oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

4 ( 1 )

2020 ( 4 )

2019 ( 276 )

2018 ( 301 )

Custom range...

Search Results: 1 - 10 of 135367 matches for " Jo?o Ramalho-Carvalho "
All listed articles are free for downloading (OA Articles)
Page 1 /135367
Display every page Item
Cysteine-Rich Secretory Protein-3 (CRISP3) Is Strongly Up-Regulated in Prostate Carcinomas with the TMPRSS2-ERG Fusion Gene
Franclim R. Ribeiro,Paula Paulo,Vera L. Costa,Joo D. Barros-Silva,Joo Ramalho-Carvalho,Carmen Jerónimo,Rui Henrique,Guro E. Lind,Rolf I. Skotheim,Ragnhild A. Lothe,Manuel R. Teixeira
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0022317
Abstract: A large percentage of prostate cancers harbor TMPRSS2-ERG gene fusions, leading to aberrant overexpression of the transcription factor ERG. The target genes deregulated by this rearrangement, however, remain mostly unknown. To address this subject we performed genome-wide mRNA expression analysis on 6 non-malignant prostate samples and 24 prostate carcinomas with (n = 16) and without (n = 8) TMPRSS2-ERG fusion as determined by FISH. The top-most differentially expressed genes and their associations with ERG over-expression were technically validated by quantitative real-time PCR and biologically validated in an independent series of 200 prostate carcinomas. Several genes encoding metabolic enzymes or extracellular/transmembrane proteins involved in cell adhesion, matrix remodeling and signal transduction pathways were found to be co-expressed with ERG. Within those significantly over-expressed in fusion-positive carcinomas, CRISP3 showed more than a 50-fold increase when compared to fusion-negative carcinomas, whose expression levels were in turn similar to that of non-malignant samples. In the independent validation series, ERG and CRISP3 mRNA levels were strongly correlated (rs = 0.65, p<0.001) and both were associated with pT3 disease staging. Furthermore, immunohistochemistry results showed CRISP3 protein overexpression in 63% of the carcinomas and chromatin immunoprecipitation with an anti-ERG antibody showed that CRISP3 is a direct target of the transcription factor ERG. We conclude that ERG rearrangement is associated with significant expression alterations in genes involved in critical cellular pathways that define a subset of locally advanced PCa. In particular, we show that CRISP3 is a direct target of ERG that is strongly overexpressed in PCa with the TMPRSS2-ERG fusion gene.
Promoter methylation and large intragenic rearrangements of DPYD are not implicated in severe toxicity to 5-fluorouracil-based chemotherapy in gastrointestinal cancer patients
Joana Savva-Bordalo, Joo Ramalho-Carvalho, Manuela Pinheiro, Vera L Costa, ?ngelo Rodrigues, Paula C Dias, Isabel Veiga, Manuela Machado, Manuel R Teixeira, Rui Henrique, Carmen Jerónimo
BMC Cancer , 2010, DOI: 10.1186/1471-2407-10-470
Abstract: In this study, we evaluated DPYD promoter methylation through quantitative methylation-specific PCR and screened DPYD for large intragenic rearrangements in peripheral blood from 45 patients with gastrointestinal cancers who developed severe 5-FU toxicity. DPYD promoter methylation was also assessed in tumor tissue from 29 patientsTwo cases with the IVS14+1G > A exon 14 skipping mutation (c.1905+1G > A), and one case carrying the 1845 G > T missense mutation (c.1845G > T) in the DPYD gene were identified. However, DPYD promoter methylation and large DPYD intragenic rearrangements were absent in all cases analyzed.Our results indicate that DPYD promoter methylation and large intragenic rearrangements do not contribute significantly to the development of 5-FU severe toxicity in gastrointestinal cancer patients, supporting the need for additional studies on the mechanisms underlying genetic susceptibility to severe 5-FU toxicity.The fluoropyrimidine 5-fluorouracil (5-FU) is broadly used in the treatment of a wide range of gastrointestinal cancers[1]. 5-FU is an analogue of uracil with a fluorine atom at the C-5 position replacing hydrogen, which enters the cell using the same transport machinery as the uracil nucleotide[2]. The mechanism of 5-FU cytotoxicity has been ascribed to the inhibition of thymidylate synthase (TYMS) and misincorporation of fluoronucleotides into RNA and DNA [1,3]. The rate-limiting enzyme in 5-FU catabolism is dihydropyrimidine dehydrogenase (DPD), which converts 5-FU to dihydrofluorouracil. More than 80% of administered 5-FU is normally catabolized primarily in the liver, where DPD is abundantly expressed[1].The types of toxicity associated with 5-FU are predominantly myelosuppression, diarrhea, mucositis, and hand-foot syndrome[4,5]. The mechanism of 5-FU cytotoxicity may depend on genetic and clinical factors. Female gender and mode of administration in bolus are linked to increased toxicity[6]. In 5-FU bolus schedules, the incorporation of
Altered Expression of MGMT in High-Grade Gliomas Results from the Combined Effect of Epigenetic and Genetic Aberrations
Joo Ramalho-Carvalho, Malini Pires, Susana Lisboa, Inês Gra?a, Patrícia Rocha, Joo Diogo Barros-Silva, Joana Savva-Bordalo, Joaquina Maurício, Mário Resende, Manuel R. Teixeira, Mrinalini Honavar, Rui Henrique, Carmen Jerónimo
