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Search Results: 1 - 10 of 21064 matches for " Jonas Moraes-Filho "
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Serosurvey of Rickettsia spp. in dogs and humans from an endemic area for Brazilian spotted fever in the State of S?o Paulo, Brazil
Pinter, Adriano;Horta, Maurício C.;Pacheco, Richard C.;Moraes-Filho, Jonas;Labruna, Marcelo B.;
Cadernos de Saúde Pública , 2008, DOI: 10.1590/S0102-311X2008000200003
Abstract: the present study provides a rickettsial serosurvey in 25 dogs and 35 humans in an endemic area for brazilian spotted fever in the state of s?o paulo, where the tick amblyomma aureolatum is the main vector. testing canine and human sera by indirect immunofluorescence against four rickettsia antigens (r. rickettsii, r. parkeri, r. felis and r. bellii) showed that 16 (64%) of canine sera and 1 (2.8%) of human sera reacted to at least one of these rickettsial antigens with titers 3 64. seven canine sera and the single reactive human serum showed titers to r. rickettsii at least four times those of any of the other three antigens. the antibody titers in these 7 animals and 1 human were attributed to stimulation by r. rickettsii infection. no positive canine or human serum was attributed to stimulation by r. parkeri, r. felis, or r. bellii. our serological results showed that dogs are important sentinels for the presence of r. rickettsii in areas where the tick a. aureolatum is the main vector of brazilian spotted fever.
Serological evidence of Rickettsia parkeri as the etiological agent of rickettsiosis in Uruguay
Conti-Díaz, Ismael A.;Moraes-Filho, Jonas;Pacheco, Richard C.;Labruna, Marcelo B.;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2009, DOI: 10.1590/S0036-46652009000600005
Abstract: we report three new rickettsiosis human cases in uruguay. the three clinical cases presented clinical manifestations similar to previous reported cases of rickettsia parkeri in the united states; that is mild fever (< 40 oc), malaise, headache, rash, inoculation eschar at the tick bite site, regional lymphadenopathy, and no lethality. serological antibody-absorption tests with purified antigens of r. parkeri and rickettsia rickettsii, associated with immunofluorescence assay indicated that the patients in two cases were infected by r. parkeri. epidemiological and clinical evidences, coupled with our serological analysis, suggest that r. parkeri is the etiological agent of human cases of spotted fever in uruguay, a disease that has been recognized in that country as cutaneous-ganglionar rickettsiosis.
Molecular detection of Ehrlichia canis in dogs from the Pantanal of Mato Grosso State, Brazil Detec o molecular de Ehrlichia canis em c es do Pantanal do Mato Grosso, Brasil
Luana Gabriela Ferreira dos Santos,Andréia Lima Tomé Melo,Jonas Moraes-Filho,Rute Witter
Revista Brasileira de Parasitologia Veterinária , 2013,
Abstract: The present study evaluated the presence of Ehrlichia DNA in the blood samples of 320 dogs from the urban and rural areas of the municipality of Poconé, Pantanal region, Mato Grosso state, by Polymerase Chain Reaction (PCR), targeting the ehrlichial dsb gene. Risk factors for infection in dogs were also evaluated. Forty-eight (15%, 95% CI: 11.4-19.5%) dogs were positive: 25 (15.6%, 95% CI: 10.4-22.2%) from the urban area and 23 (14.4%, 95% CI: 9.3-20.8%) from the rural area (P > 0.05). Partial DNA sequence obtained from PCR products of 18 samples from the urban area and 16 samples from the rural area were 100% identical to E. canis from Brazil and the USA. This study reports the first E. canis molecular detection in dogs from the northern Pantanal region. O presente estudo avaliou a presen a de DNA de Ehrlichia spp. em 320 c es das áreas urbana e rural do município de Poconé, regi o do Pantanal de Mato Grosso, pela PCR visando o gene dsb. Os fatores de risco para a infec o em c es também foram avaliados. Quarenta e oito (15%, IC 95%: 11,4-19,5%) c es foram positivos, 25 (15,6%, IC 95%: 10,4-22,2%) da área urbana e 23 (14,37%, 95% CI: 9,3-20,8%) da área rural (P > 0,05). Sequências parciais de DNAs obtidos a partir de produtos da PCR de 18 amostras da área urbana e 16 da área rural foram 100% idênticas a E. canis do Brasil e EUA. Este estudo relata a primeira detec o molecular de E. canis em c es da regi o norte do Pantanal.
