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Search Results: 1 - 10 of 26183 matches for " Il-Chi Chang "
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Correlation of Anaplastic Lymphoma Kinase Overexpression and the EML4-ALK Fusion Gene in Non-Small Cell Lung Cancer by Immunohistochemical Study
Tai-Di Chen,Il-Chi Chang,Hui-Ping Liu,Yi-Cheng Wu
Chang Gung Medical Journal , 2012,
Abstract: Background: Recently the echinoderm microtubule-associated protein-like 4-anaplastic lymphoma kinase (EML4-ALK) fusion gene with transforming activity was identified in non-small cell lung cancer (NSCLC). In addition, NSCLC patients with the EML4-ALK fusion gene had a dramatic response and longer progression free survival after ALK inhibitor treatment than those without this fusion gene. However, the incidence and clinical and molecular characteristics of the EML4-ALK fusion gene in NSCLC patients of Taiwan are still unclear.Methods:Sixty-four fresh frozen tumor specimens were obtained from the tissue bank of Chang Gung Memorial Hospital for RNA extraction and EML4-ALK fusion gene detection. Paraffin sections of lung tumors from all of these patients were available and were analyzed for ALK protein expression by immunohistochemical (IHC) study. The results were correlated with clinical and molecular biomarkers.Results: Three of the 64 tumors (4.7%) had the EML4-ALK fusion gene. Two were adenocarcinomas, and one was adenosquamous carcinoma. Twenty patients with non-squamous cell carcinomas had epidermal growth factor receptor (EGFR) mutations, so the EML4-ALK fusion gene was found in 14.3% of EGFR wild type non-squamous cell carcinomas. Two tumors were variant 3 (3a+3b with 3b predominant) and had strong staining (3+) for ALK by IHC stains. One tumor was variant 1 and had moderate staining (2+) for ALK. None of the ALK wild type tumors had strong staining for ALK. When compared with other clinical and molecular features, only the IHC stain for ALK was significantly correlated with the EML4-ALK fusion gene (p = 0.0002).Conclusions: ALK overexpression detected by IHC study could be a promising detection method for the EML4-ALK fusion gene and is worth further confirmation with more samples.
Comparison of IHC, FISH and RT-PCR Methods for Detection of ALK Rearrangements in 312 Non-Small Cell Lung Cancer Patients in Taiwan
Yi-Cheng Wu, Il-Chi Chang, Chi-Liang Wang, Tai-Di Chen, Ya-Ting Chen, Hui-Ping Liu, Yen Chu, Yu-Ting Chiu, Tzu-Hua Wu, Li-Hui Chou, Yi-Rong Chen, Shiu-Feng Huang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0070839
Abstract: Background Recently Echinoderm microtubule-associated protein-like 4- anaplastic lymphoma kinase (EML4-ALK) fusion gene has become an important biomarker for ALK tyrosine kinase inhibitor (crizotinib) treatment in NSCLC. However, the best detection method and the significance of EML4-ALK variant types remain uncertain. Methods Reverse transcriptase-polymerase chain reaction (RT-PCR), fluorescence in Situ hybridization (FISH) and Immunohistochemical (IHC) stain were performed on tumor tissues of 312 NSCLC patients for detection of ALK rearrangements. Mutation analyses for EGFR and KRAS genes were also performed. Results Thirteen of the 312 patients (4.17%) had ALK rearrangements detected by RT-PCR. If RT-PCR data was used as the gold standard, FISH tests had a low sensitivity (58.33%), but very good specificity (99.32%). IHC stain had better sensitivity (91.67%) than FISH, but lower specificity (79.52%), when the cut off was IHC2+. All of the 8 patients with high abundance of EML4-ALK positive cells in tumor tissues (assessed by the signal intensities of the RT-PCR product), were also have high expression of ALK protein (IHC3+), and positive for FISH, except one failed in FISH. Variants 3a+3b (4/5, 80%) of EML4-ALK fusion gene were more common to have high abundance of EML4-ALK positive cells in tumor tissues than variant 1 (1/3, 33.3%). Meta-analysis of the published data of 2273 NSCLC patients revealed that variant 3 (23/44, 52.3%) was the most common type in Chinese population, while variant 1 (28/37, 75.7%) was most common in Caucasian. Conclusions Among the three detection methods, RT-PCR could detect not only the presence of EML4-ALK fusion gene and their variant types, but also the abundance of EML4-ALK positive cells in NSCLC tumor tissues. The latter two factors might affect the treatment response to anti-ALK inhibitor. Including RT-PCR as a diagnostic test for ALK inhibitor treatment in the prospective clinical trials is recommended.
