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Search Results: 1 - 10 of 19651 matches for " Ismael Al-Ramahi "
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Smaug/SAMD4A Restores Translational Activity of CUGBP1 and Suppresses CUG-Induced Myopathy
Maria de Haro,Ismael Al-Ramahi,Karlie R. Jones,Jerrah K. Holth,Lubov T. Timchenko,Juan Botas
PLOS Genetics , 2013, DOI: 10.1371/journal.pgen.1003445
Abstract: We report the identification and characterization of a previously unknown suppressor of myopathy caused by expansion of CUG repeats, the mutation that triggers Myotonic Dystrophy Type 1 (DM1). We screened a collection of genes encoding RNA–binding proteins as candidates to modify DM1 pathogenesis using a well established Drosophila model of the disease. The screen revealed smaug as a powerful modulator of CUG-induced toxicity. Increasing smaug levels prevents muscle wasting and restores muscle function, while reducing its function exacerbates CUG-induced phenotypes. Using human myoblasts, we show physical interactions between human Smaug (SMAUG1/SMAD4A) and CUGBP1. Increased levels of SMAUG1 correct the abnormally high nuclear accumulation of CUGBP1 in myoblasts from DM1 patients. In addition, augmenting SMAUG1 levels leads to a reduction of inactive CUGBP1-eIF2α translational complexes and to a correction of translation of MRG15, a downstream target of CUGBP1. Therefore, Smaug suppresses CUG-mediated muscle wasting at least in part via restoration of translational activity of CUGBP1.
dAtaxin-2 Mediates Expanded Ataxin-1-Induced Neurodegeneration in a Drosophila Model of SCA1
Ismael Al-Ramahi,Alma M Pérez,Janghoo Lim,Minghang Zhang,Rie Sorensen,Maria de Haro,Joana Branco,Stefan M Pulst,Huda Y Zoghbi,Juan Botas
PLOS Genetics , 2007, DOI: 10.1371/journal.pgen.0030234
Abstract: Spinocerebellar ataxias (SCAs) are a genetically heterogeneous group of neurodegenerative disorders sharing atrophy of the cerebellum as a common feature. SCA1 and SCA2 are two ataxias caused by expansion of polyglutamine tracts in Ataxin-1 (ATXN1) and Ataxin-2 (ATXN2), respectively, two proteins that are otherwise unrelated. Here, we use a Drosophila model of SCA1 to unveil molecular mechanisms linking Ataxin-1 with Ataxin-2 during SCA1 pathogenesis. We show that wild-type Drosophila Ataxin-2 (dAtx2) is a major genetic modifier of human expanded Ataxin-1 (Ataxin-1[82Q]) toxicity. Increased dAtx2 levels enhance, and more importantly, decreased dAtx2 levels suppress Ataxin-1[82Q]-induced neurodegeneration, thereby ruling out a pathogenic mechanism by depletion of dAtx2. Although Ataxin-2 is normally cytoplasmic and Ataxin-1 nuclear, we show that both dAtx2 and hAtaxin-2 physically interact with Ataxin-1. Furthermore, we show that expanded Ataxin-1 induces intranuclear accumulation of dAtx2/hAtaxin-2 in both Drosophila and SCA1 postmortem neurons. These observations suggest that nuclear accumulation of Ataxin-2 contributes to expanded Ataxin-1-induced toxicity. We tested this hypothesis engineering dAtx2 transgenes with nuclear localization signal (NLS) and nuclear export signal (NES). We find that NLS-dAtx2, but not NES-dAtx2, mimics the neurodegenerative phenotypes caused by Ataxin-1[82Q], including repression of the proneural factor Senseless. Altogether, these findings reveal a previously unknown functional link between neurodegenerative disorders with common clinical features but different etiology.
