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Search Results: 1 - 10 of 326 matches for " Janse Ingmar "
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Development and Comparison of Two Assay Formats for Parallel Detection of Four Biothreat Pathogens by Using Suspension Microarrays
Ingmar Janse, Jasper M. Bok, Raditijo A. Hamidjaja, Hennie M. Hodemaekers, Bart J. van Rotterdam
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0031958
Abstract: Microarrays provide a powerful analytical tool for the simultaneous detection of multiple pathogens. We developed diagnostic suspension microarrays for sensitive and specific detection of the biothreat pathogens Bacillus anthracis, Yersinia pestis, Francisella tularensis and Coxiella burnetii. Two assay chemistries for amplification and labeling were developed, one method using direct hybridization and the other using target-specific primer extension, combined with hybridization to universal arrays. Asymmetric PCR products for both assay chemistries were produced by using a multiplex asymmetric PCR amplifying 16 DNA signatures (16-plex). The performances of both assay chemistries were compared and their advantages and disadvantages are discussed. The developed microarrays detected multiple signature sequences and an internal control which made it possible to confidently identify the targeted pathogens and assess their virulence potential. The microarrays were highly specific and detected various strains of the targeted pathogens. Detection limits for the different pathogen signatures were similar or slightly higher compared to real-time PCR. Probit analysis showed that even a few genomic copies could be detected with 95% confidence. The microarrays detected DNA from different pathogens mixed in different ratios and from spiked or naturally contaminated samples. The assays that were developed have a potential for application in surveillance and diagnostics.
Reliable detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis by using multiplex qPCR including internal controls for nucleic acid extraction and amplification
Ingmar Janse, Raditijo A Hamidjaja, Jasper M Bok, Bart J van Rotterdam
BMC Microbiology , 2010, DOI: 10.1186/1471-2180-10-314
Abstract: Multiplex real-time PCRs were designed for rapid and reliable detection of three major pathogens that have the potential to cause high morbidity and mortality in humans: B. anthracis, F. tularensis and Y. pestis. The developed assays detect three pathogen-specific targets, including at least one chromosomal target, and one target from B. thuringiensis which is used as an internal control for nucleic acid extraction from refractory spores as well as successful DNA amplification. Validation of the PCRs showed a high analytical sensitivity, specificity and coverage of diverse pathogen strains.The multiplex qPCR assays that were developed allow the rapid detection of 3 pathogen-specific targets simultaneously, without compromising sensitivity. The application of B. thuringiensis spores as internal controls further reduces false negative results. This ensures highly reliable detection, while template consumption and laboratory effort are kept at a minimumA group of diverse pathogens has the potential to cause high morbidity and mortality in humans -especially if carried by aerosols- even though they do not pose a major threat to public health under normal circumstances. The most menacing bacterial pathogens of this group are Bacillus anthracis, Francisella tularensis and Yersinia pestis, and these organisms are listed as category A biothreat agents (classification of the CDC, USA, http://www.bt.cdc.gov/agent/agentlist-category.asp webcite) because of the potential danger of their deliberate release. Exposure to aerosolized B. anthracis spores and F. tularensis can lead to inhalational anthrax and tularemia. Y. pestis may cause pneumonic plague, which, unlike the other two diseases, may also spread from person to person.To reduce the public health impact of such highly pathogenic micro-organisms, rapid and accurate diagnostic tools for their detection are needed. Timely recognition of disease agents will enable appropriate treatment of exposed individuals which will be cr
Multiplex qPCR for reliable detection and differentiation of Burkholderia mallei and Burkholderia pseudomallei
Janse Ingmar,Hamidjaja Raditijo A,Hendriks Amber CA,van Rotterdam Bart J
BMC Infectious Diseases , 2013, DOI: 10.1186/1471-2334-13-86
Abstract: Background Burkholderia mallei and B. pseudomallei are two closely related species of highly virulent bacteria that can be difficult to detect. Pathogenic Burkholderia are endemic in many regions worldwide and cases of infection, sometimes brought by travelers from unsuspected regions, also occur elsewhere. Rapid, sensitive methods for identification of B. mallei and B. pseudomallei are urgently needed in the interests of patient treatment and epidemiological surveillance. Methods Signature sequences for sensitive, specific detection of pathogenic Burkholderia based on published genomes were identified and a qPCR assay was designed and validated. Results A single-reaction quadruplex qPCR assay for the detection of pathogenic Burkholderia, which includes a marker for internal control of DNA extraction and amplification, was developed. The assay permits differentiation of B. mallei and B. pseudomallei strains, and probit analysis showed a very low detection limit. Use of a multicopy signature sequence permits detection of less than 1 genome equivalent per reaction. Conclusions The new assay permits rapid detection of pathogenic Burkholderia and combines enhanced sensitivity, species differentiation, and inclusion of an internal control for both DNA extraction and PCR amplification.
