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Search Results: 1 - 10 of 2848 matches for " Jaramillo-Arango "
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Mannheimiosis bovina: etiología, prevención y control
Jaramillo-Arango, Carlos Julio;Trigo Tavera, Francisco J.;Suárez-Güemes, Francisco;
Veterinaria México , 2009,
Abstract: mannheimia haemolytica (mh) is the most pathogenic bacteria associated with bovine pneumonic pasteurellosis (mannheimiosis); furthermore, it is the most important economic loss disease in beef cattle, and the second one after gastrointestinal diseases mainly in less than a year old dairy heifers. it is a common and important opportunistic agent in bovine nasopharynx. stress immunosuppression or the infection caused by respiratory viruses or mycoplasma spp, lead to its establishment and multiplication in lung tissue. a1 and a6 are the most frequent serotypes in pneumonic injuries, and a1 and a2 in the nasopharynx of healthy bovine. among the mh virulence factors, leukotoxin is the most important one; its primarily toxic effect is primarily against leukocytes in ruminants. a better understanding of the epidemiology and the importance of mh species requires new identification criteria that include molecular techniques, as well as more sensitive procedures regarding biochemical and immunological isolation and identification. antimicrobial efficacy, as prophylactic or therapeutical agents, has been very variable due to diagnosis inaccuracies and to the increase of multi-resistant strains. there is a varied range of bacterins that have been used over the last decades; the efficacy of many of them has been questioned as they only protect partially and some of them might even increase the morbility. vaccines with a supernatant culture containing leukotoxin and other soluble antigens, or isolated bacterial extracts or combined with bacterins, have been recently developed showing satisfactory results. efficient prevention and control of bovine mannheimiosis should be supported by a reliable diagnosis, use of vaccines and efficient therapeutic measurements, together with good management practices.
Mannheimiosis bovina: etiología, prevención y control
Carlos Julio Jaramillo-Arango,Francisco J. Trigo Tavera,Francisco Suárez-Güemes
Veterinaria México , 2009,
Abstract: Mannheimia haemolytica (Mh) es la bacteria más patógena y más comúnmente asociada con la pasteurelosis neumónica (mann-heimiosis) bovina, la enfermedad económicamente más importante en bovinos productores de carne, y la segunda, después de las enfermedades grastrointestinales, en becerras lecheras, principalmente menores de un a o. Es un habitante normal y un importante agente oportunista de la nasofaringe de bovinos; la inmunosupresión por estrés o la infección por virus respiratorios o por Mycoplasma spp, propician su establecimiento y multiplicación en el tejido pulmonar. El A1 y el A6 son los serotipos más frecuentes en lesiones neumónicas, y el A1 y A2 en nasofaringe de bovinos sanos. Entre los factores de virulencia de Mh, la leucotoxina es el más importante, cuyo efecto tóxico primario es en contra de los leucocitos, particularmente de rumiantes. Para comprender mejor la epidemiología y la importancia de las especies de Mh, se requieren nuevos criterios para su identificación, que incluyan técnicas moleculares y procedimientos más sensibles de aislamiento e identificación bioquímica e inmunológica. La eficacia de los antimicrobianos, como profilácticos o terapéuticos, ha sido muy variable debido a inconsistencias en el diagnóstico y al incremento en la frecuencia de cepas multirresitentes. Existe una amplia gama de bacterinas empleadas durante décadas; sin embargo, la eficacia de muchas de ellas ha sido cuestionada, pues sólo protegen parcialmente, incluso algunas pueden incrementar la morbilidad. Recientemente se han desarrollado vacunas con sobrenadante de cultivo que contienen leucotoxina y otros antígenos solubles, o extractos bacterianos solos o combinados con bacterinas, con resultados muy satisfactorios. La prevención y el control eficaz de la mannheimiosis bovina deben sustentarse en un diagnóstico confiable, vacunas y medidas terapéuticas eficaces, y buenas prácticas de manejo.
