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The Uthokawiphatprasit Watergate: A Man-Made Change in Pak Phanang River Basin
Walailak Journal of Science and Technology , 2006, DOI: 10.2004/vol3iss2pp131-143
Abstract: This minireview article investigates the environmental changes since the operation of the Uthokawiphatprasit watergate over the Pak Phanang River in the southern east coast of Thailand. It was revealed that the watergate caused the slow down of water circulation in the bay which accelerated the rate of sediment deposition. The watergate also resulted in poor water quality in the Pak Phanang River which caused the declination of fish species and fish production. Moreover, it resulted in a lower production of Nipa Palm which is an important economic tree in the basin. Finally, there was evidence that the watergate might cause the spread of Malaria in this area.
Growth Performance, Carcass Quality, Visceral Organs and Intestinal Histology in Broilers Fed Dietary Dried Fermented Ginger and/or Fermented Corncob Powder  [PDF]
Duddoa Khonyoung, Janjira Sittiya, Koh-en Yamauchi
Food and Nutrition Sciences (FNS) , 2017, DOI: 10.4236/fns.2017.85039
Abstract: To determine whether dried fermented ginger (DFG), fermented with Japanese mugwort silage juice, could be replaced by fermented corncob powder (FCP) as a of feed ingredient source without significant body weight decrease or damage to visceral organs (using gross anatomical observation), to intestinal villi (using light microscopy), or to the epithelial cells on the villus apical surface (using scanning electron microscopy) the following investigation was performed. Sixty-four male broilers were allotted to 4 groups: a basal diet group (control group), and basal diet groups with DFG at a level of 50 ppm; with DFG at 50 ppm and FCP at 250 ppm (50 ppm DFG + 250 ppm FCP group); and with FCP at a level of 500 ppm (500 ppm FCP group). Feed intake, body weight gain, feed efficiency, carcass quality, small intestinal length and weight, and visceral organ weight were not different among groups. Furthermore, regarding intestinal villus height, villus area and crypt depth, a significant difference was not found among the groups. When these values of the control were expressed as an index of 100, the duodenal villus height of the 50 ppm DFG + 250 ppm FCP group and the 500 ppm FCP group were 114 and 119, respectively. The duodenal villus area of the 50 ppm DFG + 250 ppm FCP group and the 500 ppm FCP group were 125 and 158, respectively. These villus heights and areas are thought to be activated. On the epithelial cells on the villus apical surface in the duodenum and jejunum, the 50 ppm DFG + 250 ppm FCP group had protuberated cells into the intestinal lumen and deeper cells at the sites of recently exfoliated cells, suggesting that these cells are activated. The present results indicate that small amounts of fermented corncob powder can be used as a feed supplement when mixed with fermented ginger powder, due to the synergy between the two ingredients, resulting in a 6% increase in body weight gain。
Janjira Panchompoo,Richard G. Compton
电化学 , 2012,
Abstract: 荧光胺是一种非荧光剂,其易与伯胺反应形成荧光产物,被普遍用于伯胺的荧光光谱定量分析.本文利用荧光胺与伯胺反应发展了一种新型灵敏的伏安法用于检测水溶液中的伯胺.首先,在有、无伯胺的0.1molL-1PBS(pH9.0)缓冲液中,研究了玻碳电极表面荧光胺的循环伏安电化学行为.荧光胺的不可逆氧化峰出现在0.70V(vs.SCE),当加入伯胺时,在0.46V(vs.SCE)出现另一不可逆的氧化峰,为荧光胺与伯胺反应的产物.继续加入氨水,荧光胺的氧化峰变弱,反应产物的氧化峰则由于荧光胺按反应化学计量比随氨消耗增多而随之增大.上述两个阳极峰分别对应于荧光胺及其反应产物,采用方波伏安和荧光光谱技术可实现水溶液中伯胺的定量检测.在0~60μmolL-1氨浓度范围内,该反应产物方波伏安检测成线性响应.S/N=3或3σ时检测下限分别为0.71μmolL-1和3.17μmolL-1,与荧光法检测的结果相近.
