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Search Results: 1 - 10 of 403821 matches for " Janess M. Mendoza "
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Elucidating the Kinetics of Expression and Immune Cell Infiltration Resulting from Plasmid Gene Delivery Enhanced by Surface Dermal Electroporation
Janess M. Mendoza,Dinah H. Amante,Gleb Kichaev,Christine L. Knott,William B. Kiosses,Trevor R. F. Smith,Niranjan Y. Sardesai,Kate E. Broderick
Vaccines , 2013, DOI: 10.3390/vaccines1030384
Abstract: The skin is an attractive tissue for vaccination in a clinical setting due to the accessibility of the target, the ease of monitoring and most importantly the immune competent nature of the dermal tissue. While skin electroporation offers an exciting and novel future methodology for the delivery of DNA vaccines in the clinic, little is known about the actual mechanism of the approach and the elucidation of the resulting immune responses. To further understand the mechanism of this platform, the expression kinetics and localization of a reporter plasmid delivered via a surface dermal electroporation (SEP) device as well as the effect that this treatment would have on the resident immune cells in that tissue was investigated. Initially a time course (day 0 to day 21) of enhanced gene delivery with electroporation (EP) was performed to observe the localization of green fluorescent protein (GFP) expression and the kinetics of its appearance as well as clearance. Using gross imaging, GFP expression was not detected on the surface of the skin until 8 h post treatment. However, histological analysis by fluorescent microscopy revealed GFP positive cells as early as 1 h after plasmid delivery and electroporation. Peak GFP expression was observed at 24 h and the expression was maintained in skin for up to seven days. Using an antibody specific for a keratinocyte cell surface marker, reporter gene positive keratinocytes in the epidermis were identified. H&E staining of treated skin sections demonstrated an influx of monocytes and granulocytes at the EP site starting at 4 h and persisting up to day 14 post treatment. Immunological staining revealed a significant migration of lymphocytic cells to the EP site, congregating around cells expressing the delivered antigen. In conclusion, this study provides insights into the expression kinetics following EP enhanced DNA delivery targeting the dermal space. These findings may have implications in the future to design efficient DNA vaccination strategies for the clinic.
HIV-1 Env DNA Vaccine plus Protein Boost Delivered by EP Expands B- and T-Cell Responses and Neutralizing Phenotype In Vivo
Kar Muthumani, Megan C. Wise, Kate E. Broderick, Natalie Hutnick, Jonathan Goodman, Seleeke Flingai, Jian Yan, Chaoran B. Bian, Janess Mendoza, Colleen Tingey, Christine Wilson, Krzysztof Wojtak, Niranjan Y. Sardesai, David B. Weiner
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0084234
Abstract: An effective HIV vaccine will most likely require the induction of strong T-cell responses, broadly neutralizing antibodies (bNAbs), and the elicitation of antibody-dependent cellular cytotoxicity (ADCC). Previously, we demonstrated the induction of strong HIV/SIV cellular immune responses in macaques and humans using synthetic consensus DNA immunogens delivered via adaptive electroporation (EP). However, the ability of this improved DNA approach to prime for relevant antibody responses has not been previously studied. Here, we investigate the immunogenicity of consensus DNA constructs encoding gp140 sequences from HIV-1 subtypes A, B, C and D in a DNA prime-protein boost vaccine regimen. Mice and guinea pigs were primed with single- and multi-clade DNA via EP and boosted with recombinant gp120 protein. Sera were analyzed for gp120 binding and induction of neutralizing antibody activity. Immunization with recombinant Env protein alone induced low-titer binding antibodies with limited neutralization breath. In contrast, the synthetic DNA prime-protein boost protocol induced significantly higher antibody binding titers. Furthermore, sera from DNA prime-protein boost groups were able to neutralize a broader range of viruses in a panel of tier 1 clade B viruses as well as multiple tier 1 clade A and clade C viruses. Further investigation of synthetic DNA prime plus adaptive EP plus protein boost appears warranted.
Evaluación de la ocurrencia de sequía en localidades de Venezuela
Mendoza,N; Puche,M;
Revista de la Facultad de Agronomía , 2007,
Abstract: drought can be defined as an imbalance between water supply and demand. meteorological drought comes about when water deficit is due to below average rainfalls compared with the historical records. meteorological drought can be characterized through its intensity, duration and spatial coverage. first of all a drought index should be chosen. the standardized precipitation index (spi) and deciles were estimated in order to define meteorological drought in venezuela. from a total of 85 places, 67 located to the north of the orinoco river had 49 years of monthly rainfall records (1950-1998) whereas only 30 years (1969-1998) were available for the 18 locations to the south. for each station, year and month; monthly, three month and twelve month rainfall totals were obtained and both drought indices were estimated for these series. once the indices were calculated, the percentage of stations with drought was determined for each time scale. years with high percentage of stations were mappings to know which zones of the country are the affected ones. the percentage of stations with drought varies with the index and the chosen time scale. deciles show more drought cases than spi. year 1973 was the only one showing a high percentage of stations with drought for both indexes and all time scales. a pattern on sp was not detected along this year.
