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Search Results: 1 - 10 of 85513 matches for " José Augusto Nogueira-Machado "
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The Role of Oxidative Stress in the Aging Process
Barbara F. Oliveira,José Augusto Nogueira-Machado,Míriam M. Chaves
The Scientific World Journal , 2010, DOI: 10.1100/tsw.2010.94
Abstract:
Estresse oxidativo: revis?o da sinaliza??o metabólica no diabetes tipo 1
Reis, Janice Sepúlveda;Veloso, Clara Araújo;Mattos, Rafael Teixeira;Purish, Saulo;Nogueira-Machado, José Augusto;
Arquivos Brasileiros de Endocrinologia & Metabologia , 2008, DOI: 10.1590/S0004-27302008000700005
Abstract: diabetic complications appear to be multifactorial process. the biochemical and pathological mechanisms are associated with chronic hyperglycemia of diabetes and the increased oxidative stress which has been postulated to play a central role in these disorders. accumulating evidence suggests that oxidative cell injury caused by free radicals contributes to the development of type 1 diabetes (dm1) complications and decreased efficiency of antioxidant defenses (both enzymatic and nonenzymatic) seems to correlate with the severity of pathological tissue changes in dm1. in this review, we report as oxidative stress may exert deleterious effects in diabetes, as well as address current strategies in study to down-regulating vascular injury.
Effect of gamma radiation on the activity of hemocytes and on the course of Schistosoma mansoni infection in resistant Biomphalaria tenagophila snails
Bezerra Fernando Schemelzer de Moraes,Nogueira-Machado José Augusto,Martins-Souza Raquel Lopes,Chaves Miriam Martins
Memórias do Instituto Oswaldo Cruz , 2003,
Abstract: High doses of gamma radiation (10 Krad) in Biomphalaria tenagophila snails (Taim strain), which have been found to be resistant to Schistosoma mansoni, were not sufficient to impair their resistance to the parasite. The number of hemocytes, as well as their phagocytic activity, were not affected by irradiation, thus showing resemblance with mammal macrophages, which are resistant to gamma irradiation also.
Avalia??o da produ??o de espécies reativas de oxigênio por granulócitos de sangue periférico de pacientes com fibromialgia primária
Souza, Eduardo José do Rosário e;Nogueira-Machado, José Augusto;Silva, Francisco das Chagas Lima e;Chaves, Miriam Martins;Costa, Daniela Caldeira;
Revista Brasileira de Reumatologia , 2003, DOI: 10.1590/S0482-50042003000600003
Abstract: objective: our aim in this paper was to evaluate the biochemical abnormalities that may be correlated with fibromyalgia, such as the ros production by granulocytes from patients with fibromyalgia in comparison to cells from healthy subjects. methods: ten patients (9 women and 1 man, age ranging from 20 to 40 years old) with primary fibromyalgia, according to the american college of rheumatology criteria, were selected. none of them were using medications 2 weeks before starting the study. patients with rheumatic and non-rheumatic associated disease were excluded. sex and age-matched healthy controls (n = 19) were simultaneously selected. the granulocytes from patients and controls were collected from peripheral blood. the quantification of reactive oxygen species was performed in a luminol-dependent chemiluminescence assay. results: we have demonstrated, for the first time, the increase of ros production (3.2 times) in peripheral blood granulocytes from patients with fibromyalgia in comparison to the same cells from healthy subjects. conclusions: the present result may indicate a possible role of free radicals in fibromyalgia subjects, once they have already been shown in other human diseases, including painful states. we suggest that further studies are needed to understand the importance of these findings, delineating a new perspective in understanding and approach of fibromyalgia patients.
