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Search Results: 1 - 10 of 297459 matches for " J. Sanitchon "
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Pretreatment with Spermidine Reverses Inhibitory Effects of Salt Stress in Two Rice (Oryza sativa L.) Cultivars Differing in Salinity Tolerance
P. Saleethong,J. Sanitchon,K. Kong-ngern,P. Theerakulpisut
Asian Journal of Plant Sciences , 2011,
Abstract: Environmental stresses including soil salinity are severely affecting rice growth and productivity. The triamine Spermidine (Spd), a member of Polyamines (PAs), a group of phytohormone-like natural amine compounds has been shown to play essential roles in salt stress tolerance in many important crop plants. The effects of exogenously supplied Spd were investigated in two rice cultivars differing in salt tolerance level to determine the roles of Spd on the modulation of physiological parameters related to salt-stress responses. Thirty day-old seedlings of two rice cultivars, Pokkali (salt-tolerant) and KDML 105 (salt-sensitive), grown in nutrient solution were exposed or not to 1 mM Spd for 24 h before submitted to salinized solution containing 150 mM NaCl for 7 days. Salinity stress resulted in significant reduction in plant height, fresh and dry weight of both cultivars but Spd pretreatment significantly increased these growth parameters only in Pokkali. Salt-stress induced considerable disturbance in several physiological processes inhibitory for growth including chlorophyll loss, accumulation of hydrogen peroxide and increase in lipid peroxidation, increased electrolyte leakage and increase in Na+/decrease in K+/Na+. Spd pretreatment led to the reversal of those inhibitory effects in both cultivars. This study showed that exogenous Spd can be applied as short-term pretreatment prior to introduction of salt stress to help elevate salt tolerance of rice and confirmed earlier observations that exogenous Spd offered protective roles on salinity-stressed rice by stabilizing membrane, scavenging free radicals and maintaining K+/Na+ status.
Variation of Microsatellite Markers in a Collection of Lao`s Black Glutinous Rice (Oryza sativa L.)
C. Bounphanousay,P. Jaisil,K.L. McNally,J. Sanitchon
Asian Journal of Plant Sciences , 2008,
Abstract: The genetic diversity of 74 genotypes, including Black Glutinous Rice (BGR) from Lao`s germplasm was assessed using 24 microsatellite markers. A total of 75 alleles were detected at the 24 microsatellite markers. The number of alleles per marker varied from 2 to 7 with an average of 3.1 alleles per locus. The Gene Diversity (GD) and Polymorphism Information Content (PIC) ranged from 0.18 to 0.79 and 0.17-0.76, respectively and the Allele Frequency (AF) ranged from 0.36 to 0.90. The markers were able to classify rice genotypes into four groups; indica rices were put in the three groups while the other group consisted of tropical japonica rice. The first indica group (G1) included 24 genotypes of BGR and five genotypes of white rice. Most of genotypes in this group have thick culms, broad leaf blades, large and bold grain shapes and some of them have purple coloration on all vegetative parts. In other varieties with black pericarp, all other plant parts are green. Indica group 3 (G3) included 25 genotypes of BGR. The special characters of this subgroup were small and slender culms, narrow short leaves, purple leaf margins, purple leaf tips and purple stripes on leaf blades and sheathes. However, the markers used could not differentiate between LG 8215 and LG 7937. Indica group four (G4) consisted of eight genotypes of white rice and the four check varieties. Group (G2) consisted of five white rices, four BGRs and three check varieties.
Effects of Pruning Levels and Fertilizer Rates on Yield of Physic Nut (Jatropha curcas L.)