Resistencia a antimicrobianos en cepas de Mannheimia haemolytica aisladas de exudado nasal de bovinos productores de leche
María Luisa Samaniego B.,José Luis Contreras J.,Carlos J. Jaramillo-Arango,Francisco Aguilar-Romero
Veterinaria México , 2012,
Abstract: Se emplearon 201 cepas de Mannheimia haemolytica provenientes de muestras de exudado nasal de bovinos productores de leche, 123 de bovinos clínicamente sanos (CS) y 78 de bovinos enfermos de neumonía (CE), obtenidas de un complejo lechero en la región de Tizayuca, Hidalgo, México, las cuales fueron identificadas previamente mediante pruebas convencionales de cultivo y bioquímicas, y serotipificadas mediante la prueba de hemaglutinación indirecta. Se les realizó la prueba de difusión en placa para determinar la resistencia a diversos antimicrobianos como ampicilina, gentamicina, ceftiofiur, penicilina, estreptomicina, trimetoprim con sulfametoxazol, tetraciclina y eritromicina. Las frecuencias más altas en la resistencia a antimicrobianos se presentaron a la estreptomicina (81.6%) y gentamicina (24.4%), todas las cepas fueron susceptibles a la ampicilina y penicilina. Debido a la alta frecuencia (81.6%) de cepas de M. haemolytica resistentes a St con la técnica de Kirby-Bauer, se buscó la presencia del gen strA. Se realizó la técnica de PCR para comprobar la presencia del gen strA que codifica para la enzima aminoglycoside-3-phosphotransferase que proporciona resistencia contra la estreptomicina. Del total de cepas estudiadas (n = 201), 42.7% presentaron el gen strA, del cual 17.4% pertenecía al serotipo A1, 1.4% al A6 y 23.8% a cepas no tipificables. De las 78 cepas de CE y las 123 de CS, 80.0% y 18.7% respectivamente, presentaron el gen strA.
Characterisation of Mannheimia Sp. and P. multocida Strains Isolated from Bovine Pneumonic Lungs in Two Slaughterhouses in Mexico
C.J. Jaramillo-Arango,R. Hernandez-Castro,V.Campuzano-Ocampo,F. Suarez-Guemes,R. Delgado-Gonzalez,F. Trigo-Tavera
Journal of Animal and Veterinary Advances , 2012,
Abstract: Mannheimia sp. and P. multocida (Pm) strains were isolated from pneumonic lungs (n = 475) which were obtained in two slaughterhouses in Mexico. Isolation and phenotyping were carried out by means of in vitro culture, as well as biochemical and immunological tests. Of the 131 isolates 60.3% was Pm and 39.7% was Mannheimia sp. The isolating rate in lungs was 16.6% for Pm, 10.7% M. haemolytica (Mh) and 0.2% M. glucosida (Mg) in both slaughterhouses. These differences were significant (p 0.05). Out of 52 Mannheimia sp. isolations, 98% was Mh and 1.9% Mg. Out of 51 Mh isolates 33% was of Serotype 1(S1), 17.6% S6 and 49% Non-Typable (NT). These differences were significant (p 0.05). Out of 79 Pm isolations, 98.7% was biotype A and 1.2% biotype D. These differences were significant (p 0.05). Pm got the highest rate of isolations in lungs which differs from data of previous results where Mh is considered as the most pathogenic bacteria and the most commonly associated to bovine pasteurelosis pneumonic. The highest frequency of Mh S1 and Pm biotype A confirms that these two bacteria are the most commonly found in pneumonic lungs in Mexico.
Study of the Population Structure of Dogs in a Political District in Mexico City
.A. Romero-Lopez,C.J. Jaramillo-Arango,J.J. Martinez-Maya,E. alvarez Peralta,C. Robert Terrones
Journal of Animal and Veterinary Advances , 2012,
Abstract: A study was followed in order to measure the canine population structure according to dog ownership variables and social stratum of housing in the political district of Alvaro Obregon in Mexico City. Through a stratified random sampling, the number of housing to be sampled was determined. Total 2436 questionnaires were applied in high (397), medium (1029) and low (1010) social strata. There was a significant difference (p<0.05) in the frequency of homes with dogs, number of dogs per housing, male: female ratio, dog: human ratio, breed, preventive treatments, ownership aspects, handling habits, cause of death and type of shelter. Life expectancy was 0-2 years for the three strata in approximately 1.8 periods. The difference in the population structure among the strata may lead to preventive actions, control and eradication of dog diseases or zoonoses. It is necessary to implement programs to limit canine population growth, as dogs may be an important etiology in human diseases.
