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Search Results: 1 - 10 of 53820 matches for " Jean-Yun Chang "
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Complement C1q Activates Tumor Suppressor WWOX to Induce Apoptosis in Prostate Cancer Cells
Qunying Hong, Chun-I Sze, Sing-Ru Lin, Ming-Hui Lee, Ruei-Yu He, Lori Schultz, Jean-Yun Chang, Shean-Jen Chen, Robert J. Boackle, Li-Jin Hsu, Nan-Shan Chang
PLOS ONE , 2009, DOI: 10.1371/journal.pone.0005755
Abstract: Background Tissue exudates contain low levels of serum complement proteins, and their regulatory effects on prostate cancer progression are largely unknown. We examined specific serum complement components in coordinating the activation of tumor suppressors p53 and WWOX (also named FOR or WOX1) and kinases ERK, JNK1 and STAT3 in human prostate DU145 cells. Methodology/Principal Findings DU145 cells were cultured overnight in 1% normal human serum, or in human serum depleted of an indicated complement protein. Under complement C1q- or C6-free conditions, WOX1 and ERK were mainly present in the cytoplasm without phosphorylation, whereas phosphorylated JNK1 was greatly accumulated in the nuclei. Exogenous C1q rapidly restored the WOX1 activation (with Tyr33 phosphorylation) in less than 2 hr. Without serum complement C9, p53 became activated, and hyaluronan (HA) reversed the effect. Under C6-free conditions, HA induced activation of STAT3, an enhancer of metastasis. Notably, exogenous C1q significantly induced apoptosis of WOX1-overexpressing DU145 cells, but not vehicle-expressing cells. A dominant negative and Y33R mutant of WOX1 blocked the apoptotic effect. C1q did not enhance p53-mediated apoptosis. By total internal reflection fluorescence (TIRF) microscopy, it was determined that C1q destabilized adherence of WOX1-expressing DU145 cells by partial detaching and inducing formation of clustered microvilli for focal adhesion particularly in between cells. These cells then underwent shrinkage, membrane blebbing and death. Remarkably, as determined by immunostaining, benign prostatic hyperplasia and prostate cancer were shown to have a significantly reduced expression of tissue C1q, compared to age-matched normal prostate tissues. Conclusions/Significance We conclude that complement C1q may induce apoptosis of prostate cancer cells by activating WOX1 and destabilizing cell adhesion. Downregulation of C1q enhances prostate hyperplasia and cancerous formation due to failure of WOX1 activation.
Dramatic Co-Activation of WWOX/WOX1 with CREB and NF-κB in Delayed Loss of Small Dorsal Root Ganglion Neurons upon Sciatic Nerve Transection in Rats
Meng-Yen Li,Feng-Jie Lai,Li-Jin Hsu,Chen-Peng Lo,Ching-Li Cheng,Sing-Ru Lin,Ming-Hui Lee,Jean-Yun Chang,Dudekula Subhan,Ming-Shu Tsai,Chun-I Sze,Subbiah Pugazhenthi,Nan-Shan Chang,Shur-Tzu Chen
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0007820
Abstract: Tumor suppressor WOX1 (also named WWOX or FOR) is known to participate in neuronal apoptosis in vivo. Here, we investigated the functional role of WOX1 and transcription factors in the delayed loss of axotomized neurons in dorsal root ganglia (DRG) in rats.
Increased IgG4-Positive Plasma Cells in Granulomatosis with Polyangiitis: A Diagnostic Pitfall of IgG4-Related Disease
Sing Yun Chang,Karina Keogh,Jean E. Lewis,Jay H. Ryu,Eunhee S. Yi
International Journal of Rheumatology , 2012, DOI: 10.1155/2012/121702
Abstract: Granulomatosis with polyangiitis (Wegener’s) (GPA) may mimic IgG4-related disease (IgG4-RD) on histologic examination of some biopsies, especially those from head and neck sites. IgG4 immunostain is often performed in this context for differential diagnosis with IgG4-RD. However, the prevalence of IgG4+ cells in GPA has not been explored. We examined the IgG4+ cells in 26 cases confirmed as GPA by a thorough clinical and pathologic assessment. Twenty-six biopsies consisted of 14 sinonasal/oral cavity/nasopharynx, 7 orbit/periorbital, 3 lung/pleura, 1 iliac fossa/kidney, and 1 dura specimens. Eight of 26 (31%) biopsies revealed increased IgG4+ cells (>30/HPF and >40% in IgG4+/IgG+ ratio). The IgG4+ cells and IgG4+/IgG+ ratio ranged 37–137/hpf and 44–83%, respectively. Eight biopsies with increased IgG4+ cells were from sinonasal or orbital/periorbital sites. In conclusion, increased IgG4+ cells are not uncommonly seen in sinonasal or orbital/periorbital biopsies of GPA, which could pose as a diagnostic pitfall. 1. Introduction Granulomatosis with polyangiitis (Wegener’s) (GPA) is an immune-mediated systemic necrotizing vasculitis often affecting the upper respiratory tract, lung, and kidney [1]. Involvement of GPA is limited to the upper respiratory tract and/or the lung in some cases although virtually anybody site can be involved in GPA such as the eye, skin, joints, heart, and the central nervous system [2]. Necrotizing vasculitis and irregular basophilic parenchymal necrosis with associated palisading granuloma comprise the main histologic characteristics of GPA. Also, neutrophilic microabscesses and fibrosis are commonly found in a background mixed inflammatory infiltrate composed of neutrophils, lymphocytes, plasma cells, multinucleated giant cells, and macrophages [1, 2]. On histologic examination, GPA can mimic IgG4-related disease (IgG4-RD) since the inflammatory background in GPA may be rich in plasma cells and accompanied by fibrosis and/or obliterated blood vessels as in IgG4-RD [1, 3, 4]. Some biopsies of GPA cases (especially from the upper respiratory tract and orbit) may lack classic morphologic features such as necrotizing vasculitis, parenchymal necrosis, and palisading granuloma [5, 6]. IgG4 immunostain is now often performed in this context for evaluating the possibility of IgG4-RD. However, the prevalence of IgG4-positive (IgG4+) cells in GPA has not been widely reported in the literature. Therefore, we sought to assess the prevalence of IgG4+ cells in GPA cases that have been confirmed by a thorough clinical and pathologic
