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Search Results: 1 - 10 of 225279 matches for " Jeremi? Svetlana R. "
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HPLC, UV-Vis and NMR spectroscopic and DFT characterization of purpurin isolated from Rubia tinctorum L.
Markovi? Zoran S.,Manojlovi? Nedeljko T.,JeremiSvetlana R.,?ivi? Miroslav
Hemijska Industrija , 2013, DOI: 10.2298/hemind120419058m
Abstract: Purpurin (1,2,4-trihidroxyanthraquinone), a naurally occuring anthraquinone pigment present in the root of Rubia tinctorum (Mull. Arg.) Zahlbr. was isolated from the plant, purified and characterized by HPLC chromatography, UV-Vis and NMR spectroscopy. The geometries of the purpurin conformers were optimized using the B3LYP/6-311+G(d,p) level of theory. It was found that all conformations have similar energies and that the corresponding purpurin radicals are planar. In spite of their planarity, there is no significant electron delocalization over the A and C rings. The antioxidant properties of purpurin were investigated using the colorimetric assay as Trolox-equivalent antioxidant capacity, and theoretical BDE (Bond Dissociation Enthalpy). Both methods revealed that purpurin has strong antioxidant capacity.
Koi herpesvirus disease in carp
JeremiSvetlana,Radosavljevi? Vladimir
Veterinarski Glasnik , 2007, DOI: 10.2298/vetgl0702053j
Abstract: A disease in the koi carp (Cyprinus carpio koi) and the common carp (Cyprinus carpio carpio), caused by the herpesvirus and accompanied by a high mortality rate, has spread across numerous fish ponds all over the world since 1998, resulting in massive mortality and significant financial losses. The herpesvirus-like virus, called the koi herpesvirus (KHV) has been isolated and identified from the koi and the common carp in the course of the incidences of massive mortalities. The first appearance of a disease with a high mortality in the common and the koi carp caused by the koi herpesvirus (KHV) was described in 1998 in Israel and the United States of America (USA). Since that time, a large number of cases of outbreaks of this disease have been confirmed throughout the world, including the USA, Israel, and a large number of European countries. The deaths occurred seasonally, in late spring or early autumn, when the water temperature was from 18-28oC. The most important factor of the environment that affects the occurrence and gravity of this disease is the water temperature. This disease is currently considered one of the factors that present the biggest threat to populations of the common and the koi carp. Diseased fish are disoriented, their movements uncoordinated, their breathing rapid, gills swollen, and they have local skin lesions. The virus was isolated from tissue of diseased fish and cultivated on a KF-1 (koi fin cells) cell line. Electronic microscopy examinations revealed virus identical viral particles of the Herpesviridae family. Analyses of the virion polypeptide and DNA established differences between the KHV and the previously known herpesvirus of the Cyprinida family, Herpesvirus cyprini (CHV), and the virus of the channel catfish (Channel catfish virus - CCV). In the years 2004 and 2005, high mortality was established among one-year and two-year carp fry on three fish ponds. At two ponds, the deaths occurred among one year and two-year carp fry during the spring period, when the water temperature was over 18oC. During the autumn period, mortality was recorded among one-year carp fry at water temperatures above 23oC. On the grounds of the determined pathomorphological changes and the high mortality during the period of the year when the temperature was above 18oC, we suspect that KHV is also present in fish ponds in Serbia, even though the virus itself has not been isolated. The objective of this work is briefly to present the relevant data on this disease which is inflicting significant losses to carp production, to show the current di
Sistemic Citrobacter Freundii infection in carp
JeremiSvetlana,Jaki?-Dimi? Dobrila
Veterinarski Glasnik , 2003, DOI: 10.2298/vetgl0302003j
Abstract: The paper describes ta clinical disease in carp fish species which indicated a typical acute bacterial septicemia caused by the gram-negative bacteria Citrobacter freundii. An unusual mortality of carp fish species was observed during low water temperatures at the beginning of the year. A total of 39 samples were taken for laboratory examinations from diseased fish which had become darker, with expressed exophthalmus bleedings on the skin and eyes and 10 samples from clinically healthy fish. Part of the altered organs gills, parenchymatous organs and intestines were used for isolating the causes. The usal methods of bacteriological examinations were used for the investigations. Cultures were identified by examining key phenotype markings and using an Api 20E system as Citrobacter freundii. Other parts of parenchymatous organs and intestines were fixed in 10% formaline prepared according to the standard histological method. Pathohistological examinations established inflammatory and necrotic changes in internal organs. We succeeded in reproducing the disease with an artificial infection using a pure culture of Citrobacter freundii. This is the first published report on establishing Citrobacter freundii as the cause of carp diseases in Serbia.