B. Suriharn,J. Sanitchon,P. Songsri,T. Kesmala
Asian Journal of Plant Sciences , 2011,
Abstract: Appropriate canopy management and proper application of fertilizer under different growing conditions and agronomic practices can obtain reliable yield of physic nut (Jatropha curcas L.). The objective of this study was to determine the effects of pruning level in first year and fertilizer rate of combined NPK in the first and second years on growth and yield of three-year old physic. A split plot design with four replications was used. Three pruning levels of 50, 75 and 90 cm from the ground were assigned in main plots and combinations of NPK fertilizers at the rates of 0, 312.5 and 625 kg ha-1 were arranged randomly in subplots. All pruning levels from the ground did not have significant effects on branch number and branch length (cm), whereas application of fertilizer did increase branch number and branch length especially at the rate of 312.5 kg ha-1. Harvest in the second year gave higher yield (1,559 kg ha-1) than did in the first year (1,180 kg ha-1) for all treatments, suggesting that it is not necessary to prune physic nut every year in commercial plantations. It is recommended to prune the three-year old physic nut at 70 cm from the ground. Pruning at 90 cm from the ground is possible but not at 50 cm. it is also recommended to apply fertilizer to the three-year old physic nut under rainfed conditions at the rate not exceeding 312.5 kg ha-1 and this should be based on soil analysis. Application of higher rates depressed yield.
Heritability, Phenotypic and Genotypic Correlation of Peanut bud necrosis virus Resistance and Agronomic Traits in Peanut
R. Puttha,S. Jogloy,S. Wongkaew,J. Sanitchon
Asian Journal of Plant Sciences , 2008,
Abstract: The objectives of this study were to estimate heritability for PBNV resistance parameters and to determine phenotypic and genotypic correlations among PBNV resistance parameters and agronomic traits. One hundred and ninety two progenies in the F5 and F6 generations of peanut as well as their parents were evaluated under natural infection of PBNV in a randomized complete block design with six replications for two years. Additional experiment of the duplicated materials was also conducted for agronomic evaluation for one year using similar experimental procedures under conditions that favored optimum agronomic performance. Heritability estimates were low to relatively high for both disease incidence and disease severity, depending on crosses, ranging form 0.10 to 0.90 and 0.00 to 0.79, respectively. Phenotypic and genotypic correlation coefficients for PBND incidence and PBND severity were high, ranging from 0.94** to 0.98** and 0.99** and 1.00**, respectively. Phenotypic and genotypic correlation coefficients between PBNV resistance parameters and agronomic characters were generally low for most pairs of characters, except between PBNV resistance parameters and 100-seed weight for which it was moderate (0.42** to 0.60**) PBNV susceptibility is somewhat associated with larger seed and might hinder the progress of breeding for large-seeded peanut with resistance to PBNV.
Inheritance of Traits Related to Biological Nitrogen Fixation and Genotypic Correlation of Traits Related to Nitrogen Fixation, Yield and Drought Tolerance in Peanut (Arachis hypogaea L.) Under Early Drought
H. Wunna,S. Jogloy,B. Toomsan,J. Sanitchon
Asian Journal of Plant Sciences , 2009,
Abstract: The improvement of peanut for drought tolerance and high N2-fixation is the best way to enhance peanut production under drought condition. Besides, the heritability estimates of traits related N2-fixation and its genetic correlation with yield and drought tolerant traits are useful to formulate the effective breeding program under drought. Therefore, the aims of this study were to estimate the heritabilities (h2) and genotypic correlation (rG) among traits related to N2-fixation (TNf), yield and drought tolerant traits under early drought and non stressed condition. Ninety lines in the F4:8 generations from four peanut crosses were tested under Field Capacity (FC) and one-third Available Water (1/3 AW). Data were recorded for Nodules Dry Weight (NDW), Biomass Production (BM), Pods Yield (PY), number of pod plant-1, number of seed pod-1 and 100 seed weight at harvest. Specific Leaf Area (SLA), SPAD Chlorophyll Meter Reading (SCMR), Harvest Index (HI) and Drought Tolerance Index (DTI) of PY and BM were measured and calculated as drought tolerant traits. The h2 for BM, PY, number of pod plant-1 and 100 seed weight were high for all tested crosses under both water regimes. With exception of HI trait, high h2 estimates, also, were found for drought tolerant traits under both water regimes. The genotypic correlation (rG) between NDW and BM was positive highly significant under both 1/3 AW and FC. BM and PY showed high rG, whereas, BM and 100 seed weight showed moderate rG. Moderate rG was found between BM and SCMR 60 DAE under 1/3 AW and FC. Significant correlations between FC and early drought were found for BM indicating that selection of this trait could be done under both water regimes. BM is possible to select and breed for high N2-fixation, PY and possibly, drought tolerance because of high h2 and significant rG with PY and SCMR 60DAE.
