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Search Results: 1 - 10 of 34612 matches for " Jiangang Zhou "
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Cloning of a novel gene encoding human thioredoxin-like protein
Yan Zhou,Meihui Pan,Jiangang Yuan,Guangwei Du,Boqin Qiang
Chinese Science Bulletin , 1999, DOI: 10.1007/BF03183487
Abstract: mRNA differential display (DDRT-PCR) has been used to analyze different human fetal brain tissues of different developmental stages (13- and 33-week). According to the sequence of one EST obtained in this assay, a pair of primers have been designed to screen the arrayed human fetal brain cDNA library. A 1.2-kb cDNA clone has been found. This cDNA consists of an 867 bp open reading frame, a 132 bp 5′ untranslated sequence and a 209 bp 3′ untranslated sequence with a typical polyadenylation signal. The coding region predicts a protein of 289 amino acids. Its N-terminal of 105 residues is highly homologous to human thioredoxin, while no homology has been found in the databases with its C-terminal of 184 residues. Its N-terminal region also contains the conserved active site sequence CGPC (Cys-Gly-Pro-Cys) of thioredoxin. It was named human Thioredoxin-like gene (hTRXL).
A Study on Credit Appraisal System of C2C E-Commerce
—Based on the Analysis of Taobao

Jiangang Pang, Zhiying Liu
American Journal of Industrial and Business Management (AJIBM) , 2014, DOI: 10.4236/ajibm.2014.412078
Abstract: In the virtual network environment, the C2C e-commerce has some problems in product quality, service, credit speculation. At present the credit appraisal system offered by domestic C2C e-commerce website ensures the network security to a certain extent while it has some problems in identity verification, evaluation rules and model, credit of buyers and sellers and so on. Focused on the largest national C2C shopping website—Taobao, this article mainly analyzes the present situations and problems of the credit appraisal system, puts forward some improved suggestions of the real-name authentication, credit and transaction appraisal rules and so forth, and then establishes improved credit appraisal system.
Cloning and expression of a novel humanHCUTA cDNA
Wenqin Luo,Jianhe Chen,Haijun Zhou,Xiaowei Huang,Yan Zhou,Jiangang Yuan,Boqin Qiang
Chinese Science Bulletin , 2000, DOI: 10.1007/BF03182907
Abstract: Copper is one of the most important trace elements to life. HumanHCUTA is a novel cDNA encoding a 156aa protein, which may participate in human copper tolerance system. The HCUTA protein is highly similar to protein CUT A1 ofE. coli. The whole opening reading frame ofHCUTA cDNA was amplified by PCR and cloned into pET28a + express vector, and the HCUTA protein was effectively expressed inE. coli BL21 (DE3).
Negative Feedback Regulation of Wnt4 Signaling by EAF1 and EAF2/U19
Xiaoyang Wan,Wei Ji,Xue Mei,Jiangang Zhou,Jing-xia Liu,Chengchi Fang,Wuhan Xiao
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0009118
Abstract: Previous studies indicated that EAF (ELL-associated factor) family members, EAF1 and EAF2/U19, play a role in cancer and embryogenesis. For example, EAF2/U19 may serve as a tumor suppressor in prostate cancer. At the same time, EAF2/U19 is a downstream factor in the non-canonical Wnt 4 signaling pathway required for eye development in Xenopus laevis, and along with EAF1, contributes to convergence and extension movements in zebrafish embryos through Wnt maintenance. Here, we used zebrafish embryos and mammalian cells to show that both EAF1 and EAF2/U19 were up-regulated by Wnt4 (Wnt4a). Furthermore, we found that EAF1 and EAF2/U19 suppressed Wnt4 expression by directly binding to the Wnt4 promoter as seen in chromatin immunoprecipitation assays. These findings indicate that an auto-regulatory negative feedback loop occurs between Wnt4 and the EAF family, which is conserved between zebrafish and mammalian. The rescue experiments in zebrafish embryos showed that early embryonic development required the maintenance of the appropriate levels of Wnt4a through the feedback loop. Others have demonstrated that the tumor suppressors p63, p73 and WT1 positively regulate Wnt4 expression while p21 has the opposite effect, suggesting that maintenance of appropriate Wnt4 expression may also be critical for adult tissue homeostasis and prevention against tumor initiation. Thus, the auto-regulatory negative feedback loop that controls expression of Wnt4 and EAF proteins may play an important role in both embryonic development and tumor suppression. Our findings provide the first convincing line of evidence that EAF and Wnt4 form an auto-regulatory negative feedback loop in vivo.
Cloning of human and mouseGRY-RBP cDNA
Guangwei Du,Meihui Pan,Yan Zhou,Jianhe Chen,Hui Yao,Jiangang Yuan,Boqin Qiang
Chinese Science Bulletin , 2000, DOI: 10.1007/BF02909766
Abstract: After screening human fetal brain cDNA library, a novel human cDNA encoding an RNA-binding protein was cloned and namedGRY-RBP. HumanGRY-RBP cDNA is 2 932 bp in length, and encodes a protein of 623 amino acids with a deduced molecular mass of 69.6 ku. Human GRY-RBP contains three RNA recognition motifs (RRMs) and a glycine (G), tyrosin (Y)-rich C-terminus. Computer searches with sequence database revealed its moderate homology with RRMs of many RRM RNA-binding proteins. In this study, the full coding region and partial non-coding region of mouseGRY-RBP cDNA were amplified by PCR using mouse brain cDNA library as template. Three transcripts ofGry-rbp are expressed in all tissues as shown by Northern blot, but the amounts in heart, planceta and skeletal muscle are considerably higher than those in other tissues.
