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Search Results: 1 - 10 of 209659 matches for " Jhon Fredy; López-López "
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Detección, identificación y localización geográfica de Begomovirus que afectan al tomate en Colombia
Vaca-Vaca,Juan Carlos; Betancurt-Pérez,Jhon Fredy; López-López,Karina;
Revista Colombiana de Biotecnología , 2011,
Abstract: the begomoviruses (family geminiviridae) have recently emerged as a serious pathogens of agronomic and horticultural crops in subtropical and tropical regions of world. these viruses are transmitted by the whitefly (bemisia tabaci) that recently has got a significant increase in its populations and also has been associated with the appearance of geminivirus typical symptoms in tomato crops around colombia. tomato samples with typical begomovirus symptoms were collected in five different departments, comprising the mayor tomato growing areas of the country. begomovirus were detected by polymerase chain reaction (pcr) or dot blot hybridization in all tomato samples collected in whole tomato growing areas of the country. with exception for valle del cauca, this is the first report of tomato-infecting begomovirus in antioquia, santander, boyacá and cundinamarca departments. also this is the first report of tomato-infecting begomovirus crops located above 1500 masl in colombia.
Distribución y diversidad genética de Begomovirus que infectan tomate (Solanum lycopersicum L) en Colombia
Vaca-Vaca,Juan Carlos; Betancur-Pérez,Jhon Fredy; López-López,Karina;
Revista Colombiana de Biotecnología , 2012,
Abstract: diseased caused by begomoviruses (family geminiviridae) constitute a serious constraint to tomato production in colombia. however characterization of these new viruses had not been carried out so far. here we report a large scale survey on the distribution and genetic diversity of tomato infecting geminiviruses which are affecting mayor growing area of this crop in the country. viruses were detected by pcr using universal primers for members of genus begomovirus. the rflp analysis of pcr-amplified fragments showed individual and mixed infections of several geminiviruses in many of the samples. pcr-amplified fragments were cloned and sequenced. based on sequence comparations and phylogenetic analysis, the colombian geminivirus isolates were new world bipartite geminiviruses showing close relationship with pymv and tovev. by means of bioinformatic analysis of cis-acting elements (iterons) involved in dna replication of rep gene of colombian geminivirus isolates was possible to postulate possible pseudorecombinación events that could occur between them but also confirm the occurrence of mixed infections.
Detección, identificación y localización geográfica de Begomovirus que afectan al tomate en Colombia
Vaca-Vaca Juan Carlos,Betancurt-Pérez Jhon Fredy,López-López Karina
Revista Colombiana de Biotecnología , 2011,
Abstract: Detection, identification and geographical localization of tomato-infecting Begomovirus in Colombia Título corto: Begomovirus que afectan tomate en Colombia Resumen En la actualidad, los Begomovirus (Familia Geminiviridae) se han convertido en la mayor amenaza para los cultivos de interés agrícola ubicados en las zonas tropicales y templadas del planeta. Estos virus son transmitidos por la mosca blanca Bemisia tabaci, la cual en los últimos a os en Colombia ha tenido un aumento significativo en sus poblaciones y se ha asociado con la aparición de síntomas virales en cultivos de tomate. Muestras de tomate con síntomas virales típicos fueron recolectadas en las cinco principales zonas productoras de esta solanácea en el país. Los Begomovirus fueron detectados por medio de la técnica de hibridación de ácidos nucleicos tipo Dot blot así como por medio de la reacción en cadena de la polimerasa (PCR) en todas las muestras colectadas. Con la excepción de un reporte previo en el Valle del Cauca, este es el primer reporte de Begomovirus afectando cultivos de tomate en los departamentos de Antioquia, Santander, Boyacá y Cundinamarca. Asimismo, es la primera vez que se informa sobre Begomovirus que afectan cultivos de tomate localizados por encima de 1500 msnm en Colombia. Palabras clave: Begomovirus, Bemisia tabaci, tomate, Dot blot, PCR. Abstract The begomoviruses (Family Geminiviridae) have recently emerged as Key words: Begomovirus, Bemisia tabaci, tomato, Dot blot, PCR. samples with typical Begomovirus symptoms were collected in five different departments, comprising the mayor tomato growing areas of the country. Begomovirus were detected by Polymerase Chain Reaction (PCR) or Dot Blot Hybridization in all tomato samples collected in whole tomato growing areas of the country. With exception for Valle del Cauca, this is the first report of tomato-infecting Begomovirus in Antioquia, Santander, Boyacá and Cundinamarca departments. Also this is the first report of tomato-infecting Begomovirus crops located above 1500 masl in Colombia.
