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Search Results: 1 - 10 of 73723 matches for " Jing-Jiang Zhou "
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Identification and expression profiling of putative odorant-binding proteins in the malaria mosquitoes, Anopheles gambiae and A. arabiensis
Zhengxi Li,Jing-Jiang Zhou,Zuorui Shen,Field Lin
Science China Life Sciences , 2004, DOI: 10.1360/03yc0232
Abstract: Olfaction plays a major role in host-seeking behaviour of mosquitoes. An informatics-based genome-wide analysis of odorant-binding protein (OBP) homologues is undertaken, and 32 putative OBP genes in total in the whole genome sequences of Anopheles gambiae are identified. Tissue-specific expression patterns of all A. gambiae OBP candidates are determined by semi-quantitative Reverse Transcription (RT)-PCR using mosquito actin gene as internal expression control standard. The results showed that 20 OBP candidates had strong expression in mosquito olfactory tissues (female antennae), which indicate that OBPs may play an important role in regulating mosquito olfactory behaviours. Species-specific expression patterns of all putative anopheline OBPs are also studied in two of the most important malaria vectors in A. gambiae complex, i.e. A. gambiae and A. arabiensis, which found 12 of the putative OBP genes examined displayed species-differential expression patterns. The cumulative relative expression intensity of the OBPs in A. arabiensis antennae was higher than that in A. gambiae (the ratio is 1441.45:1314.12), which might be due to their different host preference behaviour. While A. gambiae is a highly anthropophilic mosquito, A. arabiensis is more opportunistic (varying from anthropophilic to zoophilic). So the latter should need more OBPs to support its host selection preference. Identification of mosquito OBPs and verification of their tissue- and species-specific expression patterns represent the first step towards further molecular analysis of mosquito olfactory mechanism, such as recombinant expression and ligand identification.
Unique Features of Odorant-Binding Proteins of the Parasitoid Wasp Nasonia vitripennis Revealed by Genome Annotation and Comparative Analyses
Filipe G. Vieira, Sylvain Forêt, Xiaoli He, Julio Rozas, Linda M. Field, Jing-Jiang Zhou
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0043034
Abstract: Insects are the most diverse group of animals on the planet, comprising over 90% of all metazoan life forms, and have adapted to a wide diversity of ecosystems in nearly all environments. They have evolved highly sensitive chemical senses that are central to their interaction with their environment and to communication between individuals. Understanding the molecular bases of insect olfaction is therefore of great importance from both a basic and applied perspective. Odorant binding proteins (OBPs) are some of most abundant proteins found in insect olfactory organs, where they are the first component of the olfactory transduction cascade, carrying odorant molecules to the olfactory receptors. We carried out a search for OBPs in the genome of the parasitoid wasp Nasonia vitripennis and identified 90 sequences encoding putative OBPs. This is the largest OBP family so far reported in insects. We report unique features of the N. vitripennis OBPs, including the presence and evolutionary origin of a new subfamily of double-domain OBPs (consisting of two concatenated OBP domains), the loss of conserved cysteine residues and the expression of pseudogenes. This study also demonstrates the extremely dynamic evolution of the insect OBP family: (i) the number of different OBPs can vary greatly between species; (ii) the sequences are highly diverse, sometimes as a result of positive selection pressure with even the canonical cysteines being lost; (iii) new lineage specific domain arrangements can arise, such as the double domain OBP subfamily of wasps and mosquitoes.
Plant Volatile Analogues Strengthen Attractiveness to Insect
Yufeng Sun, Hao Yu, Jing-Jiang Zhou, John A. Pickett, Kongming Wu
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0099142
Abstract: Green leaf bug Apolygus lucorum (Meyer-Dür) is one of the major pests in agriculture. Management of A. lucorum was largely achieved by using pesticides. However, the increasing population of A. lucorum since growing Bt cotton widely and the increased awareness of ecoenvironment and agricultural product safety makes their population-control very challenging. Therefore this study was conducted to explore a novel ecological approach, synthetic plant volatile analogues, to manage the pest. Here, plant volatile analogues were first designed and synthesized by combining the bioactive components of β-ionone and benzaldehyde. The stabilities of β-ionone, benzaldehyde and analogue 3 g were tested. The electroantennogram (EAG) responses of A. lucorum adult antennae to the analogues were recorded. And the behavior assay and filed experiment were also conducted. In this study, thirteen analogues were acquired. The analogue 3 g was demonstrated to be more stable than β-ionone and benzaldehyde in the environment. Many of the analogues elicited EAG responses, and the EAG response values to 3 g remained unchanged during seven-day period. 3 g was also demonstrated to be attractive to A. lucorum adults in the laboratory behavior experiment and in the field. Its attractiveness persisted longer than β-ionone and benzaldehyde. This indicated that 3 g can strengthen attractiveness to insect and has potential as an attractant. Our results suggest that synthetic plant volatile analogues can strengthen attractiveness to insect. This is the first published study about synthetic plant volatile analogues that have the potential to be used in pest control. Our results will support a new ecological approach to pest control and it will be helpful to ecoenvironment and agricultural product safety.