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0058206
Abstract: MGMT downregulation in high-grade gliomas (HGG) has been mostly attributed to aberrant promoter methylation and is associated with increased sensitivity to alkylating agent-based chemotherapy. However, HGG harboring 10q deletions also benefit from treatment with alkylating agents. Because the MGMT gene is mapped at 10q26, we hypothesized that both epigenetic and genetic alterations might affect its expression and predict response to chemotherapy. To test this hypothesis, promoter methylation and mRNA levels of MGMT were determined by quantitative methylation-specific PCR (qMSP) or methylation-specific multiplex ligation dependent probe amplification (MS-MLPA) and quantitative RT-PCR, respectively, in a retrospective series of 61 HGG. MGMT/chromosome 10 copy number variations were determined by FISH or MS-MLPA analysis. Molecular findings were correlated with clinical parameters to assess their predictive value. Overall, MGMT methylation ratios assessed by qMSP and MS-MLPA were inversely correlated with mRNA expression levels (best coefficient value obtained with MS-MLPA). By FISH analysis in 68.3% of the cases there was loss of 10q26.1 and in 15% of the cases polysomy was demonstrated; the latter displayed the highest levels of transcript. When genetic and epigenetic data were combined, cases with MGMT promoter methylation and MGMT loss depicted the lowest transcript levels, although an impact in response to alkylating agent chemotherapy was not apparent. Cooperation between epigenetic (promoter methylation) and genetic (monosomy, locus deletion) changes affecting MGMT in HGG is required for effective MGMT silencing. Hence, evaluation of copy number alterations might add relevant prognostic and predictive information concerning response to alkylating agent-based chemotherapy.
Clinical and radiographic evaluation of a polyester prosthesis in dogs with cranial cruciate ligament rupture
Selmi André Luis,Padilha Filho Joo Guilherme,Barbudo Glenda Ramalho,Buquera Luiz Eduardo Carvalho
Ciência Rural , 2002,
Abstract: Clinical and radiographic findings after intra-articular replacement of cranial cruciate ligament with a polyester prosthesis using a modified over-the-top technique were evaluated in six dogs. Seven surgeries were done due to bilateral involvement of the cranial cruciate ligament in one dog. Clinical evaluation was performed on postoperative days 3, 10 and 40, and radiographic evaluation was done at 5 and 24 months after surgery in five dogs, where signs of progressive degenerative joint disease were confirmed. Resolution of clinical signs was observed from 25 to 68 days after surgery as evaluated by dog owners. Overall function of joint movement after surgery was classified as good. Two dogs presented fraying of the implant after surgery. It was concluded that the polyester prosthesis, as implanted in these dogs, was not a satisfactory replacement for the injured ligament, as better results may be obtained with less invasive and simpler techniques.
Clinical and radiographic evaluation of a polyester prosthesis in dogs with cranial cruciate ligament rupture
Selmi, André Luis;Padilha Filho, Joo Guilherme;Barbudo, Glenda Ramalho;Buquera, Luiz Eduardo Carvalho;Canola, Júlio Carlos;
Ciência Rural , 2002, DOI: 10.1590/S0103-84782002000500009
Abstract: clinical and radiographic findings after intra-articular replacement of cranial cruciate ligament with a polyester prosthesis using a modified over-the-top technique were evaluated in six dogs. seven surgeries were done due to bilateral involvement of the cranial cruciate ligament in one dog. clinical evaluation was performed on postoperative days 3, 10 and 40, and radiographic evaluation was done at 5 and 24 months after surgery in five dogs, where signs of progressive degenerative joint disease were confirmed. resolution of clinical signs was observed from 25 to 68 days after surgery as evaluated by dog owners. overall function of joint movement after surgery was classified as good. two dogs presented fraying of the implant after surgery. it was concluded that the polyester prosthesis, as implanted in these dogs, was not a satisfactory replacement for the injured ligament, as better results may be obtained with less invasive and simpler techniques.
New Brazilian rules for herbal medicines
Ana Cecília BEZERRA CARVALHO,Joo Paulo SILVéRIO PERFEITO,Leandro Viana COSTA E SILVA,Livia SANTOS RAMALHO
Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas , 2010,
Abstract: Brasil acaba de publicar varias normas relacionadas con las plantas medicinales y fitoterápicos con el fin de satisfacer las demandas de las políticas públicas vigentes en el país y fomentar la entrada de medicamentos a base de hierbas con la seguridad, eficacia y calidad para la población. Este artículo presenta las normas recientemente publicadas y presenta los cambios más importantes.
Disposi??o de adolescentes para a prática de esportes: um estudo orientado pela teoria bioecológica de Bronfenbrenner
Krebs, Ruy Jornada;Ramalho, Maria Helena da Silva;Santos, Joo Otacilio Libardoni dos;Nazario, Patrik Felipe;Nobre, Glauber Carvalho;Almeida, Raquel Teixeira;
