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Search Results: 1 - 10 of 28732 matches for " Jean Pierre Baudoin "
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Determination of the Genetic Structure of the Oleaginous Lagenaria siceraria of the Nangui Abrogoua University Germplasm Collection  [PDF]
Ahou Anique Gbotto, Kouamé Kevin Koffi, Jean-Pierre Baudoin, Irié Arsène Zoro Bi
American Journal of Plant Sciences (AJPS) , 2015, DOI: 10.4236/ajps.2015.619314
Abstract: Thirty accessions of Lagenaria siceraria from the Nangui Abrogoua University germplasm collection were analyzed using three microsatellite markers. The average Polymorphism Information Content (PIC) value was 0.61. The average observed heterozygosity value (H0 = 0.631) compared to the average expected heterozygosity value (He = 0.645) did not show significant differences in the selected accessions, which suggested random mating in the set of accessions. Within accession inbreeding estimate (FIS), was 40% and was not significantly different from zero. The reduction of heterozygotes was likely the result of presence of null alleles. Analysis of Molecular Variance (AMOVA) within and among 30 accessions of L. siceraria revealed that 39% of the total variation resides among accessions and 61% within accessions. Accession structuring pattern derived from Bayesian clustering analysis revealed two clusters. Based on the genetic structure of the accessions analyzed, a sampling strategy to collect and conserve genetic resources of L. siceraria was suggested.
La collection de base des espèces sauvages de Phaseolus et Vigna : historique, gestion et conservation
Thierry Vanderborght,Jean-Pierre Baudoin
Biotechnologie, Agronomie, Société et Environnement , 1998,
Abstract: The base collection of wild species of Phaseolus and Vigna: history, management and conservation.The National Botanic Garden of Belgium ensures the management of a base collection of botanical and wild forms in the tribe Phaseoleae and the sub-tribe Phaseolinae. The main objective is to conserve on a long terni basic the largest possible genetic diversity through seed semples stored at - 20°C. The collection provided the basic material for the investigations conducted at the University Faculty of Agricultural Sciences of Gembloux in fields as diverse as taxonomy, genome analysis, definition of genetic réservoirs, agronomie and chemical evaluations, interspecific hybridization and plant breeding. The results have allowed to becter understand the organization of genetic diversity in the studied plant material and to highlight the wealthy genetic potentiel of the collection. The latter should be preserved and valorized for the genetic improvement of food legumes, in particular within the two genera Phaseolus and Vigna.
Etude comparative du développement de l'ovule chez deux espèces de Phaseolus : P. polyanthus et P. vulgaris
Thierry Vanderborght,Jean-Pierre Baudoin
Biotechnologie, Agronomie, Société et Environnement , 1998,
Abstract: The base collection of wild species of Phaseolus and Vigna: history, management and conservation.The National Botanic Garden of Belgium ensures the management of a base collection of botanical and wild forms in the tribe Phaseoleae and the sub-tribe Phaseolinae. The main objective is to conserve on a long terni basic the largest possible genetic diversity through seed semples stored at - 20°C. The collection provided the basic material for the investigations conducted at the University Faculty of Agricultural Sciences of Gembloux in fields as diverse as taxonomy, genome analysis, definition of genetic réservoirs, agronomie and chemical evaluations, interspecific hybridization and plant breeding. The results have allowed to becter understand the organization of genetic diversity in the studied plant material and to highlight the wealthy genetic potentiel of the collection. The latter should be preserved and valorized for the genetic improvement of food legumes, in particular within the two genera Phaseolus and Vigna.