Democracy, Socialism, and Post Revolutionary States: Problems in Theory and Reality
Amado M. Mendoza Jr.
Kasarinlan : Philippine Journal of Third World Studies , 1989,
Abstract: The paper is a discussion on the difficulties involved in the theoretical specification of socialism, what with contrasting tendencies of idealizing it or apologizing for so-called actually existing socialism. An examination of classical Marxism's discourse on the central problem of human alienation and bondage and how it could be superseded by the socialist project through an eventual unity of state and civil society follows with serious questions being raised whether civil society can indeed be the real political state (with its attendant ramifications) or whether the political state continues to exist during the socialist transition to mediate between factions of civil society, whether organized political parties or movements or as popular organizations. What is in question here is the claim that the state as such will eventually disappear along with politics (understood as the governance of men) to be replaced simply by the "administration of things" in communism. Alongside the idea of the eventual withering away of the state is that of the strategic abolition of the social division of labor in communism which is again examined critically in the light of recent assertions that state and social division of labor, especially between manual and mental labor, nurture each other, that the full emancipation of labor from the prevailing social division of labor not only requires a long period of cultural and intellectual development, which in turn must be managed by a body such as the state or any other revolutionary change agent, such as the Party or any other entity; and that the state has non-suppressive, socially necessary functions which could and should be exercised in the period of post-revolutionary socialist construction. The last theoretical section discusses the problems of democratization and citizens' participation in socialist politics focusing attention on the familiar "free rider" problem endemic in all collective endeavors and how it could be resolved.
Democratization and Economic Growth: What's in Store for South Korea?
Amado M. Mendoza Jr.
Kasarinlan : Philippine Journal of Third World Studies , 1996,
Abstract: Is democracy incompatible with economic growth? To a number of states which have embarked on a process of transition from an authoritarian, state-led economy to a democratic, market-led one, the process of unraveling this thesis on “transitional incompatibility” has not been an easy one. A case in point is South Korea — the Economic dragon of East Asia — which, after having attained a considerable level of growth in the late 1970s, largely under the auspices of authoritarian rule, made headway towards democratization. Studies in recent years, however, show how its economy has been decelerating since the onset of democracy, even as others consider the converse true with economic growth and market forces undermining democratization. At the center of the paradox are the chaebols —seekers of state-assigned rents committed to the pursuit of national development, on the one hand, and political patrons who finance politicians with business money during elections, on the other. In the end, one finds consolation in the thesis that at per capita GNP levels as South Korea’s democracy is most likely to survive. The quality of democracy is, however, an entirely different
Evaluación de la ocurrencia de sequía en localidades de Venezuela Drought occurrence evaluation in localities of Venezuela
N Mendoza,M Puche
Revista de la Facultad de Agronomía , 2007,
Abstract: La sequía puede definirse como un desequilibrio entre la oferta y la demanda de agua. Cuando el déficit de agua es producto de una disminución en las lluvias comparada con el registro histórico se denomina sequía meteorológica. La sequía meteorológica puede ser caracterizada a través de su intensidad, magnitud y duración. Para establecer esa caracterización es necesario definir sequía a través de índices. En este trabajo se utilizaron el índice Deciles y el índice de Precipitación Estandarizada (SPI) con datos de precipitación de Venezuela agrupados en tres períodos (mensual, trimestral y anual), en 64 estaciones al norte del Orinoco con un período común de registro de 49 a os (1950-1998) y en 18 estaciones al sur con período de registro de 30 a os (1969-1998). Para cada índice y período de agrupación se determinó el porcentaje de estaciones con sequía (PE) y para los a os de gran PE, se realizaron mapas para detectar el patrón espacial de las áreas afectadas por sequía. El PE varía según el índice y el período de agrupación. El índice Deciles arroja mayor cantidad de casos y por ello mayor PE que el SPI. El a o 1973 fue el único que presentó gran PE para todos los períodos de agrupación en ambos índices. No se detectó un patrón en el PE a lo largo de ese a o. Drought can be defined as an imbalance between water supply and demand. Meteorological drought comes about when water deficit is due to below average rainfalls compared with the historical records. Meteorological drought can be characterized through its intensity, duration and spatial coverage. First of all a drought index should be chosen. The Standardized Precipitation Index (SPI) and Deciles were estimated in order to define meteorological drought in Venezuela. From a total of 85 places, 67 located to the north of the Orinoco River had 49 years of monthly rainfall records (1950-1998) whereas only 30 years (1969-1998) were available for the 18 locations to the South. For each station, year and month; monthly, three month and twelve month rainfall totals were obtained and both drought indices were estimated for these series. Once the indices were calculated, the percentage of stations with drought was determined for each time scale. Years with high percentage of stations were mappings to know which zones of the country are the affected ones. The percentage of stations with drought varies with the index and the chosen time scale. Deciles show more drought cases than SPI. Year 1973 was the only one showing a high percentage of stations with drought for both indexes and all time scales. A pattern on
Modelación del proceso de postcombustión en un horno de reducción de níquel
Ramírez Mendoza, M.