cAMP Activates The generation of Reactive Oxygen Species and Inhibits The Secretion of IL-6 in Peripheral Blood Mononuclear Cells from Type 2 Diabetic Patients
Camila Armond Isoni,érica Abreu Borges,Clara Araújo Veloso,Rafael Teixeira Mattos,Miriam Martins Chaves,José Augusto Nogueira-Machado
Oxidative Medicine and Cellular Longevity , 2009, DOI: 10.4161/oxim.2.5.9657
Abstract: Peripheral blood mononuclear cells (PBMNC) from patients with type 2 diabetes (DM2) have generated higher levels of reactive oxygen species (ROS) that were higher than those in cells from healthy individuals. In the presence of a cAMP-elevating agent, ROS production was significantly activated in PBMNC from DM2 patients but it was inhibited in cells from healthy subjects. Higher levels of IL-6 has been detected in the supernatant of PBMNC cultures from DM2 patients in comparison with healthy controls. When cells were cultured in the presence of a cAMP-elevating agent, the level of IL-6 decreased has by 46% in the supernatant of PBMNC from DM2 patients but it remained unaltered in controls. No correlations between ROS and IL-6 levels in PBMNC from DM2 patients or controls have been observed. Secretions of IL-4 or IFN by PBMNC from patients or controls have not been affected by the elevation of cAMP. cAMP elevating agents have activated the production of harmful reactive oxidant down modulated IL-6 secretion by these cells from DM2 patients, suggesting an alteration in the metabolic response possibly due to hyperglicemia. The results suggest that cAMP may play an important role in the pathogenesis of diabetes.
Effect of gamma radiation on the activity of hemocytes and on the course of Schistosoma mansoni infection in resistant Biomphalaria tenagophila snails
Bezerra, Fernando Schemelzer de Moraes;Nogueira-Machado, José Augusto;Martins-Souza, Raquel Lopes;Chaves, Miriam Martins;Correa, Ricardo Ferracini;Coelho, Paulo Marcos Zech;
Memórias do Instituto Oswaldo Cruz , 2003, DOI: 10.1590/S0074-02762003000100010
Abstract: high doses of gamma radiation (10 krad) in biomphalaria tenagophila snails (taim strain), which have been found to be resistant to schistosoma mansoni, were not sufficient to impair their resistance to the parasite. the number of hemocytes, as well as their phagocytic activity, were not affected by irradiation, thus showing resemblance with mammal macrophages, which are resistant to gamma irradiation also.
QUANTIFICATION OF THE POPULATION AND PHAGOCYTARY ACTIVITY OF HEMOCYTES OF RESISTANT AND SUSCEPTIBLE STRAINS OF Biomphalaria glabrata AND Biomphalaria tenagophila INFECTED WITH Schistosoma mansoni
BEZERRA F. S. de M.,NOGUEIRA-MACHADO J. A.,CHAVES M. M.,MARTINS R. L.
Revista do Instituto de Medicina Tropical de S?o Paulo , 1997,
Abstract: Among the determinant factors in the resistance and susceptibility of Biomphalaria to Schistosoma mansoni, hemocytes play an important role. Aiming at studying S. mansoni/Biomphalaria interactions related to hemocytes, the first step is certainly connected with the standardization of this cell population in uninfected Biomphalaria. In this way, quantification of this cell population in hemolymph, as well as its phagocitary capacity, have been determined for the first time. Furthermore, using susceptible and resistant strains of B. glabrata and B. tenagophila, the hemocytegram and phagocytary capacity of hemocytes after infection with S. mansoni were determined too. Resistant and susceptible strains of B.glabrata (BA and BH, respectively), as well as resistant and susceptible strains of B. tenagophila (Taim and CF, respectively) were infected with 10 miracidia of the LE and SJ strains of S. mansoni, respectively. These infected snails and respective uninfected controls were assessed in relation to the number of circulating hemocytes and alteration in the phagocytary capacity, by using Zymozan and MTT. Reading was taken by means of a spectrophotometer at 5 hours and 1,2,5,10,20 and 30 days after infection. The results showed a decrease in population of the circulating phagocytary cells, 5 hours after infection. One day post-infection, the circulating cells of the susceptible snails showed an increased metabolic activity, but the same event could not be observed in the resistant strains. In the subsequent observation periods, significant differences among the strains studied could not be observed until the end of the experiment
RESISTANCE TO OXAMNIQUINE OF A Schistosoma mansoni STRAIN ISOLATED FROM PATIENT SUBMITTED TO REPEATED TREATMENTS
COELHO M.Z.,LIMA E SILVA F.C.,NOGUEIRA-MACHADO J.A.
