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Search Results: 1 - 10 of 228583 matches for " JORGE Gracinda L. "
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Estudo da libera??o renal de glicose em coelhos submetidos a hepatectomia total funcional e infus?o de noradrenalina
FRANCHI-TEIXEIRA, Antonio Roberto;JORGE, Gracinda L.;LEONARDI, Luís Sérgio;SAAD, Mário José Abdalla;
Arquivos de Gastroenterologia , 2001, DOI: 10.1590/S0004-28032001000300008
Abstract: aim ? to study the possible endogenous sources of glucose in the absence of the liver (equivalent to the anhepatic period of liver transplantation). material and methods - a experimental model of total functional hepatectomy in anesthetised rabbits was developed. the aorta and the right renal vein were catheterised in order to collect blood samples to measure glucose contents. the animals were divided into two groups: group 1, 5 animals underwent only norepinephrine infusion; group 2, 15 animals underwent norepinephrine infusion and submitted to total functional hepatectomy. results - in group 2, before the hepatectomy, arterial glucose levels were higher than venous ones and after the liver removal, the venous levels became higher than the arterial ones. this pattern showed an inversion in the glicemic curves. in group 1 this pattern was not observed. conclusion - the glicemic curves behavior observed in group 2 is not due to norepinephrine infusion, but represents renal glucose release after total functional hepatectomy.
Estudo da libera o renal de glicose em coelhos submetidos a hepatectomia total funcional e infus o de noradrenalina
FRANCHI-TEIXEIRA Antonio Roberto,JORGE Gracinda L.,LEONARDI Luís Sérgio,SAAD Mário José Abdalla
Arquivos de Gastroenterologia , 2001,
Abstract: Objetivo - Estudar as prováveis fontes endógenas de glicose na ausência do fígado (equivalente à fase anepática do transplante de fígado). Material e Métodos - Criou-se um modelo experimental de hepatectomia total funcional em coelhos anestesiados. A aorta e a veia renal direita foram cateterizadas para colheita de glicemias seriadas. Os animais foram divididos em dois grupos: grupo 1, com 5 animais submetidos apenas a infus o de noradrenalina e grupo 2, constando de 15 animais também submetidos a infus o de noradrenalina e tiveram seus fígados retirados. Resultados - Demonstram que, no grupo 2, antes da hepatectomia, as dosagens de glicemias arteriais foram maiores que as venosas, e após a retirada do órg o, estes valores se tornaram menores. Isto configurou invers o das curvas das glicemias. No grupo 1 n o foi observada essa invers o. Conclus o - Conclui-se que esse comportamento das curvas de glicemia observadas nos animais do grupo 2 n o se deve à a o da noradrenalina e significa libera o renal de glicose após a hepatectomia.
A Method to Estimate Spatial Resolution in 2-D Seismic Surface Wave Tomographic Problems  [PDF]
Jorge L. de Souza
International Journal of Geosciences (IJG) , 2014, DOI: 10.4236/ijg.2014.58068
Abstract:

A novel methodology to quantify the spatial resolution in 2-D seismic surface wave tomographic problems is proposed in this study. It is based on both the resolving kernels computed via full resolution matrix and the concept of Full Width at Half Maximum (FWHM) of a Gaussian function. This method allows estimating quantitatively the spatial resolution at any cell of a gridded area. It was applied in the northeastern Brazil and the estimated spatial resolution range is in agreement with all previous surface wave investigations in the South America continent.

A new method for the experimental induction of secundary biliary cirrhosis in wistar rats
Jorge Gracinda De Lourdes,Leonardi Luiz Sergio,Boin Ilka de Fatima Santana Ferreira,Silva Jr Orlando de Castro e
Acta Cirurgica Brasileira , 2001,
Abstract: The aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (SBF). Forty-seven Wistar rats were submitted to hepatic duct obstruction (OB group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. This technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. Nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. Blood samples were collected for the measurement of total bilirubin (TB), alkaline phosphatase (AP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver fragments were removed for morphological study. Thirty days after surgery TB, AP, ALT and AST levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of SBF in the OB group was confirmed by morphological study of the liver. There was technical failure in 31.92% cases. The survival was 100% at fifteen days and 82.97% at the end of the experiment. We concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. This technique can be carried out from cholestasis to fibrosis.
