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Search Results: 1 - 10 of 502972 matches for " Jin-A Kim "
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Anti-inflammatory and anti-amyloidogenic effects of a small molecule, 2,4-bis(p-hydroxyphenyl)-2-butenal in Tg2576 Alzheimer’s disease mice model
Jin Peng,Kim Jin-A,Choi Dong-Young,Lee Young-Jung
Journal of Neuroinflammation , 2013, DOI: 10.1186/1742-2094-10-2
Abstract: Background Alzheimer’s disease (AD) is pathologically characterized by excessive accumulation of amyloid-beta (Aβ) fibrils within the brain and activation of astrocytes and microglial cells. In this study, we examined anti-inflammatory and anti-amyloidogenic effects of 2,4-bis(p-hydroxyphenyl)-2-butenal (HPB242), an anti-inflammatory compound produced by the tyrosine-fructose Maillard reaction. Methods 12-month-old Tg2576 mice were treated with HPB242 (5 mg/kg) for 1 month and then cognitive function was assessed by the Morris water maze test and passive avoidance test. In addition, western blot analysis, Gel electromobility shift assay, immunostaining, immunofluorescence staining, ELISA and enzyme activity assays were used to examine the degree of Aβ deposition in the brains of Tg2576 mice. The Morris water maze task was analyzed using two-way ANOVA with repeated measures. Otherwise were analyzed by one-way ANOVA followed by Dunnett’s post hoc test. Results Treatment of HPB242 (5 mg/kg for 1 month) significantly attenuated cognitive impairments in Tg2576 transgenic mice. HPB242 also prevented amyloidogenesis in Tg2576 transgenic mice brains. This can be evidenced by Aβ accumulation, BACE1, APP and C99 expression and β-secretase activity. In addition, HPB242 suppresses the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) as well as activation of astrocytes and microglial cells. Furthermore, activation of nuclear factor-kappaB (NF-κB) and signal transducer and activator of transcription 1/3 (STAT1/3) in the brain was potently inhibited by HPB242. Conclusions Thus, these results suggest that HPB242 might be useful to intervene in development or progression of neurodegeneration in AD through its anti-inflammatory and anti-amyloidogenic effects.
Concentrations of TSP-Bound Metals in Four Urban Residential Locations in Seoul, Korea
Hang Thi Nguyen,Chan Goo Park,Jin-A Kim,Je-Seung Lee
The Scientific World Journal , 2010, DOI: 10.1100/tsw.2010.70
The Korea Brassica Genome Project: a Glimpse of the Brassica Genome Based on Comparative Genome Analysis With Arabidopsis
Tae-Jin Yang,Jung-Sun Kim,Ki-Byung Lim,Soo-Jin Kwon,Jin-A Kim,Mina Jin,Jee Young Park,Myung-Ho Lim,Ho-Il Kim,Seog Hyung Kim,Yong Pyo Lim,Beom-Seok Park
Comparative and Functional Genomics , 2005, DOI: 10.1002/cfg.465
Abstract: A complete genome sequence provides unlimited information in the sequenced organism as well as in related taxa. According to the guidance of the Multinational Brassica Genome Project (MBGP), the Korea Brassica Genome Project (KBGP) is sequencing chromosome 1 (cytogenetically oriented chromosome #1) of Brassica rapa. We have selected 48 seed BACs on chromosome 1 using EST genetic markers and FISH analyses. Among them, 30 BAC clones have been sequenced and 18 are on the way. Comparative genome analyses of the EST sequences and sequenced BAC clones from Brassica chromosome 1 revealed their homeologous partner regions on the Arabidopsis genome and a syntenic comparative map between Brassica chromosome 1 and Arabidopsis chromosomes. In silico chromosome walking and clone validation have been successfully applied to extending sequence contigs based on the comparative map and BAC end sequences. In addition, we have defined the (peri)centromeric heterochromatin blocks with centromeric tandem repeats, rDNA and centromeric retrotransposons. In-depth sequence analyses of five homeologous BAC clones and an Arabidopsis chromosomal region reveal overall co-linearity, with 82% sequence similarity. The data indicate that the Brassica genome has undergone triplication and subsequent gene losses after the divergence of Arabidopsis and Brassica. Based on in-depth comparative genome analyses, we propose a comparative genomics approach for conquering the Brassica genome. In 2005 we intend to construct an integrated physical map, including sequence information from 500 BAC clones and integration of fingerprinting data and end sequence data of more than 100 000 BAC clones. The sequences have been submitted to GenBank with accession numbers: 10 204 BAC ends of the KBrH library (CW978640–CW988843); KBrH138P04, AC155338; KBrH117N09, AC155337; KBrH097M21, AC155348; KBrH093K03, AC155347; KBrH081N08, AC155346; KBrH080L24, AC155345; KBrH077A05, AC155343; KBrH020D15, AC155340; KBrH015H17, AC155339; KBrH001H24, AC155335; KBrH080A08, AC155344; KBrH004D11, AC155341; KBrH117M18, AC146875; KBrH052O08, AC155342.