Citrobacter freundii as a cause of disease in fish
JeremiSvetlana,Jaki?-Dimi? Dobrila,Veljovi? Lj.
Acta Veterinaria , 2003, DOI: 10.2298/avb0306399j
Abstract: The paper describes an illness of one-year rainbow trout fry that was characterized by gastroenteritis and progressively high mortality, but which did not indicate a typical bacterial infection; and a clinical illness of cyprinids that indicated typical acute bacterial septicemia caused by Gram-negative bacteria. These diseases of rainbow trout and cyprinids were caused by the Gram-negative motile bacterium Citrobacter freundii. Cultures of Citrobacter freundii were isolated and identified on the basis of key phenotypic characters and with the aid of the Api 20 E system. Pathohistological examination confirmed inflammatory changes in the intestine of rainbow trout; and inflammatory and necrotic changes in the internal organs of cyprinids. We were able to reproduce the illness by means of artificial infection with a pure culture of Citrobacter freundii. This is the first published report confirming Citrobacter freundii as a cause of fish disease in Serbia.
Dissemination of spring viraemia of carp (SVC) in Serbia during the period 1992-2002
JeremiSvetlana,Jaki?-Dimi? Dobrila,Radosavljevi? V.
Acta Veterinaria , 2004, DOI: 10.2298/avb0404289j
Abstract: An acute contagious viral disease caused by Rhabdovirus carpio, spring viraemia of carp, was described for the first time by Fijan in 1972. Carp is the most important susceptible species in all age categories, although other species of cyprinid fish also contract the disease. As the name suggest, the disease typically appears in spring, when the water temperature increases. The disease is often complicated by the secondary appearance of bacterial and parasitic infections. The clinical symptoms include and presume gathering of infected fish near the sides of the pond, uncoordinated swimming, darkening of body color, pale gills, petechial bleeding (of the skin, eyes, gills, and fin bases), exophthalmus, and the appearance of pseudofecal expulsions from the infected anus. Pathoanatomical examination sometimes reveals only oedematous organ enlargement and intestinal inflammation. A varying degree of haemorrhaging is present in the bladder, internal organs, and fluid-containing cavities. Diagnosis is based on isolation and identification of the virus and ELISA in the laboratory. More than 1664 samples were analyzed in the course of the 10-year investigation (from 1992-2002) from different carp hatchery localities in the Republic of Serbia. Rhabdovirus carpio was isolated and identified at more than a third of the tested fish hatcheries (31,57%) infected with the spring viraemia virus. Although the disease was known earlier, it now represents an urgent problem in many European countries, and has been causing great damage in intensive and semi-intensive carp production in our country during the last few years. Therefore, special attention has been paid to it.
Rhabdovirus carpio as a causative agent of disease in rainbow trout (Oncorhynchus mykiss - Walbaum)
JeremiSvetlana,Iveti? V.,Radosavljevi? V.
Acta Veterinaria , 2006, DOI: 10.2298/avb0606553j
Abstract: High mortality of 1-year old rainbow trout occurred on a fish farm in the spring season, with clinical symptoms typical for acute septicemia. Histological examination revealed inflammatory changes and necrosis of the internal organs. The causative agent was isolated on RTG-2 and EPC cell lines and identified by serum neutralisation, enzyme-linked immunosorbent assay (ELISA) and immunofluorescence (IFAT) as Rhabdovirus carpio. Experimental infection using the virus isolated from rainbow trout caused disease in carp fry, as well. This is the first case of disease of rainbow trout caused by Rhabdovirus carpio, and the first reported isolation of Rhabdovirus carpio from rainbow trout in Serbia. Also, this is the first successful infection of carp with Rhabdovirus carpio isolated from rainbow trout.
