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Search Results: 1 - 10 of 80920 matches for " JinYuan Liu "
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Isolation of the promoter of a cotton β-galactosidase gene (GhGal1) and its expression in transgenic tobacco plants
Aimin Wu,Jinyuan Liu
Science China Life Sciences , 2006, DOI: 10.1007/s11427-006-0105-7
Abstract: β-galactosidases (EC 3.2.1.23) constitute a widespread family of glycosyl hydrolases in plants and are thought to be involved in metabolism of cell wall polysaccharides. A cDNA of the cotton (Gossypium hirsutum) β-galactosidase gene, designated GhGal1, has previously been identified and its transcripts are highly abundant at the elongation stage of the cotton fiber. To examine the temporal and spatial control of GhGal1 expression, a transcriptional fusion of the GhGal1 promoter region (1770 bp) with the β-glucuronidase (GUS) reporter gene was introduced into tobacco plants by the Agrobacterium infection method. The resulting transgenic plants showed higher GUS activity of fruit in the transgenic plants than that in the negative and positive controls. Histochemical localization of GUS activity demonstrated that the expression of the GUS gene could be found in the meristem zones of roots, cotyledons, vascular tissues, fruit and trichomes in transgenic tobacco plants. Additionally, sequence analysis of the regulatory region also revealed several conserved motifs among which some were shared with previously reported fruit/seed-specific elements and the others were related with trichome expression. These results indicated the temporal and spatial expression characterization of the GhGal1 promoter in transgenic tobacco plants and provided an important insight into the roles of GhGal1 in cotton fiber development.
Molecular cloning and characterization of a cotton phosphoenolpyruvate carboxylase gene

Zhixin Qiao,Jinyuan Liu,

自然科学进展 , 2008,
Abstract: Phosphoenolpyruvate carboxylase (PEPC) plays diverse physiological functions during plant development. In this study, a new phosphoenolpyruvate carboxylase gene GhPEPC2 is isolated from cotton (Gossypium hirsutum cv. zhongmian 35) by RACE-PCR. The cloned cDNA of GhPEPC2 is 3364 bp in length, and has an open reading frame of 2913 bp, encoding for 971 putative amino acids with a calculated molecular mass of 110.6 kD and pI of 5.56. The deduced amino acid sequence of GhPEPC2 shares high similarity with other reported plant PEPCs. Southern blot analysis indicates that the cotton PEPC exists as a small gene family and the GhPEPC2 might have two copies in the cotton genome. The semi-quantitative RT-PCR reveals that GhPEPC2 constitutively expresses in all the tissues of cotton and accumulated highly in roots, flowers and embryos but relatively low in stems and fibers. In addition, the recombinant GhPEPC2 has been purified by expressing it in Escherichia coli and the catalytic properties of it were also investigated. The results showed that GhPEPC2 is a typical C3 PEPC with a higher Km (83.6 μM) and lower Vmax (8.0 μmol min 1 mg 1) compared with the C3 PEPCs previously reported.
Cloning and characterization of cotton heteromeric acetyl-CoA carboxylase genes