Flavonoids Isolated from Korea Citrus aurantium L. Induce G2/M Phase Arrest and Apoptosis in Human Gastric Cancer AGS Cells
Do-Hoon Lee,Kwang-Il Park,Hyeon-Soo Park,Sang-Rim Kang,Arulkumar Nagappan,Jin-A Kim,Eun-Hee Kim,Won-Sup Lee,Young-Sool Hah,Hyon-Jong Chung,Su-Jin An,Gon-Sup Kim
Evidence-Based Complementary and Alternative Medicine , 2012, DOI: 10.1155/2012/515901
Abstract: Aim of the Study. Citrus species is used in traditional medicine as medicinal herb in several Asian countries including Korea. Flavonioids became known as various properties, such as anti-oxidants, anti-inflammation and anti-cancer, and so forth. The present study, the anti-cancer effect of flavonioids isolated from Citrus aurantium L. in human gastric cancer AGS cells has been investigated. Materials and Methods. The anti-proliferative activity was assayed using MTT assay. Cell cycle analysis was done using flow cytometry and apoptosis detection was done using by hoechst fluorescent staining and Annexin V-propidium iodide double staining. Western blot was used to detect the expression of protein related with cell cycle and apoptosis. Results. Flavonoids isolated from Citrus aurantium L. have the effect of anti proliferation on AGS cells with IC50 value of 99 μg/mL. Flavonoids inhibited cell cycle progression in the G2/M phase and decrease expression level of cyclin B1, cdc 2, cdc 25c. Flavonoids induced apoptosis through activate caspase and inactivate PARP. Conclusions. Flavonoids isolated from Citrus aurantium L. induced G2/M phase arrest through the modulation of cell cycle related proteins and apoptosis through activation caspase. These finding suggest flavonoids isolated from Citrus aurantium L. were useful agent for the chemoprevention of gastric cancer.
Genome-wide comparative analysis of the Brassica rapa gene space reveals genome shrinkage and differential loss of duplicated genes after whole genome triplication
Jeong-Hwan Mun, Soo-Jin Kwon, Tae-Jin Yang, Young-Joo Seol, Mina Jin, Jin-A Kim, Myung-Ho Lim, Jung Sun Kim, Seunghoon Baek, Beom-Soon Choi, Hee-Ju Yu, Dae-Soo Kim, Namshin Kim, Ki-Byung Lim, Soo-In Lee, Jang-Ho Hahn, Yong Pyo Lim, Ian Bancroft, Beom-Seok Park
Genome Biology , 2009, DOI: 10.1186/gb-2009-10-10-r111
Abstract: We assembled 65.8 megabase-pairs of non-redundant euchromatic sequence of B. rapa and compared this sequence to the Arabidopsis genome to investigate chromosomal relationships, macrosynteny blocks, and microsynteny within blocks. The triplicated B. rapa genome contains only approximately twice the number of genes as in Arabidopsis because of genome shrinkage. Genome comparisons suggest that B. rapa has a distinct organization of ancestral genome blocks as a result of recent whole genome triplication followed by a unique diploidization process. A lack of the most recent whole genome duplication (3R) event in the B. rapa genome, atypical of other Brassica genomes, may account for the emergence of B. rapa from the Brassica progenitor around 8 million years ago.This work demonstrates the potential of using comparative tiling sequencing for genome analysis of crop species. Based on a comparative analysis of the B. rapa sequences and the Arabidopsis genome, it appears that polyploidy and chromosomal diploidization are ongoing processes that collectively stabilize the B. rapa genome and facilitate its evolution.Flowering plants (angiosperms) have evolved in genome size since their sudden appearance in the fossil records of the late Jurassic/early Cretaceous period [1-4]. The genome expansion seen in angiosperms is mainly attributable to occasional polyploidy. Estimation of polyploidy levels in angiosperms indicates that the genomes of most (>90%) extant angiosperms, including many crops and all the plant model species sequenced thus far, have experienced one or more episodes of genome doubling at some point in their evolutionary history [5,6]. The accumulation of transposable elements (TEs) has been another prevalent factor in plant genome expansion. Recent studies on maize, rice, legumes, and cotton have demonstrated that the genome sizes of these crop species have increased significantly due to the accumulation and/or retention of TEs (mainly long terminal repeat retrotr
Two major gate-keepers in the self-renewal of neural stem cells: Erk1/2 and PLCγ1 in FGFR signaling
Jin-A Lee, Deok-Jin Jang, Bong-Kiun Kaang
Molecular Brain , 2009, DOI: 10.1186/1756-6606-2-15
Abstract: Stem cells have two fundamental properties: self-renewal and multipotency. Self-renewal is a cooperative process involving the proliferation and maintenance of an undifferentiated state. The loss of self-renewal properties leads to loss in multipotency, differentiation, and a change in cell fates. Therefore, self-renewal is tightly controlled by the dynamic interplay between intrinsic genetic factors (transcription factors, microRNA, and epigenetic control) and extrinsic factors from the microenvironments (niches) in which stem cells are generated, migrated, and located [1-4]. Among regulatory factors involved in self-renewal, FGF-2 – which primarily acts via the FGF receptor 1 (FGFR1) – is a well-known extrinsic factor maintaining the self-renewal ability of neural stem cells [5,6]. Neural stem cells are found in the adult brain as