Infección por rickettsia en capibaras (Hydrochoerus hydrochaeris) de S o Paulo, Brasil: evidencia serológica de infección por Rickettsia bellii y Rickettsia parkeri Rickettsial infection in capybaras (Hydrochoerus hydrochaeris from S o Paulo, Brazil: serological evidence for infection by Rickettsia bellii and Rickettsia parkeri
Richard C. Pacheco,Mauricio C. Horta,Jonas Moraes-Filho,Alexandre C. Ataliba
Biomédica , 2007,
Abstract: Introducción. En Brasil, los capibaras (Hydrochoerus hydrochaeris) son importantes huéspedes para garrapatas del género Amblyomma, las cuales transmiten rickettsiosis a humanos y animales. Por lo tanto, estos roedores pueden ser potenciales centinelas para detectar infección por rickettsia. Objetivos. Este trabajo evaluó la infección por rickettsia en capibaras de diferentes regiones del estado de S o Paulo, donde las rickettsiosis nunca han sido reportadas. Materiales y métodos. Se examinarion los sueros de 73 capibaras de seis localidades en S o Paulo con la prueba de immunofluorescencia indirecta con antígenos de Rickettsia rickettsii, Rickettsia parkeri y Rickettsia bellii. Los bazos de los capibaras se extrajeron y se analizaron por reacción en cadena de la polimerasa para un fragmento del gene gltA de rickettsia. Las garrapatas se recolectaron de los capibaras y se identificaron hasta especie. Resultados. Diecinueve (26,0%), 25 (34,2%) y 50 (68,5%) sueros de los capibaras reaccionaron con R. rickettsii, R. parkeri y R. bellii, respectivamente. De los 50 sueros que reaccionaron con antígenos de R. bellii, 25 presentaron títulos, por lo menos, cuatro veces mayores que los otros dos antígenos. Estos sueros fueron considerados homólogos de R. bellii. Usando el mismo criterio, tres sueros de los capibaras se consideraron homólogos de R. parkeri. Ningún suero se consideró homólogo de R. rickettsii. No se detectó ADN de rickettsia en bazo. Las garrapatas recolectadas de los capibaras fueron identificadas como Amblyomma dubitatum y Amblyomma cajennense. Conclusiones. Este trabajo reporta la primera evidencia de infección natural por R. bellii en vertebrados y, también, la primera evidencia de infección por R. parkeri en capibaras. Se sabe que R. parkeri infecta y produce enfermedad en humanos; sin embargo, no hay evidencia de infección humana por R. bellii. Introduction. In Brazil, capybaras (Hydrochoerus hydrochaeris) are important hosts for Amblyomma ticks, which in turn can transmit rickettsiae to humans and animals. Therefore, capybaras are potential sentinels for rickettsial infection. Objective. The present study evaluated rickettsial infection in capybaras in different areas of the state of S o Paulo, where rickettsiosis has never been reported. Materials and methods. Blood sera from 73 capybaras from six localities in S o Paulo were tested by indirect immunofluorescence assay using Rickettsia rickettsii, Rickettsia parkeri, and Rickettsia bellii antigens. Capybara spleens were tested by PCR, targeting a fragment of the rickettsial gltA gene. Ticks
Rickettsial infection in capybaras (Hydrochoerus hydrochaeris) from S?o Paulo, Brazil: serological evidence for infection by Rickettsia bellii and Rickettsia parkeri
Pacheco,Richard C; Horta,Mauricio C; Moraes-Filho,Jonas; Ataliba,Alexandre C; Pinter,Adriano; Labruna,Marcelo B;
Biomédica , 2007,
Abstract: introduction. in brazil, capybaras (hydrochoerus hydrochaeris) are important hosts for amblyomma ticks, which in turn can transmit rickettsiae to humans and animals. therefore, capybaras are potential sentinels for rickettsial infection. objective. the present study evaluated rickettsial infection in capybaras in different areas of the state of s?o paulo, where rickettsiosis has never been reported. materials and methods. blood sera from 73 capybaras from six localities in s?o paulo were tested by indirect immunofluorescence assay using rickettsia rickettsii, rickettsia parkeri, and rickettsia bellii antigens. capybara spleens were tested by pcr, targeting a fragment of the rickettsial glta gene. ticks were collected from each capybara sample and taxonomically identified to species. results. a total of 94 positively reacting capybara samples, 19 (26.0%), 25 (34.2%), and 50 (68.5%) capybara sera reacted to r. rickettsii, r. parkeri, and r. bellii, respectively. twenty-five capybara sera showed titers to r. bellii at least four-fold higher than to any of the other two antigens. these sera were considered homologous to r. bellii. using the same criteria, 3 capybara sera were considered homologous to r. parkeri. no sera were be considered homologous to r. rickettsii. no rickettsial dna was detected in capybara spleen samples. ticks collected on capybaras were amblyomma dubitatum and amblyomma cajennense. conclusions. the first evidence is reported of r. bellii natural infection in vertebrate hosts, and the first evidence of r. parkeri infection in capybaras. while r. parkeri is known to infect and cause disease in humans, no similar evidence for human infection has been indicated by r. bellii.
