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Search Results: 1 - 10 of 126947 matches for " Jieliang Li "
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Morphine Suppresses IFN Signaling Pathway and Enhances AIDS Virus Infection
Yizhong Wang, Xu Wang, Li Ye, Jieliang Li, Li Song, Nilija Fulambarkar, Wenzhe Ho
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0031167
Abstract: Background Opioids exert a profound influence on immunomodulation and enhance HIV infection and replication. However, the mechanism(s) of their action remains to be determined. We thus investigated the impact of morphine on the intracellular innate antiviral immunity. Methodology/Principal Findings Seven-day-cultured macrophages were infected with equal amounts of cell-free HIV Bal or SIV DeltaB670 for 2 h at 37°C after 24 h of treatment with or without morphine. Effect of morphine on HIV/SIV infection and replication was evaluated by HIV/SIV RT activity assay and indirect immunofluorescence for HIV p24 or SIV p28 antigen. The mRNA expression of cellular factors suppressed or induced by morphine treatment was analyzed by the real-time RT-PCR. We demonstrated that morphine treatment of human blood monocyte-derived macrophages significantly inhibited the expression of interferons (IFN-α, IFN-β and IFN-λ) and IFN-inducible genes (APOBEC3C/3F/3G and 3H). The further experiments showed that morphine suppressed the expression of several key elements (RIG-I and IRF-7) in IFN signaling pathway. In addition, morphine treatment induced the expression of suppressor of cytokine signaling protein-1, 2, 3 (SOCS-1, 2, 3) and protein inhibitors of activated STAT-1, 3, X, Y (PIAS-1, 3, X, Y), the key negative regulators of IFN signaling pathway. Conclusions These findings indicate that morphine impairs intracellular innate antiviral mechanism(s) in macrophages, contributing to cell susceptibility to AIDS virus infection.
(?)-Epigallocatechin gallate inhibits endotoxin-induced expression of inflammatory cytokines in human cerebral microvascular endothelial cells
Jieliang Li, Li Ye, Xu Wang, Jinping Liu, Yizhong Wang, Yu Zhou, Wenzhe Ho
Journal of Neuroinflammation , 2012, DOI: 10.1186/1742-2094-9-161
Abstract: The expression of TNF-α, IL-1β and monocyte chemotactic protein-1 (MCP-1/CCL2) was determined by quantitative real time PCR (qRT-PCR) and ELISA. Intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule (VCAM) in hCMECs were examined by qRT-PCR and Western blotting. Monocytes that adhered to LPS-stimulated endothelial cells were measured by monocyte adhesion assay. Tight junctional factors were detected by qRT-PCR (Claudin 5 and Occludin) and immunofluorescence staining (Claudin 5 and ZO-1). The permeability of the hCMEC monolayer was determined by fluorescence spectrophotometry of transmembrane fluorescin and transendothelial electrical resistance (TEER). NF-kB activation was measured by luciferase assay.EGCG significantly suppressed the LPS-induced expression of IL-1β and TNF-α in hCMECs. EGCG also inhibited the expression of MCP-1/CCL2, VCAM-1 and ICAM-1. Functional analysis showed that EGCG induced the expression of tight junction proteins (Occludin and Claudin-5) in hCMECs. Investigation of the mechanism showed that EGCG had the ability to inhibit LPS-mediated NF-κB activation. In addition, 67-kD laminin receptor was involved in the anti-inflammatory effect of EGCG.Our results demonstrated that LPS induced inflammatory cytokine production in hCMECs, which could be attenuated by EGCG. These data indicate that EGCG has a therapeutic potential for endotoxin-mediated endothelial inflammation.