Molecular typing of Coxiella burnetii from animal and environmental matrices during Q fever epidemics in the Netherlands
Arnout de Bruin, Pleunie TW van Alphen, Rozemarijn QJ van der Plaats, Lianne ND de Heer, Chantal BEM Reusken, Bart J van Rotterdam, Ingmar Janse
BMC Veterinary Research , 2012, DOI: 10.1186/1746-6148-8-165
Abstract: We used a multiplex multi-locus variable number of tandem repeats analysis (MLVA) assay to investigate the genotypic diversity of C. burnetii in different types of samples that were collected nationwide during the Dutch Q fever outbreaks between 2007 and 2010. Typing was performed on C. burnetii positive samples obtained from several independent studies investigating C. burnetii presence in animals and the environment. Six different genotypes were identified on 45 farm locations, based on sequence-confirmed estimates of repeat numbers of six MLVA markers. MLVA genotype A was observed on 38 of the 45 selected farm locations in animals and in environmental samples.Sequence confirmation of the numbers of tandem repeats within each locus and consensus about repeat identification is essential for accurate MLVA typing of C. burnetii. MLVA genotype A is the most common genotype in animal samples obtained from goat, sheep, and rats, as well as in environmental samples such as (aerosolized) dust, which is considered to be the major transmission route from animals via the environment to humans. The finding of a single dominant MLVA genotype in patients, the environment, and livestock complicates accurate source-finding. Pinpointing individual sources in the Netherlands requires discrimination of genotypes at a higher resolution than attained by using MLVA, as it is likely that the dominant C. burnetii MLVA type will be detected on several farms and in different patients in a particular area of interest.Q fever, caused by the bacterium Coxiella burnetii, has been a public health problem in the Netherlands between 2007 and 2010 [1-4]. Between 2005 and 2007, before the first documented Q fever outbreak in the Netherlands, C. burnetii related abortions were reported on a number of commercial dairy goat farms in a rural area in the southeast of the Netherlands [1]. In 2007, a Q fever outbreak was reported in humans in the same rural area, which expanded to other areas in the Nethe
E.M. Uhlenbeck (1913-2003) and the Royal Netherlands Institute of Southeast Asian and Caribbean Studies (KITLV)
Mark Janse
Bijdragen tot de Taal-, Land- en Volkenkunde , 2008,
Abstract: Eugenius Marius Uhlenbeck (1913-2003) needs no introduction to readers of this journal. Bob, as many knew him, was editor of the Bijdragen tot de Taal-, Land- en Volkenkunde from 1949 until 1958. He took up this task after he obtained his PhD for his dissertation on the structure of the Javanese morpheme, and remained as editor until he was appointed chair in general linguistics at Leiden University, where he also held the chair in Javanese language and literature from 1950 until 1983.2 During his lifetime, he contributed fourteen articles to the journal. Many of these have become classics in their fields, particularly his studies of various aspects of Modern Javanese morphology and his interpretation of several Old Javanese texts. In addition, he also published eleven reviews in the Bijdragen. Among these, his article on Zoetmulder’s study of language of the Adiparwa (1950) deserves special mention. In addition to his contributions to the Bijdragen, the Royal Netherlands Institute of Southeast Asian and Caribbean Studies (KITLV) published five important monographs written by him. This brings me to the focus of this paper: the relationship between Uhlenbeck and the KITLV.
The works of E.M. Uhlenbeck (1913-2003): An annotated bibliography
Mark Janse
Bijdragen tot de Taal-, Land- en Volkenkunde , 2008,
Abstract: Eugenius Marius Uhlenbeck – Bob as he was known to his friends – was a multifacited (or many-sided) and multi-talented person. He was an accomplished scholar in the fields of Javanese language and literature and in general linguistics holding the hairs in these disciplines at Leiden University from 1950-1983 and 1958-1979 respectively. In the Netherlands and abroad he was widely acclaimed as an elder ‘statesman’. His publications reflect his rare combination of talents. I will limit myself here to brief comments about his publications on linguistics, both general and Javanese linguistics.
Fighting terrorism in the Netherlands; a historical perspective
Ronald Janse
Utrecht Law Review , 2005,
Abstract: The Dutch government and legislature are in the process of introducing an unprecedented set of anti-terrorist measures. It is claimed that these measures are necessary and justified, as terrorism today threatens the security of Dutch society as never before. But does it? In the present state of excitement, it is all too easy to forget that Dutch society has had to face terrorism before. Indeed, if the number of people killed or targeted is a measure of the gravity of a terrorist threat, terrorism in the 1970s was more serious than current terrorism has been up until now. Yet in the 1970s, the Dutch government did not introduce a comprehensive set of anti-terrorist measures, unlike, for example, the British and German governments. In this paper, the Moluccan actions, the most serious terrorist actions Dutch society has experienced so far, and the reaction by the government to these actions, are discussed. This leads to the conclusion that current terrorism is less different from old-style terrorism than the government claims it to be, although there may be one important difference: the risk that current terrorists use deeply destructive weapons. This is not to say that the government’s policies were better in the 1970s than they are now. However, a sense of what the record tells us may help us in disciplining current fears and in taking a more critical stance towards the view that the present anti-terrorist measures are necessary and justified because we never saw anything like current terrorism before.
Rijk Timmer, Profeet in eigen land. Philips van Leiden en het publiek belang
Antheun Janse
BMGN : Low Countries Historical Review , 2010,
E.A. Hattinga van 't Sant, Convivium, aangeboden aan prof. jkvr. dr. J. M. van Winter bij haar afscheid als hoogleraar aan de Rijksuniversiteit Utrecht
Over stadsgeschiedenis, voor Johanna Maria van Winter, Utrechtse Historische Cahiers, IX

A. Janse
BMGN : Low Countries Historical Review , 1990,
Ridderslag en ridderlijkheid in laat-middeleeuws Holland
A. Janse
BMGN : Low Countries Historical Review , 1997,
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