Quantification of natural populations of Gluconacetobacter diazotrophicus and Herbaspirillum spp. In sugar cane (Saccharum spp.) Using differente polyclonal antibodies
Silva-Froufe, Lúcia Gracinda da;Boddey, Robert Michael;Reis, Veronica Massena;
Brazilian Journal of Microbiology , 2009, DOI: 10.1590/S1517-83822009000400018
Abstract: the species gluconacetobacterdiazotrophicus, herbaspirillum seropedicae and h. rubrisubalbicans are endophytic n2-fixing [diazotrophic] bacteria which colonise not only roots, but also the aerial tissue of sugar cane. however, the technique most commonly used to quantify the populations of these microbes in plants is by culturing serial dilutions of macerates of plant tissues in n free semi-solid media which are only semi-selective for the species/genera [the most probable number (mpn) technique] and each culture must be further subjected to several tests to identify the isolates at the species level. the use of species-specific polyclonal antibodies with the indirect elisa (enzyme-linked immunosorbent assay) can be an alternative which is rapid and specific to quantify these populations of bacteria. this study was performed to investigate the viability of adapting the indirect elisa technique to quantify individually the populations of these three species of diazotroph within the root and shoot tissues of sugarcane. the results showed that species-specific polyclonal antibodies could be obtained by purifying sera in protein-a columns which removed non-specific immuno-globulins. it was possible to quantify the three bacterial species in the brazilian sugarcane variety sp 70-1143 in numbers above 105 cells per g fresh weight in roots, rhizomes and leaves. the numbers of the different bacterial species evaluated using the elisa technique were found to be higher than when the same populations were evaluated using the mpn technique, reaching 1400 times greater for g. diazotrophicus and 225 times greater for herbaspirillum spp. these results constitute the first quantification of herbaspirillum using immunological techniques.
Herbaspirillum seropedicae and sugarcane endophytic interaction investigated by using high pressure freezing electron microscopy
Silva, Lúcia Gracinda da;Miguens, Flávio Costa;Olivares, Fabio Lopes;
Brazilian Journal of Microbiology , 2003, DOI: 10.1590/S1517-83822003000500023
Abstract: the interaction between sugar cane plantlets and h. seropedicae was investigated using high pressure freezing followed by freeze substitution. microscopical observation showed consistent differences between this approaches when compared with the conventional preparation, specially related to appearance of the bacteria cell and the endophytic attachment to the host cell wall.
Promo??o de enraizamento de microtoletes de cana-de-a?úcar pelo uso conjunto de substancias húmicas e bactérias diazotróficas endofíticas
Marques Júnior, Roberto Batista;Canellas, Luciano Pasqualoto;Silva, Lúcia Gracinda da;Olivares, Fábio Lopes;
Revista Brasileira de Ciência do Solo , 2008, DOI: 10.1590/S0100-06832008000300020
Abstract: besides the direct nutritional effect of mineralization of organic matter or by biological nitrogen fixation activity, the humic substances and endophytic diazotrophic bacteria can directly influence plant metabolism, modifying plant growth and development patterns. the purpose of this study was to evaluate the effect of the combined use of humic acid (ha) and herbaspirillum seropedicae, an endophytic nitrogen-fixing bacteria, on the root growth of seed pieces - heat-treated or not - of the sugarcane variety rb 72 454. after hot water treatment, the cane cuttings were immersed for 12 hours as follows: in water (control plant), in ha solution (20 mg l-1 of c) from vermicompost, in bacterial inoculant of h. seropedicae, strain hrc54 (108 cells ml-1), and in a combination of bacteria and ha. root growth was improved by 60 to 118 % in length and from 33 % to 233 % in surface area on sugarcane plant treatments compared to control, with more pronounced effect in plants under heat treatment. likewise, the positive effect of the combinded treatment (bacteria inoculation and humic acid) on shoot and root biomass was significant compared to the control with heat-treated cuts. for non-heated seed pieces, bacteria inoculation did not result in a positive plant growth effect, but only in the presence of humic acid. the combined or isolated use of both ha and bacteria did not significantly modify the bacteria population in the root tissue of heat-untreated sugarcane. for heat-treated cuts, bacteria inoculation, combined or not with ha, increased the size of diazotrophic bacteria population on roots. the results highlight the importance of thermotherapy to increase the positive effect of the selected bacteria strain of h. seropedicae. in the case of ha application, plant growth stimulation was not related to thermotherapy. the plant root growth promoting effect induced by both ha and the inoculation of selected nitrogen-fixing bacteria strain could represent an innovative approach