A Genome-Scale RNA–Interference Screen Identifies RRAS Signaling as a Pathologic Feature of Huntington's Disease
John P. Miller,Bridget E. Yates,Ismael Al-Ramahi,Ari E. Berman,Mario Sanhueza,Eugene Kim,Maria de Haro,Francesco DeGiacomo,Cameron Torcassi,Jennifer Holcomb,Juliette Gafni,Sean D. Mooney,Juan Botas,Lisa M. Ellerby ,Robert E. Hughes
PLOS Genetics , 2012, DOI: 10.1371/journal.pgen.1003042
Abstract: A genome-scale RNAi screen was performed in a mammalian cell-based assay to identify modifiers of mutant huntingtin toxicity. Ontology analysis of suppressor data identified processes previously implicated in Huntington's disease, including proteolysis, glutamate excitotoxicity, and mitochondrial dysfunction. In addition to established mechanisms, the screen identified multiple components of the RRAS signaling pathway as loss-of-function suppressors of mutant huntingtin toxicity in human and mouse cell models. Loss-of-function in orthologous RRAS pathway members also suppressed motor dysfunction in a Drosophila model of Huntington's disease. Abnormal activation of RRAS and a down-stream effector, RAF1, was observed in cellular models and a mouse model of Huntington's disease. We also observe co-localization of RRAS and mutant huntingtin in cells and in mouse striatum, suggesting that activation of R-Ras may occur through protein interaction. These data indicate that mutant huntingtin exerts a pathogenic effect on this pathway that can be corrected at multiple intervention points including RRAS, FNTA/B, PIN1, and PLK1. Consistent with these results, chemical inhibition of farnesyltransferase can also suppress mutant huntingtin toxicity. These data suggest that pharmacological inhibition of RRAS signaling may confer therapeutic benefit in Huntington's disease.
Attitudes of consumers and healthcare professionals towards the patient package inserts - a study in Palestine
Al-Ramahi R,Zaid AN,Kettana N,Sweileh W
Pharmacy Practice (Granada) , 2012,
Abstract: Reading the patient package inserts (PPIs) is a key source of information about medications for patients. They should be clear and understandable to the general population. Objectives: The aims of this study were to obtain base-line data on the extent of reading PPIs by consumers and possible factors that might affect this; to explore the attitude of the Palestinian public and healthcare professionals towards the patient package inserts (PPIs); and to review a random sample of PPIs for the availability of different information.Methods: The first part of the study was a cross-sectional self-administered questionnaire. The questionnaire for consumers included 15 items. The questionnaire for healthcare professionals included 10 items and it was very similar to that of consumers with some modifications. In the second part, a random sample of PPIs was reviewed. In our community pharmacies, where medications are arranged according to their producing company, a researcher was asked to choose randomly 10-15 medications for every company to check for the availability of pharmacological, pharmaceutical and clinical information. Results: A total of 304 healthcare professionals out of 320 (95.0%) and 223 consumers out of 240 (92.9%) accepted to answer the survey. Forty five percent consumers reported that they always read the PPIs, and 29.3% said that they read the PPIs most of the times. Increased rate of reading the leaflet was found among females (P = 0.047). The preferred language for the PPIs was Arabic for most of the consumers (89.6%) while it was English for most of the healthcare professionals (80.8%). 35.9% of the consumers and 43.6% of the healthcare professionals found the font size suitable. 42.3% of the consumers and 25.5% of the healthcare professionals said that they found the information in the PPIs useful and enough. The PPIs of 135 randomly sampled medications were reviewed. Many important sections were not found in the PPIs' sample. Conclusion: A high percentage of consumers read the PPIs, but still this needs to be improved. People would appreciate a more detailed and clear PPI. Pharmacists should advocate reading the PPIs but they need to provide consumers with detailed counseling to compensate for the missing information in some of the PPIs. Authorities and manufacturers should implement appropriate measures to regulate the quality and quantity of information in the PPIs.
