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Search Results: 1 - 10 of 545498 matches for " Jo?o A. Pamphile "
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Co-transformation of a tropical maize endophytic isolate of Fusarium verticillioides (synonym F. moniliforme) with gusA and nia genes
Pamphile, Joo A.;Rocha, Carmen Lúcia M.S.C. da;Azevedo, Joo L.;
Genetics and Molecular Biology , 2004, DOI: 10.1590/S1415-47572004000200021
Abstract: a tropical endophytic isolate of the fungus fusarium verticillioides (synonym fusarium moniliforme) obtained from zea mays was co-transformed with plasmid pnh24 containing the fusarium oxysporum nitrate reductase nia gene and plasmid pnom 102 carrying the escherichia coli b-glucuronidase gusa gene. transformation frequency for the nia marker was 75 transformants mg-1 vector dna and introduction of the gusa gene by co-transformation was 57.2% as indicated by the presence of the gus+ phenotype on plates. southern analyses confirmed the integration of both plasmids into the genome of ten gus+ transformants. all co-transformants showed mitotic stability in respect of the gus+ phenotype. to assess the potential of transformed endophytic fungi as vectors for introducing desirable characteristics into host tropical plants of biotechnological and agricultural importance we successfully infected maize roots and detected gus+ phenotype?.
In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA
Maria Tinoco, Bárbara BA Dias, Rebeca C Dall'Astta, Joo A Pamphile, Francisco JL Arag?o
BMC Biology , 2010, DOI: 10.1186/1741-7007-8-27
Abstract: This study demonstrates for the first time the in vivo interference phenomenon in the pathogenic fungus Fusarium verticillioides, in which expression of an individual fungal transgene was specifically abolished by inoculating mycelial cells in transgenic tobacco plants engineered to express siRNAs from a dsRNA corresponding to the particular transgene.The results provide a powerful tool for further studies on molecular plant-microbe and symbiotic interactions. From a biotechnological perspective, silencing of fungal genes by generating siRNAs in the host provides a novel strategy for the development of broad fungi-resistance strategies in plants and other organisms.The genetic interference phenomenon [RNA interference (RNAi)] was described in Caenorhabditis elegans in which double-stranded RNA (dsRNA) induces individual sequence-specific posttranscriptional gene silencing. The spreading silencing effect has been demonstrated, in which the interference is observed in a broad region of the animal after the injection of dsRNA into the extracellular body cavity. Fire et al. [1] demonstrated that RNAi abolished expression of targeted genes in C. elegans by injecting dsRNA. Subsequently, others have shown that the effect also occurs when C. elegans is fed the bacterium Escherichia coli, which transcribes the recombinant dsRNA [2]. It also results from simply soaking the animals in dsRNA preparations [3]. Data obtained from in vivo and in vitro studies are now being used to engineer resistance against parasitic nematodes in transgenic plants [4-6].Cellular boundaries play a pivotal role in this integration by maintaining a level of cell autonomy while enabling communication between cells for coordinated gene expression and metabolism. In vascular plants, trafficking of RNAi molecules occurs between cells and systemically throughout the plant [7,8]. Such RNA trafficking breaks the boundaries of our traditional thinking of RNAs as functioning solely within the cells in which
Evaluation of foliar fungal endophyte diversity and colonization of medicinal plant Luehea divaricata (Martius et Zuccarini)
Bernardi-Wenzel,Juliana; García,Adriana; Filho,Celso J R; Prioli,Alberto J; Pamphile,Joo A;
Biological Research , 2010, DOI: 10.4067/S0716-97602010000400001
Abstract: endophyte microorganisms are organisms that live inside plants without causing any apparent damage to their hosts. since all plants exhibit endophyte microorganisms, it is believed that mutual association is of great importance in nature. luehea divaricata (martius & zuccarini), known popularly in brazil as agoita-cavalo, is a big-sized tree with a wide distribution in the country that possesses medicinal qualities for: dysentery, leucorrhea, rheumatism, blennorrhoea, tumors, bronchitis, and depuration. this research aims at isolating and molecularly characterizing fungi isolates from l. divaricata by sequence analysis of its1-5.8s-its2 rdna. further, the colonization of endophyte in the host plant by light and scanning electron microscopy will also be investigated. whereas, genera alternaria, cochliobolus, diaporthe, epicoccum, guignardia, phoma, and phomopsis, were identified; rdna sequence analysis revealed intra-species variability among endophyte isolates of the genus phomopsis sp. light and scanning electron microscopy techniques showed the presence of endophyte fungi inside l. divaricata leaves, inhabiting inter- and intra-cellular spaces. these types of extensive colonization and dissemination were reported throughout all the leaf parts in palisade parenchyma, esclerenchyma, spongy parenchyma, adaxial epidermis, and vascular bundle indicating colonization of endophytes in múltiple structural sub-niches in the host plant.