Electrical Conductivity and Current--Voltage Characteristics of Individual Conducting Polymer PEDOT Nanowires

LONG Yun-Ze,DUVAIL Jean-Luc,CHEN Zhao-Jia,JIN Ai-Zi,GU Chang-Zhi,

中国物理快报 , 2008,
Functional Module Connectivity Map (FMCM): A Framework for Searching Repurposed Drug Compounds for Systems Treatment of Cancer and an Application to Colorectal Adenocarcinoma
Feng-Hsiang Chung, Yun-Ru Chiang, Ai-Lun Tseng, Yung-Chuan Sung, Jean Lu, Min-Chang Huang, Nianhan Ma, Hoong-Chien Lee
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0086299
Abstract: Drug repurposing has become an increasingly attractive approach to drug development owing to the ever-growing cost of new drug discovery and frequent withdrawal of successful drugs caused by side effect issues. Here, we devised Functional Module Connectivity Map (FMCM) for the discovery of repurposed drug compounds for systems treatment of complex diseases, and applied it to colorectal adenocarcinoma. FMCM used multiple functional gene modules to query the Connectivity Map (CMap). The functional modules were built around hub genes identified, through a gene selection by trend-of-disease-progression (GSToP) procedure, from condition-specific gene-gene interaction networks constructed from sets of cohort gene expression microarrays. The candidate drug compounds were restricted to drugs exhibiting predicted minimal intracellular harmful side effects. We tested FMCM against the common practice of selecting drugs using a genomic signature represented by a single set of individual genes to query CMap (IGCM), and found FMCM to have higher robustness, accuracy, specificity, and reproducibility in identifying known anti-cancer agents. Among the 46 drug candidates selected by FMCM for colorectal adenocarcinoma treatment, 65% had literature support for association with anti-cancer activities, and 60% of the drugs predicted to have harmful effects on cancer had been reported to be associated with carcinogens/immune suppressors. Compounds were formed from the selected drug candidates where in each compound the component drugs collectively were beneficial to all the functional modules while no single component drug was harmful to any of the modules. In cell viability tests, we identified four candidate drugs: GW-8510, etacrynic acid, ginkgolide A, and 6-azathymine, as having high inhibitory activities against cancer cells. Through microarray experiments we confirmed the novel functional links predicted for three candidate drugs: phenoxybenzamine (broad effects), GW-8510 (cell cycle), and imipenem (immune system). We believe FMCM can be usefully applied to repurposed drug discovery for systems treatment of other types of cancer and other complex diseases.
菌物学报 , 1985,
Abstract: Ⅰ.Ancient Literature ReviewThe edibility and medicinal use of fungi were known to Chinese ancestors long,long ago.Our fathers recognized and used them as food,as medicine,in agriculture,in industry,for fermentation,etc.Fungi such as common mushrooms,wood ear,jellyfungus(Tremella fusiformis)have been used for food;china root(Poria coccus),ca-
Design of an 8192-point Sequential I/O FFT Chip
Yun-Nan Chang
Lecture Notes in Engineering and Computer Science , 2012,
Coordinates, retracts and automorphisms
Yun-Chang Li
Mathematics , 2012,
Abstract: Let $K$ be a field of characteristic zero, $K[x,y]$ be the polynomial ring in two variables. Let $\phi=(f, g)$ be an endomorphism of $K[x,y]$. It is proved that if $\phi$ maps each coordinate to a generator of some proper retract, then it is an automorphism. As a corollary, the retract preserving problem is solved for both polynomial ring over $K$ and free algebra over an arbitrary field when $n=2$.
菌物学报 , 1985,
Consumer Lifestyle Matters: Evidence from Gray Markets in China  [PDF]
Weining Liu, Lan-Yun Chang, Jing-Ru Lin
Journal of Service Science and Management (JSSM) , 2012, DOI: 10.4236/jssm.2012.52024
Abstract: Consumers are at the central point of marketing. However, while existential research is devoted to understanding gray market, little attention is given to the consumer’s view of gray market. This study attempts to take the perspective of consumers to address the gray market issue. Lifestyles of the target customers of trademark holders are proposed for trademark holders to retain existing customers while simultaneously attracting new customers from the gray market. We perform Cluster analysis to identify different customer groups by using “lifestyle” as a market segmentation variable. ANOVA, Scheffe test and regression analyses are then employed to test the proposed hypotheses. Analytical results reveal that the different customer groups exhibit particular lifestyle features, different perceptions of gray product quality as well as different purchase intentions.
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