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Search Results: 1 - 10 of 606 matches for " Jaeduk Goh "
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The PEX7-Mediated Peroxisomal Import System Is Required for Fungal Development and Pathogenicity in Magnaporthe oryzae
Jaeduk Goh, Junhyun Jeon, Kyoung Su Kim, Jongsun Park, Sook-Young Park, Yong-Hwan Lee
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0028220
Abstract: In eukaryotes, microbodies called peroxisomes play important roles in cellular activities during the life cycle. Previous studies indicate that peroxisomal functions are important for plant infection in many phytopathogenic fungi, but detailed relationships between fungal pathogenicity and peroxisomal function still remain unclear. Here we report the importance of peroxisomal protein import through PTS2 (Peroxisomal Targeting Signal 2) in fungal development and pathogenicity of Magnaporthe oryzae. Using an Agrobacterium tumefaciens-mediated transformation library, a pathogenicity-defective mutant was isolated from M. oryzae and identified as a T-DNA insert in the PTS2 receptor gene, MoPEX7. Gene disruption of MoPEX7 abolished peroxisomal localization of a thiolase (MoTHL1) containing PTS2, supporting its role in the peroxisomal protein import machinery. ΔMopex7 showed significantly reduced mycelial growth on media containing short-chain fatty acids as a sole carbon source. ΔMopex7 produced fewer conidiophores and conidia, but conidial germination was normal. Conidia of ΔMopex7 were able to develop appressoria, but failed to cause disease in plant cells, except after wound inoculation. Appressoria formed by ΔMopex7 showed a defect in turgor generation due to a delay in lipid degradation and increased cell wall porosity during maturation. Taken together, our results suggest that the MoPEX7-mediated peroxisomal matrix protein import system is required for fungal development and pathogenicity M. oryzae.
Homeobox Transcription Factors Are Required for Conidiation and Appressorium Development in the Rice Blast Fungus Magnaporthe oryzae
Seryun Kim,Sook-Young Park,Kyoung Su Kim,Hee-Sool Rho,Myoung-Hwan Chi,Jaehyuk Choi,Jongsun Park,Sunghyung Kong,Jaejin Park,Jaeduk Goh,Yong-Hwan Lee
PLOS Genetics , 2009, DOI: 10.1371/journal.pgen.1000757
Abstract: The appropriate development of conidia and appressoria is critical in the disease cycle of many fungal pathogens, including Magnaporthe oryzae. A total of eight genes (MoHOX1 to MoHOX8) encoding putative homeobox transcription factors (TFs) were identified from the M. oryzae genome. Knockout mutants for each MoHOX gene were obtained via homology-dependent gene replacement. Two mutants, ΔMohox3 and ΔMohox5, exhibited no difference to wild-type in growth, conidiation, conidium size, conidial germination, appressorium formation, and pathogenicity. However, the ΔMohox1 showed a dramatic reduction in hyphal growth and increase in melanin pigmentation, compared to those in wild-type. ΔMohox4 and ΔMohox6 showed significantly reduced conidium size and hyphal growth, respectively. ΔMohox8 formed normal appressoria, but failed in pathogenicity, probably due to defects in the development of penetration peg and invasive growth. It is most notable that asexual reproduction was completely abolished in ΔMohox2, in which no conidia formed. ΔMohox2 was still pathogenic through hypha-driven appressoria in a manner similar to that of the wild-type. However, ΔMohox7 was unable to form appressoria either on conidial germ tubes, or at hyphal tips, being non-pathogenic. These factors indicate that M. oryzae is able to cause foliar disease via hyphal appressorium-mediated penetration, and MoHOX7 is mutually required to drive appressorium formation from hyphae and germ tubes. Transcriptional analyses suggest that the functioning of M. oryzae homeobox TFs is mediated through the regulation of gene expression and is affected by cAMP and Ca2+ signaling and/or MAPK pathways. The divergent roles of this gene set may help reveal how the genome and regulatory pathways evolved within the rice blast pathogen and close relatives.