Genome of Herbaspirillum seropedicae Strain SmR1, a Specialized Diazotrophic Endophyte of Tropical Grasses
Fábio O. Pedrosa ,Rose Adele Monteiro,Roseli Wassem,Leonardo M. Cruz,Ricardo A. Ayub,Nelson B. Colauto,Maria Aparecida Fernandez,Maria Helena P. Fungaro,Edmundo C. Grisard,Mariangela Hungria,Humberto M. F. Madeira,Rubens O. Nodari,Clarice A. Osaku,Maria Luiza Petzl-Erler,Hernán Terenzi,Luiz G. E. Vieira,Maria Berenice R. Steffens,Vinicius A. Weiss,Luiz F. P. Pereira,Marina I. M. Almeida,Lysangela R. Alves,Anelis Marin,Luiza Maria Araujo,Eduardo Balsanelli,Valter A. Baura,Leda S. Chubatsu,Helisson Faoro,Augusto Favetti,Geraldo Friedermann,Chirlei Glienke,Susan Karp,Vanessa Kava-Cordeiro,Roberto T. Raittz,Humberto J. O. Ramos,Enilze Maria S. F. Ribeiro,Liu Un Rigo,Saul N. Rocha,Stefan Schwab,Anilda G. Silva,Eliel M. Souza,Michelle Z. Tadra-Sfeir,Rodrigo A. Torres,Audrei N. G. Dabul,Maria Albertina M. Soares,Luciano S. Gasques,Ciela C. T. Gimenes,Juliana S. Valle,Ricardo R. Ciferri,Luiz C. Correa,Norma K. Murace,Joo A. Pamphile,Eliana Valéria Patussi,Alberto J. Prioli,Sonia Maria A. Prioli,Carmem Lúcia M. S. C. Rocha,Olívia Márcia N. Arantes,Márcia Cristina Furlaneto,Leandro P. Godoy,Carlos E. C. Oliveira,Daniele Satori,Laurival A. Vilas-Boas,Maria Angélica E. Watanabe,Bibiana Paula Dambros,Miguel P. Guerra,Sandra Marisa Mathioni,Karine Louise Santos,Mario Steindel,Javier Vernal,Fernando G. Barcellos,Rubens J. Campo,Ligia Maria O. Chueire,Marisa Fabiana Nicolás,Lilian Pereira-Ferrari,José L. da Concei??o Silva,Nereida M. R. Gioppo,Vladimir P. Margarido
PLOS Genetics , 2011, DOI: 10.1371/journal.pgen.1002064
Abstract: The molecular mechanisms of plant recognition, colonization, and nutrient exchange between diazotrophic endophytes and plants are scarcely known. Herbaspirillum seropedicae is an endophytic bacterium capable of colonizing intercellular spaces of grasses such as rice and sugar cane. The genome of H. seropedicae strain SmR1 was sequenced and annotated by The Paraná State Genome Programme—GENOPAR. The genome is composed of a circular chromosome of 5,513,887 bp and contains a total of 4,804 genes. The genome sequence revealed that H. seropedicae is a highly versatile microorganism with capacity to metabolize a wide range of carbon and nitrogen sources and with possession of four distinct terminal oxidases. The genome contains a multitude of protein secretion systems, including type I, type II, type III, type V, and type VI secretion systems, and type IV pili, suggesting a high potential to interact with host plants. H. seropedicae is able to synthesize indole acetic acid as reflected by the four IAA biosynthetic pathways present. A gene coding for ACC deaminase, which may be involved in modulating the associated plant ethylene-signaling pathway, is also present. Genes for hemagglutinins/hemolysins/adhesins were found and may play a role in plant cell surface adhesion. These features may endow H. seropedicae with the ability to establish an endophytic life-style in a large number of plant species.
Pesquisa educacional com base nas artes: pensando a educa??o dos professores como experiência estética
Telles, Joo A.;
Educa??o e Pesquisa , 2006, DOI: 10.1590/S1517-97022006000300006
Abstract: the author describes in this article two studies conducted with teachers, in which art objects are used as devices to trigger shared reflection. theoretically grounded in a novel modality of qualitative investigation in the field of education - arts-based educational research (aber), the text discusses peculiarities of the working and role of this research mode in teacher professional development, the different natures of the two art objects employed (photography and theater), and points to the two main tendencies within this research mode - the tendency of meaning production, in which the teacher educator and the participants in the research share and build meanings by coming in contact with an art object previously prepared by a professional artist; and the representationist tendency, in which participating teachers and educators construct, individually or in shared fashion, a certain art object that reflects and expresses their representations of the world of teaching. lastly, the article suggests that aber, apart from establishing contexts of reflection in which students and teachers have the opportunity to unfold the aesthetic experience, institutes alternative relationships of the participants with the pedagogical knowledge and practice, revealing its social import and its renewing force.
