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Search Results: 1 - 10 of 3329 matches for " Jaiesoon Cho "
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Production of an extracellular protease by an Antarctic bacterial isolate (Bacillus sp. JSP1)as a potential feed additive
Park,Inkyung; Cho,Jaiesoon;
Revista Colombiana de Ciencias Pecuarias , 2011,
Abstract: extracellular proteolytic activity was found in jsp1, an antarctic bacterial isolate. the strain was related to bacillus sp, based on 16s rrna gene sequence analysis. the jsp1 protease was partially purified by ammonium sulfate precipitation. optimal enzyme activity occurred at 40 c and ph 7.4. enzyme activity was significantly enhanced in the presence of mg2+ and ca2+ and was completely inactivated in presence of cu2+, zn2+, hg2+, edta and sds. the enzyme hydrolyzed casein the most effectively among the protein substrates tested. the enzyme also exhibited relatively high activity on keratin and gluten, and was active against peptidyl conjugates such asl-leu-p-nitroanilide and n-succinyl-l-phe-p-nitroanilide. this study suggests that jsp1 protease could be utilized as a potential environmentally-friendly feed additive in animal production.
Proteasa extracelular de un aislado bacteriano Antartico (Bacillus sp. JSP1) con uso potencial como aditivo alimenticio para animales Produ o de proteasa extracelular por bactérias antárcticas isoladas, Bacillus sp. JSP1 como um aditivo potencial em concentrado Production of an extracellular protease by an Antarctic bacterial isolate (Bacillus sp. JSP1)as a potential feed additive
Inkyung Park,Jaiesoon Cho
Revista Colombiana de Ciencias Pecuarias , 2011,
Abstract: Se reporta haber encontrado actividad proteolítica extracelular en una bacteria antártica denominada JSP1. Con base en el análisis de secuencia genética 16S ARNr, la cepa fué relacionada con Bacillus sp. La proteasa JSP1 fué parcialmente purificada por precipitación con sulfato de amonio. La actividad óptima de la enzima se produjo a 40 oC y pH 7,4. La actividad enzimática fue significativamente mayor en presencia de Mg2+ y Ca2+ y se inactivó completamente en presencia de Cu2+, Zn2+ , Hg2+ , EDTA y SDS. Entre todos los sustratos ensayados, el más eficientemente hidrolizado por la enzima fue la caseína. La enzima tuvo actividad relativamente alta sobre la queratina y el gluten, y participó activamente contra conjugados peptídicos como la L-Leu-p-nitroanilida y la N-succinil-L-fenilalanina-p-nitroanilida. La enzima podría ofrecer potencial para su uso como aditivo alimenticio ecológico en producción animal. A actividade proteolítica extracelular foi encontrada a partir de uma bactéria antárctica denominada JSP1, mediante uma análises de sequencia do gene 16S rRNA, a cepa foi relacionada para Bacillus sp. A proteasa JSP1 foi parcialmente purificada por precipita o com sulfato de am nia. Uma óptima actividade enzimática ocorreu em 40 oC e pH 7,4. A actividade da enzima foi significativamente maior na presen a de Mg2 + e Ca2 +, e foi completamente inactivada em presen a de Cu2 + Zn2 +, Hg2 +, EDTA e SDS. A enzima hidrolisada de caseína foi mais eficaz entre os substratos de proteína testados, apresentando maior actividade na queratina e no glúten e foi activo contra os peptidil conjugados como L-Leu-p Nitroanilida e N-succinil-L-Phe-p-Nitroanilida. A enzima pode ser utilizado como aditivo ambiental na alimenta o animal. Extracellular proteolytic activity was found in JSP1, an Antarctic bacterial isolate. The strain was related to Bacillus sp, based on 16S rRNA gene sequence analysis. The JSP1 protease was partially purified by ammonium sulfate precipitation. Optimal enzyme activity occurred at 40 C and pH 7.4. Enzyme activity was significantly enhanced in the presence of Mg2+ and Ca2+ and was completely inactivated in presence of Cu2+, Zn2+, Hg2+, EDTA and SDS. The enzyme hydrolyzed casein the most effectively among the protein substrates tested. The enzyme also exhibited relatively high activity on keratin and gluten, and was active against peptidyl conjugates such asL-Leu-p-Nitroanilide and N-Succinyl-L-Phe-p-Nitroanilide. This study suggests that JSP1 protease could be utilized as a potential environmentally-friendly feed additive in animal production.
