoterpene α-thujone and β-thujone. The peak relative concentrations of β-thujone did not show considerable difference among the seasons. The oxygenate sesquiterpenoids (cedrol and caryophyllenol) and not oxygenate (α-copaene, α-caryophyllene, germacrene D and cadalene), were found in lower quantity in the essential oil; however, quantitative differences were observed over the seasons.
época da indu o e evoca o floral em Citrus sinensis (L.) Osbeck cv. Pêra Rio
Pereira Iara Alvarenga Mesquita,Pinto José Eduardo Brasil Pereira,Davide Lisete Chamma
Ciência Rural , 2003,
Abstract: A produ o do estímulo floral (indu o) e emiss o das primeiras sépalas, na gema floralmente determinada (evoca o floral) em Citrus sinensis (L.) Osbeck cv. Pêra Rio p de ser acompanhada pelas varia es anat micas nos meristemas apicais e axilares. Com objetivo de determinar a época na qual ocorre a indu o e evoca o floral, cortes longitudinais de gemas apicais e axilares, corados com pironina Y-methylgreen foram efetuados em períodos regulares de maio a agosto. Através das mudan as no formato da gema, principalmente diametro, que aumentou com a indu o, foi possível determinar a época do ano na qual gemas vegetativas s o induzidas a florescerem. Foram detectadas varia es nos diametros das gemas no período de início da morfogênese floral (meados até o final de julho). A medi o do diametro das gemas mostra-se um procedimento eficiente para acompanhar os fen menos decorrentes da indu o, evoca o e morfogênese florais. Gemas vegetativas apresentam diametro médio de 100μm, com túnica composta por três camadas, de formato c nico, recobrindo o corpo, ao passo que a reprodutiva é mais achatada, após a inicia o do primeiro primórdio de sépala, exibindo diametro médio de 200μm. Colora o com pironina Y-methylgreen proporciona a capta o do fen meno da evoca o floral, pela detec o de regi es mais concentradas em RNA, nas zonas periféricas das gemas; altera o bioquímica esta que precede a emiss o das sépalas.
Rela??o entre os estádios de desenvolvimento dos micrósporos e as características morfológicas do bot?o floral em piment?o (Capsicum annuum L.)
Luz, José Magno Queiroz;Silva, Renata;Davide, Lisete Chamma;Pinto, José Eduardo Brasil Pereira;
Ciência Rural , 1995, DOI: 10.1590/S0103-84781995000300009
Abstract: a number of factors influence the anther culture, among them, the developmental stage of microspores. the present study had the objective to verify the relationship among the developmental stage of microspores and a few morphological characteristics of the floral bud of the sweet pepper. floral buds of the genotypes: agron?mico 8, line 004 and the hybrid pix22c#31 x line 004, were divided into six classes of different sizes. length and diameter of the floral bud and the length of their anthers were determined, besides observing the presence of the anthocianm and relationship between the size of sepals and petals. the developmental stage of micropores was analysed through the coloration with acetic carmin. cytological analysis of each class showed that the non-vacuoled microspore, with central nucleus and diameter approximately 24,5 μm (suitable for use in anther culture), ocurred in every genotypes with petals of same size or a little bigger than the sepals and anthers with traces of anthocianin in their end.
Comprimento da estaca e tipo de substrato na propaga??o vegetativa de atroveran
Costa, Larissa Corrêa do Bomfim;Pinto, José Eduardo Brasil Pereira;Bertolucci, Suzan Kelly Vilela;
Ciência Rural , 2007, DOI: 10.1590/S0103-84782007000400040
Abstract: the vegetative propagation of medicinal species is in increasing agronomic interest because it is the starting point and a basic tool for any cultivation in commercial scale. the objective of this work was to determine the best shoot cutting length and the best substrate for vegetative propagation of ocimum selloi. cuttings were placed in greenhouse conditions under intermittent mist. two cutting sizes (10 and 20cm) and three substrate types (washed sand, carbonized rice hulls and commercial substrate plantmax?) were tested. the experiment was in blocks randomly distributed in four replications and five cuttings for parcel. after thirty five days the percentage of rooting, the length of the bigger root (cm) and the leaf and root dry weight (mg) were analysed. the results indicated that the vegetative propagation of ocimum selloi by cuttings is viable, once its mean rooting was over 70%. it was not observed significative interaction within the adopted treatments. the cuttings with 20cm length presented larger leaf and root dry weight but the percentage of rooting and the length of the bigger root was not affected by the cutting lenght. the substrate types did not present effect on the development of the cuttings. the production of ocimum selloi seedlings is recommended with 20cm length cuttings using any substrate tested.
Produ??o de mudas de hortel?-japonesa em fun??o da idade e de diferentes tipos de estaca
Chagas, Jorge Henrique;Pereira Pinto, José Eduardo Brasil;Bertolucci, Suzan Kelly Vilela;Nalon, Felipe Hébia;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000800011
Abstract: the objective of this research was to verify the influence of different age and types of cuttings in the production of scion of mentha arvensis l. four different types of cuttings were evaluated: apical and medium cuttings (from aerial part); tip and medium cuttings (from rhizome part). the different types of cuttings were evaluated at 25 and 40 days of age. the cuttings used had 4-5cm long and covered with plantmax? substrate to a depth of 2/3. two rooting periods (25 and 40 days) from the different types of cuttings were evaluated: height, shoots number, aerial and roots dry biomass and percentage of mortality. the cuttings after the rooting period were transplanted to bed and pots. the mortality and the aerial part, roots and rhizome dry biomass were determined 30 days after being cultivated. the results showed that apical cuttings from aerial part at 25 and 40 days after being planted in trays had higher rooting, aerial part development and low mortality. at 25 days the aerial part developed and the scion was ready to be transplanted to field. thirty days after transplanted apical cuttings from aerial part showed a higher growth, except for rhizome dry biomass.
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