Cyclic conjugation in benzo-annelated triphenylenes
SVETLANA JEREMI,SLAVKO RADENKOVI?,IVAN GUTMAN
Journal of the Serbian Chemical Society , 2010,
Abstract: Cyclic conjugation in benzo-annelated triphenylenes was studied by means of the energy effect (ef) and the π-electron content (EC) of the six-membered rings. A regularity that was earlier discovered in the case of acenaphthylene and fluoranthene congeners is now shown to hold also for benzo-annelated triphenylenes: Benzenoid rings that are annelated angularly with regard to the central six-membered ring Z0 of triphenylene increase the intensity of the cyclic conjugation in Z0, whereas linearly annelated benzenoid rings decrease the cyclic conjugation in Z0. The ef- and EC-values are strongly correlated, yet in a non-linear manner.
Diradical character of some fluoranthenes
SVETLANA MARKOVI?,JELENA ?UR?EVI?,SVETLANA JEREMI,IVAN GUTMAN
Journal of the Serbian Chemical Society , 2010,
Abstract: It is shown that some Kekuléan fluoranthenes are diradicals and that their ground state is a triplet. In the energetically less favorable singlet state, these hydrocarbons also exhibit pronounced diradical character. The diradical character y of the compounds under investigation was estimated using the unrestricted symmetry-broken (yPUHF) and complete active space (yNOON) methods. It was found that the yPUHF values better reproduce the diradical character of the investigated hydrocarbons. It was shown that singly occupied molecular orbital (SOMO) and SOMO-1 of a diradical structure occupy different parts of space with a small shared region, resulting in a spin density distribution over the entire molecule. The spatial diradical distribution in the singlet diradical structures was examined by inspecting the HOMOs and LUMOs for a and b spin electrons. It was shown that the a-HOMO and the b-LUMO (as well as the b-HOMO and the a-LUMO) occupy practically the same part of space. In this way, there are no unpaired electrons in a singlet diradical structure, yet two of them occupy different parts of space, thus allowing the p-electrons to delocalize.
Common fish species in polyculture with carp as cyprinid herpes virus 3 carriers
Radosavljevi? Vladimir,JeremiSvetlana,?irkovi? M.,Lako B.
Acta Veterinaria , 2012, DOI: 10.2298/avb1206675r
Abstract: Cohabitation studies with common carp were conducted to determine whether the Cyprinid Herpes Virus 3 can infect and establish a productive infection in fish species that according to available data, are not susceptible to this virus. In order to examine if other fish species can contribute to further spreading of the virus, goldfish, silver carp, grass carp, prussian carp and tench were exposed to CyHV-3 through cohabitation with infected carp without clinical symptoms. After this period they cohabitated with naive carp for two weeks and were examined for CyHV-3 by PCR. Our results showed that CyHV-3 was present in the organs of these fish species and also in organs of naive carp after two weeks in cohabitation, suggesting that CyHV-3 may cause latent infection, and also that has a potential to infect a broader host range than it was believed before. Our study adds on better understanding of CyHV-3 transmission not just in its primary host, but also suggests the importance of common fish species in polyculture with carp in the epidemiology of CyHV-3. [Projekat Ministarstva nauke Republike Srbije, br. TR31075 i br. TR-31011]
Fish diseases in carp fish ponds and implementation of health care measures
JeremiSvetlana,?irkovi? Miroslav,Jaki?-Dimi? Dobrila,Radosavljevi? Vladimir
Veterinarski Glasnik , 2005, DOI: 10.2298/vetgl0502059j
Abstract: Fish health protection is a complex and permanent measure veterinary specialists are taking in order to preserve and improve breeding and production of particular fish species and categories. The system of monitoring fish health should ensure early detection of disorders in fish health and the presence of causing agents. In order for the monitoring to be efficient it should be practiced in accordance with the specific conditions of each system and breeding venues, as well as to the specific health problem needs of different farmed fish species. The most important issue in fish diseases diagnostics is the systematic monitoring of the condition offish health. Only in such way it is possible to detect a disease on time and then determine the adequate therapeutic and other necessary measures. In dealing with the problems offish pathology in carp fishponds, the epizootical situation of disease spreading caused by different agents (viruses, bacteria, fungi and parasites) has been examined. The most frequent diseases among the farmed carps in the examined fish ponds in Vojvodina area, described in this paper were: carp pox, spring viremia of carp, carp erythrodermatitis, aeromonas and pseudomonas infections, bacterial gill disease, diseases caused by ecto- and endoparasites and gill necrosis. Based on the obtained results, modern diagnostic methods were implemented and proper prevention and successful therapy of the diseases causing the greatest loss in farmed fish populations was taken.
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