Attitudes of consumers and healthcare professionals towards the patient package inserts-a study in Palestine
Al-Ramahi,Rowa′; Zaid,Abdel N.; Kettana,Na'em; Sweileh,Waleed; Al-Jabi,Doa';
Pharmacy Practice (Internet) , 2012, DOI: 10.4321/S1886-36552012000100010
Abstract: reading the patient package inserts (ppis) is a key source of information about medications for patients. they should be clear and understandable to the general population. objectives: the aims of this study were to obtain base-line data on the extent of reading ppis by consumers and possible factors that might affect this; to explore the attitude of the palestinian public and healthcare professionals towards the patient package inserts (ppis); and to review a random sample of ppis for the availability of different information. methods: the first part of the study was a cross-sectional self-administered questionnaire. the questionnaire for consumers included 15 items. the questionnaire for healthcare professionals included 10 items and it was very similar to that of consumers with some modifications. in the second part, a random sample of ppis was reviewed. in our community pharmacies, where medications are arranged according to their producing company, a researcher was asked to choose randomly 10-15 medications for every company to check for the availability of pharmacological, pharmaceutical and clinical information. results: a total of 304 healthcare professionals out of 320 (95.0%) and 223 consumers out of 240 (92.9%) accepted to answer the survey. forty five percent consumers reported that they always read the ppis, and 29.3% said that they read the ppis most of the times. increased rate of reading the leaflet was found among females (p = 0.047). the preferred language for the ppis was arabic for most of the consumers (89.6%) while it was english for most of the healthcare professionals (80.8%). 35.9% of the consumers and 43.6% of the healthcare professionals found the font size suitable. 42.3% of the consumers and 25.5% of the healthcare professionals said that they found the information in the ppis useful and enough. the ppis of 135 randomly sampled medications were reviewed. many important sections were not found in the ppis' sample. conclusion: a high perce
Novel Techniques for Face Recognition Identification and Labeling
Mikhled Alfaouri,Hilal M. Al-Bayatti,Nada N. Al-Ramahi
International Journal of Soft Computing , 2012,
Abstract: In this study the method is presented for face image recognition identification and labeling. The method is based on using the combination of the Discrete Multi-Wavelet Transform (DMWT) and the Inverse Discrete Wavelet Transform (IDWT) followed by a Neural Network (NN). In this method the resulting coefficients were computed by the proposed multi-wavelets transform for single-level decomposition. It can be readily observed that the lowpass block (upper left corner) actually contains one lowpass subband and three bandpass subbands. The LL subbands resemble a smaller version of the original image. In this paper the Inverse Discrete Wavelet Transform (IDWT) of LL coefficients will be obtained. The resultant of feature extraction is obtained by the Inverse Discrete Wavelet Transform, (IDWT) and applied to the first column to the Neural Network (NN) for recognition identification of the face image. This study gave an excellent result: 99% for a database of 50 different face images were excellent which indicates that the suggested algorithm in an excellent tool to process the database of standard pose of face image. A detection rate of 90% was achieved for facial movement such as a smile or other moves of face identification. The algorithm is implemented using MATLAB programming languages version 7.
Novel Techniques for Face Recognition Identification and Labeling
Mikhled Alfaouri,Hilal M. Al-Bayatti,Nada N. Al-Ramahi
International Journal of Soft Computing , 2012,
Abstract: In this study the method is presented for face image recognition identification and labeling. The method is based on using the combination of the Discrete Multi-Wavelet Transform (DMWT) and the Inverse Discrete Wavelet Transform (IDWT) followed by a Neural Network (NN). In this method the resulting coefficients were computed by the proposed multi-wavelets transform for single-level decomposition. It can be readily observed that the lowpass block (upper left corner) actually contains one lowpass subband and three bandpass subbands. The LL subbands resemble a smaller version of the original image. In this study the Inverse Discrete Wavelet Transform (IDWT) of LL coefficients will be obtained. The resultant of feature extraction is obtained by the Inverse Discrete Wavelet Transform, (IDWT) and applied to the first column to the Neural Network (NN) for recognition identification of the face image. This method gave an excellent result: 99% for a database of 50 different face images were excellent which indicates that the suggested algorithm in an excellent tool to process the database of standard pose of face image. A detection rate of 90% was achieved for facial movement such as a smile or other moves of face identification. The algorithm is implemented using MATLAB programming languages version 7.
Estabilidade química de dois adesivos dentinários de frasco único em fun o da perda de solvente
Yevenes, Ismael et al.
