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Search Results: 1 - 10 of 18412 matches for " Jianhao Peng "
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Fluorescence Resonance Energy Transfer Assay for High-Throughput Screening of ADAMTS1 Inhibitors
Jianhao Peng,Lili Gong,Kun Si,Xiaoyu Bai,Guanhua Du
Molecules , 2011, DOI: 10.3390/molecules161210709
Abstract: A disintegrin and metalloprotease with thrombospondin type I motifs-1 (ADAMTS1) plays a crucial role in inflammatory joint diseases and its inhibitors are potential candidates for anti-arthritis drugs. For the purposes of drug discovery, we reported the development and validation of fluorescence resonance energy transfer (FRET) assay for high-throughput screening (HTS) of the ADAMTS1 inhibitors. A FRET substrate was designed for a quantitative assay of ADAMTS1 activity and enzyme kinetics studies. The assay was developed into a 50-μL, 384-well assay format for high throughput screening of ADAMTS1 inhibitors with an overall Z’ factor of 0.89. ADAMTS1 inhibitors were screened against a diverse library of 40,960 total compounds with the established HTS system. Four structurally related hits, naturally occurring compounds, kuwanon P, kuwanon X, albafuran C and mulberrofuran J, extracted from the Chinese herb Morus alba L., were identified for further investigation. The results suggest that this FRET assay is an excellent tool, not only for measurement of ADAMTS1 activity but also for discovery of novel ADAMTS1 inhibitors with HTS.
The Vasorelaxant Mechanisms of a Rho Kinase Inhibitor DL0805 in Rat Thoracic Aorta
Lili Gong,Jianhao Peng,Lianhua Fang,Ping Xie,Kun Si,Xiaozhen Jiao,Liping Wang,Guanhua Du
Molecules , 2012, DOI: 10.3390/molecules17055935
Abstract: Rho-kinase has been suggested as a potential therapeutic target in the treatment of cardiovascular diseases. The Rho-kinase signaling pathway is substantially involved in vascular contraction. The aim of the present study was to evaluate the vasorelaxant effects of Rho kinase inhibitor DL0805 in isolated rat aortic rings and to investigate its possible mechanism(s). It was found that DL0805 exerted vasorelaxation in a dose-dependent manner in NE or KCl-induced sustained contraction and partial loss of the vasorelaxation under endothelium-denuded rings. The DL0805-induced vasorelaxation was significantly reduced by the nitric oxide synthase inhibitor Nω-nitro-L-arginine methyl ester, the guanylate cyclase inhibitor methylene blue and the cyclooxygenase inhibitor indomethacin. The voltage-dependent K+ channel blocker 4-aminopyridine remarkably attenuated DL0805-induced relaxations. However, the ATP-sensitive K+ channel blocker glibenclamide and Ca2+-activated K+ channel blocker tetraethylammonium did not affect the DL0805-induced relaxation. In the endothelium-denuded rings, DL0805 also reduced NE-induced transient contraction and inhibited contraction induced by increasing external calcium. These findings suggested that DL0805 is a novel vasorelaxant compound associated with inhibition of Rho/ROCK signaling pathway. The NO-cGMP pathway may be involved in the relaxation of DL0805 in endothelium-intact aorta. The vasorelaxant effect of DL0805 is partially mediated by the opening of the voltage-dependent K+ channels.
Experimental Research of Optimal Die-forging Technological Schemes Based on Orthogonal Plan
Jianhao Tan,Jing Zhang,Fu Guo
Modern Applied Science , 2009, DOI: 10.5539/mas.v3n3p26
Abstract: Some problems on setting up die-forging technological scheme design criteria by means of traditional methods are analyzed. The idea of mining die-forging technological schemes based on orthogonal plan is pointed out. The height and width of the hub are selected as key factors from many ones which influence the die-forging technological schemes of axisymmetric forging, then, in connection with the two factors, the relative experiments are arranged by using the two-factor-twice-composition orthogonal plan. Flash metal consumption is choosed from a lot of factors as object function whose values are measured in the experiments. When the height and width of the hub are the constant, from several experimental schemes, the technological scheme which makes flash metal consumption be the least is selected as the optimal scheme, so the design criteria of optimal die-forging technological schemes are got. Because of adopting orthogonal plan in arranging the experiments, the design and manufacturing period of forging is reduced, the developing cost of forging is cut down, and the raw and processed material consumption is decreased. In laying down the design criteria of optimal die-forging technological schemes, optimal technology is combined with artificial intelligence. In determining the relations among so many factors in die-forging technological schemes, expert’s experiences are used, and experimental results are dealt with by means of association rule mining technology, which makes the decision of die-forging schemes more reasonable and practical.