Genomic and expression analysis of glycosyl hydrolase family 35 genes from rice (Oryza sativa L.)
Waraporn Tanthanuch, Mallika Chantarangsee, Janjira Maneesan, James Ketudat-Cairns
BMC Plant Biology , 2008, DOI: 10.1186/1471-2229-8-84
Abstract: Fifteen rice Bgal genes were identified in the plant genome, one of which encodes a protein similar to animal Bgals (OsBgal9), and the remaining 14 fall in a nearly plant-specific subfamily of Bgals. The presence of both classes of Bgals in bryophytes, as well as vascular plants, suggests both gene lineages were present early in plant evolution. All 15 proteins were predicted to contain secretory signal sequences, suggesting they have secretory pathway or external roles. RT-PCR and database analysis found two distinct lineages to be expressed nearly exclusively in reproductive tissues and to be closely related to Arabidopsis Bgals expressed most highly in flower and pollen. On the other hand, OsBgal6 is expressed primarily in young vegetative tissues, and alternative splicing in panicle prevents its production of full-length protein in this reproductive tissue. OsBgal11 also showed alternative splicing to produce different length proteins. OsBgal13 produced by recombinant expression in Escherichia coli hydrolyzed α-L-arabinoside in addition to β-D-galactoside and β-(1→3)-, β-(1→4)- and β-(1→6)- linked galacto-oligosaccharides.Rice GH35 contains fifteen genes with a diversity of protein sequences, predicted locations and expression and splicing patterns that suggest that OsBgals enzymes may play a variety of roles in metabolism of cell wall polysaccharides, glycoproteins and glycolipids.β-Galactosidases (Bgals) (EC catalyze the hydrolysis of the glycosidic bonds of terminal non-reducing β-D-galactosyl residues of oligosaccharides and β-D-galactopyranosides. This group of enzymes has a broad distribution, which encompasses plants, animals and microorganisms. The biological functions of these enzymes include degradation of structural polysaccharides in plant cell walls [1,2], thereby acting to control fruit softening during ripening [3], hydrolysis of dietary lactose [4,5] and degradation of glycolipids and proteoglycans in mammals [6,7], and metabolism of la
Identification of Infection Pathways and Development of Inoculation Methods for Peanut Using Green-Fluorescence-Protein Aspergillus Flavus
Janjira Puntase,Chuckree Senthong,Keith T. Ingram,Arthur K. Weissinger
Agricultural Journal , 2013,
Abstract: Screening peanut (Arachis hypogea L.) germplasm for resistance to Aspergillus flavus was hampered by low and variable rates of infection. Three experiments were conducted to develop methods to inoculate Aspergillus flavus on peanut flowers, pegs and ovaries by strains of A. flavus, modified to produce a Green Fluorescence Protein (GFP). Maximum infection with GFP A. flavus was found by spraying an aqueous suspension of conidia over shoots and flowers of peanut which resulted in 100% infection of floral surfaces. Other methods tested included application of cracked corn inoculum to the surface of soil in cuvettes and mixing aqueous suspension or cracked corn inoculum with soil in cuvettes, designed to allow in situ observation of peg and pod development. Observations with an ultra violet-illuminated microscope showed fluorescence of GFP A. flavus on the surface of the peanut flowers and a fluorescing network of hyphae on ovules inside the peanut pegs before the pegs reached the soil surface. These experiments provide supporting evidence that A. flavus infection of peanut ovaries can occur during flowering or early peg formation.
