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Search Results: 1 - 10 of 773204 matches for " Jo?o Lídio da S. G. V. Junior "
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An Insight into the Transcriptome of the Digestive Tract of the Bloodsucking Bug, Rhodnius prolixus
José M. C. Ribeiro ,Fernando A. Genta,Marcos H. F. Sorgine,Raquel Logullo,Rafael D. Mesquita,Gabriela O. Paiva-Silva,David Majerowicz,Marcelo Medeiros,Leonardo Koerich,Walter R. Terra,Clélia Ferreira,André C. Pimentel,Paulo M. Bisch,Daniel C. Leite,Michelle M. P. Diniz,Joo Lídio da S. G. V. Junior,Manuela L. Da Silva,Ricardo N. Araujo,Ana Caroline P. Gandara,Sébastien Brosson,Didier Salmon,Sabrina Bousbata,Natalia González-Caballero,Ariel Mariano Silber,Michele Alves-Bezerra,Katia C. Gondim,Mário Alberto C. Silva-Neto,Georgia C. Atella,Helena Araujo,Felipe A. Dias,Carla Polycarpo,Raquel J. Vionette-Amaral,Patrícia Fampa,Ana Claudia A. Melo,Aparecida S. Tanaka,Carsten Balczun,José Henrique M. Oliveira,Renata L. S. Gon?alves,Cristiano Lazoski,Rolando Rivera-Pomar,Luis Diambra,Günter A. Schaub,Elói S. Garcia,Patrícia Azambuja,Glória R. C. Braz ,Pedro L. Oliveira
PLOS Neglected Tropical Diseases , 2014, DOI: 10.1371/journal.pntd.0002594
Abstract: The bloodsucking hemipteran Rhodnius prolixus is a vector of Chagas' disease, which affects 7–8 million people today in Latin America. In contrast to other hematophagous insects, the triatomine gut is compartmentalized into three segments that perform different functions during blood digestion. Here we report analysis of transcriptomes for each of the segments using pyrosequencing technology. Comparison of transcript frequency in digestive libraries with a whole-body library was used to evaluate expression levels. All classes of digestive enzymes were highly expressed, with a predominance of cysteine and aspartic proteinases, the latter showing a significant expansion through gene duplication. Although no protein digestion is known to occur in the anterior midgut (AM), protease transcripts were found, suggesting secretion as pro-enzymes, being possibly activated in the posterior midgut (PM). As expected, genes related to cytoskeleton, protein synthesis apparatus, protein traffic, and secretion were abundantly transcribed. Despite the absence of a chitinous peritrophic membrane in hemipterans - which have instead a lipidic perimicrovillar membrane lining over midgut epithelia - several gut-specific peritrophin transcripts were found, suggesting that these proteins perform functions other than being a structural component of the peritrophic membrane. Among immunity-related transcripts, while lysozymes and lectins were the most highly expressed, several genes belonging to the Toll pathway - found at low levels in the gut of most insects - were identified, contrasting with a low abundance of transcripts from IMD and STAT pathways. Analysis of transcripts related to lipid metabolism indicates that lipids play multiple roles, being a major energy source, a substrate for perimicrovillar membrane formation, and a source for hydrocarbons possibly to produce the wax layer of the hindgut. Transcripts related to amino acid metabolism showed an unanticipated priority for degradation of tyrosine, phenylalanine, and tryptophan. Analysis of transcripts related to signaling pathways suggested a role for MAP kinases, GTPases, and LKBP1/AMP kinases related to control of cell shape and polarity, possibly in connection with regulation of cell survival, response of pathogens and nutrients. Together, our findings present a new view of the triatomine digestive apparatus and will help us understand trypanosome interaction and allow insights into hemipteran metabolic adaptations to a blood-based diet.