TEACHERS? ACCOUNTS OF LANGUAGE VARIATIONS
Telles, Joo A.;
DELTA: Documenta??o de Estudos em Lingüística Teórica e Aplicada , 1998, DOI: 10.1590/S0102-44501998000100005
Abstract: this paper focuses on teachers' stigmatizing perspectives of their students' nonstandard varieties of portuguese. theoretically, the study was grounded on personal construct psychology, perspective transformation in teacher education, transformative bidialectalism and critical language awareness. the objectives were to jointly access and assess the teachers? implicit theories about nonstandard language and to verify to what extent these theories affected their classroom pedagogy. my research questions turned to how the participating teachers understood nonstandard language; and to how their understandings are reflected on their language teaching. the viewing of the teachers? videotaped classes and repertory grid activities inspired reflective conversations with the two teacher participants on their language and classroom experiences. these conversations were audio-recorded, transcribed and their analysis followed the thematizing approach of hermeneutic phenomenology. the study revealed teachers? implicit theories about language variation defining pedagogies based on deficit and standard grammar perspectives. these pedagogies are in constant interaction with the teachers? lived experiences, the contextual restrictions of schools and the limitations of teacher education programs.
Reflex o e identidade profissional do professor de LE: que histórias contam os futuros professores?
Joo A. Telles
Revista Brasileira de Linguística Aplicada , 2004,
Abstract: Este artigo relata os resultados de um estudo realizado com alunas de gradua o em Letras, Português - Língua Estrangeira (Inglês, Espanhol, Italiano, Japonês, Francês e Alem o). Objetivou verificar as metáforas, regras da prática e princípios que as alunas identificaram durante um processo de reflex o sobre suas forma es profissionais. Foi possível identificar o impacto desses elementos em seus processos individuais de desenvolvimento de identidades como professoras. O resultado foi um conjunto de histórias biográficas escritas (fontes de experiências) nas quais as participantes se baseavam para constituir suas identidades profissionais como professoras de línguas estrangeiras. Nesses processos, elas puderam construir liga es significativas entre os aspectos teóricos evidenciados nas leituras que realizaram durante o período da pesquisa e os vários eventos marcantes de suas narrativas orais acerca da carreira profissional. A associa o dos vários aspectos de suas histórias pessoais às quest es teóricas que foram discutidas durante a pesquisa revela processos de constru es de identidades profissionais como professoras de línguas estrangeiras. This paper reports on a study conducted with female pre-service teachers along the four years of their undergraduate program with major in Portuguese and a Foreign Language (English, Spanish, Italian, Japanese, French or German). The objective was to examine the metaphors, rules of practice and principles that they brought to their process of reflection upon their professional development. I have also identified the roles of these elements in their individual processes of developing their professional identity as teachers. The results were a set of written biographical stories (sources of experiences) in which the participants base themselves to constitute their professional identities as teachers of foreign languages. Within this process, they were able to construct meaningful connections between the theoretical aspects of readings that were undertaken during the research period and the many marking events of their oral narratives about their professional development. The association of the various aspects of their personal stories with the theoretical issues that were discussed during the research reveals processes of professional identity construction as teachers of foreign languages.