Machine Learning Emulation in Nature-inspired Computation Systems
Jianhao Tan,Jing Zhang,Fu Guo
Computer and Information Science , 2009, DOI: 10.5539/cis.v2n3p15
Abstract: The whole frame of nature_inspired computation systems is inquired into, the characteristics of machine learning in nature_inspired computation systems are researched, and a particular scheme on machine learning in nature_inspired computation systems is designed with environment being gathered present data; study unit adopting fuzzy optimizatio algorithm based on genetic algorithm; knowledge base adopting fuzzy optimization BP neural networks; executive unit being complicated industry process. The fuzzy optimizatio learning algorithm of fuzzy optimization BP neural networks is built, the flow chart of the algorithm is constructed, and the emulation test is made. At last, the design criteria of flash metal comsuption are obtained, and the stability of the algorithm is verified through this example. The result shows that machine learning makes nature_inspired computation systems be able to gain know; edge automatically, their quality improved, their intelligent level advanced, and machine learning will greatly influence the memory mode, information input mode and system structures of nature_inspired computation systems.
An Improved Clustering Algorithm Based on Density Distribution Function
Jianhao Tan,Jing Zhang,Weixiong Li
Computer and Information Science , 2010, DOI: 10.5539/cis.v3n3p23
Abstract: Some characteristics and week points of traditional density-based clustering algorithms are deeply analysed , then an improved way based on density distribution function is put forward. K Nearest Neighbor( KNN ) is used to measure the density of each point, then a local maximum density point is defined as the center point.. By means of local scale, classification is extended from the center point. For each point there is a procedure to find whether it is a core point by a radius scale factor. Then the classification is extended once again from the core point until the density descends to the given ratio of the density of the center point. The tests show that the improved algorithm greatly improves the sensitivity of density-based clustering algorithms to parameters and enhances the clustering effect of the high-dimensional data sets with uneven density distribution.
A Kind of CTA Bone Removal Technology Based on the Improved Watershed Algorithm
Tan Jianhao,Zhang Jing,Wang Ya
Computer and Information Science , 2012, DOI: 10.5539/cis.v5n5p81
Abstract: Based on analyzing the drawbacks existing in traditional watershed algorithms for bone removal from CTA (Computed Tomography Angiography, CTA), this paper presents an improved interactive watershed algorithm. The improved watershed algorithm is based on sorting and graded overflow of fast watershed algorithms, and the merging process of catchment basins is intervened by a merging threshold which is given by users so as to take an effect on segmentation results. The algorithm can record such basic information as the labels and ridge points of each basin in graded overflow basin marking, set merging threshold by means of user interaction for controlling merging process effectively, and suppresses over segmentation. At last, the improved algorithm is applied to bone removal from CTA Images, and three-dimensional rendering is taken for CTA Images after bone removal. The experimental results indicate that the improved algorithm prevents over segmentation in watershed transformation effectively and removes bone structure accurately.
Factors Affecting the Duration between Symptom Onset and Diagnosis in Patients with Gastric Cancer  [PDF]
Jianhao Yin, Yong Zhang, Jianguo Lai, Yao Tang, Lei Meng, Gang Xu, Haonan Wang, Chengxue Dang
Journal of Biosciences and Medicines (JBM) , 2016, DOI: 10.4236/jbm.2016.49005
Abstract: Background: The prognosis of patients with tumors is partly influenced by the stage when patients are diagnosed. Therefore, the time of confirmed diagnosis after onset can be considered as a prognostic predictor. Although a few of studies work on it, factors that influence the duration remain poorly understood in different areas. Methods: This retrospective study involved patients with gastric cancer. Participants completed questionnaires by either phone or face-to-face interviews. Results: Females are with a longer interval than males. Farms lived in rural area and with New Rural Cooperative Medical Scheme are associated with a longer time interval. Patients whose income is mainly from their spouses also have a longer interval. Abdominal pain seems to be associated with longer interval time, while vomiting leads to a shorter time interval. Taking medicine after symptoms appear is associated with longer time intervals, especially those take medicine nonstandardly and more than 2 weeks. Conclusions: The results demonstrate that the medical resource in rural area should still be enhanced; meanwhile taking medicine without doctor’s advice should be cut down. Additional efforts must be made to devise strategies to properly guide patients’ behavior.