Biópsia pulmonar incisional por toracoscopia paraxifoide transdiafragmática com dois portais em c?es
Basso, Paula C.;Raiser, Alceu G.;Brun, Maurício V.;Guizzo Junior,dio;Feranti, Joo P.S.;Motta, Adriana C.;Bortolini, Carlos E.;Muller, Daniel C.M.;
Pesquisa Veterinária Brasileira , 2010, DOI: 10.1590/S0100-736X2010000700010
Abstract: there are many respiratory diseases in small animals, as well as the possibilities of diagnoses, as laboratory and radiographic exams, bronchoalveolar lavage and biopsies. this research aimed at assessing the results of transdiaphragmatic paraxiphoid thoracoscopies in pulmonary biopsies of thirteen clinically healthy dogs. under general anesthesia, each dog was insufflated with 30ml kg-1 of air in each hemithorax; two trocars were placed between the xiphoid appendix and the costal arch through the diaphragm. an endoscope was introduced by the first portal; while the second portal was used to allow access to the nippers in the biopsy forceps. then, these nippers were removed and replaced by chest tube drainage. during the procedures, respiratory frequency, heart rate, oxygen saturation, arterial pressure and central venous pressure (cvp), hemogasometric parameters were checked. within 48 hours after the surgery, drains were or drain was removed, with minimal production of air and/or liquid. in conclusion, this is a fast and safe technique, which is free of trans and post surgical complications, thus allowing the collection of sufficient material for pulmonary histological evaluation.
Zoneamento bioclimático para vacas leiteiras no Estado da Bahia
Turco, Sílvia H. N.;Silva, Thieres G. F. da;Santos, Luiz F. C. dos;Ribeiro, Paulo H. B.;Araújo, Gherman G. L. de;Holanda Junior, Evandro V.;Aguiar, M?nica A.;
Engenharia Agrícola , 2006, DOI: 10.1590/S0100-69162006000100003
Abstract: daily mean meteorological data from 437 meteorological stations in the state of bahia were used to accomplish the bioclimatical zoning of milk cows and the decline in milk yield of cows with production levels of 10 and 25 kg day-1 in summer thermic condition in this state. the variables temperature (ta) and relative air humidity (rh) were used to calculate the temperature and humidity index (thi). the milk production decline (mpd) was estimated based on data of the thi and the level of production (lp). with these data the pmd and thi mapping was done for the state of bahia with the program surfer 7.0. it can be observed that through the zoning, in despite the lowest temperatures in cold months, a bioclimatical area shows great possibilities for climatical stress of milk cows. during the hottest months there are three bioclimatical zones in the state of bahia with a great occurrence of climatical stress. with the maps, areas with great probabilities for decline in milk production were observed due to unfavorable weather conditions. those areas show production losses up to 1 kg for cows with (np) of 10 kg and zones with losses as much as 4.5 kg for cows with (np) of 25 kg.
Produ??o de matéria seca e trocas gasosas em cultivares de mamoneira sob níveis de irriga??o
Freitas, Cley A. S. de;Silva, Alexandre R. A. da;Bezerra, Francisco M. L.;Lacerda, Claudivan F. de;Pereira Filho, Joo V.;Sousa, Geocleber G. de;
Revista Brasileira de Engenharia Agrícola e Ambiental , 2011, DOI: 10.1590/S1415-43662011001100009
Abstract: this study was aimed at evaluating the effects of five levels of drip irrigation in three castor bean cultivars (iac guarani, mirante 10 and brs paragua?u) by measuring the dry mass production by the different plant parts (leaf blade, petiole and plant stem) as well as by measuring the gas exchange processes (photosynthesis, stomatal conductance and transpiration). the experiment was conducted in the field, at the fazenda experimental, vale do curu, pentecoste, ceará (br). the experiment was conducted in a randomized blocks in a 5x3 factorial scheme, in split plots with three repetitions. the treatments were five irrigation levels (25, 50, 75, 100 and 125% of the class "a" pan evaporation (cae) applied to the three cultivars. the different irrigation levels influenced the variables: petiole dry mass, stomatal conductance and photosynthesis, and maximum values for these variables were obtained with irrigation level corresponding to 125% of the class a pan evaporation. there was a different response in biomass production and gas exchanges from each of the three cultivars, in all variables, except in petiole dry mass.
Antinociceptive and anti-inflammatory activity of hydroalcoholic extracts and fractions from Erythrina mulungu
Oliveira, Mariana S. G. de;Aquino, Anansa B. de;Silva, Daniel L. da;Aquino, Pedro G. V.;Santos, Mariana S.;Porfírio, Anne P. R.;Sant'Ana, Ant?nio E. G.;Santos, Bárbara V. O.;Alexandre-Moreira, Magna S.;Araújo-Júnior, Joo X. de;
Revista Brasileira de Farmacognosia , 2012, DOI: 10.1590/S0102-695X2011005000210
Abstract: erythrina mulungu mart. ex benth., fabaceae, popularly known as mulungu, is used for the treatment of insomnia and disorders of the central nervous system. this study examined the antinociceptive effects of the hydroalcoholic extracts (hae), the ethyl acetate and chloroformic fractions from e. mulungu in four experimental models of nociception using laboratory mice. the extracts and fractions were administered orally to mice at doses of 100 mg/kg. inhibition of abdominal contractions were observed for all the extracts and fractions tested, as compared to controls. all extracts and fractions from e. mulungu reduced the nociception activity produced by formalin in the 2nd phase. in the hot plate test no significant effect was observed for any extract or fraction. in the peritonitis test induced by zymosan, all of the tested extracts and the chloroformic fraction, except for the ethyl acetate phase, reduced cell migration of the peritoneal cavity. we concluded that e. mulungu shows antinociceptive effects, which are independent of the opioid system.
