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Search Results: 1 - 10 of 131940 matches for " JOSé JUVENAL; GUERREIRO DA SILVA "
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Genetic polymorphism of GSTM1 in women with breast cancer and interact with reproductive history and several clinical pathologies
JOSé JUVENAL LINHARES,ISMAEL DALE COTRIM GUERREIRO DA SILVA,NAIARA C NOGUEIRA DE SOUZA,EMMANUELLE COELHO NORONHA
Biological Research , 2005,
Abstract: Due to the conflicting results regarding the association between breast cancerand the GSTM1 null mutation, our aim was to research this associationin a Brazilian population and correlations withsmoking, reproductive history and several clinical pathologies. A case-control study was performed on 105 women with breast cancer and 278 controls. Extraction of DNA was accomplished according to the protocol of the GFX kit and polymorphism analysis by the PCR technique. The control and experimental groups were compared and statistical analysis assessed by X2 or Fisher's exact test. The deletion in the GSTM1 gene in the breast cancer group had a prevalence of 32 (30.4%) individuals with the presence of null mutation. In the control group, the null mutation was present in 104 (37.4%) women. Upon comparison of the two groups, no statistically significant difference of the GSTM1 gene was observed, with an odds ratio (OR) of 0.74, 95%, confidence interval (CI) 0.45 - 1.20, p = 0.277. The results conclusively show that singlegene GSTM1 polymorphisms do not confer a substantial risk of breastcancer to its carriers. Furthermore, in this study no correlation was found between GSTs andsmoking, reproductive history and several clinical pathologies with respect to cancer risk.
Polimorfismo do gene do receptor de progesterona (PROGINS) em mulheres com cancer de mama: estudo caso-controle
Linhares, José Juvenal;Silva, Ismael Dale Cotrim Guerreiro da;Souza, Naiara Correa Nogueira de;Noronha, Emmanuelle Coelho;Ferraro, Odair;Baracat, Fausto Farah;
Revista Brasileira de Ginecologia e Obstetrícia , 2005, DOI: 10.1590/S0100-72032005000800007
Abstract: purpose: to analyze the correlation between progins polymorphism and breast cancer. methods: a case-control study was carried out from april to october 2004. the genotypes of 50 women with breast cancer and 49 healthy women were analyzed. the 306-base pair alu insertion polymorphism in the g intron of progesterone receptor gene was detected by polymerase chain reaction and analyzed on 2% agarose gel stained with ethidium bromide. the control and experimental groups were compared regarding genotypes using the statistical epi-info 6.0 program and for frequencies the exact fisher test or c2 test were used. p value smaller p than 5% was considered to be significant. results: in relation to progins we found in the studied population a prevalence of 62 (62.6%) wild homozygous, 35 (35.3%) heterozygous individuals and two (2.1%) cases with the presence of the mutation. regarding progins polymorphism, significance was not evidenced when cases and controls were compared, as related to homozygosis (62 vs 65.3%), heterozygosis (36 vs 34,6%) or the mutation (2.0 vs 2.1%), with p=0.920 (or=1.01), 0.891 (or=1.06), and 0.988 (or=1.10), respectively. conclusions: the results show that single-gene progins polymorphism does not confer a substantial risk of breast cancer to its carriers.
Genetic polymorphism of GSTM1 in women with breast cancer and interact with reproductive history and several clinical pathologies
LINHARES,JOSé JUVENAL; GUERREIRO DA SILVA,ISMAEL DALE COTRIM; NOGUEIRA DE SOUZA,NAIARA C; COELHO NORONHA,EMMANUELLE; FERRARO,ODAIR; DE CARVALHO,CRISTINA VALLETA; CHADA BARACAT,EDMUND; FARAH BARACAT,FAUSTO;
Biological Research , 2005, DOI: 10.4067/S0716-97602005000200017
Abstract: due to the conflicting results regarding the association between breast cancer and the gstm1 null mutation, our aim was to research this association in a brazilian population and correlations with smoking, reproductive history and several clinical pathologies. a case-control study was performed on 105 women with breast cancer and 278 controls. extraction of dna was accomplished according to the protocol of the gfx? kit and polymorphism analysis by the pcr technique. the control and experimental groups were compared and statistical analysis assessed by x2 or fisher's exact test. the deletion in the gstm1 gene in the breast cancer group had a prevalence of 32 (30.4%) individuals with the presence of null mutation. in the control group, the null mutation was present in 104 (37.4%) women. upon comparison of the two groups, no statistically significant difference of the gstm1 gene was observed, with an odds ratio (or) of 0.74, 95%, confidence interval (ci) 0.45 - 1.20, p = 0.277. the results conclusively show that single gene gstm1 polymorphisms do not confer a substantial risk of breast cancer to its carriers. furthermore, in this study no correlation was found between gsts and smoking, reproductive history and several clinical pathologies with respect to cancer risk.