Response to Early Drought for Traits Related to Nitrogen Fixation and Their Correlation to Yield and Drought Tolerance Traits in Peanut (Arachis hypogaea L.)
H. Wunna,S. Jogloy,B. Toomsan,J. Sanitchon
Asian Journal of Plant Sciences , 2009,
Abstract: This study was aimed to examine the response and contribution of early drought to traits related to N2-fixation and pod yield and their correlation to drought tolerance. The experiment was conducted at the Field Crop Research Station of Khon Kaen University, Khon Kaen Province, Thailand in the dry season of 2007/08. Eleven peanut genotypes (ICGV 98300, ICGV 98303, ICGV 98305, ICGV 98308, ICGV 98324, KK 60-3, Tainan 9, KKU 72-1, KKU 60, KK 4 and KKU 1) and two soil moisture levels [field capacity (FC) and 1/3 available water (1/3 AW)] were laid out in a split-plot design with four replications. Early drought treatment was given by maintaining 1/3 AW from emergence to 40 days after emergence followed by adequate water supply. The data were recorded for nodule dry weight (NDW) and biomass production (BM) as traits related to N2-fixation (TNf) at harvest. In addition to, the data on pod yield, number of pod plant-1, number of seed pod-1 and seed size (SZ) were also collected at harvest. Specific leaf area (SLA) and SPAD chlorophyll meter reading (SCMR) were measured on 20, 40, 50 and 60 days after emergence (DAE) as drought tolerance traits and harvest index (HI) was calculated after harvest. Early drought did not affect NDW and BM. Major variation was found among peanut genotypes and ICGV 98305 showed higher NDW and pod yield under drought condition. Significant and consistent correlation was found between NDW and BM, (r = 0.82*, p< 0.05) and (r = 80*, p < 0.05) under FC and 1/3 AW, respectively. The correlation between TNf and pod yield and yield component parameters varied under the two water regimes. Under 1/3 AW, the only positive correlation observed was between SZ and BM and it might be the only reason for increase in pod yield in some genotypes. SCMR at 60 DAE was strongly related with TNf under both water regimes. There was not any correlation between SLA and HI with NDW and BM. SCMR at 60 DAE is useful to detect chlorophyll density and N2-fixation under both water regimes because of its high and constant correlation with TNf.
Chemical and Molecular Characterization of Fragrance in Black Glutinous Rice from Lao PDR
C. Bounphanousay,P. Jaisil,J. Sanitchon,M. Fitzgerald
Asian Journal of Plant Sciences , 2008,
Abstract: Fifty three accessions of lowland Black Glutinous Rice (BGR) and three white rice varieties from Lao PDR were evaluated for variations in 2-acetyl-1-pyrroline (2-AP), a main aroma compound present in fragrant rice and a 257 bp genomic fragment associated with a gene encoding 2-AP. The objective of this study was to characterize the variation in aromatic characters of Lao BGR by using chemical and molecular techniques. Thirty six out of the 56 accessions had aromatic properties, containing 2-acetyl-1-pyrroline (2-AP). The concentrations of 2-AP in unpolished samples were about 50% higher than those of the polished samples. The concentrations of 2-AP in BGR accessions were lower than those of white aromatic rice varieties in which 2-AP was highest in Kai Noi Leuang (0.688 ppm). Seventeen accessions and two of white rice (Kai Noi Leuang and TDK5) had a common 355 bp allele corresponding to that found in Calrose, a non-fragrant variety. A 257 bp allele was found in the 36 varieties of BGR and a local aromatic white rice variety, Hom Nang Nouan, sharing a common allele as in the fragrant variety KDML 105. Surprisingly, Kai Noi Leuang, a popular Lao local aromatic rice variety with the highest 2-AP concentration did not possess a 257 bp fragment which has been identified previously as a fragrant allele. This is the first study of the chemical and molecular aromatic properties of lowland BGR varieties of the Lao PDR and the data obtained will be valuable in screening other germplasm and in planning a breeding program in the country geared towards fragrant rices with superior agronomic properties.
Effects of Gamma Radiation on Germination and Growth Characteristics of Physic Nut (Jatropha curcas L.)