Motriz: Revista de Educa??o Física , 2011, DOI: 10.5016/1980-6574.2011v17n1p195
Abstract: the present study aimed to analyze the adolescent's disposition for sports based in the bioecological paradigm of bronfenbrenner. the design of this study include two contexts (microsystems) with different environmental characteristics: (a) public school and (b) private school, both located on downtown of florianopolis. the results were organized according to two variables of the study: personal attributes (male or female) and context (public or private management). considering the proposed aim in this study, it was verified for the scholars which have participated of the research, the main motivational factor that led to sport were "health" and "fitness". therewith, the conclusion is that using a research model which takes into account the interaction between characteristics of the person, the context and the time is the most efficient model to study the adolescent's disposition for sports.
Regulation of herbal medicines in Brazil: advances and perspectives
Carvalho, Ana Cecília Bezerra;Perfeito, Joo Paulo Silvério;Costa e Silva, Leandro Viana;Ramalho, Lívia Santos;Marques, Robelma France de Oliveira;Silveira, Damaris;
Brazilian Journal of Pharmaceutical Sciences , 2011, DOI: 10.1590/S1984-82502011000300004
Abstract: the national policy of integrative and complementary practices (pnpic) in the brazilian unified health system (sus), and the national policy of medicinal plants and herbal medicines (pnpmf) were launched in 2006. based on these, the brazilian health surveillance agency (anvisa) re-edited rules related to herbal medicines such as the guideline to herbal medicine registration (rdc 14/10), the good manufacture practices guideline (rdc 17/10) and the list of references to assess the safety and efficacy of herbal medicines (in 05/10). the requisites to prove herbal medicine's safety and efficacy were updated. therefore, this review aims at presenting and commenting these new rules.
Crescimento físico e estado nutricional de escolares: estudo comparativo - 1997 e 2009
Machado, Zenite;Krebs, Ruy Jornada;Prestes, Joiana Dias;Santos, Márcio Borgonovo dos;Santos, Joo Otacílio Libardoni dos;Nobre, Glauber Carvalho;Ramalho, Maria Helena da Silva;
Revista Brasileira de Cineantropometria & Desempenho Humano , 2011, DOI: 10.5007/1980-0037.2011v13n3p216
Abstract: physical growth and nutritional status are excellent health indicators since they permit the establishment of growth monitoring charts, especially for schoolchildren. the objective of this study was to compare the growth profile and nutritional status of schoolchildren between two samples (1997 and 2009). the data of physical growth and nutritional status obtained for the present sample of 645 schoolchildren (270 boys and 375 girls). the children were classified according to the body mass index (bmi)-for-age reference values of the who child growth standards. although no significant differences in height or body weight were observed between the children studied, these variables tended to increase from the first to the second sample and in the two genders. with respect to the adequacy of bmi in boys, there was an increase in the percentage of children with low bmi-for-age, doubling of the percentage of obese children, and a reduction in the percentage of children with overweight. an increase in the number of subjects with low body weight, overweight and obesity and a decrease in the number of subjects with adequate bmi-for-age were noted among girls. in conclusion, there were no significant changes in the physical growth indicators (weight, height and bmi) over the period comprising the two samples (1997 and 2009). however, height, body weight and the number of subjects with risk of obesity and obesity tended to increase, especially among girls.
Características físicas do pericarpo de sementes de milho associadas com a tolerancia à alta temperatura de secagem
José, Solange Carvalho Barrios Roveri;Von Pinho, édila Vilela de Resende;Von Pinho, Renzo Garcia;Ramalho, Magno Ant?nio Patto;Silva Filho, Joo Luis da;
Revista Brasileira de Sementes , 2005, DOI: 10.1590/S0101-31222005000100015
Abstract: differences in seed tolerance to high drying temperature have been related to the physical characteristics of the pericarp. the objective of this research was to evaluate physical aspects of the pericarp that could be associated with tolerance to high drying temperature. seeds from simple hybrids and their reciprocal simple hybrids that present differentiated behavior for tolerance by drying damage, were used. the seeds were harvested by hand on corn cobs with water content near 35% and dried at 45oc to 8% water content. the physiological quality of the seeds was evaluated through the germination test, cold test without soil and accelerated aging. twenty micron-thick sections were made with a freezing microtome at -20°c for pericarp physical assessment of the seeds. corn seeds that present denser pericarp structure, formed by more compact cells, is more sensitive to high drying temperature.
Page 1 /135367
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.