Lipopolysaccharide Induces Immune Activation and SIV Replication in Rhesus Macaques of Chinese Origin
Rong Bao, Ke Zhuang, Jinbiao Liu, Jianguo Wu, Jieliang Li, Xu Wang, Wen-Zhe Ho
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0098636
Abstract: Background Chronic immune activation is a hallmark of progressive HIV infection and a key determinant of immunodeficiency in HIV-infected individuals. Bacterial lipopolysaccharide (LPS) in the circulation has been implicated as a key factor in HIV infection-related systemic immune activation. We thus investigate the impact of LPS on systemic immune activation in simian immunodeficiency virus (SIV)-infected rhesus macaques of Chinese origin. Methods The animals were inoculated intravenously with SIVmac239. The levels of plasma viral load and host inflammatory cytokines in PBMC were measured by real-time RT-PCR. CD4/CD8 ratio and systemic immune activation markers were examined by flow cytometric analysis of PBMCs. White blood cell and neutrophil counts and C Reactive Protein levels were determined using biochemistry analyzer. The plasma levels of LPS were determined by Tachypleus Amebocyte Lysate (TAL) test. Results The animals inoculated with SIVmac239 became infected as evidenced by the increased plasma levels of SIV RNA and decreased CD4/CD8 ratio. LPS administration of SIV-infected animals induced a transient increase of plasma SIV RNA and immune activation, which was indicated by the elevated expression of the inflammatory cytokines and CD4+HLA-DR+ T cells in PBMCs. Conclusions These data support the concept that LPS is a driving factor in systemic immune activation of HIV disease.
Soybean-derived Bowman-Birk inhibitor inhibits neurotoxicity of LPS-activated macrophages
Jieliang Li, Li Ye, Denise R Cook, Xu Wang, Jinping Liu, Dennis L Kolson, Yuri Persidsky, Wen-Zhe Ho
Journal of Neuroinflammation , 2011, DOI: 10.1186/1742-2094-8-15
Abstract: Mixed cortical neural cultures from rat were used as target cells for testing neurotoxicity induced by LPS-treated macrophage supernatant. Neuronal survival was measured using a cell-based ELISA method for expression of the neuronal marker MAP-2. Intracellular reactive oxygen species (ROS) production in macrophages was measured via 2', 7'-dichlorofluorescin diacetate (DCFH2DA) oxidation. Cytokine expression was determined by quantitative real-time PCR.LPS treatment of macrophages induced expression of proinflammatory cytokines (IL-1β, IL-6 and TNF-α) and of ROS. In contrast, BBI pretreatment (1-100 μg/ml) of macrophages significantly inhibited LPS-mediated induction of these cytokines and ROS. Further, supernatant from BBI-pretreated and LPS-activated macrophage cultures was found to be less cytotoxic to neurons than that from non-BBI-pretreated and LPS-activated macrophage cultures. BBI, when directly added to the neuronal cultures (1-100 μg/ml), had no protective effect on neurons with or without LPS-activated macrophage supernatant treatment. In addition, BBI (100 μg/ml) had no effect on N-methyl-D-aspartic acid (NMDA)-mediated neurotoxicity.These findings demonstrate that BBI, through its anti-inflammatory properties, protects neurons from neurotoxicity mediated by activated macrophages.Inflammation plays a critical role in neurodegenerative diseases such as Parkinson's disease, multiple sclerosis, Alzheimer's disease, and HIV-associated dementia (HAD). Activation of microglia, the intrinsic macrophages in the central nervous system (CNS) [1], is a characteristic feature of most neurodegenerative diseases upon systemic infection. Mounting evidence indicates that macrophage/microglia activation contributes to inflammation and neuronal injury in a number of neurological disorders [2,3]. However, the cellular and molecular relationships between infections outside the CNS and potential neuronal loss within the CNS is elusive. It is known that in response to certain
Evaluation of eco-environmental quality based on artificial neural network and remote sensing techniques
基于人工神经网络的生态环境质量遥感评价

LI Hongyi,SHI Zhou,SHA Jinming,CHENG Jieliang,
李洪义
,史舟,沙晋明,程街亮

应用生态学报 , 2006,
Abstract: In the present study, vegetation, soil brightness, and moisture indices were extracted from Landsat ETM remote sensing image, heat indices were extracted from MODIS land surface temperature product, and climate index and other auxiliary geographical information were selected as the input of neural network. The remote sensing eco-environmental background value of standard interest region evaluated in situ was selected as the output of neural network, and the back propagation (BP) neural network prediction model containing three layers was designed. The network was trained, and the remote sensing eco-environmental background value of Fuzhou in China was predicted by using software MATLAB. The class mapping of remote sensing eco-environmental background values based on evaluation standard showed that the total classification accuracy was 87.8%. The method with a scheme of prediction first and classification then could provide acceptable results in accord with the regional eco-environment types.