Prevalence of Human Leukocyte Antigen HLA-B*5701 in HIV-1 Infected Individuals in Brazil  [PDF]
Claudinéia de Araújo, Cristina Valetta de Carvalho, Miriam Estela de Souza Freire, Amanda Yamaguti, Ivens Cuiabano Scaff, Fernando José de Souza, Flávia Galindo Silvestre Silva, Ricardo Sobhie Diaz, Ismael Dale Cotrim Guerreiro da Silva
Open Journal of Genetics (OJGen) , 2014, DOI: 10.4236/ojgen.2014.41008
Abstract:

This study was designed to establish the prevalence of HLA-B*5701 at HIV-1 infected individuals in Brazil. A total of 517 consecutive individuals were followed in this study from February 2009 through July 2011. The presence of HLA-B*5701 was determined by Nested-PCR with HLA-B*57 and HLA-B*5701 sequence-specific primers (PCR-SSP). The expression of HLA-B*57 was negative in the 385 (74.5%) and positive in the 103 (19.9%) of infected individuals. Among these, the expression of HLA-B5701 was positive in the 29 (5.6%) of individuals. No demographic or ethnic differences were found between HLA-B*57/HLA-B*5701 HIV-1 negative patients, with a prevalence of Caucasians (57.1%) individuals. During the period of study, 68 patients were submited to an abacavir contain- ing regimen. The HLA-B*5701 allele was observed in 7 (10.3%) patients, with a significant incidence of Hypersensitivity reactions at 4 of them (p < 0.001). Conclusions: Although Brazilian population consists of a mixture of individuals of Caucasian, African and Native American genetic background, prevalence of HLA-B*5701 in this population is similar to the one found in pure Caucasians.


Molecular Analyses of Early-Onset Gastric Cancer in Brazilian Patients: TP53 Mutations, Cadherin-Catenin and Mucins Proteins Expression  [PDF]
Edaise Maria da Silva, José Humberto Tavares Guerreiro Fregnani, Ghyslaine Martel, Wilson Luiz Costa Jr., Felipe José Fernández Coimbra, Maria Isabel Waddington Achatz, Pierre Hainaut, Fernando Augusto Soares
Journal of Cancer Therapy (JCT) , 2013, DOI: 10.4236/jct.2013.41A005
Abstract:

Early gastric carcinomas may develop with a molecular profile differing from sporadic carcinomas occurring at a later age. In this study, we analyzed a retrospective series of 88 patients with gastric adenocarcinoma diagnosed before the age of 45 years for the presence of TP53 mutations, clinicopathological features and immunohistochemistry to evaluate the expression of markers considered to be important in gastric carcinogenesis (E-cadherin, β-catenin, MUC1, MUC2, MUC5AC, MUC6 and p53). The majority of proportion of tumors were diffuse-type (70%) and advanced stage (56%). Familial history of cancer was positive in 21% of the cases. There was a significant association between altered expression of E-cadherin and β-catenin, and between p53 expression and perineural invasion. TP53 mutations were detected in 14.5% of evaluated cases, including a germline mutation (p.R337H) in a 12-year old patient. Overall survival analysis showed significant differences in relation with tumor stage and histopathology. The evaluated biomarkers did not present prognostic value in non-exploratory multivariate analyses. The low frequency of TP53 mutations in this series suggests these alterations are not a major molecular event in gastric cancer occurring at early age, although the identification of a case with germline p.R337H mutation is consistent with the hypothesis that a small proportion of early, apparently sporadic gastric cancer, may be associated with widespread Brazilian founder mutations. Further studies are needed to evaluate the prognostic significance of markers for specific groups of patients according to tumor histology and familial history.