Seroprevalence of Rickettsia bellii and Rickettsia felis in dogs, S?o José dos Pinhais, State of Paraná, Brazil
Fortes, Fernanda Silva;Silveira, Iara;Moraes-Filho, Jonas;Leite, Ronaldo Viana;Bonacim, José Edivaldo;Biondo, Alexander Welker;Labruna, Marcelo Bahia;Molento, Marcelo Beltr?o;
Revista Brasileira de Parasitologia Veterinária , 2010, DOI: 10.1590/S1984-29612010000400006
Abstract: brazilian spotted fever (bsf) is a vector-borne zoonosis caused by rickettsia rickettsii bacteria. dogs can be host sentinels for this bacterium. the aim of the study was to determine the presence of antibodies against rickettsia spp. in dogs from the city of s?o josé dos pinhais, state of paraná, southern brazil, where a human case of bsf was first reported in the state. between february 2006 and july 2007, serum samples from 364 dogs were collected and tested at 1:64 dilutions by indirect immunofluorescence assay (ifa) against r. rickettsii and r. parkeri. all sera that reacted at least to one of rickettsia species were tested against the six main rickettsia species identified in brazil: r. rickettsii, r. parkeri, r. bellii, r. rhipicephali, r. amblyommii and r. felis. sixteen samples (4.4%) reacted to at least one rickettsia species. among positive animals, two dogs (15.5%) showed suggestive titers for r. bellii exposure. one sample had a homologous reaction to r. felis, a confirmed human pathogen. although rickettsia spp. circulation in dogs in the area studied may be considered at low prevalence, suggesting low risk of human infection, the present data demonstrate for the first time the exposure of dogs to r. bellii and r. felis in southern brazil.
Brazilian spotted fever in cart horses in a non-endemic area in Southern Brazil
Freitas, Marta Cristina Diniz de Oliveira;Grycajuk, Marcelly;Molento, Marcelo Beltr?o;Bonacin, José;Labruna, Marcelo Bahia;Pacheco, Richard de Campos;Moraes-Filho, Jonas;Deconto, Ivan;Biondo, Alexander Welker;
Revista Brasileira de Parasitologia Veterinária , 2010, DOI: 10.4322/rbpv.01902013
Abstract: brazilian spotted fever (bsf) is an often fatal zoonosis caused by the obligate intracellular bacterium rickettsia rickettsii. the disease is generally transmitted to humans by amblyomma spp. ticks. serological evidence of past infection by r. rickettsii has been reported in horses, but the pathogenicity of r. rickettsii in horses remains unknown. cart horses are still widely used in urban and urban fringe areas in brazil, and these animals may constitute suitable sentinels for bsf human in these areas, for example, in sao jose dos pinhais, where the first bsf human case in the state of parana was diagnosed. serum samples were randomly obtained from 75 cart horses between april 2005 and june 2006 and were tested by means of the indirect immunofluorescence assay (ifa) for antibodies against rickettsia of the spotted fever group. a total of 9.33% of the animals were considered positive, with titers ranging from 64 to 1,024. these results indicate the presence of the agent in such areas, although at low rates.