Identification of tumor metastasisrelated gene TMSG-1 by mRNA differential display
Chunshu Ma,Yuxin Liu,Jie Zheng,Weigang Fang,Jiangfeng You,Jieliang Wang,Xianglin Cui,Bingquan Wu
Science China Life Sciences , 2002, DOI: 10.1360/02yc9061
Abstract: To investigate genes involved in cancer metastasis, mRNA differential display was used to compare the levels of gene expression of two cancer sublines derived from prostate carcinoma cell PC-3M that had different metastatic potentials. The differentially expressed genes were confirmed by Northern blot, and sequenced. The fulllength cDNA of a tumor metastasis suppressor gene (TMSG-1) was obtained by using EST assembling and verified by RT-PCR and sequencing. The results showed that expression levels of TMSG-1 were lower in the highly metastatic cell line 1E8, compared with the nonmetastatic cell line 2B4. The difference was significant. Fulllength cDNA of TMSG-1 was about 2 kb, containing an open reading frame that encoded a protein of 230 amino acids. GenBank Blastn showed no marked homology with known genes. The functional prediction of amino acids sequence encoded by TMSG-1 gene indicated TMSG-1 protein was transmembrane protein, with 3 transmembrane domains, 3 putative protein kinase phosphorylation sites, 2 casein kinase II phosphorylation sites and 1 Nmyristoylation site. The pattern of TMSG1 expression in 6 types of human tumor tissues indicated levels of transcripts were the highest in prostate carcinoma. TMSG-1 had lower expression in metastases of lung carcinoma compared to primary lung carcinoma. Similarly the expression levels were higher in welldifferentiated colon carcinoma than that in poorly differentiated colon carcinoma. TMSG-1 could also be detected in breast, ovarian, and pancreatic carcinoma. In 9 samples of primary gastric carcinoma tissues, RT-PCR and densitometric analysis demonstrated TMSG-1 expression levels in samples with lymph node metastases had a decreased tendency, compared to those without lymph node metastases. The difference was significant by student’s t test (P< 0.05). These results indicated TMSG-1 expression levels were inversely correlated with tumor metastatic potential.
Primary functional identification of gene TMSG-1
Chunshu Ma,Junyu Ning,Jiangfeng You,Lin Liu,Jieliang Wang,Xianglin Cui,Bingquan Wu,Jie Zheng
Science China Life Sciences , 2003, DOI: 10.1360/02yc0159
Abstract: TMSG-1 was a tumor metastasis-related gene identified using mRNA differential display, whose expression level was lower in cancer cell lines with higher metastatic potential and in tumor tissue with metastasis. TMSG-1 was transfected to prostate cancer cell line (PC-3M-1E8) with high metastatic potential to observe the effects of increased expression of TMSG-1 on V-ATPase activity, intracellular pH and cell apoptosis. Subcellular localization of the encoded protein of TMSG-1 was determined by using GFP. Results showed that there were no differences of V-ATPase activity among parental PC-3M-1E8 cell line, pcDNA3 transfectant and anti-TMSG-1 transfectant, whereas the V-ATPase activity was significantly higher in TMSG-1 transfectant than that in parental PC-3M-1E8 cell line, pcDNA3 transfectant and Anti-TMSG-1 transfectant (p<0.001). Intracellular pH (pHi) was detected by using the pH-dependent fluorescence probe BECEF. Results showed the pHi was significantly increased in TMSG-1 transfectant. Cell apoptosis assay demonstrated cell apoptosis was significantly higher in -1 transfectant (p<0.01) and BCL2 expression was down regulated. Subcellular localization of TMSG-1 protein showed TMSG-1 was a transmembrane protein, which predicted TMSG-1 protein was located in cytoplasm system, such as endoplasmic reticulum and mitochondrial. These results indicated TMSG-1 up regulation in prostate cancer cell line could promote V-ATPase activity, increase pHi and cell apoptosis, and inhibit the expression of BCL2.