Antinociceptive effects of an extract, fraction and an isolated compound of the stem bark of Maytenus rigida
Martins, Marina V.;Estevam, Charles dos S.;Santos, André L. L. M.;Dias, Antonio S.;Cupertino-da-Silva, Yolanda K.;Araújo-Júnior, Joo X.;Miranda, Ana Luisa P.;Barreiro, Eliezer J.;Pizza, Cosimo;Piacente, Sonia;Montoro, Paola;Quintans-Júnior, Lucindo J.;Araujo, Brancilene S.;Alexandre-Moreira, Magna S.;Sant'Ana, Ant?nio E.G.;
Revista Brasileira de Farmacognosia , 2012, DOI: 10.1590/S0102-695X2012005000007
Abstract: the antinociceptive activity of the maytenus rigida mart. (celastraceae) ethanol extract and its ethyl acetate fraction as well as of (-)-4'-methylepigallocatechin (1), a previously isolated compound, was demonstrated in vivo. ed50 for 1 in the writhing test was 14.14 mg/kg. the acetic acid-induced writhing was inhibited by 98.4, 84.4, and 58.3%, respectively, when mice were treated with the ethanol extract, ethyl acetate fraction, and 1. in the hot plate test, mice pretreated with 1 showed significantly increased reaction times (60-89%). oral administration of 1 significantly inhibited first and second phases of the formalin-induced pain (50 and 26.5%, respectively), whereas indomethacin inhibited only the second phase of the test (41.2%). ethanol extract and its fraction showed effects on inflammatory pain, while neurogenic and inflammatory pain suppression by 1 is a strong indication of the presence of both central and peripheral effects and suggests its analgesic and anti-inflammatory potential.
Constituintes químicos das folhas de Rollinia leptopetala R. E. Fries
Costa, Vicente Carlos de O.;Tavares, Josean F.;Queiroga, Cinthia S.;Castello-Branco, Marianna V. S.;Diniz, Margareth F. F. Melo;Lima, Carolina Uch?a G. B. de;Santos, Bárbara Viviana de O.;Pita, Joo Carlos L. R.;Silva, Marcelo Sobral da;Sette, Ivana Maria Fechine;
Química Nova , 2012, DOI: 10.1590/S0100-40422012000100025
Abstract: the phytochemical investigation of rollinia leptopetala led to the isolation of a new compound named α-terpinyl caffeate, and five known compounds, being three sesquiterpenes, spathulenol, β-caryophyllene and 4β,10α-aromadendrane-diol, and two alkaloids, (-)-3-hydroxynornuciferine and (+)-norisocorydine. these alkaloids are being described for the first time in this genus. the structures of the compounds were determined by analysis of ir, ms and nmr data, as well as by comparison with literature data. the crude extract of r. leptopetala leaves demonstrated a weak cytotoxicity on sarcoma 180 cells with an ic50 of 512.3 μg/ml. however, the in vivo results showed that the extract exhibited a significant dose-dependent tumor growth reduction.