Medium effects on micropropagation and genetic stability of Citrullus lanatus oleaginous type  [PDF]
Yah Gwladys Gnamien, Irié Arsène Zoro Bi, Yatty Justin Kouadio, Yves Brostaux, Jean-Pierre Baudoin
Agricultural Sciences (AS) , 2013, DOI: 10.4236/as.2013.47A005
Abstract:

To regenerate adventitious shoots from the cotyledon proximal parts of Citrullus lanatus (Thunb.) Matsum. and Nakai ssp. mucosospermus (Fursa) oleaginous type, different concentrations of MS mineral elements, sucrose, 6-benzylaminopurine (BAP) and agar were tested. Shoot induction proved to depend on the interaction between levels of sucrose, BAP and MS mineral elements in the medium. The medium containing 3/2 strength of MS mineral elements, 35 g/l sucrose and 1 mg/l BAP solidified with 6 g/l agar allowed the production of numerous shoots without a callus phase. After 3 weeks of culture, 76.7% of the cotyledon proximal parts induced shoots with an average of 12.26 shoots per explant and a mean shoot length of 17.13 mm. The induced shoots were directly rooted and thus complete plants ready for acclimatization were obtained using a two steps procedure. Depending on the genotype, the shoot induction from cotyledon proximal parts ranged from 54% to 96%. Rooted plantlets were acclimatized and transferred to field, where they grew well, developed flowers and fruits like seeded plants. The assessment of the genetic stability of the in-vitro-regenerated plantlets by means of an Amplified Fragment Length Polymorphism (AFLP) analysis with the combination of 5 primers revealed no differences between regenerated plantlets and mother plants.

The impact of local extinction on genetic structure of wild populations of lima beans (Phaseolus lunatus) in the Central Valley of Costa Rica: consequences for the conservation of plant genetic resources
Daniel Barrantes,Gabriel Macaya,Luigi Guarino,Jean Pierre Baudoin
Revista de Biología Tropical , 2008,
Abstract: Plant populations may experience local extinction and at the same time new populations may appear in nearby suitable locations. Species may also colonize the same site on multiple occasions. Here, we examined the impact of local extinction and recolonization on the genetic structure of wild populations of lima beans (Phaseolus lunatus) in the Central valley of Costa Rica. We compared genetic diversity from the samples taken from the populations before and after extinction at 13 locations using microsatellite markers. Locations were classified according to the occurrence of extinction episodes during the previous five years into three groups: 1) populations that experienced extinction for more than one year, and were later recolonized (recolonized), 2) populations that did not experience local extinction (control), and 3) populations that did not experience local extinction during the study, but were cut to experimentally simulate extinction (experimental). Our data did not show a clear tendency in variation in allele frequencies, expected heterozygosity, and effective number of alleles within and between groups of populations. However, we found that the level of genetic differentiation between samples collected at different times at the same location was different in the three groups of populations. Recolonized locations showed the highest level of genetic differentiation (mean Fst= 0.2769), followed by control locations (mean Fst= 0.0576) and experimental locations (mean Fst= 0.0189). Similar findings were observed for Nei’s genetic distance between samples (di,j= 0.1786, 0.0400, and 0.0037, respectively). Our results indicate that genetic change in lima beans depends on the duration and frequency of local extinction episodes. These findings also showed that control populations are not in equilibrium. Implications of these results for the establishment of conservation strategies of genetic resources of lima beans are discussed. Rev. Biol. Trop. 56 (3): 1023-1041. Epub 2008 September 30. Las poblaciones de plantas pueden experimentar extinción local, y al mismo tiempo, pueden surgir a sus alrededores nuevas poblaciones. Algunas especies pueden colonizar el mismo sitio en múltiples ocasiones. Aquí examinamos el impacto de la extinción local y recolonización en la estructura genética de poblaciones silvestres del frijol lima (Phaseolus lunatus) en el valle Central de Costa Rica. Comparamos la diversidad genética de muestras tomadas en poblaciones, antes y después de la extinción, en 13 sitios, usando marcadores de microsatélite. Según los episodios de exti
Exploitation of trispecific hybrids to introgress the glandless seed and glanded plant trait of Gossypium sturtianum Willis into G. hirsutum L.
Guy Mergeai,Jean-Pierre Baudoin,Irié Vroh Bi
Biotechnologie, Agronomie, Société et Environnement , 1997,
Abstract: Two différent trispecific hybrids were developed in order to introgress the "glandless seed-glanded plant" trait of Gossypium sturtianum Will. (2n = 2x = 26, CI genome) into the main cultivated cotton species (Gossypium hirsutum L., 2n = 4x = 52, (AD), genome) using either Gossypium raimondii Ulb. (2n = 2x = 26, D5 genome) or Gossypium thurberi Tor. (2n = 2x = 26, Dl genome) as bridge species. Both trispecific hybrids were backcrossed with two G. hirsutum varieties (C2 and NC8) originating from Zaire. Observation of the trispecific seeds pointed out the incomplets expression of the seed gossypol glands répressive mechanism of G. sturtianum when its chromosomes set is confronted with the D genome. The glandless trait was expressed in a rather high proportion of the BC, seeds: 6 out of 41. Only one of the six BC, glandless seeds gave riss to a viable glanded plant. Cytogenetic observations of both trispecific hybrids and of the introgressed plant confirmed the soundness of the introgression strategy followed. All these plants were euploid (2n = 4x = 52) and showed high frequencies of multivalent and chiasma formations at métaphase I indicating important genetic material exchanges. All the plants issued from nearly totally glandless seeds will be used in a backcrossing program with G. hirsutum to produce commercial varieties of upland cotton expressing the "glandless seed-glanded plant" trait.