well as in developing brains, which suggests the essential roles of neurogenesis in adult brain functions [7]. Neurogenesis occurs in response to various environmental signals, such as injury, learning, and memory, or pathological stimuli, which contribute to the remodeling of brain tissues, homeostasis, and regeneration of tissues after an injury [8-10].Many extrinsic factors such as EGF, VEGF, Wnt, Shh, receptor tyrosine kinases, and FGF-2 have been implicated in the self-renewal and differentiation of neural stem cells [11]. Adult neurogenesis depends greatly on FGF-2 signaling [12]. Although the studies on FGF-2 and its role in the maintenance or differentiation of neural stem cells are extensive, the mechanism by which FGFR signaling regulates self-renewal as well as the downstream signaling pathways contributing to the regulation of self-renewal in adult neural stem cells are not well elucidated thus far.Ma et al. [13] have addressed these important issues by characterizing the differential roles of MAPK and PLCγ1 in FGFR1 signaling in the self-renewal of neural stem cells by using a chimeric TrkA-FGFR1.First, to identify the cytop
ESCRT-III subunits Snf7-1 and Snf7-2 differentially regulate transmembrane cargos in hESC-derived human neurons
Jin-A Lee, Lei Liu, Robyn Javier, Anatol C Kreitzer, Celine Delaloy, Fen-Biao Gao
Molecular Brain , 2011, DOI: 10.1186/1756-6606-4-37
Abstract: In this study, we differentiated human embryonic stem cells (hESCs) into postmitotic neurons and characterized the functional properties of these neurons. Moreover, we found that among the three human paralogs of the yeast ESCRT-III subunit Snf7, hSnf7-1 and hSnf7-2 are most abundantly expressed in human neurons. Both hSnf7-1 and hSnf7-2 are required for the survival of human neurons, indicating a non-redundant essential function. Indeed, hSnf7-1 and hSnf7-2 are preferentially associated with CHMP2A and CHMP2B, respectively, and regulate the turnover of distinct transmembrane cargos such as neurotransmitter receptors in human neurons.These findings indicate that different mammalian paralogs of the yeast ESCRT-III subunit Snf7 have non-redundant functions in human neurons, suggesting that ESCRT-III with distinct subunit compositions may preferentially regulate different cargo proteins.Endosomal sorting complex required for transport ESCRTs (ESCRT-0, ESCRT-I, ESCRT-II, and ESCRT-III) are evolutionarily conserved multiunit proteins that are not only required for many fundamental cellular processes, such as multivesicular body (MVB) formation, cytokinesis, virus budding, and autophagy [1-3]. In yeast, ESCRT-III is composed of four subunits, Vps20, Snf7, Vps2, and Vps24, and the Vps20-Snf7 subcomplex is recruited from the cytoplasm to the endosomal membrane and then bound by the Vps2-Vps24 subcomplex [4]. In contrast, there are three human paralogs of Snf7 (hSnf7-1/CHMP4A, hSnf7-2/CHMP4B, and hSnf7-3/CHMP4C) and two paralogs of Vps2 (CHMP2A and CHMP2B) [3]. However, the functional difference between various ESCRT-III paralogs remains unknown.ESCRTs are also implicated in several human diseases including neurodegeneration, cancer, and HIV infection [5]. For instance, a splice site mutation in CHMP2B is associated with frontotemporal dementia linked to chromosome 3 (FTD3) and produces a mutant protein called CHMP2BIntron5, which lacks the C-terminal 36 amino acids [6]. Exp
Gene Expression of Metabolizing Enzymes in Beagle Dog Intestine
Sung-Won Park,Hee Yi,Soo-Min Cho,Kyul Jo,Jin-A Park,Soo-Jean Shin,Hee-Jung Cho,Si-Whan Song,Sang Min Jeong,Ho-Chul Shin
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2011.1947.1955
Abstract: This study was performed to set up a transcriptional database of the intestinal metabolizing enzymes in beagle dogs. The total RNA was isolated from the duodenum and the mRNA expression was measured using GeneChip oligonucleotide arrays. Detected genes from the intestine were about 47% of 43, 035 sequences and total of 79 genes involved metabolizing enzymes. Among the phase I enzymes, dogs exhibited abundant gene expressions of CYP3A12, CYP2B11, LOC610195 (similar to CYP2J2) followed by LOC489851 (similar to CYP3A4), CYP27A1 and CYP51. For phase II enzymes, acetyltransferase ACAT1, glutathione S-transferases GSTA3 and GSTP1, sulfotransferases SULT1A1 and SULT1D, acyltransferases DGAT1 and ACAA1 and glucuronosyltransferase UGCG were highly expressed in duodenum. The dogs expression profiles were compared with those in mice based on gene classification and annotation. Between the two species, the regression of all enzymes (n = 36) with same annotations was 0.496 as andcoefficient of determination (R2) however, two cytochrome P450s including CYP2S1 and CYP4B1 were expressed <5 fold and phase II enzymes including GSTA3, SULT1A1, SULT1D1, TPST1 and UGCG were expressed >5 fold changes in dogs (t-test, p<0.01). In sum, theses data indicated significant differences between beagle dogs and ICR mice in the mRNA expression of both p450s and phase II metabolizing enzymes. These animals are the most widely used species/lines in toxicological and pharmacological screening. Therefore, this database will be useful for predicting and scaling the intestinal drug metabolism between rodents (mice) and non-rodents (dogs).