Assessment of gastrointestinal stromal tumors with computed tomography following treatment with imatinib mesylate
Sith Phongkitkarun, Cholada Phaisanphrukkun, Janjira Jatchavala, Ekaphop Sirachainan
World Journal of Gastroenterology , 2008,
Abstract: AIM: To evaluate and characterize the patterns of disease progression of metastatic or unresectable gastrointestinal stromal tumor (GIST) treated with imatinib mesylate, and to determine the prognostic significance associated with disease progression.METHODS: Clinical data and computed tomography (CT) images were retrospectively reviewed in 17 GIST patients who were treated with imatinib mesylate from October 2002 to October 2006. Apart from using size measurement for evaluation of tumor response [Response Evaluation Criteria in Solid Tumors (RECIST) criteria], patterns of CT changes during treatment were evaluated and correlated with clinical data.RESULTS: There were eight non-responders and nine responders. Five patterns of CT change during treatment were found: focal progression (FP), generalized progression (GP), generalized cystic change (GC), new cystic lesion (NC) and new solid lesion (NS). At the end of study, all non-responders showed GP, whereas responders showed cystic change (GC and NC) and response according to RECIST criteria. Overall survival was significantly better in patients with cystic change or response within the RECIST criteria compared with GP patients (P = 0.0271).CONCLUSION: Various patterns of CT change in patients with GIST who responded to imatinib mesylate were demonstrated, and might determine the prognosis of the disease. A combination of RECIST criteria and pattern of CT change are proposed for response evaluation in GIST.
Sensorineural hearing loss after concurrent chemoradiotherapy in nasopharyngeal cancer patients
Janjira Petsuksiri, Achariyaporn Sermsree, Kullathorn Thephamongkhol, Phawin Keskool, Kanthong Thongyai, Yaowalak Chansilpa, Pittayapoom Pattaranutaporn
Radiation Oncology , 2011, DOI: 10.1186/1748-717x-6-19
Abstract: A retrospective cohort study of 134 individual ears from 68 NPC patients, treated with conventional RT and IMRT in combination with chemotherapy from 2004-2008 was performed. Dosimetric data of the cochlea were analyzed. Significant SNHL was defined as > 15 dB increase in bone conduction threshold at 4 kHz and PTA (pure tone average of 0.5, 1, 2 kHz). Relative risk (RR) was used to determine the associated factors with the hearing threshold changes at 4 kHz and PTA.Median audiological follow up time was 14 months. The incidence of high frequency (4 kHz) SNHL was 44% for the whole group (48.75% in the conventional RT, 37% with IMRT). Internal auditory canal mean dose of > 50 Gy had shown a trend to increase the risk of high frequency SNHL (RR 2.02 with 95% CI 1.01-4.03, p = 0.047).IMRT and radiation dose limitation to the inner ear appeared to decrease SNHL.Radiation therapy (RT) is the standard treatment for nasopharyngeal cancer (NPC) patients as a result of the relative radiosensitivity, deep location and the close proximity to the normal critical structures. High dose RT of ≥ 66 Gy in combination with chemotherapy has yielded a 5-year locoregional control for more than 80% of the patients with locally advanced disease [1-3]. Consequently, RT produces undesirable side effects on the adjacent organs. In addition to xerostomia, sensorineural hearing loss (SNHL), resulting from the cochlea damage, is one of the major long term side effects which impacts the patients' quality of life. With modern conformal radiation techniques, the incidence of radiation induced SNHL is expected to decline, due to a better visualization of the organs on the planning CT images and a better capability to spare the cochlea with a mean dose < 40-50 Gy [4-7].This retrospective analytic study aims to report the incidences of SNHL of NPC patients receiving chemoradiotherapy with conventional RT comparing with intensity modulated radiation therapy (IMRT). To our knowledge, this study is the f
Molecular detection and speciation of pathogenic Leptospira spp. in blood from patients with culture-negative leptospirosis
Siriphan Boonsilp, Janjira Thaipadungpanit, Premjit Amornchai, Vanaporn Wuthiekanun, Wirongrong Chierakul, Direk Limmathurotsakul, Nicholas P Day, Sharon J Peacock
BMC Infectious Diseases , 2011, DOI: 10.1186/1471-2334-11-338