Integrated Analysis of DNA Methylation and RNA Transcriptome during In Vitro Differentiation of Human Pluripotent Stem Cells into Retinal Pigment Epithelial Cells
Zhenshan Liu, Rongfeng Jiang, Songtao Yuan, Na Wang, Yun Feng, Ganlu Hu, Xianmin Zhu, Kevin Huang, Jieliang Ma, Guotong Xu, Qinghuai Liu, Zhigang Xue, Guoping Fan
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0091416
Abstract: Using the paradigm of in vitro differentiation of hESCs/iPSCs into retinal pigment epithelial (RPE) cells, we have recently profiled mRNA and miRNA transcriptomes to define a set of RPE mRNA and miRNA signature genes implicated in directed RPE differentiation. In this study, in order to understand the role of DNA methylation in RPE differentiation, we profiled genome-scale DNA methylation patterns using the method of reduced representation bisulfite sequencing (RRBS). We found dynamic waves of de novo methylation and demethylation in four stages of RPE differentiation. Integrated analysis of DNA methylation and RPE transcriptomes revealed a reverse-correlation between levels of DNA methylation and expression of a subset of miRNA and mRNA genes that are important for RPE differentiation and function. Gene Ontology (GO) analysis suggested that genes undergoing dynamic methylation changes were related to RPE differentiation and maturation. We further compared methylation patterns among human ESC- and iPSC-derived RPE as well as primary fetal RPE (fRPE) cells, and discovered that specific DNA methylation pattern is useful to classify each of the three types of RPE cells. Our results demonstrate that DNA methylation may serve as biomarkers to characterize the cell differentiation process during the conversion of human pluripotent stem cells into functional RPE cells.
Primary functional identification of gene TMSG-1
MA Chunshu,NING Junyu,YOU Jiangfeng,LIU Lin,WANG Jieliang,CUI Xianglin,WU Bingquan,ZHENG Jie,
马春树
,宁钧宇,由江峰,柳林,王洁良,崔湘琳,吴秉铨,郑杰

中国科学C辑(英文版) , 2003,
Abstract: TMSG-1 was a tumor metastasis-related gene identified using mRNA differential dis-play, whose expression level was lower in cancer cell lines with higher metastatic potential and in tumor tissue with metastasis. TMSG-1 was transfected to prostate cancer cell line (PC-3M-1E8) with high metastatic potential to observe the effects of increased expression of TMSG-1 on V-ATPase activity, intracellular pH and cell apoptosis. Subcellular localization of the encoded pro-tein of TMSG-1 was determined by using GFP. Results showed that there were no differences of V-ATPase activity among parental PC-3M-1E8 cell line, pcDNA3 transfectant and anti-TMSG-1 transfectant, whereas the V-ATPase activity was significantly higher in TMSG-1 transfectant than that in parental PC-3M-1E8 cell line, pcDNA3 transfectant and Anti-TMSG-1 transfectant (p<0.001). Intracellular pH (pHi) was detected by using the pH-dependent fluorescence probe BECEF. Re-sults showed the pHi was significantly increased in TMSG-1 transfectant. Cell apoptosis assay demonstrated cell apoptosis was significantly higher in -1 transfectant (p<0.01) and BCL2 expres-sion was down regulated. Subcellular localization of TMSG-1 protein showed TMSG-1 was a transmembrane protein, which predicted TMSG-1 protein was located in cytoplasm system, such as endoplasmic reticulum and mitochondrial. These results indicated TMSG-1 up regulation in prostate cancer cell line could promote V-ATPase activity, increase pHi and cell apoptosis, and inhibit the expression of BCL2.
Beneficial Experience from Teaching and Education to Research and Development  [PDF]
Li Li
Creative Education (CE) , 2012, DOI: 10.4236/ce.2012.37B039
Abstract:

Teaching and Education (T&E) constitute the most important activity in knowledge transfer from generation to generation. This can explain why government organizations consider the training of highly qualified personnel as one of the most important criteria in the selection of research and development (R&D) grant applications. A university professor should thus not only play the role of researcher, but also that of teacher. T&E and R&D combine to form an inseparable relationship for university professors. By shooting for excellence in T&E, we could get a new perception of a familiar field or initiate a brand new field altogether, which would in turn enhance our research. The quest for excellence in R&D leads to deeper and better understanding of materials taught, and progress in R&D enriches our T&E endeavors. Here, the author shares a beneficial experience from T&E to R&D.

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