Frecuencia de aislamientos de Mannheimia haemolytica y Pasteurella multocida en becerras con signos clínicos de enfermedad respiratoria, en un complejo lechero del estado de Hidalgo, México
José Luis de la Rosa Romero,Carlos Julio Jaramillo-Arango,José Juan Martínez-Maya,Francisco Aguilar-Romero
Veterinaria México , 2012,
Abstract: Se determinó la frecuencia de Mannheimia haemolytica (Mh) y Pasteurella multocida (Pm) obtenidas de exudado nasal de becerras afectadas por enfermedad respiratoria, en un complejo lechero del estado de Hidalgo, México, evaluadas durante 5 meses en un estudio descriptivo transversal. El aislamiento e identificación se hizo mediante procedimientos selectivos y pruebas bioquímicas. Se evaluó la asociación de algunas variables con el aislamiento de Mh y Pm, mediante Ji cuadrada o Fisher, el cálculo de la razón de momios y el análisis de regresión logística. De 239 becerras menores de un a o, estudiadas, en 84 (35.14%) se aisló Mh o Pm, de ellas, 67 (28.03%) con Mh y 17 (7.11%) con Pm; en 8 becerras (3.10%) se aislaron ambos microorganismos. Se evaluaron posibles factores de riesgo: alojamiento, tratamiento y vacunación. La frecuencia de aislamientos de Mh fue mayor que la de Pm en becerras alojadas en becerreras o en corrales (P 0.05), o que estaban en becerreras a la intemperie (P 0.05), similarmente, la frecuencia de Mh y Pm juntas, fue mayor en becerras no vacunadas contra rinotraqueitis infecciosa bovina (RM = 2.93, P 0.05), diarrea viral bovina (RM = 4.26, P 0.05), parainfluenza 3PI3 (RM = 2.68, P 0.05), virus respiratorio sincitial bovino (RM = 2.36, P 0.05) y mannheimiosis (RM = 1.97, P 0.05). Las variables que permanecieron en el modelo de regresión fueron alojar las becerras en los establos y la no vacunación contra diarrea viral bovina. Mh presentó la mayor tasa de aislamientos en becerras alojadas tanto en becerreras individuales como en corrales o a la intemperie.
Prevalence of Mannheimia haemolytica Isolated from Bovine Nasal Exudates and Associated Factors, in Dairy Farms in the North-Central of Mexico
C.J. Jaramillo-Arango,R. Hernandez-Castro,F. Suarez-Guemes,J.J. Martinez-Maya,F. Aguilar-Romero,L. Jaramillo-Meza,F.J. Trigo
Journal of Animal and Veterinary Advances , 2012,
Abstract: Mannheimia sp. strains obtained from bovine nasal exudates of either clinically healthy (n = 1902) or infected with pneumonia animals (n = 189) were isolated and characterised to estimate the prevalence of isolated serotypes and to identify some factors associated to prevalence in dairy farms in Mexico, by means of a transectional descriptive study. Strains were isolated and typified through conventional in vitro culture methods, biochemical and immunological tests. Chi square or fisher statistical tests were applied, as well as odds ratio calculation and logistic regression analysis to evaluate the association and effect of some variables on Mannheimia sp. isolation. Isolates were composed in 48% by serotype A1, in 2.4% by A6 and 49.3% were non-typable. The apparent prevalence rates of Mannheimia haemolytica were significantly higher in diseased bovines (OR = 2.54; p< 0.05), as well as in bovines younger than 1 year of age (OR = 2.26; p< 0.05). Health condition and age were the variables that remained in the logistic regression model. Serotype A1 showed the highest prevalence, even when most isolates were not-typable. Bovines younger than one year of age and those with respiratory disease were the groups with the highest frequency of M. haemolytica isolates.