Qiao Zhixin,Liu Jinyuan,

自然科学进展 , 2007,
Abstract:
Cosmic-ray exposure and gas retention ages of the Guangmingshan (H5) chondrite
Daode Wang,Yangting Lin,Jinyuan Liu
Chinese Science Bulletin , 2001, DOI: 10.1007/BF02900577
Abstract: Isotopic compositions of noble gases from the Guangmingshan chondrite were analyzed. Based on the analyses of cosmogenic nuclei, cosmic-ray exposure age of the meteorite is (65±10.0) Ma (3He), (80±12) Ma (21Ne) and (65±10.0) Ma (38Ar), with an average of 70 Ma. This is the highest exposure age of H-group ordinary chondrites. Gas retention ages of K-Ar and U, Th-4He are (4230±100) Ma and (3300±60) Ma, respectively. The smaller ages of3He than21Ne and4He than40Ar suggest that both3He and4He lost together. This is probably related to a solar heating effect of a meteorite with a small perihelion during the last exposure period.
Molecular cloning and functional characterization of a DREB1/CBF-like gene (GhDREB1L) from cotton
Bo Huang,LongGuo Jin,JinYuan Liu
Science China Life Sciences , 2007, DOI: 10.1007/s11427-007-0010-8
Abstract: The transcription factors DREB1s/CBFs play important roles in the regulation of plant resistance to environmental stresses and are quite useful for generating transgenic plants tolerant to these stresses. In the present work, a cDNA encoding DREB1/CBF-like protein (GhDREB1L) from cotton was isolated, and its sequence features, DNA binding preference, and expression patterns of the transcripts were also characterized. GhDREB1L contained one conserved AP2/ERF domain and its amino acid sequence was similar to the DREB1/CBF group of the DREB family from other plants. The DNA-binding domain of GhDREB1L was successfully expressed as a fusion protein in Escherichia coli BL21 (DE3) and purified by Ni-NTA affinity chromatography. Electrophoretic mobility shift assay revealed that the purified GhDREB1L fusion protein had a specific binding activity with the previously characterized DRE element (core sequence, ACCGAC) and also with the DRE-like sequence (core sequence, GCCGAC) in the promoter of the dehydration-responsive late embryogenesis-abundant gene LEA D113. Semi-quantitative RT-PCR showed that GhDREB1L was induced in the cotton cotyledons by low temperature, as well as drought and NaCl treatments. These results suggested that the novel cotton GhDREB1L might play an important role in response to low temperature as well as drought and high salinity through binding to the DRE cis-element.
Modeling of kiwifruit metallothionein kiwi503
Chunming Zhu,Tun Lü,Riqing Zhang,Nanming Zhao,Jinyuan Liu
Chinese Science Bulletin , 2000, DOI: 10.1007/BF02886249
Abstract: Kiwifruit metallothionein, kiwi503, is a typical plant metallothionein protein. It has 63 amino acid residues in two cysteine-rich regions and one spacer region of about 32 residues. In this note, the two cysteine-rich regions and the spacer region have been modeted separatety by the distance geometry and the homology method. The three parts are then connected to generate a three-dimensional structural modet of kiwifruit metallothionein kiwi503. The result shows that there is no structural or energy barrier preventing either cysteine rich domain from independently forming a metal-sulfur chetating cluster. The method can also be applied to predicting the structures of the same type of other proteins.
Cloning and sequence characteristics of the genomic gene of a rice metallothionein
Lihua Yu,Jinyuan Liu,Umeda Masaaki,Uchimiya Hirofumi,Nanming Zhao
Chinese Science Bulletin , 2000, DOI: 10.1007/BF02884663
Abstract: Northern blot analysis showed that a metallothionein gene,ricMT, is expressed strongly in the stem of rice with an expression level that could be more than 100-fold stronger than in leaf blades. The results suggest that the 5′ upstream region flanking the coding sequence of thericMT may contain a fairly strong promoter. To elucidate its regulation and promoter structure, the genomic clones ofricMT were screened out from a rice genomic library and a fragment of about 4 084 bp was sequenced. The fragment included a 5′ upstream region of ca. 2 970 bp, a transcription region of ca. 690 bp and a 3′ downstream region of ca. 420 bp. Computer analysis of the sequence homology showed that the 5′ upstream region included a putative TATA box, a putative CAAT box, and a typical metal-responsive element TGCGCGCG. The results will promote further understanding of the mechanisms of gene regulation and metal response of plant metallothionein proteins.
Expression patterns of the rice class I metallothionein gene family in response to lead stress in rice seedlings and functional complementation of its members in lead-sensitive yeast cells
YuFeng Xu,GongKe Zhou,Lu Zhou,YiQin Li,JinYuan Liu
Chinese Science Bulletin , 2007, DOI: 10.1007/s11434-007-0335-5
Abstract: Metallothioneins (MTs) are a group of low molecular mass and cysteine-rich proteins that can chelate heavy-metal ions. In this paper, Northern blot analysis was used to investigate the influence of lead stress on the expression patterns of 10 rice class I MT genes (OsMT-Is) in rice seedlings. With the exception of OsMT-I-3b, the data demonstrate dynamic changes of 9 OsMT-I transcripts in response to Pb2+ treatment in rice seedling roots. Of these genes, transcription of OsMT-I-1a, OsMT-I-1b, OsMT-I-2c, OsMT-I-4a, OSMT-I-4b and OsMT-I-4c increased significantly, while transcription of OsMT-I-2a and OsMT-I-3a increased marginally. In contrast, the expression of OsMT-I-2b was inhibited. Pb2+ induced the expression of 6 OsMT-I genes in seedling shoots, but had no obvious effects on the expression of OsMT-I-1a, OsMT-I-1b, OsMT-I-4a and OsMT-I-4b. All the 10 OsMT-Is had enhanced lead tolerance when heterologously expressed in lead-sensitive yeast mutant cells. These results provide an expression profile of the rice MT gene family in response to Pb2+ stress in rice seedlings and demonstrate increased lead tolerance in sensitive yeast mutant cells expressing OsMT-Is. This study lays a foundation for further analysis of the role of the rice MT gene family in respond to Pb2+ stress.
Zinc up-regulated the expression of the rice metallonthionein gene family and enhanced the zinc tolerance of yeast cells

Xu Yufeng,Zhou Gongke,Li Yiqin,Liu Jinyuan,

自然科学进展 , 2007,
Abstract:
Effects of V2O5 Addition on Microwave Dielectric Properties of Li2ZnTi3O8 Ceramics for LTCC Applications  [PDF]
Jianhua Zhu, Jinyuan Liu, Songjie Lu, Yong Zeng
New Journal of Glass and Ceramics (NJGC) , 2019, DOI: 10.4236/njgc.2019.93003
Abstract: The sintering temperature of Li2ZnTi3O8 ceramics is still high for LTCC-based applications. In this work, V2O5 was doped as the sintering aid. The sintered density, phase composition, grain size, as well as microwave dielectric properties of Li2ZnTi3O8 ceramics with the addition of V2O5 were investigated. Based on our research, V2O5 doping effectively promoted the densification of Li2ZnTi3O8 ceramics at about 900°C, without affecting the main crystal phase of the ceramics. Li2ZnTi3O8 ceramics with 0.5 wt% V2O5
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