Pesquisa de Rickettsia spp em carrapatos Amblyomma cajennense e Amblyomma dubitatum no Estado de S?o Paulo
Pacheco, Richard Campos;Horta, Maurício Cláudio;Pinter, Adriano;Moraes-Filho, Jonas;Martins, Thiago Fernandes;Nardi, Marcello Schiavo;Souza, Savina Silvana Aparecida Lacerra de;Souza, Celso Eduardo de;Szabó, Matias Pablo Juan;Richtzenhain, Leonardo José;Labruna, Marcelo Bahia;
Revista da Sociedade Brasileira de Medicina Tropical , 2009, DOI: 10.1590/S0037-86822009000300023
Abstract: the presence of rickettsial infection was surveyed in 3,545 amblyomma cajennense ticks and 2,666 amblyomma dubitatum ticks. using the hemolymph test, polymerase chain reaction and isolation of rickettsia in cell cultures, all of the amblyomma cajennense were negative, whereas 634 (23.8%) of the amblyomma dubitatum ticks were shown to be infected with rickettsia bellii.
Serological survey of Rickettsia sp. in horses and dogs in an non-endemic area in Brazil
Batista, Fernanda Gon?alves;Silva, Daniella Matos da;Green, Kerriel Thandile;Tezza, Louise Boulsfield de Lorenzi;Vasconcelos, Samara Pereira de;Carvalho, Suelen Graziele Soares de;Silveira, Iara;Moraes-Filho, Jonas;Labruna, Marcelo Bahia;Fortes, Fernanda Silva;Molento, Marcelo Beltr?o;
Revista Brasileira de Parasitologia Veterinária , 2010, DOI: 10.1590/S1984-29612010000400003
Abstract: brazilian spotted fever (bsf) is a lethal rickettsiosis in humans caused by the bacteria rickettsia rickettsii, and is endemic in some areas of brazil. horses and dogs are part of the disease's life cycle and they may also serve as sentinel animals in epidemiological studies. the first human bsf case in the state of paraná was reported in 2005. the present study was conducted in the municipality of almirante tamandaré, where no previous case of bsf was reported. serum samples were collected from 71 horses and 20 dogs from nine properties in the area. ticks were also collected from these animals. all farmers completed a questionnaire about their knowledge of bsf and animal health management. serum samples were analyzed by indirect immunofluorescent-antibody assay (ifa) using r. rickettsii and r. parkeri as antigens. ticks were analyzed by pcr for rickettsia sp., and all of them were pcr-negative. six horses (8.45%) and 4 dogs (20%) were identified as seropositive. farmers were not aware of the correlation between the presence of ticks and risk of bsf. although a non-endemic area, almirante tamandaré is a vulnerable environment for bsf and effective tick control measures are required.
Dor torácica n?o-cardiogênica
Domingues, Gerson Ricardo de Souza;Moraes-Filho, Joaquim Prado P.;
Arquivos de Gastroenterologia , 2009, DOI: 10.1590/S0004-28032009000300018
Abstract: context: non-cardiac chest pain or functional chest pain is a syndrome with high prevalence in ocidental world. findings on 15%-30% of coronary angiograms performed in patients with chest pain are normal. causes significant impact in quality of life of patients and is associated with increased use of the health care facilities. data sources: to this review the following data base were accessed: medline, the cochrane library, lilacs. the limit was the last 5 years publications and were selected relevant original articles, reviews, consensus, guidelines and meta-analysis. results: forty-four papers were selected, 28 original articles, 12 reviews, 2 guidelines, 1 consensus and 1 meta-analysis. conclusions: exclusion of cardiac disease is of crucial importance. on the other hand non-cardiac chest pain could be related to gastrointestinal, muscular and respiratory causes and/or psychological disturbances. treatment aims to attack mechanism generator in order to relieve or to eliminate symptoms. drugs are the cornerstone of treatment, exception to achalasia patients because those have better response to dilation of the esophagus or surgery, and to those who need intensive pyschological therapy. the most important drugs used are proton pump inhibitors and triciclic antidepressants, the latter, to modulate central signal process (visceral hypersensitivity) and autonomic response. recently, new diagnostic facilities, and also therapeutic modalities, such as esophageal botulin toxin injection and hypnosis are under investigations. in the near future, maybe some of them would take a place in the therapeutic scenario of these patients.
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