Embryogenèse précoce comparative lors des croisements entre Phaseolus coccineus L. et Phaseolus vulgaris L.
Pamphile Nguema Ndoutoumou,André Toussaint,Jean-Pierre Baudoin
Biotechnologie, Agronomie, Société et Environnement , 2007,
Abstract: Comparative early embryogenesis in crossings between Phaseolus coccineus L. and Phaseolus vulgaris L. Theinterspecifi c hybridization between Phaseolus coccineus L. and Phaseolus vulgaris L. is useful for the genetic improvementof the common bean. The use of the P. vulgaris cytoplasm for such hybridizations leads usually to a rather fast return tothe maternal form in the subsequent generations. When P. vulgaris is the pollinator, crosses result in early embryo abortion(globular or heart-shaped embryos). A competition between the endosperm and the embryo, on the one hand, and between thesuspensor and the embryo, on the other hand, could generate diffi culties of feeding young embryos. Histological sections usingthe 2-Hydroxyethyl Methacrylate (HEMA) resin method on the embryos of P. coccineus (NI16) and P. vulgaris (NI637 andX707) genotypes, as well as on their genotypic combination (NI16 × NI637 and NI16 × X707 and reciprocal crosses) enableus to explain partially these abortion cases. Observations concern embryos from 3 to 6 days after pollination (DAP). Embryodevelopment (suspensor and embryo proper) of the hybrids is slower than that of the parents whatever the crossing. Ingrowthsof suspensor basal cells observed when P. coccineus is the maternal parent are characteristic of the presence of the cytoplasmof these species. Endothelium deterioration (or proliferation) in hybrid embryos would rather be related to the degree reachedby the process of abortion in the embryo concerned.
The impact of local extinction on genetic structure of wild populations of lima beans (Phaseolus lunatus) in the Central Valley of Costa Rica: consequences for the conservation of plant genetic resources
Barrantes,Daniel; Macaya,Gabriel; Guarino,Luigi; Baudoin,Jean Pierre; Rocha,Oscar J;
Revista de Biología Tropical , 2008,
Abstract: plant populations may experience local extinction and at the same time new populations may appear in nearby suitable locations. species may also colonize the same site on multiple occasions. here, we examined the impact of local extinction and recolonization on the genetic structure of wild populations of lima beans (phaseolus lunatus) in the central valley of costa rica. we compared genetic diversity from the samples taken from the populations before and after extinction at 13 locations using microsatellite markers. locations were classified according to the occurrence of extinction episodes during the previous five years into three groups: 1) populations that experienced extinction for more than one year, and were later recolonized (recolonized), 2) populations that did not experience local extinction (control), and 3) populations that did not experience local extinction during the study, but were cut to experimentally simulate extinction (experimental). our data did not show a clear tendency in variation in allele frequencies, expected heterozygosity, and effective number of alleles within and between groups of populations. however, we found that the level of genetic differentiation between samples collected at different times at the same location was different in the three groups of populations. recolonized locations showed the highest level of genetic differentiation (mean fst= 0.2769), followed by control locations (mean fst= 0.0576) and experimental locations (mean fst= 0.0189). similar findings were observed for nei?s genetic distance between samples (di,j= 0.1786, 0.0400, and 0.0037, respectively). our results indicate that genetic change in lima beans depends on the duration and frequency of local extinction episodes. these findings also showed that control populations are not in equilibrium. implications of these results for the establishment of conservation strategies of genetic resources of lima beans are discussed. rev. biol. trop. 56 (3): 1023-1041. e
EVALUACIóN AGROMORFOLóGICA Y CARACTERIZACIóN MOLECULAR DE LA U A (PHASEOlUS VULGARIS L.) AGROMORPHOLOGICAL EVALUATION AND MOLECULAR CHARACTERIZATION OF POPPING BEAN (PHASEOLUS VULGARIS L.)