The Dissociation of Egocentric and Allocentric Mental Rotation through an In- Rotation Effect

TAO Wei-Dong,SUN Hong-Jin,YAN Jing-Jiang,ZHOU Liu,

心理学报 , 2008,
Abstract: 采用虚拟的旋转不同角度左、右手模型,构建"左右手判断(Left and right hand judgment:LR)"任务和"相同-不同判断(same and different judgment:SD)"任务,考察这两种实验任务是否都存在内旋效应和角度效应,以此推论被试采用何种旋转策略.结果发现:(1)两种实验任务结果均表现出显著的角度效应;(2)在LR任务条件下,存在显著的内旋效应,而在SD任务中不存在内旋效应.从而表明当人手图片作为心理旋转材料时,它具有双重角色.被试心理旋转加工时究竞选用何种参照系的旋转策略,与实验材料和实验任务两者都密不可分.

LIU Jing-jiang,LIU Wen-zhong,ZHOU Hua-rong,HOU Chun-rong,

高分子学报 , 1989,
Abstract: The dynamic mechanical properties and morphology of binary copolymer networks (BCN's) of vinyl-terminated polyurethane (VTPU) prepolymer and vinyl monomers such as styrene(St), methyl methacrylate(MMA), vinyl acetate(VAc) and butyl methacrylate(BMA) were studied. All the BCN's samples have semicompatible feature on the dynamic mechanical spectroscopy. The compatibility between components increases with the increasing cross-link density due to the increase in the entropy of mixing. The amount of soluble species varies with composition of BCN's. The sol fraction can improve the compatibility between components.
Sniffing Out Chemosensory Genes from the Mediterranean Fruit Fly, Ceratitis capitata
Paolo Siciliano, Francesca Scolari, Ludvik M. Gomulski, Marco Falchetto, Mosè Manni, Paolo Gabrieli, Linda M. Field, Jing-Jiang Zhou, Giuliano Gasperi, Anna R. Malacrida
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0085523
Abstract: The Mediterranean fruit fly, Ceratitis capitata (medfly), is an extremely invasive agricultural pest due to its extremely wide host range and its ability to adapt to a broad range of climatic conditions and habitats. Chemosensory behaviour plays an important role in many crucial stages in the life of this insect, such as the detection of pheromone cues during mate pursuit and odorants during host plant localisation. Thus, the analysis of the chemosensory gene repertoire is an important step for the interpretation of the biology of this species and consequently its invasive potential. Moreover, these genes may represent ideal targets for the development of novel, effective control methods and pest population monitoring systems. Expressed sequence tag libraries from C. capitata adult heads, embryos, male accessory glands and testes were screened for sequences encoding putative odorant binding proteins (OBPs). A total of seventeen putative OBP transcripts were identified, corresponding to 13 Classic, three Minus-C and one Plus-C subfamily OBPs. The tissue distributions of the OBP transcripts were assessed by RT-PCR and a subset of five genes with predicted proteins sharing high sequence similarities and close phylogenetic affinities to Drosophila melanogaster pheromone binding protein related proteins (PBPRPs) were characterised in greater detail. Real Time quantitative PCR was used to assess the effects of maturation, mating and time of day on the transcript abundances of the putative PBPRP genes in the principal olfactory organs, the antennae, in males and females. The results of the present study have facilitated the annotation of OBP genes in the recently released medfly genome sequence and represent a significant contribution to the characterisation of the medfly chemosensory repertoire. The identification of these medfly OBPs/PBPRPs permitted evolutionary and functional comparisons with homologous sequences from other tephritids of the genera Bactrocera and Rhagoletis.