Distribución y diversidad genética de Begomovirus que infectan tomate (Solanum lycopersicum L) en Colombia
Vaca-Vaca Juan Carlos,Betancur-Pérez Jhon Fredy,López-López Karina
Revista Colombiana de Biotecnología , 2012,
Abstract: Título en ingles: Distribution and genetic diversity of tomato –infecting Begomovirus in Colombia Resumen: Las enfermedades causadas por los begomovirus, (Familia Geminiviridae) constituyen una serie limitante para la producción del tomate en Colombia. Sin embargo, la caracterización de estos virus no ha sido realizada al momento. Aquí presentamos los resultados de un muestreo a nivel nacional que buscaba conocer la distribución y diversidad genética de los geminivirus que están afectando el cultivo de tomate en Colombia. Los virus fueron detectados mediante PCR, empleando primer universales específicos para el género Begomovirus. Los fragmentos amplificados por PCR fueron sometidos a un análisis tipo RFLP cuyos resultados evidenciaron presencia de infecciones mixtas e individuales de geminivirus en la mayoría de las muestras recolectadas en campo. Los fragmentos amplificados por PCR fueron clonados y secuenciados. El análisis de secuencia y filogenético mostró que los aislados begomovirales colombianos eran gemininivirus bipartitas típicos del Hemisferio Occidental y que algunos eran variantes de PYMV y otros de ToTEV. Mediante el análisis de los elementos cis-regulatorios (iterones) presentes en el promotor del gen de la proteína asociada a replicación (Rep) de los begomovirus aislados es posible postular eventos de pseudorecombinación que podrían suceder entre ellos durante la ocurrencia de infecciones mixtas en tomate. Palabras claves: Geminivirus; Solanum licopersicum; PCR-RFLP; infecciones mixtas. Abstract: Diseased caused by begomoviruses (Family Geminiviridae) constitute a serious constraint to tomato production in Colombia. However characterization of these new viruses had not been carried out so far. Here we report a large scale survey on the distribution and genetic diversity of tomato infecting geminiviruses which are affecting mayor growing area of this crop in the country. Viruses were detected by PCR using universal primers for members of genus Begomovirus. The RFLP analysis of PCR-amplified fragments showed individual and mixed infections of several geminiviruses in many of the samples. PCR-amplified fragments were cloned and sequenced. Based on sequence comparations and phylogenetic analysis, the Colombian geminivirus isolates were new world bipartite geminiviruses showing close relationship with PYMV and ToVEV. By means of bioinformatic analysis of cis-acting elements (iterons) involved in DNA replication of rep gene of Colombian geminivirus isolates was possible to postulate possible pseudorecombinación events that could occur betwee
Búsqueda de genes de resistencia al pasador del fruto en poblaciones segregantes de tomate
Rodríguez Mora,Diana Milena; Betancur-Pérez,Jhon Fredy; Vallejo Cabrera,Franco Alirio; Vaca-Vaca,Juan Carlos; López-López,Karina;
Acta Agronómica , 2010,
Abstract: breeding strategies for resistance to fruit borer neoleucinodes elegantalis in tomato solanum lycopersicum k l. have relied on traditional methods through genetic introgression by backcrossing. however, this process is slow and expensive. the cdna-aflp molecular technique was used to identify genes involved on fruit borer resistance in tomato segregating populations, the evaluated materials of tomato were: unapal maravilla (susceptible to fruit borer) , s. habrochaites var. glabratum, acc. pi 134418 (resistant to fruit borer), and three interspecific backcross produced by crossbreeding between those two species. from these plant materials, total rna was extracted and cdna-aflp technique was implemented. from the preamplified cdna, different combinations of primers: +1+1, +3+3 and their derivates were evaluated. the combination that showed better amplified was +1+1 for a total of 37 polymorphic bands and 9 fragments type expressed sequence target was isolated, which could explain the resistance of tomato to fruit borer.
La estimulación eléctrica del sistema nervioso central con finalidad analgésica
López-López,J. A.;
Revista de la Sociedad Espa?ola del Dolor , 2006,
Abstract: different procedures using electricity for pain control (mainly neuropathic) are described in this review. history of the so-called utility of electricity for pain control, describing the early attempts at the xviii century and their evolution. spinal cord stimulation: how does it works, patient?s selection, implant techniques, how to do the different procedures and complications and their management are described. brain stimulation: the same scheme as above, concerning both deep brain stimulation and motor cortex stimulation.
La estimulación eléctrica del sistema nervioso central con finalidad analgésica The electrical stimulation of the central nervous system for pain control
J. A. López-López
Revista de la Sociedad Espa?ola del Dolor , 2006,
Abstract: Esta revisión pretende orientar al lector sobre los procedimientos empleados para el control del dolor crónico, preferentemente neuropático, por medio de la electricidad. Historia de la utilización de la electricidad con finalidad analgésica, con descripción de los usos primitivos en el siglo XVIII y su evolución. Estimulación eléctrica medular: Se describen los mecanismos de acción, la selección de los pacientes, las técnicas de implante, la forma de realización de los diferentes procedimientos y sus complicaciones y el manejo de las mismas. Estimulación cerebral: Se sigue el mismo esquema que en el apartado anterior, diferenciándose en dos apartados: la estimulación cerebral profunda y la estimulación de la corteza prefrontal. Different procedures using electricity for pain control (mainly neuropathic) are described in this review. History of the so-called utility of electricity for pain control, describing the early attempts at the XVIII century and their evolution. Spinal cord stimulation: How does it works, patient’s selection, implant techniques, how to do the different procedures and complications and their management are described. Brain stimulation: The same scheme as above, concerning both Deep Brain Stimulation and Motor Cortex Stimulation.