The need for evidence-based aesthetic dermatology practice
Goh C
Journal of Cutaneous and Aesthetic Surgery , 2009,
Mechanism in allergic contact dermatitis
Goh C
Indian Journal of Dermatology, Venereology and Leprology , 1991,
Relationship between Video Game Violence and Long-Term Neuropsychological Outcomes  [PDF]
Yoshiyuki Tamamiya, Goh Matsuda, Kazuo Hiraki
Psychology (PSYCH) , 2014, DOI: 10.4236/psych.2014.513159
Abstract: The current study examined the long-term effects of video game violence on aggressiveness and facial expression recognition using multiple measures. In Experiment 1, participants unfamiliar with video games were randomly assigned to play a violent or nonviolent video game for four weeks. Before and after the game play interval, event-related potentials (ERP) evoked by facial expressions were recorded, and aggressiveness was measured with a questionnaire. Results showed that playing a violent video game delayed peak latency of a positive component of the ERP evoked by angry faces and increased aggressiveness among male participants. Experiment 2 included a 3-month follow-up assessment. Results showed preservation of delayed neural activity, while levels of aggressiveness diminished to some extent. These findings highlight differential aspects regarding the long-term effects of playing a violent video game: more enduring for facial expression recognition and short-lived for aggressiveness.
Supply Chain Orchestration Leveraging on MNC Networks and Local Resources: Approach Strategies  [PDF]
Zhengping Li, Robert De Souza, Mark Goh
Journal of Service Science and Management (JSSM) , 2016, DOI: 10.4236/jssm.2016.94036
Abstract: Competition is becoming more global and is between the networks of businesses rather than between the firms. Operating in such an increasing networked environment, companies have to innovate their business, either be orchestrators or be orchestrated. This exploratory paper proposes an overarching Supply Chain Orchestration (SCO) framework which leverages on the capabilities of the MNC’s networks and the local Logistics Service Providers (LSPs) at a strategic level. This framework comprises integrated components including orchestrated Supply Chain Network (SCN), the relationship model of the actors, a “Win-for-All” pro-orchestration model, and the collaboration platform for the LSPs. Specifically, the paper proposes a practical approach for upgrading the local industry through a virtuous model whereby the MNCs, LSPs, and government agencies collaborate to create a pro-orchestration environment for greater value adding. Using a semi-structured interview approach, key players across the logistics sector in Singapore were interviewed. The approach strategies for realizing this orchestration are investigated and the implications are discussed through a few application scenarios.
User Goals, Behaviours and Attitudes: Developing Web User Personas of Art and Design Students  [PDF]
Chu Hiang Goh, Nurul Hanim Romainoor
Art and Design Review (ADR) , 2019, DOI: 10.4236/adr.2019.71001
Abstract: User personas are one of the most understandable deliverables which used to improve user experience and enhance design communication in web design process. Myriad studies and design research were conducted in this area which was largely based on qualitative research methods. This posted a challenge empirically in the validation of the personas created. To set on this gap, a qualitative-quantitative validation method was adapted with the focus in the three main attributes of personas: user goals, behaviours, and attitudes in this research to create student web user personas in the School of The Arts, Universiti Sains Malaysia web site. As an outcome, a set of art and design student web user personas were presented.
On Mining Movement Pattern from Mobile Users
David Taniar,John Goh
International Journal of Distributed Sensor Networks , 2007, DOI: 10.1080/15501320601069499
Abstract: In the era in which activities performed by mobile users are tracked through various sensing mechanisms, the movement data collected through these sensors is submitted into a data mining algorithm in order to determine the movement pattern. The movement pattern refers to the pattern that mobile users generally take to move from one base location to another base location through multiple intermediate locations. This paper provides a proposal and case study on how the movement pattern can be extracted from mobile users through transforming the user movement database to the location movement database and subsequently transferred to an algorithm Apriori-like movement pattern (AMP) and movement tree (M-tree). The result is a list of sequences in which mobile users frequently go through that which satisfies min-support and min-confidence. The result of this movement pattern mining exercise opens up a new future for the prediction of the movement for the individual mobile user.