Isolation and expression pattern analysis of novel ESTs from human fetal brain
Meihui Pan,Jiangang Yuan,Guangwei Du,Yan Zhou,Hui Yao,Boqin Qiang
Chinese Science Bulletin , 1998, DOI: 10.1007/BF02883379
Abstract: In order to learn the mechanism of brain development and differentiation, 90 expressed sequence tags (ESTs) were isolated by differential display from the cerebrum and cerebellum of 13-week and 33-week fetal brains. After searching database, 74 of them represented novel genes, some of them were homologous to the brain development related genes. Using total cDNA probes, 79 of the ESTs and their expression differences in fetal brain were further characterized.
Cloning and expression analysis ofMBLL cDNA
Guangwei Du,Yan Zhou,Jianhe Chen,Junhua Wang,Bin Yin,Jiangang Yuan,Boqin Qiang
Chinese Science Bulletin , 2000, DOI: 10.1007/BF02886038
Abstract: Thembl (muscleblind) gene ofDrosophila encodes a nuclear protein which contains two Cys3His motifs. The mutation ofmbl gene will disturb the differentiation of all theDrosophila’s photoreceptors. Primers have been designed according to human EST086139, which is highly homologous tombl gene. Human fetal brain cDNA library has been screened and a novel cDNA clone has been obtained. The 2595 bp cDNA, designatedMBLL (muscleblind-like), contains an open reading frame which encodes 255 amino acids and has 4 Cys3His motifs (GenBank Acc. AF061261). The amino acids sequence shares high homology toDrosophila’s mbl. The Northern blot and RNA dot blot hybridization of 43 human adult tissues and 7 fetal tissues show thatMBLL is a widely expressed gene, but the expression amounts differ in these tissues.
Isolation and cloning of a novel cDNALDB1 encoding human LIM domain binding protein
Yan Zhou,Guangwei Du,Junhua Wang,Jianhe Chen,Jiangang Yuan,Boqin Qiang
Chinese Science Bulletin , 1999, DOI: 10.1007/BF02886138
Abstract: Primers for screening cDNA library have been designed according to EST AA453734 which is corresponding to the mouse LIM domain binding protein Ldbl. Arrayed human fetal brain cDNA library has been screened by PCR and routine hybridization method. A 2398 bp-cD-NA clone has been obtained. The cDNA encodes a 347 amino acids protein highly homologous to the mouse Ldbl,Xenopus Xldbl andDrosophila Chip. It also contains an LIM binding domain and a nuclear localization signal. It has been namedLDB1 ( LIM domain binding protein 1), GenBank accession number is AF052389. Northern blot showed a 2.4 kb band, and the expression amounts ofLDBI in heart, brain and lung were considerably higher than those in other tissues.
The minimization of non-equilibrium plasma source at high pressure
MinDi Bai,XiuMei Qiu,Dong Liu,Bo Yang,JianGang Zhou,XiaoHong Xue,JianLong Gu
Chinese Science Bulletin , 2008, DOI: 10.1007/s11434-008-0445-8
Abstract: The density of plasma produced by atmospheric non-equilibrium plasma source is the function of energy dissipation rate in ionization electric field and gas particles momentum. The experiment shows that the plasma density highly rises with the increasing of energy dissipation rate and gas particles momentum. When the energy dissipation rate of activation field is 2.18 Wh/m3 and the average gas particles momentum is 109×10 22 g·m/s, the air throughput of plasma source whose volume is only 2.5 cm3 can be up to 12 m3/h and the density of plasma can be up to 1010 cm 3. The research can develop a method of producing minitype plasma source which is low energy consumption but high ion concentration used for chemical industry, environmental engineering and military.
Cloning and identification ofPHF2 cDNA and its alternatively spliced transcripts
Junhua Wang,Guangwei Du,Yan Zhou,Bin Yin,Jiangang Yuan,Boqin Qiang
Chinese Science Bulletin , 1999, DOI: 10.1007/BF02885987
Abstract: Genes encoding proteins with PHD (plant homeodomain) finger motif (C4HC3) are highly conserved fromArabidopis toHomo sapiens. One of the major functions of these genes is regulating the expression of homeotic genes during the stage of embryonic development. They play a role in cell-cycle and cell differentiation and seem to be related with some human malignant diseases, such as leukemia. A human placenta cDNA library has been screened with cDNA probe amplified by PCR. The PCR primers have been designed according to theM96 (a mouse gene encoding a protein with PHD domain) homologous data in dbEST. A 2.1 kb insert fragment in a positive cDNA clone has been isolated and sequenced. This new full-length cDNA is namedPHF2 (GenBank Acc: AF052205). The putative protein composed of 567 aa has two typical PHD fingers at its N-terminus. Meanwhile it is identified that there are several alternatively spliced transcripts ofPHF2 in different human tissues through the PCR amplification, Northern blot hybridization and analysis of genomic DNA structure.
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