Bacillus strains as feed additives: In vitro evaluation of its potential probiotic properties Bacilos utilizados como aditivos para piensos: Evaluación in vitro de sus potenciales propiedades probióticas Bacilos utilizados como aditivos para concentrados: Avalia o in vitro de suas propriedades probióticas
Jaekoo Lee,Inkyung Park,Yunjaie Choi,Jaiesoon Cho
Revista Colombiana de Ciencias Pecuarias , 2012,
Abstract: Background: much recent attention has been devoted to the genuine value of Bacillus species as multifunctional probiotic products, which produce various extracellular enzymes that enhance feed digestibility as well as many antimicrobial compounds for the purpose of improving animal performance. Objective: to describe novel, in vitro potential probiotic properties such as acid tolerance, bile tolerance, safety, and antimicrobial activity of mesophilic and psychrophilic Bacillus strains in conjunction with their extracellular enzymatic activities. Methods: four Bacillus strains (B. sp. T3, B. sp. T4, B. sp. SM2, and B. sp. JSP1) isolated from different sources were used. Strains were identified according to 16S rDNA sequences. Escherichia coli K88, E. coli O157:H7, Salmonella enteritidis KCCM 12021, Enterococcus faecalis, Listeria monocytogenes, and Staphylococcus aureus were used as indicator bacteria for the antimicrobial activity trial. Strains were activated and cultured in tryptic soy broth (pH 7.0) or broth solidified with 1.5% agar. Results: B. sp. JSP1 was fully resistant to both pH 2 and 3, whereas B. sp. SM2 showed relatively good viability at pH 3. All strains tolerated oxgall (0.3%) bile salt and were not cytotoxic to the HEK 293 human embryonic kidney cells. Three strains, except B. sp. T3, displayed differential production of extracellular enzymes including amylase, xylanase, cellulase, protease, phytase, and α-galactosidase. In particular, B. sp. SM2 inhibited six indicator pathogens: Escherichia coli K88, E. coli O157:H7, Salmonella enteritidis, Enterococcus faecalis, Listeria monocytogenes, and Staphylococcus aureus. Conclusion: the single use of B. sp. SM2 or the mixed use of the strain combined with acid or bile tolerant Bacillus strains secreting extracellular enzymes may be an alternative to antibiotics as a feed additive in farm animal production. Antecedentes: recientemente el valor de las especies de Bacillus como productos probióticos multifuncionales ha recibido bastante atención, debido a que estos producen varias enzimas extracelulares que potencian la digestibilidad de los alimentos, así como también compuestos antimicrobianos que mejoran el desempe o del animal. Objetivo: describir y evaluar potenciales propiedades probióticas ''in vitro'' -tales como acidez, tolerancia a la bilis, seguridad y actividad antimicrobiana- de cuatro cepas de Bacilos (B. sp. T3, B. sp. T4, B. sp. SM2 y B. sp. JSP1) aisladas de diferentes fuentes, en conjunción con sus actividades enzimáticas extracelulares. Métodos: se usaron cuatro cepas de Bacill
Posturographic Evaluation of Dizziness Complaining Patients under Suspicion to Develop Parkinson's Disease  [PDF]
Hyun Cho
Neuroscience & Medicine (NM) , 2011, DOI: 10.4236/nm.2011.21004
Abstract: Although the typical clinical signs of Parkinson disease (PD) are tremor, rigidity, bradykinesia, and postural instability, PD is preceded by a preclinical phase during which neuronal degeneration develops without typical symptoms. More general nonspecific symptoms including dizziness have also been described to predate the typical PD signs for several years. All subjects were selected among patients in the Willis Hospital (Pusan, South of Korea), with complaints of diz-ziness from September 2009 to September 2010 and the baseline neurological screening and clinical ENT examination, to which the results were within the normal range. At baseline, 113 participants underwent neurological screening and provided information on dizziness. Of those participants, 103 were enrolled including 63 subjects in the control group. We used posturography. It allows quantitative assessment of vestibular-spinal component of body balance. The parame-ter of average speed of pressure center displacement to the lateral plan (VMX) and antero-posterior plan (VMY), which presented statistically significant differences between the groups except VMX with closed eyes. (p = 0.008 and p = 0.012, with closed eyes). With open eyes, only VMY showed significant difference between the groups (p = 0.010). In this study, the patients with dizziness and subjective complaints related to typical clinical signs of PD complaints presented higher instability in the orthostatic position than the control group of patients with dizziness and without such complaint. It could suggest that dizziness may be one symptom of preclinical PD and progress to overt postural instability. It is believed that a stepwise approach with a simple and inexpensive initial screening test of preclinical PD is required.
To Open or Not to Open: Korean Rice Market  [PDF]
Youngjeen Cho
Beijing Law Review (BLR) , 2014, DOI: 10.4236/blr.2014.54023
Abstract: Although the “tariff only” principle was adopted in the Uruguay Round (UR) negotiations on agricultural products, Korea was able to apply special treatment to its rice and postpone tariffication of rice imports. After two decades of delay, the Korean government officially announced its decision to open its rice market beginning in 2015. This paper examines whether it would be legally possible for the Korean government to postpone the tariffication of rice imports beyond 2014. In order to address the question, this paper outlines the status of Korean rice in the WTO for the last twenty years. Then, it analyzes the arguments of those who are against tarifficating rice and of those who are in support of it. Based on the analysis, it concludes that Korea cannot postpone tariffication under Annex 5 of the Agreement on Agriculture. While it may request for a waiver under Article IX of the WTO Agreement, a careful and thorough examination is required to decide whether to postpone the tariffication beyond 2014.