Endotracheal and upper airways suctioning: changes in newborns? physiological parameters
Barbosa, Andréa Lopes;Cardoso, Maria Vera Lúcia Moreira Leit?o;Brasil, Thays Bezerra;Scochi, Carmen Gracinda Silvan;
Revista Latino-Americana de Enfermagem , 2011, DOI: 10.1590/S0104-11692011000600013
Abstract: this study investigated which physiological parameters change when endotracheal and upper airway suctioning is performed immediately before, immediately after and five minutes after this procedure is performed in newborns hospitalized in a neonatal intensive care unit (nicu). this is a quantitative and longitudinal study, before and after type, performed in the nicu of a public institution in the city of fortaleza, ce, brazil. the sample was composed of 104 newborns using oxigenotherapy and who needed endotracheal and upper airway suctioning. the results showed significant alterations in respiratory and heart rates (p<0.05) in neonates using oxyhood and nasal cpap while the pulse significantly changed (p<0.05) in newborns placed in oxyhood, using nasal cpap and mechanical ventilation; oxygen saturation was the only parameter that did not alter significantly. we propose that nurses develop non-pharmacological interventions to reduce potential alterations caused in newborns’ physiological parameters due to this procedure.
Incentivando o vínculo m e-filho em situa o de prematuridade: as interven es de enfermagem no Hospital das Clínicas de Ribeir o Preto
Scochi Carmen Gracinda Silvan,Kokuday Maria de Lourdes do Patrocínio,Riul Maria José Sartori,Rossanez Léa Silvia Sian
Revista Latino-Americana de Enfermagem , 2003,
Abstract: O objetivo deste estudo é descrever as a es da enfermagem realizadas nas unidades neonatais de risco de um hospital-escola de Ribeir o Preto-USP, no sentido de favorecer o vínculo e apego m e-filho em situa o de prematuridade. A enfermeira acompanha os pais na primeira visita, procurando apoiá-los e informando-os sobre os equipamentos que cercam o recém-nascido, incentivando o contato pele-a-pele, o toque e a fala. O acesso e permanência dos pais junto aos bebês de risco s o liberados.Foi implantado programa de visitas dos avós e irm os do prematuro aos bebês, mesmo quando em cuidado intensivo, incentivando o contato familiar. Os pais participam de um grupo de apoio, juntamente com outros pais que passam pela experiência de terem seus filhos prematuros em estado grave e hospitalizados. Consideramos que a nossa experiência tem favorecido o estabelecimento do vínculo e apego m e-filho e família, observando-se maior intera o da família com o bebê, em especial da m e, e maior interesse no aprendizado de seus cuidados, além da satisfa o manifestada pela assistência recebida.
A new method for the experimental induction of secundary biliary cirrhosis in wistar rats
Jorge, Gracinda De Lourdes;Leonardi, Luiz Sergio;Boin, Ilka de Fatima Santana Ferreira;Silva Jr, Orlando de Castro e;Escanhoela, Cecilia Amelia Fazzio;
Acta Cirurgica Brasileira , 2001, DOI: 10.1590/S0102-86502001000200003
Abstract: the aim of this study was to describe a method for the induction of experimental secondary biliary fibrosis (sbf). forty-seven wistar rats were submitted to hepatic duct obstruction (ob group) for thirty days without ligature, section or cannulization causing interruption of biliary flow. this technique was carried out by simple traction of the bile duct passing it through the xiphoid appendix. nine rats were submitted to a sham operation for bile duct stricture and seven rats comprised the control group. blood samples were collected for the measurement of total bilirubin (tb), alkaline phosphatase (ap), alanine aminotransferase (alt) and aspartate aminotransferase (ast). liver fragments were removed for morphological study. thirty days after surgery tb, ap, alt and ast levels were significantly increased in the hepatic duct ligation group compared to the sham operated group and the presence of sbf in the ob group was confirmed by morphological study of the liver. there was technical failure in 31.92% cases. the survival was 100% at fifteen days and 82.97% at the end of the experiment. we concluded that this simple surgical technique may be used to study the consequence of bile duct obstruction which could be a reversible process depending on the obstruction time. this technique can be carried out from cholestasis to fibrosis.
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