Os mercados, os seus saberes e as suas incertezas The Markets, Their Knowledges, and Their Uncertainties Les marchés, leur savoirs et leur incertitudes
Joo Cara?a
Revista Crítica de Ciências Sociais , 2012, DOI: 10.4000/rccs.1281
Abstract: A globaliza o, tal qual a conhecemos, n o resolve os problemas, nem equaciona as quest es, do viver em conjunto sobre a Terra. No contexto actual, a luta pela democracia assume, assim, três vectores essenciais: a reinven o do poder emancipador do conhecimento, com mais e melhores ciências, ciências sociais e outros saberes argumentativos; a reintrodu o da escola como o local privilegiado da aprendizagem para a cidadania plena; a instala o e defesa de uma sociedade do reconhecimento, em que a valoriza o do outro como interlocutor corresponda ao pulsar planetário da nossa espécie. Such as we know it, globalization does not solve the problems or address the questions of living together on the Earth. Thus, in the current context, the struggle for democracy involves three essential aspects: the reinvention of the emancipatory power of knowledge, with more and better sciences, social sciences, and other argumentative knowledges; the reintroduction of the school as the privileged site for the learning of a full citizenship; and the establishment and defense of a society of recognition, in which the valorization of the other as interlocutor corresponds to the planetary throbbing of our species. La globalisation, telle que nous la connaissons, ne résout pas les problèmes, ni met en rapport les questions, de vivre ensemble sur la planète. Dans le contexte actuel, la lutte pour la démocratie assume ainsi trois vecteurs essentiels: la réinvention du pouvoir émancipateur de la connaissance, grace à plus de sciences et de meilleures sciences, sciences sociales et d’autres savoirs argumentatifs; la réintroduction de l’école comme lieu privilégié de l’apprentissage pour la pleine citoyenneté; l’instauration et la défense d’une société de reconnaissance, dans laquelle la valorisation de l’autre en tant qu’interlocuteur corresponde à la pulsation planétaire de notre espèce.
Evolu o versus cria o: falso dilema = Evolution versus creation: a false dilemma
MacDowell, Joo A.
Veritas , 2011,
Abstract: A evolu o cósmica e biológica é hoje um fato universalmente admitido pela comunidade científica. Muitos julgam que essa posi o científica é incompatível com a aceita o de um Deus criador do mundo, seja que neguem a cria o em nome da evolu o, seja que rejeitem a evolu o em nome da fé. O artigo pretende apontar a inconsistência do dilema “evolu o ou cria o”, mostrando, mediante uma reflex o filosófica, que ele deriva de uma falsa compreens o do método e do alcance tanto das ciências positivas como da teologia. Defende, além disso, que compete aos cientistas verificar se o neodarwinismo da teoria sintética é ou n o uma explica o científica satisfatória dos fen menos. Afirma, porém, que também essa teoria seria compatível com uma ideia adequada de cria o, mesmo que n o recorra, para explicar a evolu o, nem à interven o de uma causa eficiente trans-empírica, nem a uma inteligência superior que oriente o processo evolutivo para determinados fins. Com efeito, a a o criadora de Deus que confere o ser e agir a toda a realidade natural e a orienta, de algum modo, para seus fins, tem um caráter transcendente, e, por princípio, n o pode ser detectada pela investiga o científica, mas, t o somente, pela reflex o filosófica. Cosmic and biological evolution is universally accepted by the scientific community today. Many people, however, believe that this scientific position is irreconcilable with the idea of a world-creator God; they either deny creation in virtue of evolution or reject evolution in virtue of their faith. This paper does not intend to prove God’s existence. Its purpose is to show the fallacy of the evolution/creation dilemma and to explain by means of philosophical reflection that this dilemma arises from a false understanding of the respective methods and purviews of the positive sciences and theology. Moreover, the paper maintains that it falls to scientific research to establish whether the neo-darwinian theory of evolution provides a satisfactory explanation of the phenomena or not. It claims nevertheless that such a theory, should it be definitively confirmed, could also be compatible with an appropriate understanding of creation, even if its explanation of evolution does not appeal to the intervention of an efficient trans-empirical cause nor to a higher intelligence which would guide the evolutionary process towards its goals. In fact, if God exists, then God’s creative action, which gives being and act to the whole natural reality and guides it towards its ultimate ends, has a transcendent character and by no means can it be detec
TEACHERS? ACCOUNTS OF LANGUAGE VARIATIONS
Telles Joo A.
DELTA: Documenta??o de Estudos em Lingüística Teórica e Aplicada , 1998,
Abstract: This paper focuses on teachers' stigmatizing perspectives of their students' nonstandard varieties of Portuguese. Theoretically, the study was grounded on Personal Construct Psychology, Perspective Transformation in Teacher Education, Transformative Bidialectalism and Critical Language Awareness. The objectives were to jointly access and assess the teachers? implicit theories about nonstandard language and to verify to what extent these theories affected their classroom pedagogy. My research questions turned to how the participating teachers understood nonstandard language; and to how their understandings are reflected on their language teaching. The viewing of the teachers? videotaped classes and Repertory Grid activities inspired reflective conversations with the two teacher participants on their language and classroom experiences. These conversations were audio-recorded, transcribed and their analysis followed the thematizing approach of Hermeneutic Phenomenology. The study revealed teachers? implicit theories about language variation defining pedagogies based on deficit and standard grammar perspectives. These pedagogies are in constant interaction with the teachers? lived experiences, the contextual restrictions of schools and the limitations of teacher education programs.
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