Tratamento da doen?a hemorroidária com ligadura elástica: estudo prospectivo com 59 pacientes
Motta, Marcel Machado da;Silva Júnior, José Batista da;Santana, Larice Oliveira;Ferdandes, Igor Lima;Moura, Alex Rodrigues;Prudente, Ana Carolina Lisboa;Torres Neto, Juvenal da Rocha;
Revista Brasileira de Coloproctologia , 2011, DOI: 10.1590/S0101-98802011000200005
Abstract: rubber band ligation (rb) is considered a minimally invasive method for the treatment of hemorrhoidal disease (hd) and has advantages in relation to hemorrhoidectomy whereby: simplicity of execution, outpatient realization and no need of anesthesia. it is an effective method, especially in the hd grade ii. however, shows complications, and the most frequents are: anal pain, tenesmus, hematochezia and urinary retention. some studies have shown severe complications as major bleeding that needs blood transfusion. thus, this study evaluated the effectiveness and the morbidity of the treatment of hd by the method of rb. it was a prospective study with 59 patients. five (8,5%) patients had hd graded in the first degree, 33 (55,9%) in the second degree and 21 (35,6%) in the third. all patients were submitted to at least two sessions. in the 135 sessions performed, we found: hematochezia in 62 (45.9%), severe pain in 39 (28.9%), vagal symptoms in 10 (7.4%) and pseudostrangulation in 1 (0.7%) session. the cure rate of hemorrhoidal prolapse among patients with hd grade ii was 87.9% and among those with hd grade iii, 76.2%. the treatment of hd by the method of rb proved to be safe and with good cure rate.
Prevalência da doen?a do refluxo gastroesofágico em pacientes com fibrose pulmonar idiopática
Bandeira, Cristiane Dupont;Rubin, Adalberto Sperb;Cardoso, Paulo Francisco Guerreiro;Moreira, José da Silva;Machado, Mirna da Mota;
Jornal Brasileiro de Pneumologia , 2009, DOI: 10.1590/S1806-37132009001200004
Abstract: objective: to determine the prevalence of gastroesophageal reflux disease (gerd) and to evaluate its clinical presentation, as well as the esophageal function profile in patients with idiopathic pulmonary fibrosis (ipf). methods: in this prospective study, 28 consecutive patients with ipf underwent stationary esophageal manometry, 24-h esophageal ph-metry and pulmonary function tests. all patients also completed a symptom and quality of life in gerd questionnaire. results: in the study sample, the prevalence of gerd was 35.7%. the patients were then divided into two groups: gerd+ (abnormal ph-metry; n = 10) and gerd- (normal ph-metry; n = 18). in the gerd+ group, 77.7% of the patients presented at least one typical gerd symptom. the ph-metry results showed that 8 (80%) of the gerd+ group patients had abnormal supine reflux, and that the reflux was exclusively in the supine position in 5 (50%). in the gerd+ and gerd- groups, respectively, 5 (50.0%) and 7 (38.8%) of the patients presented a hypotensive lower esophageal sphincter, 7 (70.0%) and 10 (55.5%), respectively, presenting lower esophageal dysmotility. there were no significant differences between the groups regarding demographic characteristics, pulmonary function, clinical presentation or manometric findings. conclusions: the prevalence of gerd in the patients with ipf was high. however, the clinical and functional characteristics did not differ between the patients with gerd and those without.