Differential Expression Patterns in Chemosensory and Non-Chemosensory Tissues of Putative Chemosensory Genes Identified by Transcriptome Analysis of Insect Pest the Purple Stem Borer Sesamia inferens (Walker)
Ya-Nan Zhang, Jun-Yan Jin, Rong Jin, Yi-Han Xia, Jing-Jiang Zhou, Jian-Yu Deng, Shuang-Lin Dong
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0069715
Abstract: Background A large number of insect chemosensory genes from different gene subfamilies have been identified and annotated, but their functional diversity and complexity are largely unknown. A systemic examination of expression patterns in chemosensory organs could provide important information. Methodology/Principal Findings We identified 92 putative chemosensory genes by analysing the transcriptome of the antennae and female sex pheromone gland of the purple stem borer Sesamia inferens, among them 87 are novel in this species, including 24 transcripts encoding for odorant binding proteins (OBPs), 24 for chemosensory proteins (CSPs), 2 for sensory neuron membrane proteins (SNMPs), 39 for odorant receptors (ORs) and 3 for ionotropic receptors (IRs). The transcriptome analyses were validated and quantified with a detailed global expression profiling by Reverse Transcription-PCR for all 92 transcripts and by Quantitative Real Time RT-PCR for selected 16 ones. Among the chemosensory gene subfamilies, CSP transcripts are most widely and evenly expressed in different tissues and stages, OBP transcripts showed a clear antenna bias and most of OR transcripts are only detected in adult antennae. Our results also revealed that some OR transcripts, such as the transcripts of SNMP2 and 2 IRs were expressed in non-chemosensory tissues, and some CSP transcripts were antenna-biased expression. Furthermore, no chemosensory transcript is specific to female sex pheromone gland and very few are found in the heads. Conclusion Our study revealed that there are a large number of chemosensory genes expressed in S. inferens, and some of them displayed unusual expression profile in non-chemosensory tissues. The identification of a large set of putative chemosensory genes of each subfamily from a single insect species, together with their different expression profiles provide further information in understanding the functions of these chemosensory genes in S. inferens as well as other insects.
Identification and Expression Profiling of Odorant Binding Proteins and Chemosensory Proteins between Two Wingless Morphs and a Winged Morph of the Cotton Aphid Aphis gossypii Glover
Shao-Hua Gu, Kong-Ming Wu, Yu-Yuan Guo, Linda M. Field, John A. Pickett, Yong-Jun Zhang, Jing-Jiang Zhou
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0073524
Abstract: Insects interact with their environment and respond to the changes in host plant conditions using semiochemicals. Such ecological interactions are facilitated by the olfactory sensilla and the use of olfactory recognition proteins. The cotton aphid Aphis gossypii can change its phenotype in response to ecological conditions. They reproduce mainly as wingless asexual morphs but develop wings to find mates or new plant hosts under the influence of environmental factors such as temperature, plant nutrition and population density. Two groups of small soluble proteins, odorant binding proteins (OBPs) and chemosensory proteins (CSPs) are believed to be involved in the initial biochemical recognition steps in semiochemical perception. However, the exact molecular roles that these proteins play in insect olfaction remain to be discovered. In this study, we compared the transcriptomes of three asexual developmental stages (wingless spring and summer morphs and winged adults) and characterised 9 OBP and 9 CSP genes. The gene structure analysis showed that the number and length of introns in these genes are much higher and this appears to be unique feature of aphid OBP and CSP genes in general. Another unique feature in aphids is a higher abundance of CSP transcripts than OBP transcripts, suggesting an important role of CSPs in aphid physiology and ecology. We showed that some of the transcripts are overexpressed in the antennae in comparison to the bodies and highly expressed in the winged aphids compared to wingless morphs, suggesting a role in host location. We examined the differential expression of these olfactory genes in ten aphid species and compared the expression profile with the RNA-seq analyses of 25 pea aphid transcriptome libraries hosted on AphidBase.