Teniosis y detección de anticuerpos anticisticerco en personas de una comunidad rural del estado de Guerrero
Martínez-Maya,José Juan; Aluja,Aline S de; Avila-Ramírez,Guillermina; Aguilar-Vega,Laura; Plancarte-Crespo,Agustín; Jaramillo-Arango,Carlos Julio;
Salud Pública de México , 2003, DOI: 10.1590/S0036-36342003000200003
Abstract: objective: to assess the frequency of taenia solium carriers and its relationship with human cysticercosis in a mexican locality. material and methods: a cross-sectional study was conducted in 1998, in a locality of guerrero state, mexico. four hundred and three fecal samples were analyzed by enzyme-linked immunosorbent assay (elisa) to detect taenia sp coproantigen. ninety two serum samples were also analyzed for antibodies against cisticerci using the immunoelectrotransfer blot assay (iet). data were analyzed using descriptive statistics and odds ratios. results: five of the 403 fecal samples were positive (1.2%). the adult cestode was recovered in only two people. three (3.26%) out of the 92 serum samples that were analyzed for anticysticercus antibodies were positive. seventeen serum samples corresponded to people living with a person positive to the coproantigen test (first group), the remaining 75 were obtained from people without a history of releasing taenia proglottids (second group). in the first group, 2 positive sera were detected (11.8%), while in the second only 1 was positive (1.3%) (odds ratio= 9.87, 95% confidence interval 0.64 to 295.56, p= 0.08). conclusions: the difficulty to obtain the adult parasite in persons positive to coproantigens, may be due to difficult expulsion, a shorter permanence of the parasite in the host, insufficient dosage of treatment, or to lack of specificity of the diagnostic test. further studies are needed to evaluate these possibilities; a better knowledge of parasite transmission dynamics will allow the implementation of prevention and control measures and a better assessment of diagnostic tests under field conditions.
The UNC-method: a problem-based software development method
Zapata Jaramillo,Carlos Mario; Arango Isaza,Fernando;
Ingeniería e Investigación , 2009,
Abstract: software engineers use development methods to guarantee on-time delivery, keeping to budget and quality in their software applications. there are two kinds of development methods: plan-driven and agile methods. both of them still have problems; these refer to resolving problems instead of thinking about them, they use informal or semi-formal artefacts and they leave consistency management to the analysts. the unc-method (a problem-based software development method) is defined in this paper. the unc-method is currently being developed in the universidad nacional de colombia; it has been used by students from the school of systems as part of their training in methodological software development during the last five years. the unc-method is a mixture of well-known artefacts (i.e. uml diagrams and graphical user interfaces) and non-traditional approaches (e.g. cause-and-effect diagram, kaos goal diagrams and pre-conceptual schemas) used in trying to overcome the aforementioned problems. a case study is also used for exemplification purposes.
UNC-Method: un método de desarrollo de software basado en problemas The UNC-method: a problem-based software development method
Zapata Jaramillo Carlos Mario,Arango Isaza Fernando
Ingeniería e Investigación , 2009,
Abstract: Los ingenieros de software emplean los métodos de desarrollo para garantizar la entrega puntual, el cumplimiento de los presu-puestos y la calidad de las aplicaciones de software. Existen dos tipos de métodos de desarrollo: los dirigidos por planes, y los á-giles. Ambos, aún presentan problemas: se refieren a la solución en lugar de pensar en el problema, usan artefactos formales o semiformales y dejan el manejo de la consistencia en manos de los analistas. En este artículo se define UNC-Method, un méto-do de desarrollo de software basado en problemas, que se viene desarrollando en la Universidad Nacional de Colombia y que se usa en la Escuela de Sistemas como parte del entrenamiento en desarrollo metodológico de software a los estudiantes de di-cha universidad durante los últimos cinco a os. UNC-Method combina artefactos tradicionales del desarrollo de software (como los diagramas de UML y las interfaces gráficas de usuario) con enfoques no tradicionales en dicha disciplina (como los diagra-mas causa-efecto, los diagramas de objetivos de KAOS y los esquemas preconceptuales) en un esfuerzo por resolver los proble-mas antes mencionados. Además, se ejemplifica el método con un caso de estudio. Software engineers use development methods to guarantee on-time delivery, keeping to budget and quality in their software applications. There are two kinds of development methods: plan-driven and agile methods. Both of them still have problems; these refer to resolving problems instead of thinking about them, they use informal or semi-formal artefacts and they leave consis-tency management to the analysts. The UNC-method (a problem-based software development method) is defined in this paper. The UNC-method is currently being developed in the Universidad Nacional de Colombia; it has been used by students from the School of Systems as part of their training in methodological software development during the last five years. The UNC-method is a mixture of well-known artefacts (i.e. UML diagrams and graphical user interfaces) and non-traditional approaches (e.g. cause-and-effect diagram, KAOS goal diagrams and pre-conceptual schemas) used in trying to overcome the aforementioned pro-blems. A case study is also used for exemplification purposes.
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