Revisiting WTO Fisheries Subsidies Negotiations  [PDF]
Youngjeen Cho
Beijing Law Review (BLR) , 2015, DOI: 10.4236/blr.2015.61002
Abstract: Fisheries subsidies negotiation was added to the negotiation agenda of the DDA largely by the concern for the depletion of fishery stocks. Some members, in particular, the Friends of Fish group countries, claimed that over-fishing and overcapacity caused by various fisheries subsidies program have resulted in the depletion of fishery stocks. Therefore, they argued that new disciplines should be introduced to address the situation. In 2007, after more than five years of negotiations, the chairman of the Rules Negotiations Group circulated a consolidated text. However, the negotiations have hardly made progress since then. This paper examines whether the Rule Negotiating Group chair’s text is legally and practically appropriate to serve as a basis for the fisheries subsidies negotiations. In order to address the question, this paper presents the brief history of fisheries subsidies negotiations at the WTO. Then, it analyzes critical issues of the Rule Negotiating Group chair’s text. It also explores the consolidated text of the TPP negotiations. Based on the analysis, it concludes that the basic concept and the principle of the chair’s text are inconsistent with those of the ASCM, and that the structure of the text does not conform those of the ASCM and other WTO agreements. Likewise, the TPP text bears similar problems. Therefore, it is highly questionable whether WTO members and TPP parties can actually achieve the goal to conserve fishery resources. WTO members as well as TPP parties should try to address these problems, so that they can make real progress in negotiations.
Targeting Phosphodiesterase 4 to Block the Link between Acute Exacerbation of Chronic Obstructive Pulmonary Disease and the Metabolic Complications  [PDF]
Eric Cho
Journal of Biosciences and Medicines (JBM) , 2015, DOI: 10.4236/jbm.2015.311007
Abstract: The metabolic disorders such as obesity and diabetes are found to be more frequent in chronic obstructive pulmonary disease (COPD). The chronic systemic inflammation orchestrated by macrophages constitutes one critical pathophysiological process underlying both acute exacerbation of COPD (AECOPD) and its metabolic complications such as obesity and diabetes. The cyclic adenosine monophosphate (cAMP) signaling controlled by phosphodiesterase (PDE) 4 is a pivotal intracellular modulator for macrophages functions in immune inflammatory response underlying AECOPD as well as obesity and diabetes. Targeting PDE4/cAMP signaling has been suggested to be effective in treating AECOPD or the metabolic disorders of obesity and diabetes. It is therefore reasonable to hypothesize that the chronic systemic inflammation can be a critical link between AECOPD and the metabolic disorders and targeting the PDE4/cAMP signaling can be effective to block this link between AECOPD and the associated metabolic complications.
The Concept of “Developing Countries” in the Context of the WTO Fisheries Subsidies Negotiation  [PDF]
Youngjeen Cho
Beijing Law Review (BLR) , 2018, DOI: 10.4236/blr.2018.92010
Abstract: This paper explores the concept of developing countries in the context of the WTO DDA fisheries subsidies negotiations. It raises a question whether the concept of developing countries that is widely used in other WTO agreement should be applicable to the new disciplines on fisheries subsidies. In order to address the question, this paper outlines the history of the DDA fisheries subsidies negotiations and discusses the goal of fisheries subsidies negotiations. Then, it presents the concept of developing countries in the GATT, the WTO and other international institutions, and finds that it is almost exclusively based on economic indicators. Then, it reviews global fish production and the amount of global fisheries subsidies. On the basis of its analysis, this paper finds that, in order to achieve the proposed goal of the new disciplines on fisheries subsidies, and to strike a balance between the environmental and the developmental dimensions of fisheries subsidies, the concept of “developing countries” in the context of fisheries subsidies should be different from that in other WTO agreements. It should not include countries that have leading fishing industry for which the governments provide large amount of subsidies, in particular, fishing capacity-enhancing subsidies.
Work-Family Conflict Influences on Female’s Career Development through Career Expectation  [PDF]
Wei Wang, Taejun Cho
Journal of Human Resource and Sustainability Studies (JHRSS) , 2013, DOI: 10.4236/jhrss.2013.13007
Abstract: Nowadays, women are still underrepresented in the higher positions in most organizations. Some crucial questions are whether family responsibilities affect female career development and career expectation influences the relationship between work and family conflict and career development of women or not. Through surveying the female teachers in Lin Yi University and Shandong Medical College, this article wants to know the correct relationship among work and family conflict, career expectations and career developments for women.
An Integrative Framework for Customizations on Satisfaction: The Case of an Online Jewelry Business in China  [PDF]
Vincent Cho, Candy Lau
Journal of Service Science and Management (JSSM) , 2014, DOI: 10.4236/jssm.2014.72013
Abstract:

Upon the technological advancement of production flexibility, supply chain agility, and customer behavioral tracking, many online businesses now develop their customization strategies. In this regard, this study proposes an integrative framework for customization of product information, transaction handling, product attribute, and sales’ service. We are interested in how these customizations affect customer satisfaction. From our web-based survey on a renowned online jewelry company in China, we collected 1383 responses from its customer database. Our findings showed that information, product and sales’ service customizations have significant influence on customer satisfaction. Moreover, ordinary members as compared with VIP members feel more satisfied once they receive customized services.

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