P. Songsri,B. Suriharn,J. Sanitchon,S. Srisawangwong
Journal of Biological Sciences , 2011,
Abstract: Narrow genetic base is a main problem hindering the progress of physic nut breeding. The objective of this study was to compare the effects of different levels of gamma radiation on germination percentage, survival percentage and growth of five physic nut genotypes. The seeds were treated with six levels of gamma radiation (0, 200, 400, 600, 800 and 1000 Gy) and the 5x6 treatment combinations were arranged in a randomized complete block design with three replications. Data were recorded for germination percentage, survival percentage at 15 Days After Germination (DAG) (GR50(15)) and survival percentage at 30 DAG (LD50(30)). As the seedlings did not survive at 600, 800 and 1000 Gy, these treatments were deleted from the successive evaluations of plant height, stem diameter and leaf number. Higher doses of gamma radiation resulted in the significant reductions in germination percentage, survival percentage and plant height. The germination percentages were from 4.0% for 1000 Gy to 82.5% for 0 Gy. The seeds treated with gamma radiation higher than 600 Gy resulted in complete mortality. Difference among genotypes was not significant for these characters. The dosages of gamma radiation to obtain LD50 based on survival percentage 30 DAG was 425 Gy and was not dependent on genotypes. The information is useful for gamma radiation induction of mutation breeding for physic nut.
Application of LW7 marker for identification of progenies with male sterility gene in sweet sorghum population
Darika Bunphan,Prasit Jaisil,Jirawat Sanitchon
Crop Breeding and Applied Biotechnology , 2013,
Abstract: The objectives of this study were to verify the use of LW7 marker in identifying maintainer lines (B-lines) and restorer lines (R-lines) in grain sorghum and sweet sorghum, and to identify B-lines in the F2, BC1F2 and BC2F2 generations. Twenty five accessions of sorghum were evaluated, and LW7 marker correctly identified accessions which presented male sterility gene (rf4) in Suphan Buri1 and 03B cultivars; moreover, these genotypes did not show 779 bp band. The cross between Suphan Buri1 and a male-sterile line (A-line) 03A resulted in a sterilized male, confirming the usefulness of the marker in breeding programs. B-lines in the F2, BC1F2 and BC2F2 generations were identified by LW7 marker. The segregation ratio of 3:1 for male fertility and male sterility in the progenies of the three generations supported the one-gene model of Mendelian segregation. The use of marker assisted selection was successful for line development of sweet sorghum with male sterility
Genetic diversity analysis of rice cultivars (Oryza sativa L.) differing in salinity tolerance based on RAPD and SSR markers
Kanawapee,Nantawan; Sanitchon,Jirawat; Srihaban,Pranee; Theerakulpisut,Piyada;
Electronic Journal of Biotechnology , 2011,
Abstract: thirty rice cultivars were evaluated for salinity tolerance during the seedling stage and were divided into five tolerance groups including tolerant (t), moderately tolerant (mt), moderately susceptible (ms), susceptible (s) and highly susceptible (hs) which comprised 5, 10, 9, 4 and 2 cultivars respectively. genetic diversity of all rice cultivars was evaluated using random amplified polymorphic dna (rapd) and simple sequence repeats (ssr) markers. the cultivars were evaluated for polymorphisms after amplification with 20 random decamer primers and 20 ssr primer pairs. a total of 161 rapd markers and 190 ssr alleles were produced which revealed 68.94% and 89.47% polymorphism respectively. mean genetic similarity coefficient was 0.82 for rapd and 0.70 for ssr. cluster analysis based on rapd markers was effective in grouping cultivars based on their salt tolerance ability. group ia1, ib and iv contained three t, three s and two hs rice cultivars respectively. the mt and ms cultivars which showed similar physiological responses to salinity were resolved into two groups: group ia2 and group ii comprising ten and eight mt/ms cultivars respectively. cluster analysis based on ssr markers separated rice cultivars into groups based on genetic relatedness which did not correspond to salinity tolerance level. the results from this study provided some useful implications for salt tolerance breeding programs. the evaluation of genetic similarity and cluster analysis together with salt tolerance ability provides some useful guides for assisting plant breeders in selecting suitable genetically diverse parents for the crossing program.
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