Functional Characterizations of Chemosensory Proteins of the Alfalfa Plant Bug Adelphocoris lineolatus Indicate Their Involvement in Host Recognition
Shao-Hua Gu, Song-Ying Wang, Xue-Ying Zhang, Ping Ji, Jing-Tao Liu, Gui-Rong Wang, Kong-Ming Wu, Yu-Yuan Guo, Jing-Jiang Zhou, Yong-Jun Zhang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0042871
Abstract: Insect chemosensory proteins (CSPs) have been proposed to capture and transport hydrophobic chemicals from air to olfactory receptors in the lymph of antennal chemosensilla. They may represent a new class of soluble carrier protein involved in insect chemoreception. However, their specific functional roles in insect chemoreception have not been fully elucidated. In this study, we report for the first time three novel CSP genes (AlinCSP1-3) of the alfalfa plant bug Adelphocoris lineolatus (Goeze) by screening the antennal cDNA library. The qRT-PCR examinations of the transcript levels revealed that all three genes (AlinCSP1-3) are mainly expressed in the antennae. Interestingly, these CSP genes AlinCSP1-3 are also highly expressed in the 5th instar nymphs, suggesting a proposed function of these CSP proteins (AlinCSP1-3) in the olfactory reception and in maintaining particular life activities into the adult stage. Using bacterial expression system, the three CSP proteins were expressed and purified. For the first time we characterized the types of sensilla in the antennae of the plant bug using scanning electron microscopy (SEM). Immunocytochemistry analysis indicated that the CSP proteins were expressed in the pheromone-sensitive sensilla trichodea and general odorant-sensitive sensilla basiconica, providing further evidence of their involvement in chemoreception. The antennal activity of 55 host-related semiochemicals and sex pheromone compounds in the host location and mate selection behavior of A. lineolatus was investigated using electroantennogram (EAG), and the binding affinities of these chemicals to the three CSPs (AlinCSP1-3) were measured using fluorescent binding assays. The results showed several host-related semiochemicals, (Z)-3-hexen-1-ol, (E)-2-hexen-1-al and valeraldehyde, have a high binding affinity with AlinCSP1-3 and can elicit significant high EAG responses of A. lineolatus antennae. Our studies indicate the three antennae-biased CSPs may mediate host recognition in the alfalfa plant bug A. lineolatus.
Hypoxic-ischemic injury decreases anxiety-like behavior in rats when associated with loss of tyrosine-hydroxylase immunoreactive neurons of the substantia nigra
Ming-Yan, Hei;Luo, Ya-Li;Zhang, Xiao-Chun;Liu, Hong;Gao, Ru;Wu, Jing-Jiang;
Brazilian Journal of Medical and Biological Research , 2012, DOI: 10.1590/S0100-879X2011007500161
Abstract: neonatal sprague-dawley rats were randomly divided into normal control, mild hypoxia-ischemia (hi), and severe hi groups (n = 10 in each group at each time) on postnatal day 7 (p7) to study the effect of mild and severe hi on anxiety-like behavior and the expression of tyrosine hydroxylase (th) in the substantia nigra (sn). the mild and severe hi groups were exposed to hypoxia (8% o2/92% n2) for 90 and 150 min, respectively. the elevated plus-maze (epm) test was performed to assess anxiety-like behavior by measuring time spent in the open arms (oat) and oat%, and immunohistochemistry was used to determine the expression of th in the sn at p14, p21, and p28. oat and oat% in the epm were significantly increased in both the mild (1.88-, 1.99-, and 2.04-fold, and 1.94-, 1.51-, and 1.46-fold) and severe hi groups (1.69-, 1.68-, and 1.87-fold, and 1.83-, 1.43-, and 1.39-fold, respectively; p < 0.05). the percent of th-positive cells occupying the sn area was significantly and similarly decreased in both the mild (17.7, 40.2, and 47.2%) and severe hi groups (16.3, 32.2, and 43.8%, respectively; p < 0.05). the decrease in the number of th-positive cells in the sn and the level of protein expression were closely associated (pearson correlation analysis: r = 0.991, p = 0.000 in the mild hi group and r = 0.974, p = 0.000 in the severe hi group) with the impaired anxiety-like behaviors. we conclude that neonatal hi results in decreased anxiety-like behavior during the juvenile period of sprague-dawley rats, which is associated with the decreased activity of th in the sn. the impairment of anxiety and the expression of th are not likely to be dependent on the severity of hi.
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