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Search Results: 1 - 10 of 470798 matches for " Janice A. Rafael "
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Hyphal Morphology and Elongation Alterations in Aspergillus nidulans Provoked by the Diterpene Kaurenoic Acid  [PDF]
Janice A. Rafael, Nilton S. Arakawa, Sérgio R. Ambrosio, Fernando B. Da Costa, Suraia Said
Advances in Microbiology (AiM) , 2013, DOI: 10.4236/aim.2013.35059

Kaurenoic acid (KA), a kaurane-type diterpene extracted from leaves of Mikania hirsutissima, was previously reported as an inhibitor of vascular contractility mainly by blocking extracellular Ca2+ influx. The compound is known for several other biological activities such as antiparasitic, antispasmodic and antibacterial activity. The aim of the present study was to investigate the effect of KA on Aspergillus nidulans. KA (0.3 mM) showed fungistatic activity against A. nidulans with visible hyphal elongation and morphology damage. These effects were reverted by CaCl2 addition showing that KA interferes with intracellular Ca2+ gradient in A. nidulans. This is the first report on the mechanism of action of KA involving calcium levels by altering the elongation of fungi hyphae.

The Difference in Calcium Levels in Aspergillus nidulans Grown on Glucose or Pectin  [PDF]
Janice Aparecida Rafael, Suraia Said
Advances in Microbiology (AiM) , 2012, DOI: 10.4236/aim.2012.22016
Abstract: Understanding the growth regulatory mechanisms in filamentous fungi is very important for the production of medicines for antifungal therapies. It is well established that Ca2+ gradient is essential for hyphal growth and that one mechanism responsible for the Ca2+ cellular concentration starts with the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) by receptor-regulated forms of phosphoinositide-specific phospholipase C (PI-PLC). In the present study the levels of calcium in Aspergillus nidulans wild type (A26) and plcA-deficient mutant (AP27) growing in a carbon source readily assimilated, as glucose or pectin a non-readily assimilated carbon source was investigated. Intracellular calcium levels in A26 were higher in the presence of glucose than in pectin, but lower in AP27 independently of the carbon source and in AP27 the vesicular calcium distribution occurred mainly at the apex of the hyphae. Delay in nuclear division was also observed if A26 and AP27 were grown in pectin presence when compared with growth in glucose. For the first time, it is demonstrated that the levels of intracellular Ca2+ were higher when A. nidulans was growing in glucose than in a non readily assimilated carbon source as pectin. Further, it also showed that the plcA gene, although not essential, may be responsible for high-molecular weight carbon source recongnation, for the intracellular Ca2+ levels maintenance and consequently by the nuclear division in A. nidulans.
Method validation and stability study of quercetin in topical emulsions
Casagrande, Rúbia;Baracat, Marcela M.;Georgetti, Sandra R.;Verri Jr, Waldiceu A.;Vicentini, Fabiana T. M. C.;Rafael, Janice A.;Jabor, José R.;Fonseca, Maria J. V.;
Química Nova , 2009, DOI: 10.1590/S0100-40422009000700041
Abstract: this study validated a high performance liquid chromatography (hplc) method for the quantitative evaluation of quercetin in topical emulsions. the method was linear within 0.05 - 200 μg/ml range with a correlation coefficient of 0.9997, and without interference in the quercetin peak. the detection and quantitation limits were 18 and 29 ng/ml, respectively. the intra- and inter-assay precisions presented r.s.d. values lower than 2%. an average of 93% and 94% of quercetin was recovered for non-ionic and anionic emulsions, respectively. the raw material and anionic emulsion, but not non-ionic emulsion, were stable in all storage conditions for one year. the method reported is a fast and reliable hplc technique useful for quercetin determination in topical emulsions.
The Elephant in the Room: Understanding Barriers to Students’ Articulation of Diversity  [PDF]
Janice Gasker, Heather A. Campbell LaBarre
Creative Education (CE) , 2010, DOI: 10.4236/ce.2010.12011
Abstract: A scholarship competition designed to foster classroom discussion and introspection about diversity had few partici-pants despite its cash prize. This paper explores undergraduates’ view of the project via focus groups and reveals sur-prising depth in students’ answers to the question of why students did not participate. Analysis uncovered emergent themes related to emotional discomfort with diversity in general and self reflection in particular. Implications include the conceptualization of “diversity,” teaching methods, and instructors’ professional development.
A Theoretical Study of the Hidden Wounds of War: Disenfranchised Grief and the Impact on Nursing Practice
Janice A. Aloi
ISRN Nursing , 2011, DOI: 10.5402/2011/954081
Telehealth Regulatory and Legal Considerations: Frequently Asked Questions
Jana Cason,Janice A. Brannon
International Journal of Telerehabilitation , 2011, DOI: 10.5195/ijt.2011.6077
Abstract: As telehealth gains momentum as a service delivery model in the United States within the rehabilitation professions, regulatory and legal questions arise. This article examines the following questions: 1. Is there a need to secure licenses in two states (i.e., where the practitioner resides, and where the client is located), before engaging in telehealth? 2. Do state laws differ concerning if and how telehealth can occur? 3. Do any states expressly disallow telehealth? 4. Can services delivered through telehealth be billed the same way as services provided in-person? 5. If practitioners fulfill the requirements to maintain licensure (e.g., continuing education obligations) in their state of residence, do they also need to fulfill the requirements to maintain licensure for the state in which the client resides? 6. Will professional malpractice insurance cover services delivered through telehealth? 7. Does a sole practitioner need to abide by HIPAA regulations? Responses to these questions are offered to raise awareness of the regulatory and legal implications associated with the use of a telehealth service delivery model
Telomere Maintenance Mechanisms: Prognostic and Therapeutic Implications for the Pathologist and Oncologist  [PDF]
Noelyn A. Hung, Howard Hsia, Janice A. Royds, Tania L. Slatter
Open Journal of Pathology (OJPathology) , 2013, DOI: 10.4236/ojpathology.2013.31003