Plasmid selection in Escherichia coli using an endogenous essential gene marker
Shan Goh, Liam Good
BMC Biotechnology , 2008, DOI: 10.1186/1472-6750-8-61
Abstract: The new cloning vector, pFab, enabled selection by triclosan at 1 μM. Interestingly, pFab out-performed the parent pUC19-ampicillin system in cell growth, plasmid stability and plasmid yield. Also, pFab was toxic to host cells in a way that was reversed by triclosan. Therefore, pFab and triclosan are toxic when used alone but in combination they enhance growth and plasmid production through a gene-inhibitor interaction.The fabI-triclosan model system provides an alternative plasmid selection method based on essential gene over-expression, without the use of antibiotic-resistance genes and conventional antibiotics.Antibiotic resistance marker genes are commonly used to select and maintain recombinant bacteria in the presence of antibiotics. However, the use of antibiotics is undesirable for manufacturing gene therapy products [1,2]. Also, the introduction of antibiotic resistance genes into biohazardous strains is not recommended [3], and antibiotic selection fails in bacteria that are naturally resistant [3,4]. Finally, the use of antibiotics can be costly in industrial scale production, especially in the case of enzymatic depletion of antibiotic during culture. An alternative system should avoid antibiotic resistance markers and therapeutic antibiotics, be cost effective, but still be convenient, robust and flexible.A variety of strategies for antibiotic-free selection have been developed, but none are widely used in bacteria. The first reported non-antibiotic system involves an auxotrophic bacterial strain and complementation using a plasmid-encoded biosynthesis gene, such that only transformants grow on defined media lacking the nutrient [5]. Another system is termed repressor-titration, where the lac operator functions as the vector-borne selection marker, which de-represses a modified chromosomal essential gene [6]. A third general approach is to alter the expression of growth essential genes using synthetic [7] or expressed antisense sequences [8]. Unfortunate
Investigational new drugs in the treatment of inflammatory bowel disease: a review
Imogen Williams, Jason Goh
Journal of Experimental Pharmacology , 2011, DOI: http://dx.doi.org/10.2147/JEP.S7806
Abstract: vestigational new drugs in the treatment of inflammatory bowel disease: a review Review (3953) Total Article Views Authors: Imogen Williams, Jason Goh Published Date February 2011 Volume 2011:3 Pages 13 - 19 DOI: http://dx.doi.org/10.2147/JEP.S7806 Imogen Williams, Jason Goh GI Unit, Queen Elizabeth Hospital Birmingham, Edgbaston, Birmingham, England, UK Abstract: The unraveling of the immuno-pathobiology of inflammatory bowel disease (IBD) in the past three decades has ushered in a new era of translational medicine. The biotechnology revolution has resulted in a paradigm shift in how clinicians view and treat IBD. Anti-tumor necrosis factor (TNF)-α strategies using infliximab and adalimumab currently dominate the therapeutic arena. Better understanding of how these biologicals work is driving the quest for loftier therapeutic goals of achieving mucosal healing, sustaining deep remission, and even modifying the natural history of IBD. However, not all patients respond to anti-TNF drugs. Immune-mediated adverse reactions and loss of efficacy with time also limit their use. There are many investigational drugs undergoing active clinical trials. Many have not fulfilled their early promises but some are potentially making the transition from bench to trial and to the bedside in the near future. Clinicians and investigators need to underpin our excitement with caution for the unknown long-term consequences of modulating cytokines and selective adhesion molecules in human. Here we provide an overview of investigational new drugs and other therapeutic strategies currently undergoing clinical trials in IBD.
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