José Cruz Balarezo,Félix Camarena Mayta,Jean Pierre Baudoin,Amelia Huaringa Joaquín
IDESIA , 2009,
Abstract: La u a es una especie originaria de los Andes centrales, sus granos presentan alto contenido de proteínas y es consumida tostada. Se estudiaron 24 entradas, con el objetivo de determinar el nivel de duplicación del germoplasma de u a de la UNALM e identificar las entradas con mayor rendimiento de grano seco y calidad del grano tostado. La evaluación morfológica se realizó usando 30 descriptores agromorfológicos del frijol común y la caracterización molecular se hizo con la técnica RAPD, empleando seis iniciadores decaméricos. Según el análisis morfológico y molecular no se encontró duplicación de germoplasma en el material evaluado, y los dendogramas no forman grupos según el origen geográfico. Las entradas con mayor rendimiento de grano seco son UNALM-18, UNALM-16 y UNALM-15 con 1458.4, 1451.6 y 1337.4 kg/ha, respectivamente; mientras que las entradas con buena calidad de granos y mayor volumen de granos reventados son UNALM-15, UNALM-19 y UNALM-18 con 10.11, 9.46 y 7.04 ml de expansión. Estas entradas tienen alta potencialidad para su explotación comercial, las que deberán priorizarse en los programas de mejoramiento genético con la finalidad de generar nuevas variedades que respondan a las exigencias de mercado. Esto puede generar mayores ingresos y mejorar las condiciones de vida de los agricultores andinos. The popping bean ( u a) is a species originated in the Andean central region, their seeds have high protein contain and are consumed toasted. A study was conducted on 24 accessions in order to determine the duplication level of u a germplasm maintained at the UNALM, and to identify accessions with high dry seed yield and toasted seed quality. The morphological evaluation was made according to 30 descriptors of common bean; and the molecular characterization was made using RAPD technique, with 6 decameric primers. Morphological and molecular analyses did not reveal germplasm duplication in the evaluated material, and dendograms did not show clustering of accessions according to geographic origin. Accessions with high seed yield were UNALM-18, UNALM-16 and UNALM-15 with 1458.4, 1451.6 and 1337.4 kg/ha respectively, whereas the accessions with good seed quality and the highest volume of burst grains are UNALM-15, UNALM-19 and UNALM-18 with expansion of 10.11, 9.46 and 7,04 ml. These accessions have high potentiality for their commercial exploitation and should be prioritized in plant breeding programs in order to create new varieties that respond to the market exigencies. This could generate greater incomes and improve the life quality of Andean
Mise au point d'une technique de culture in vitro d'embryons immatures de Phaseolus
Guy Mergeai,Véronique Schmit,Béatrice Lecomte,Jean-Pierre Baudoin
Biotechnologie, Agronomie, Société et Environnement , 1997,
Abstract: Development of an in vitro culture technique for immature Phaseolus embryos. In the interspecific crosses Phaseolus polyanthus (or P. coccineus) (i?) x P. vulgaris, the hybrid embryos abort very early. Therefore, it is essentiel to develop an in vitro culture technique that allows the rescue of beau embryos at globular or early heart-shaped stages. After several trials conceming the salts composition, the sugar rate and the amino acid concentration of différent in vitro culture media, a technique has been developed for heart-shaped Phaseolus embryos. This technique consists of two stages. In a first step, embryos are cultivated under darkness until their germination on a medium containing the salts of Gamborg et al. (1968), 400 mg . 1` (5mM - 1 -') NHNO,, 1 mg . 1-' thiamine HCI, 5 mg . l` nicotinic acid, 0.5 mg - l` pyridoxine, 1,000 mg . l` -glutamine, 1,000 mg . l` casein hydrolysate, 100 mg . l` myo-inositol, 0.028 mg . P N6-benzyladenine, 30 g . l` sucrase, and 8 g -1-' DIFCO agar. After germination, the embryos are cultivated under light on a second medium that does not contain any NHNO, complément and is poorer in amino acids (100 mg 1-' L-glutamine). Developed with six deys old heart-shaped embryos of the P. vulgaris Bico de Ouro (NI 637) variety, this technique has proved its efficiency with other P. vulgaris and P. polyanthus génotypes. It allows an average régénération rate of 30% from the total number of cultivated embryos.
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