Revista de Metalurgia , 2002,
Abstract: Very complex multivariable processes are carried out in multi-hearth furnaces which can only be modeled with great uncertainty. A sketch of the experiments carried out in an industrial roaster to obtain a dynamic model of the postcombustion process for automatic control design purposes is presented in this paper. The appropriate development of this process influences decisively in the achievement of a bigger efficiency and a good temperature profile. The classic validation tests produced excellent results and the model obtained represented the behavior of the process satisfactorily. This made possible the simulation of the automatic control system that will be used to maintain the temperature in the established values and to diminish the environmental contamination. En los hornos de hogares múltiples se llevan a cabo procesos multivariables muy complejos que solamente pueden modelarse con gran incertidumbre. En este artículo se hace un esbozo de los experimentos realizados en un horno industrial para la obtención de un modelo dinámico del proceso de postcombustión, para propósitos de dise o de sistemas de control automático. El desarrollo adecuado de este proceso debe influir, decisivamente, en el logro de un mayor rendimiento y de un perfil de temperatura óptimo en el equipo. Las pruebas de validación clásicas arrojaron excelentes resultados y el modelo obtenido representó satisfactoriamente el comportamiento del proceso, lo que posibilitó la simulación del sistema de control automático que se prevé utilizar para mantener la temperatura en los valores establecidos y disminuir la contaminación ambiental.
Dise os de sujeto único versus de grupo? Una antigua polémica con vigencia actual en investigación psicológica
Trujillo Mendoza, Humberto M.
Arbor : Ciencia, Pensamiento y Cultura , 2002,
Abstract: Not available Con este trabajo se pretende justificar que la consecución de la explicación-relación de fenómenos psicológicos trasciende del tipo de dise o que se aplique para la obtención de datos de interés investigador. De forma que se hace explícito cómo los distintos dise os explicativos, los grupales y los de sujeto único, implican formas distintas para llevar a efecto las acciones comparativas pero igual de rigurosas. Asimismo, se intenta demostrar que no tiene demasiado sentido entender que algunos de ellos son mejores que otros, sino que para su elección debe imperar el criterio de adecuación estructural y analítica a los fenómenos naturales que se pretenden constre ir. Para ello, tras una breve introducción se hace una exposición de ideas acerca del estado actual de la metodología en Psicología para finalmente llegar a una reconceptualización de los dise os de sujeto único. Reconceptualización ésta, llevada a cabo desde lo puramente estructural y no sólo desde lo absolutamente estadístico.
A Gauge Condition for Studying the Origin of Intrinsic Magnetospheres
M. Mendoza,J. Morales
Physics , 2004,
Abstract: We propose an analytical model based on the solution of the magnetohydrodynamics (MHD) equations for studying the origin of intrinsic magnetospheres. For this purpose, we reveal a new gauge condition for the electromagnetic vector potential, which eases the solution of such complex system of non-linear equations. Using this model, we analyse the deformation of the terrestrial magnetic field due to the presence of the solar wind. By comparing with experimental observations, we have found that the geometrical configuration of the magnetosphere, before, and after the magnetic field of the Earth started to deviate the solar wind, has not changed notably, and that the solar wind should have had a finite conductivity. This model can also be used to perform linear stability analysis of fluid and magnetic instabilities.
Lattice Boltzmann Model for Numerical Relativity
E. Ilseven,M. Mendoza
Physics , 2015,
Abstract: In the Bona-Masso formulation, Einstein equations are written as a set of flux conservative first order hyperbolic equations that resemble fluid dynamics equations. Based on this formulation, we construct a lattice Boltzmann model for Numerical Relativity. Our model is validated with well-established tests, showing good agreement with analytical solutions. Furthermore, we show that by increasing the relaxation time, we gain stability at the cost of losing accuracy, and by decreasing the lattice spacings while keeping a constant numerical diffusivity, the accuracy and stability of our simulations improves. Finally, in order to show the potential of our approach a linear scaling law for parallelisation with respect to number of CPU cores is demonstrated. Our model represents the first step in using lattice kinetic theory to solve gravitational problems.
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