Revista do Instituto de Medicina Tropical de S?o Paulo , 1997,
Abstract: A strain of Schistosoma mansoni (R1) was isolated from patient previously submitted to four treatments with oxamniquine, and to another one with praziquantel. The results obtained with chemotherapeutic test, by using oxamniquine in mice infected with the strains R1 and LE (standard), showed an evident resistance to the drug in worms of the strain R1. Thus, at the dose of 250 mg/kg oxamniquine, all mice (17) infected with the LE strain did not show surviving worms, whereas 12 out of 17 mice infected with the R1 strain presented surviving worms. At the dose of 200 mg/kg, the LE strain showed recovery rates of 1.06% and 20.58%, whereas the R1 strain presented 18.57% and 61.14%, for male and female worms, respectively. At the dose of 100 mg/kg, the recovery of male worms was 2.6% for the LE strain, and 29.9% for the R1 strain. At the same dose, the recovery of females did not show statistically significant differences between the two strains (LE = 76.38%, R1 = 79.12%). Praziquantel showed similar antischistosomal activity against both studied strains, when administered at the dose of 500 mg/kg
QUANTIFICATION OF THE POPULATION AND PHAGOCYTARY ACTIVITY OF HEMOCYTES OF RESISTANT AND SUSCEPTIBLE STRAINS OF Biomphalaria glabrata AND Biomphalaria tenagophila INFECTED WITH Schistosoma mansoni
BEZERRA, F. S. de M.;NOGUEIRA-MACHADO, J. A.;CHAVES, M. M.;MARTINS, R. L.;COELHO, P. M. Z.;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1997, DOI: 10.1590/S0036-46651997000400003
Abstract: among the determinant factors in the resistance and susceptibility of biomphalaria to schistosoma mansoni, hemocytes play an important role. aiming at studying s. mansoni/biomphalaria interactions related to hemocytes, the first step is certainly connected with the standardization of this cell population in uninfected biomphalaria. in this way, quantification of this cell population in hemolymph, as well as its phagocitary capacity, have been determined for the first time. furthermore, using susceptible and resistant strains of b. glabrata and b. tenagophila, the hemocytegram and phagocytary capacity of hemocytes after infection with s. mansoni were determined too. resistant and susceptible strains of b.glabrata (ba and bh, respectively), as well as resistant and susceptible strains of b. tenagophila (taim and cf, respectively) were infected with 10 miracidia of the le and sj strains of s. mansoni, respectively. these infected snails and respective uninfected controls were assessed in relation to the number of circulating hemocytes and alteration in the phagocytary capacity, by using zymozan and mtt. reading was taken by means of a spectrophotometer at 5 hours and 1,2,5,10,20 and 30 days after infection. the results showed a decrease in population of the circulating phagocytary cells, 5 hours after infection. one day post-infection, the circulating cells of the susceptible snails showed an increased metabolic activity, but the same event could not be observed in the resistant strains. in the subsequent observation periods, significant differences among the strains studied could not be observed until the end of the experiment
Is CK-MB isoenzyme useful for diagnosis of cardiac involvement in icteric leptospirosis?
Machado, Elizabeth Stankiewicz;Feres, José Guilherme de Faria;Feijó, Luis Augusto;Andrade, Jarbas;Nogueira, Susie Andries;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1995, DOI: 10.1590/S0036-46651995000500013
Abstract: in the absence of heart failure or cardiogenic shock, cardiac involvement diagnosis in icteric leptospirosis is possible on the basis of abnormal electrocardiograms. as metabolic and electrolytic disorders are frequently seen during acute leptospirosis infection, they may be responsible for some electrocardiograms changes. we conducted a study to assess if creatine phosphokinase isoenzyme determinations are useful in selecting patients with a high cardiac involvement suspicion. sixty-nine patients were studied prospectively. ten patients out of 16 with cardiac involvement and 25 without had high ck-mb levels (p>0.05), although mean values of abnormal ck-mb levels were higher in the group with cardiac involvement (p<0.05). our analysis indicates that serum ck-mb determination does not provide a specific indicator of myocardial involvement in the course of icteric leptospirosis.
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