Revista Odonto Ciência , 2008,
Abstract: Objetivo: Dois sistemas adesivos de frasco único, Single-Bond e Prime and Bond NT, contendo etanol-água e acetona como solventes, respectivamente, foram avaliados quanto à sua estabilidade química devido à perda de solvente. Metodologia: A estabilidade foi medida pelas altera es de peso do adesivo em ensaios de degrada o acelerada sob condi es de temperatura e umidade acima das condi es normais ao longo de 15 dias: 25±2oC / 60±5% RH, 30±2oC / 65±5% RH, 40±2oC / 70±5% RH, e 50±2oC / 75±5% RH. Para cada temperatura, a inclina o e a taxa constante de perda de peso foram registradas para obter o gráfico de Arrhenius. O conteúdo de água no adesivo Single Bond foi determinado por ensaio K Fisher e a quantidade total de solvente foi obtida pela diferen a com o peso total. Resultados: Os resultados para Single Bond indicaram conteúdo de 5,90±0,08% de água e 39,14±1,19%de álcool. Para Prime and Bond NT a porcentagem de acetona foi de 43,08±1,15%. As altera es de peso depois dos ensaios de degrada o acelerada mostraram que a perda de solvente no adesivo Single-Bond foi menor que no adesivo Prime and Bond NT. Conclus o: Os resultados sugerem que a estabilidade química do adesivo contendo etanolágua é maior que da formula o contendo acetona.
Caries and fluorosis in the Santiago metropolitan region in Chile: the impact of the fluoridation of the water = Cárie e fluorose na regi o metropolitana de Santiago, Chile: impacto da fluoreta o da água
Yévenes López, Ismael et al.
Revista Odonto Ciência , 2011,
Abstract: Objetivo: Avaliar o dano e a prevalência de cárie e fluorose em crian as e adolescentes na regi o metropolitana do Chile, após oito anos de fluoreta o da água potável, em compara o com o estudo de base em 1996. Metodologia: Desenho: estudo de prevalência. A amostra probabilística, estratificada por nível socioecon mico, constituiu-se de 2. 323 escolares de 6-8 e 12 anos na Regi o Metropolitana. Todos os alunos foram clinicamente examinados por dentistas calibrados, utilizando critérios de sele o e indicadores propostos pela OMS para determinar cárie e fluorose dentária. Resultados: Cerca de 24% das crian as estavam livres de cáries. A média de CPOD foi de 3,18 em escolares de 6 a 8 anos. O CPOD foi de 0,59 em crian as de 6 a 8 anos e de 2,6 em crian as de 12 anos em rela o ao estudo de base, sendo essas diferen as estatisticamente significativas. Houve diferen as significativas devido ao nível socioecon mico, sendo que os valores médios foram maiores em crian as de baixo nível socioecon mico. Cerca de 14% das crian as apresentaram fluorose dentária, em geral de grau muito leve. Conclus o: Após 8 anos de fluoreta o da água potável na regi o metropolitana, observou-se um aumento de 100% de crian as sem história de cárie e significativa diminui o de danos. A fluorose dentária aumentou, mas em graus mais leves.
Prevalence of dental caries in preschool children in Pe aflor, Santiago, Chile
Yévenes López, Ismael et al.
Revista Odonto Ciência , 2009,
Abstract: Objetivo: Apesar da redu o de prevalência de cárie no Chile, muito pouco se sabe sobre a prevalência da doen a em dentes decíduos. O objetivo deste estudo foi avaliar a prevalência de cárie em crian as de 3 a 5 anos de idade em Pe aflor, uma comunidade semirrural em Santiago, Chile. Metodologia: A amostra foi constituída por um total de 94 crian as de famílias de baixo nível sócio-econ mico. Um cirurgi o dentista previamente treinado e calibrado (kappa 0. 87) realizou os exames clínicos e registrou a porcentagem de crian as sem cáries, severidade da cárie e índice ceo-s, usando a metodologia descrita pela OMS. Resultados: Os resultados mostraram que 50% das crian as de 3 anos n o apresentavam cáries em compara o com 42,2% das crian as de 5 anos de idade. N o houve diferen a estatisticamente significativa entre gênero para a prevalência da doen a. Para o total da amostra, a porcentagem de crian as sem cáries foi de 43,2%. O índice ceo-s na popula o estudada foi 1,30; 2,51 e 2,52, aos 3, 4 e 5 anos de idade, respectivamente. Conclus o: Este estudo mostrou que as crian as da comunidade de Pe aflor n o alcan aram os objetivos propostos pela OMS para o ano 2000. Isto indica que esta popula o necessita da aplica o de estratégias preventivas e restauradoras adicionais.
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