The Effects of Germanium Biotite Supplement as a Prophylactic Agent against Respiratory Infection in Calves
Myunghwan Jung1, Bock-Gie Jung2, Seung Bin Cha1, Min-Kyoung Shin1, Won-Jung Lee1, Seung Won Shin1, Jin-A Lee2, Yeon-Kwon Jung3, Bong-Joo Lee2 and Han Sang Yoo1*
Pakistan Veterinary Journal , 2012,
Abstract: Germanium biotite, a natural mineral, is comprised of mainly silicate. This mineral showed activities of increase in feed efficiency and non-specific immunostimulation in previous studies. The aims of the present study were to evaluate the prophylactic effects of germanium biotite against respiratory diseases in calves as a feed supplement and investigate the possibilities of the substitution of antibiotics with germanium biotite as feed additive. To achieve these purposes, bovine herpesvirus-1 (BHV-1) and Mannheimia haemolytica serotype A1 were experimentally inoculated into the calves. After challenge, germanium biotite showed a lower cumulative clinical score (CCS) than the control group. In accordance with these clinical results, enhanced clearance of BHV-1, a low infection rate of Mannheimia haemolytica serotype A1, tempered superficial lesions, and moderated histopathological signs were observed in the germanium biotite group, compared with the control group. The results of the present study indicated that germanium biotite had prophylactic effects against bovine respiratory disease and could be a candidate for a new alternative feed supplement in calves, through its effects as a non-specific immune stimulator.
Comparative Study of Imaging Characteristics of I-125 Imaging Using the Siemens Inveon Scanner and Siemens Symbia TruePoint  [PDF]
Young Jun Kim, Ilhan Lim, A Ram Yu, Byung Il Kim, Chang Woon Choi, Sang Moo Lim, Jin Su Kim
Journal of Biomedical Science and Engineering (JBiSE) , 2015, DOI: 10.4236/jbise.2015.810064
Abstract: Objective: Although Iodine-125 (125I) has been widely used for in vitro studies because of its relatively long half-life (60.1 days), 125I imaging is limited because of its low energy (27 - 35 keV), even in an animal-dedicated system. In this study, imaging characteristics of 125I were assessed using a small animal-dedicated imaging system and clinical scanner. Methods: Using the Siemens Inveon and Siemens Symbia TruePoint systems, imaging characteristics such as resolution, sensitivity, and image quality were compared. Mouse high resolution (MHR-0.5), mouse general purpose (MGP-1.0), and mouse high sensitivity (MHS-2.0) collimators were used for the Inveon scanner, and low energy high-resolution (LEHR) and low energy all-purpose (LEAP) collimators were used for the Symbia TruePoint. For animal imaging, 16.8 MBq of 125I was administered to BALB/c mice intravenously, and the planar image and single-photon emission computed tomography (SPECT) were obtained using both scanners. Results: The resolution of 125I for the Inveon scanner was 3.98 mm full width at half maximum (FWHM) at a 30-mm distance with the MHR-0.5 collimator, and the value of Symbia scanner was 8.72 mm FWHM at a 30-mm distance with the LEHR collimator. The sensitivity of 125I for the Inveon scanner was 21.87 cps/MBq, and the value for the clinical scanner was 30.55 cps/MBq. The planar images of mice were successfully obtained at the level of evaluating specific binding in both scanners. Conclusion: 125I small animal imaging can be achieved with a clinical scanner. This result may enhance the utilization of 125I small animal imaging using a clinical scanner.
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