Publish in OALib Journal
APC: Only $99
In this work,
nodal-disk segments (4-6 mm in diameter × 5-6 mm in length) were
obtained from established shoot culture, resulted from disinfected tomato
seedlings, and they were suitable to induce different organogenic pathway under
the influence of specific hormonal treatment. Application of BAP (1-2.5 mg/l)
alone or in combination of 0.5 mg/l NAA resulted in induction of shoot formation.
Somatic embryogenesis was rarely appeared (6%) when relatively low
concentration of BAP (1.5 mg/l) with low concentration of IAA (0.5 mg/l IAA)
was applied. Root induction was triggered when nodal explants or shoot cuttings
were cultured on MS medium with (1 mg/l IAA, IBA or NAA) or without auxins, but
the best result was obtained when 1 mg/l IAA was used. Application of 0.5 mg/l
NAA stimulated callus formation but the best result was obtained when the three
different phytohormoes were used (0.5 mg/l 2,4-D + 1 mg/l NAA + 0.5 mg/l BAP).
These results indicated that nodal segments, as described in this protocol, can
be used as alternative to other types of explants such as cotyledon, hypocotyl
and leaf explants.
An experiment was conducted in
pots under natural conditions in alkaline calcareous soil to determine wheat (Triticum aestivum L. c.v. Atta Habib)
yield and P uptake as influenced by Arbuscular mycorrhizal fungi (AMF)
inoculation with compost prepared from fresh animal dung and rock phosphate.
Data indicated that wheat grain, shoot and roots yields increased significantly
(P ≤ 0.05) by inoculation of commercial mycorrhiza (AMF-II) and half dose of
compost. Grain yield increased by 43% and 37%, shoot by 43% and 39% and roots
yield by 51% and 45% over control of N and K fertilizers. Straw yield was
maximum as 5075 kg·ha-1 in the treatment of AMF-II inoculation with full dose of compost,
which was significantly (P ≤ 0.05) higher as 44% and 40% over control of N and
K fertilizers. Maximum and significantly (P ≤ 0.05) higher plant N and P uptake
by wheat were observed in the treatment inoculated by indigenous mycorrhiza
(AMF-I) with full dose of compost followed by the inoculation of AMF-II with
full dose of compost and SSP treatment. Maximum and significantly (P ≤ 0.05)
increased soil spores’ density of AMF by 26 spores per 20 g soil with maximum
roots infection intensity in wheat were observed by the inoculation of AMF-I
with full dose of compost. The AMF-II is slightly better than AMF-I regarding
grain, shoot and root yield, whereas AMF-I is better in N, P uptake, soil spore
density and their root infection intensity than AMF-II. Alone inoculation and
compost application increase the yield and nutrients uptake but the highest
improvement was observed with inoculation of AMF with compost. Results suggest
that inoculation of AMF with compost has potential to improve wheat yields and
plants’ P uptake under given soil conditions.