Risk Factors for Radiographic Tibiofemoral Knee Osteoarthritis: The Wuchuan Osteoarthritis Study
Jianhao Lin,Rujun Li,Xiaozheng Kang,Hu Li
International Journal of Rheumatology , 2010, DOI: 10.1155/2010/385826
Abstract: Objective. To investigate the risk factors of radiographic tibiafemoral knee osteoarthritis (OA). Methods. A population-based cross-sectional survey was conducted in Wuchuan County. A questionnaire and bilateral weight-bearing posterior-anterior semi-flexed knee radiographs were completed and read for Kellgren and Lawrence (K/L) grade and joint space narrowing (JSN; 0–3 scale) in each compartment. An logistic regression analysis was performed for radiographic tibiafemoral, lateral compartment, and medial compartment knee OA, respectively. Adjusted odds ratios (OR) and 95% confidence intervals (CI) were calculated. Results. Age, sex, and knee injury were strongly associated with tibiafemoral, lateral and medial compartment knee OA. BMI also had a dose-response relationship with them. Physical activity level, and physical activity exposure at work, not significantly though, were associated with an elevated risk for this three kinds of knee OA. Conclusions. Physical activity exposure increased the risk of knee OA. It was likely to be the heavier physical activity in Wuchuan osteoarthritis study that counteracted the BMI gap compared with the Beijing and the Framingham OA study. We verified that Chinese had a more valgus alignment of the knee compared with Caucasian population, and this provide a possible explanation why Chinese have a higher prevalence of lateral compartment OA. 1. Introduction Knee osteoarthritis (OA) is an important cause of pain and disability in old population, and it is becoming increasingly prevalent worldwide due to its association with an aging population and due to a growing prevalence of obesity [1]. To investigate the prevalence and risk factors of knee osteoarthritis, many population-based observational studies have been conducted worldwide. The etiology of knee OA is believed to be multifactorial, and the following risk factors have been identified: heredity [2–4], obesity [5–8], injury [8–11], and physical workload [12–15]. However, most of the large-scale studies were conducted in North America and Europe, with scant information from less developed regions Reference [16], and most of the study participants were based on urban residents or workers, while people in rural areas, especially farmers, were rarely investigated. In fact, farmers form a large proportion of the population in less developed regions, most of whom have to endure heavy physical workload until an old age. The prevalence and risk factors of knee OA among these people might be different. We conducted a population-based cross-sectional survey in Wuchuan
Echinoderms Have Bilateral Tendencies
Chengcheng Ji, Liang Wu, Wenchan Zhao, Sishuo Wang, Jianhao Lv
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0028978
Abstract: Echinoderms take many forms of symmetry. Pentameral symmetry is the major form and the other forms are derived from it. However, the ancestors of echinoderms, which originated from Cambrian period, were believed to be bilaterians. Echinoderm larvae are bilateral during their early development. During embryonic development of starfish and sea urchins, the position and the developmental sequence of each arm are fixed, implying an auxological anterior/posterior axis. Starfish also possess the Hox gene cluster, which controls symmetrical development. Overall, echinoderms are thought to have a bilateral developmental mechanism and process. In this article, we focused on adult starfish behaviors to corroborate its bilateral tendency. We weighed their central disk and each arm to measure the position of the center of gravity. We then studied their turning-over behavior, crawling behavior and fleeing behavior statistically to obtain the center of frequency of each behavior. By joining the center of gravity and each center of frequency, we obtained three behavioral symmetric planes. These behavioral bilateral tendencies might be related to the A/P axis during the embryonic development of the starfish. It is very likely that the adult starfish is, to some extent, bilaterian because it displays some bilateral propensity and has a definite behavioral symmetric plane. The remainder of bilateral symmetry may have benefited echinoderms during their evolution from the Cambrian period to the present.
UDP-glucose pyrophosphorylase2 (OsUgp2), a pollen-preferential gene in rice, plays a critical role in starch accumulation during pollen maturation
Hong Mu,JianHao Ke,Wei Liu,ChuXiong Zhuang,WingKin Yip
Chinese Science Bulletin , 2009, DOI: 10.1007/s11434-008-0568-y
Abstract: UDP-glucose pyrophosphorylase (UGPase) is predominantly present and plays significant role in carbohydrate metabolism in plants. Two homologous UGPase genes, OsUgp1 and OsUgp2, exist in rice genome. OsUgp1 has recently been reported to be essential for callose deposition during pollen mother cell and meiosis stages as well as for seed carbohydrate metabolism. In this study, a full-length cDNA of OsUgp2 was isolated from rice anther. Northern blot and RNA in situ hybridization indicated that the expression of OsUgp2 was preferentially in pollen and developmentally regulated. No transcripts were found in leaf, stem, lemma/palea, ripening grain and florets before the uninucleate microspore developmental stage, but a large quantity of OsUgp2 mRNA was found in pollen at the binucleate and mature stages. The immunolocalization of OsUgp2 showed a similar expression pattern to that by RNA in situ hybridization. The function of OsUgp2 was investigated by dsRNA-mediated transcriptional gene silencing. The pollen fertility of 16 independent transgenic rice plants was found between 25% and 90%, which was correlated with the amount of OsUgp2 mRNA. The results of morphological changes and starch variation during pollen development in transgenic rice showed that the abnormal feature of pollen development appeared after the uninucleate microspore stage. Starch failed to accumulate in pollen and thus led to sterile pollens. These results demonstrated that OsUgp2 is a pollen-preferential “late gene” and plays a key role during pollen maturation, especially for starch accumulation. OsUgp2 complements OsUgp1 to fulfill the UGPase’s functions necessary for the full process of pollen development.
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