Bronquiectasias: aspectos diagnósticos e terapêuticos Estudo de 170 pacientes
Moreira, José da Silva;Porto, Nelson da Silva;Camargo, José de Jesus Peixoto;Felicetti, José Carlos;Cardoso, Paulo Francisco Guerreiro;Moreira, Ana Luiza Schneider;Andrade, Cristiano Feijó;
Jornal de Pneumologia , 2003, DOI: 10.1590/S0102-35862003000500003
Abstract: background: bronchiectasis is a frequently found disease in medical practice in brazil leading to significant morbidity and decrease in quality of life of the affected individuals. objectives: to study diagnostic and therapeutic aspects in a series of hospitalized patients with bronchiectasis in a department of pulmonary diseases. method: signs, symptoms, microbiological and radiographic data, and therapeutic results were studied in 170 hospitalized patients between 1978 and 2001 - females 62.4%, males 37.6%, and aged from 12 to 88 years (mean age 36.8 yrs). previous history of pneumonia in childhood was detected in 52.5% of the patients, tuberculosis in 19.8%; 8.8% had bronchial asthma, and 2 had kartagener's syndrome. results: the most common symptoms were cough (100.0%), expectoration (96.0%) and pulmonary rales (66.0%). the pulmonary lesions were unilateral in 46.5% of the cases. pneumococcus, h. influenzae or mixed flora were found in 85.0% of the examined sputa. all 170 patients received antibiotics and postural drainage, and 88 of them (younger and with a higher functional reserve) were also submitted to pulmonary resections (82 unilateral and 6 bilateral). two deaths occurred, and repetitive hospitalizations were more frequent among the clinically treated patients. the follow up showed that most of the surgically treated patients had significant symptoms improvement and rarely needed to be re-hospitalized. conclusions: in the majority of the patients, lung resection surgery improved permanently the prolonged bronchopulmonary symptoms of patients with bronchiectasis, differently from the patients who received only clinical treatment.
Anus neoplasm: study of a case series
Fernandes, Igor Lima;Santana, Larice Oliveira;Silva Júnior, José Batista Da;Motta, Marcel Machado Da;Moura, Alex Rodrigues;Prudente, Ana Carolina Lisb?a;Torres Neto, Juvenal Da Rocha;
Journal of Coloproctology (Rio de Janeiro) , 2011, DOI: 10.1590/S2237-93632011000300009
Abstract: anus neoplasm accounts for 2 to 4% of colorectal tumors, being more prevalent around the seventh and the eighth decades. females are mostly affected, and the ratio is 3:1. its increased prevalence amongst the population in the past years is probably related to the higher number of people that are affected by sexually transmitted diseases, mainly human papillomavirus (types 16 and 18, mostly) and/or the human immunodeficiency virus. diagnosis is based on clinical findings and anatomopathological tests. the treatment of choice is radiochemotherapy, and the rescue surgery with abdominoperineal resection is used for recurrence and persistence cases. a retrospective and prospective longitudinal observational study was performed with 11 patients diagnosed with anal neoplasm from 2004 to 2010. six (54.5%) were females and five (45.5%) were males. the incidence was higher in the sixth decade, at the mean age of 54.45 years. the most frequent histological type observed was the epidermoid carcinoma, and the most frequent cell differentiation type was the moderately differentiated. chemotharapy associated with radiotherapy was used in 81.9% of the patients, and abdominoperineal resection was necessary as a rescue surgery in 18.2% of the patients.
In vivo microbiological evaluation of the effect of biomechanical preparation of root canals using different irrigating solutions
Tanomaru Filho, Mário;Yamashita, José Carlos;Leonardo, Mario Roberto;Silva, Léa Assed Bezerra da;Tanomaru, Juliane Maria Guerreiro;Ito, Izabel Yoko;
Journal of Applied Oral Science , 2006, DOI: 10.1590/S1678-77572006000200008
Abstract: the aim of this study was to evaluate the antimicrobial effect of biomechanical preparation using different irrigating solutions. seventy-eight root canals from premolars of four dogs were used. after experimental induction of periapical lesions, the root canals were prepared using the following solutions for irrigation: group 1) 2.5% sodium hypochlorite (naocl); group 2) 2% chlorhexidine (chx); group 3) saline solution and group 4) control group with no biomechanical preparation. the microbiological evaluation of the root canals was performed by counting the colony forming units (cfus) using different culture mediums. two absorbent paper cones were used in each root canal in order to collect the microbiological samples before, and thirty days after the biomechanical preparation. the culture plates were incubated in aerobic, anaerobic and microaerophilic environment. statistical evaluation was carried out using analysis of variance, tukey and student tests. the results demonstrated that there was reduction in the number of microorganisms in the naocl and chx groups (p<0.05). there was greater effectiveness in the chlorhexidine group. the group that used saline solution and the control group presented an increased number of microorganisms. it can be concluded that the use of antimicrobial irrigating solutions during biomechanical preparation promotes the reduction of endodontic microbiota. however, a considerable number of microorganisms were still observed.
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