In neoplasia, telomere maintenance mechanisms (TMMs) can be prognostic and may direct therapy in the future. Two types of TMM, telomerase and recombination-based alternative lengthening of telomeres (ALT), result in four prognostic tumor groups when they occur individually, in combination, or in mutual absence. Correct designation of the TMM therefore requires an assessment of telomerase activity and for ALT telomere length distribution and ALT associated promyelocytic leukaemia protein (PML) bodies (APBs). The four groups are associated with differing prognoses that are dependent on the tumor type. As TMM inhibitors are developed, oncologists will require that pathologists determine the TMM, and the treatments will differ accordingly. Furthermore, any anti-TMM therapy administered has the potential to selectively change the TMM used by a tumor, necessitating reassessment of the therapeutic strategy. Herein, we review the telomere maintenance mechanisms, the current diagnostic measures and their utility as prognostic markers in the clinical setting.

Perinatal transmission of dengue virus in Puerto Rico: a case report  [PDF]
Janice Pérez-Padilla, Rafael Rosario-Casablanca, Luis Pérez-Cruz, Carmen Rivera-Dipini, Kay Marie Tomashek
Open Journal of Obstetrics and Gynecology (OJOG) , 2011, DOI: 10.4236/ojog.2011.13016
Abstract: We report a laboratory confirmed case of vertical transmission of dengue in a mother-child pair in the eastern part of Puerto Rico. The clinical course of the pregnant female suggested a GBS infection, but laboratory tests confirmed it was dengue infection, one week after delivery. The male infant was healthy at birth, but one week after birth developed clinical complications related to vertical transmission of dengue. This report targets physicians in dengue endemic countries like Puerto Rico to be aware of the possibility of vertical transmission of dengue in symptomatic pregnant patients, especially around the time of delivery.
Drosophila Tel2 Is Expressed as a Translational Fusion with EpsinR and Is a Regulator of Wingless Signaling
Ji-Hoon Lee, Janice A. Fischer
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0046357
Abstract: Tel2, a protein conserved from yeast to vertebrates, is an essential regulator of diverse cellular processes including telomere maintenance, DNA damage checkpoints, DNA repair, biological clocks, and cell signaling. The Drosophila Tel2 protein is produced as a translational fusion with EpsinR, a Clathrin adapter that facilitates vesicle trafficking between the Golgi and endosomes. EpsinR and Tel2 are encoded by a Drosophila gene called lqfR. lqfR is required for viability, and its specific roles include cell growth, proliferation, and planar cell polarity. We find that all of these functions of lqfR are attributed entirely to Tel2, not EpsinR. In addition, we find that Drosophila LqfR/Tel2 is a component of one or more protein complexes that contain E-cadherin and Armadillo. Moreover, Tel2 modulates E-cadherin and Armadillo cellular dynamics. We propose that at least one of the functions of Drosophila Tel2 is regulation of Wingless signaling.
The role that oilseeds, including new hi-oleic varieties can play in improving the profile of fat intake by the UK population  [PDF]
Janice Irene Harland
Agricultural Sciences (AS) , 2014, DOI: 10.4236/as.2014.53024

The production in the EU of the oilseeds, rapeseed and sunflower, has increased dramatically over the last 20 years. Much of the oil produced after crushing is used for culinary purposes; this enhanced intake of vegetable oil has led to a substantial change of fatty acid (FA) supply. This has been conclusively demonstrated by taking the UK oil supply data and by use of the FA profile of the key oils converting the supply data into a FA profile of the UK market place for 2008-2012. The most marked changes are a reduction in saturated fat (SFA) and an increase in monounsaturated fatty acids (MUFA) available for consumption. Furthermore the introduction of varieties of hi-oleic sunflower oil can further affect the market FA profile. The fat profiles of rapeseed and sunflower oils are considered healthy and they can have a positive impact when included in the diet, particularly as a replacement for oils or fats rich in SFA. In the UK and much of Europe, adult SFA intake continues to exceed recommendations. While reductions in the UK population’s SFA intake have occurred over the last 20 years, these are modest and it may be timely to identify ways in which SFA intake can be further reduced. To do this, the UK market FA supply data has been analysed alongside the profile of FA intake from adults recording their intake in national dietary surveys in order to identify if the market supply affects overall FA consumption. There is an indication that market oil supply is reflected in adults dietary intake of the main groups of FA. Consequently changes made to the oil profile of oilseeds by plant breeders and use of the resulting healthier oils by food manufacturers could have important roles to play in helping adults to achieve the recommended intake of SFA and also improve the overall fat quality in their diet leading to enhanced long-term health and well-being. Thus changes made in pri

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