Increasing Water Use Efficiency in Husk Tomato (Physalis ixocarpa Brot) Production in Tabasco, Mexico with Improved Irrigation Water Management  [PDF]
Rutilo López-López, Ignacio Sánchez Cohen, Marco Antonio InzunzaIbarra, Andrés Fierro álvarez, Gerardo Esquivel Arriaga
Journal of Water Resource and Protection (JWARP) , 2014, DOI: 10.4236/jwarp.2014.613114
Abstract: Husk tomato production technology was developed utilizing plastic sheeting and drip irrigation based on the results of reference evapotranspiration and crop coefficients (Kc) proposed at different phenological stages. The objectives of this study were as follows: 1) to evaluate the effect of plastic sheeting on the yield of husk tomato fruit with drip irrigation based on crop evapotranspiration; 2) to validate the use of drip irrigation technology and plastic sheeting in husk tomato production using demonstration plots; and 3) to determine the profitability and economic viability of the product in the domestic market. The proposed Kc values were 0.25, 0.71 and 0.56 in the initial, intermediate and final stage of crop development, respectively. Irrigation rates ranged from 2.5 to 6 mm·day-1, which was equivalent to an irrigation time of 0.6 to 2.5 hr. Soil moisture tension was monitored with tensiometers at depths of 15 and 30 cm in a loamy eutricfluvisol. The use of plastic sheeting increased husk tomato yield by 56.2% and water productivity by 63.5% under drip irrigation conditions when basing irrigation levels on crop evapotranspiration. Soil matrix potential varied between depths of 15 and 30 cm based on irrigation or rainfall amounts and the use of plastic sheeting with values being higher when the plastic sheeting was used. The husk tomato fruit yield in validation plots was 46 and 54.6 t·ha-1 with water use efficiencies ranging from 16.1 to 19.1 kg·m-3. These results exceeded the national average of 14 t·ha-1 and water use efficiency of 2.54 kg·m-3. With this new technology, a cost benefit ratio of 3.6 is obtained, which represents an economically profitable margin for farmers in the region.
Gill and Liver Histopathology in Goodea atripinnis Jordan, Related to Oxidative Stress in Yuriria Lake, Mexico
Ruiz-Picos,R; López-López,E;
International Journal of Morphology , 2012, DOI: 10.4067/S0717-95022012000300060
Abstract: in aquatic ecosystems, the complex mixture of pollutants may mediate the formation of free radicals and cause oxidative damage to the biota. yuriria lake (a ramsar site in central mexico) receives input of wastewater from its tributaries, agricultural runoff, and municipal discharge. we studied the lipid peroxidation (lpo), antioxidant enzymes (superoxide dismutase (sod), catalase (cat), and glutathione peroxidase (gpx), and histopathology of gill and liver of the native fish goodea atripinnis in yuriria lake. results were compared to a control group of fish cultivated in the laboratory. lpo, sod, and cat showed no significant differences compared to controls, but gpx showed greater and significant differences in both tissues. three class sizes were identified; organisms of classes i and ii had slight vasocongestion in the liver as compared to controls. hepatocytes of class iii showed cytoplasmic vacuolization, cellular disorganization, and the liver showed marked fibrosis compared to controls. gills of controls and classes i and ii showed no damage in gill filaments. tissue damage in class iii included hypertrophy, loss of the typical morphology, and edema in the gill filaments. the longer exposure of older organisms to yuriria lake conditions may have resulted in their poorer health condition.
Terrorism: Two Complementary Approaches
Wilson López-López,Claudia Pineda
Terapia Psicológica , 2011,
Abstract: A raíz de los atentados terroristas ocurridos en Noruega el 22 de julio de 2011, el presente artículo hace una revisión de aproximaciones que sobre el fenómeno del terrorismo han sido planteadas desde la psicología social y la psicología clínica. Desde las dos disciplinas de la psicología se identifican algunos factores de riesgo de tipo psicosocial e individual. Sin embargo en casos como el de Noruega, hay una clara demanda a la comprensión y explicación de las acciones violentas, desde áreas diversas (en niveles macrosociales e individuales) complementarias de la psicología, que puedan contribuir a la prevención y/o detección de potenciales atentados que desestabilicen o pretendan manipular comportamientos sociales utilizando medios violentos, extremistas, que lesionan los derechos humanos fundamentales.
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