Development of a New Bio-Based Insulating Fluid from Jatropha curcas Oil for Power Transformers  [PDF]
José M. G. Evangelista Jr., Fabrício E. Bortot Coelho, Juliana A. O. Carvalho, Estêvo M. R. Araújo, Tania L. S. Miranda, Adriane Salum
Advances in Chemical Engineering and Science (ACES) , 2017, DOI: 10.4236/aces.2017.72018
Abstract: The present work aims to develop a new vegetable insulating fluid for power transformers based on Jatropha curcas oil. Besides its technical benefits, Jatropha curcas oil has a socio-economic role by promoting income to rural families, contributing to the countryside development and avoiding rural exodus. Thus, the entire transformer oil production (extraction, processing, characterization and accelerated aging) was covered and a new process was developed. For oil extraction, the most suitable process was the solvent extraction (5 mL of hexane per gram of crushed non-peeled seeds during 30 minutes) with an oil yield of 32%. In raw oil processing stage, the degumming, with 0.4 g of phosphoric acid per 100 g of oil, at 70°C, was used to remove phosphatides. Then, free fatty acids were 96% neutralized with a sodium hydroxide solution (0.5% w/w) at room temperature. For the oil clarification, the combination of 5% w/woil of activated carbon and 1% w/woil of MgO resulted in a bright, odorless and clear oil with an acid number of 0.04 mgKOH·g1. The oil drying in a vacuum rotary evaporator, at 70°C, for 2 hours reduced the water content to 177 ppm. The processed oil was characterized following ASTM D6871 methods. This oil presented higher dielectric breakdown voltage (55 kV) than commercial transformer fluids (BIOTEMP?, EnvirotempFR3?, and Bivolt?), which increases transformer safety, capacity and lifetime. In addition, the processed oil has a lower viscosity than BIOTEMP? fluid, which can enhance the heat dissipation efficiency in the transformer. Moreover, the processed oil flash and fire points of 310°C and >340°C, respectively, confirm the great security of vegetable insulating fluids. The analyzed properties of the processed oil fulfill all the ASTM D6871, ABNT NBR 15422 and IEC 62770 specifications. Therefore, Jatropha curcas oil is a potential substitute formineral insulating fluids.
Outcome of acute renal failure associated with cardiac surgery in infants
Rom?o Jr, Joo Egídio;Fuzissima, Miguel G.;Vidonho Jr, Armando F.;Noronha, Irene L.;Quintaes, Paulo Sérgio L.;Abensur, Hugo;Araújo, Maria Regina T.;Freitas Jr, Ivanir;Marcondes, Marcello;
Arquivos Brasileiros de Cardiologia , 2000, DOI: 10.1590/S0066-782X2000001000006
Abstract: objective: to analyze the impact of acute renal failure (arf) on the evolution of infants undergoing cardiac surgery. methods: we assessed 15 infants undergoing cardiac surgery who developed (arf). their demographic, clinical and surgical data, and evolution were analyzed. results: their mean age was 4.4±4.0 months (8 days to 24 months). twelve infants were males, and 4 patients already had arf at surgery. the primary cause of arf was immediate acute cardiac dysfunction in 10 infants, cardiac dysfunction associated with sepsis in 2 infants, and isolated sepsis in 3 infants. all children depended on mechanical ventilation during their postoperative period, 14 infants used vasoactive drugs, and 11 had an infectious process associated with arf. thirteen infants required dialytic treatment. eleven infants developed oluguric arf, and all had to undergo peritoneal dialysis; of the 4 patients with non-oliguric, 2 required dialysis, the main indication being hypervolemia. of these 13 dialyzed infants, 4 died in the first 24 hours because of the severity of the underlying cardiac disease (mean urea level of 49±20 mg/dl). the mortality rate for the entire group was 60% , and it was higher among the patients with oliguria arf (73% vs 25%, p<0.001). the cause of death was acute cardiac dysfunction in 6 infants (early type-1arf) and sepsis in the 3 remaining infants (late type-2 arf). conclusion: the mortality rate of arf associated with cardiac surgery in infants was hight, being higher among children with oliguria; peritoneal dialysis was indicated due to clinically uncontrolled hypervolemia and not to the uremic hypercatabolic state.
Estudo de metais e de substancias tóxicas em brinquedos
Zini, Josiane;Ferreira, Joo C.;Carvalho, Fátima M. S. de;Bustillos, José O. W.V.;Scapin, Marcos A.;Salvador, Vera L. R;Abr?o, Alcídio;
Química Nova , 2009, DOI: 10.1590/S0100-40422009000400002
Abstract: the main goal of the present study is the analysis of toxic elements in plastic toys commercialized in brazil. metals like cadmium, lead, chromium, zinc, and aluminum, along with organic substances, such as phthalates, were identified in different toys by quantitative analytical techniques. traces of thorium were detected in one of the studied samples. although the measured radioactive dose was rather low, the presence of such a radioactive contaminant is against to the international agency of atomic energy regulations. similar toys manufactured in brazil were analyzed and found to observe the standards defined by the national institute of metrology (inmetro).
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