Abstract: We evaluated our hypothesis during a prospective study of 418 consecutive patients presenting to a hospital in northeast Thailand with an acute febrile illness. Admission blood samples were taken for Leptospira culture and PCR. A single tube nested PCR that amplified a region of the rrs gene was developed and applied, amplicons sequenced and a phylogenetic tree reconstructed.39/418 (9%) patients were culture-positive for Leptospira spp., and 81/418 (19%) patients were culture-negative but rrs PCR-positive. The species associated with culture-positive leptospirosis (37 L. interrogans and 2 L. borgpetersenii) were comparable to those associated with culture-negative, PCR-positive leptospirosis (76 L. interrogans, 4 L. borgpetersenii, 1 unidentified, possibly new species).Molecular speciation failed to identify a unique bacterial subset in patients with culture-negative, PCR-positive leptospirosis. The rate of false-negative culture was high, and we speculate that antibiotic pre-treatment is the most likely explanation for this.Leptospirosis is an acute febrile illness caused by pathogenic species belonging to the genus Leptospira [1,2]. This zoonotic disease has a worldwide distribution but is most common in tropical and subtropical regions and has the greatest impact on public health in developing countries [1-4]. Disease is maintained by chronic carrier hosts that excrete the organism into the environment, and infection in man results from direct contact with infected animals or indirect contact with a contaminated environment [1-3].Leptospira are present in the blood during the first week of infective symptoms [1,2]. Culture is rarely performed in routine clinical practice since this may take several months and requires considerable expertise, which places it within the domain of specialist reference centres. Culture continues to have an important role, however, in defining the global epidemiology of infection [4]. Identification of the serovar of infecting isolate
Diagnostic Accuracy of Real-Time PCR Assays Targeting 16S rRNA and lipl32 Genes for Human Leptospirosis in Thailand: A Case-Control Study
Janjira Thaipadunpanit,Wirongrong Chierakul,Vanaporn Wuthiekanun,Direk Limmathurotsakul,Premjit Amornchai,Siriphan Boonslip,Lee D. Smythe,Roongrueng Limpaiboon,Alex R. Hoffmaster,Nicholas P. J. Day,Sharon J. Peacock
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0016236
Abstract: Rapid PCR-based tests for the diagnosis of leptospirosis can provide information that contributes towards early patient management, but these have not been adopted in Thailand. Here, we compare the diagnostic sensitivity and specificity of two real-time PCR assays targeting rrs or lipL32 for the diagnosis of leptospirosis in northeast Thailand.
Emergence of Community-Associated Methicillin-Resistant Staphylococcus aureus Associated with Pediatric Infection in Cambodia
Kheng Chheng, Sarah Tarquinio, Vanaporn Wuthiekanun, Lina Sin, Janjira Thaipadungpanit, Premjit Amornchai, Ngoun Chanpheaktra, Sarinna Tumapa, Hor Putchhat, Nicholas P. J. Day, Sharon J. Peacock
PLOS ONE , 2009, DOI: 10.1371/journal.pone.0006630
Abstract: Background The incidence of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infection is rising in the developed world but appears to be rare in developing countries. One explanation for this difference is that resource poor countries lack the diagnostic microbiology facilities necessary to detect the presence of CA-MRSA carriage and infection. Methodology and Principal Findings We developed diagnostic microbiology capabilities at the Angkor Hospital for Children, Siem Reap, western Cambodia in January 2006 and in the same month identified a child with severe community-acquired impetigo caused by CA-MRSA. A study was undertaken to identify and describe additional cases presenting between January 2006 and December 2007. Bacterial isolates underwent molecular characterization using multilocus sequence typing, staphylococcal cassette chromosome mec (SCCmec) typing, and PCR for the presence of the genes encoding Panton-Valentine Leukocidin (PVL). Seventeen children were identified with CA-MRSA infection, of which 11 had skin and soft tissue infection and 6 had invasive disease. The majority of cases were unrelated in time or place. Molecular characterization identified two independent MRSA clones; fifteen isolates were sequence type (ST) 834, SCCmec type IV, PVL gene-negative, and two isolates were ST 121, SCCmec type V, PVL gene-positive. Conclusions This represents the first ever report of MRSA in Cambodia, spread of which would pose a significant threat to public health. The finding that cases were mostly unrelated in time or place suggests that these were sporadic infections in persons who were CA-MRSA carriers or contacts of carriers, rather than arising in the context of an outbreak.
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