Identification of Transforming Hepatitis B Virus S Gene Nonsense Mutations Derived from Freely Replicative Viruses in Hepatocellular Carcinoma
Shiu-Feng Huang, Ya-Ting Chen, Wei-Chen Lee, Il-Chi Chang, Yu-Ting Chiu, Yu Chang, Hsiao-Chen Tu, Chiou-Hwa Yuh, Isao Matsuura, Liang-Yu Shih, Ming-Wei Lai, Hong-Dar Isaac Wu, Miin-Fu Chen, Chau-Ting Yeh
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0089753
Abstract: Background & Aims The correlation between chronic hepatitis B virus (HBV) infection and hepatocellular carcinoma (HCC) has been well-established. But the roles of viral factor remain uncertain. Only HBV X gene and nonsense mutations of S gene (C-terminal truncation of HBV surface protein) have been demonstrated to have transforming activity. Whether they play a significant role in hepatocarcinogenesis is still uncertain. Methods Twenty-five HBV-related HCC patients were positive for hepatitis B core antigen (HBcAg) in the cancerous parts of their HCC liver tissues by immunohistochemistry studies, and had available tissue for whole HBV genome sequence analysis. The results were compared with 25 gender and age-matched HBcAg negative HCCs. Plasmids encoding HBV S gene nonsense mutations identified from HBcAg (+) HCC tissue were constructed to investigate their cell proliferation, transformation activity and the oncogenic potentials by xenograft study and in vivo migration assay. Results HBcAg (+) HCC patients were significantly associated with cirrhosis and small tumor size (≦2 cm) when compared with HBcAg (?) HCC patients. Southern blot analyses revealed freely replicative forms of HBV in the cancerous parts of HBcAg(+) HCC. Three nonsense mutations of S gene (sL95*, sW182*, and sL216*) were identified in the HBcAg(+) HCC tumor tissues. sW182* and sL216* were recurrently found in the 25 HBcAg (?) HCC tumor tissue, too. Functional studies of the above 3 non-sense mutations all demonstrated higher cell proliferation activities and transformation abilities than wild type S, especially sW182*. Tumorigenicity analysis by xenograft experiments and in vitro migration assay showed potent oncogenic activity of sW182* mutant. Conclusions This study has demonstrated potent oncogenic activity of nonsense mutations of HBV S gene, suggesting they may play an important role in hepatocarcinogenesis.
Bayesian Decision Analysis for Recurrent Cervical Cancer  [PDF]
Chi-Chang Chang
Open Journal of Clinical Diagnostics (OJCD) , 2014, DOI: 10.4236/ojcd.2014.42013
Abstract: Risk modeling for recurrent cervical cancer requires the development of new concepts and methodologies. Unlike most daily decisions, many medical decision making have substantial consequences, and involve important uncertainties and trade-offs. The uncertainties may be about the accuracy of available diagnostic tests, the natural history of the cervical cancer, the effects of treatment in a patient or the effects of an intervention in a group or population as a whole. With such complex decisions, it can be difficult to comprehend all options “in our heads”. This study applied Bayesian decision analysis to an inferential problem of recurrent cervical cancer in survival analysis. A formulation is considered where individual was expected to experience repeated events, along with concomitant variables. In addition, the sampling distribution of the observations is modelled through a proportional intensity Nonhomogeneous Poisson process. The proposed decision models can provide decision support techniques not only for taking action in the light of all available relevant information, but also for minimizing expected loss. The decision process is useful in selecting the best alternative when a patient with recurrent cervical cancer, in particular, the proposed decision process can provide more realistic solutions.
Juzen-taiho-to (Shi-Quan-Da-Bu-Tang): Scientific Evaluation and Clinical Application
Il-Moo Chang
Evidence-Based Complementary and Alternative Medicine , 2006, DOI: 10.1093/ecam/nel005
Initiative for Developing Evidence-based Standardization of Traditional Chinese Medical Therapy in the Western Pacific Region of the World Health Organization
Il-Moo Chang
Evidence-Based Complementary and Alternative Medicine , 2004, DOI: 10.1093/ecam/neh052
Chi-Cheng Chang
Academic Research International , 2013,
Abstract: It is common to adopt group discussions when determining whether a curriculum conforms to the needs for cultivating students’ competences; however, definitive methods of analysis that can confirm the correlation between competences and curricula are lacking. The purpose of this research was to introduce a qualityimprovement approach of core competence and curricula for student employment that could be used to continuously review the curricula for information management. The research methods included the establishment of core competences via behavioural event interviews, focus groups and the investigative research method, as well as an exploration of the curriculum for student employment used intechnological universities and the establishment of a quality improvement approach based on Grey Relational Analysis (GRA). The research obtained six items of core competences and six types of curricula for employment, and established a quality improvement approach for core competence and curricula based on the results ofGRA.
Changing the values of parameters on lot size reorder point model
Chang Hung-Chi
Yugoslav Journal of Operations Research , 2003, DOI: 10.2298/yjor0301069c
Abstract: The Just-In-Time (JIT) philosophy has received a great deal of attention. Several actions such as improving quality, reducing setup cost and shortening lead time have been recognized as effective ways to achieve the underlying goal of JIT. This paper considers the partial backorders, lot size reorder point inventory system with an imperfect production process. The objective is to simultaneously optimize the lot size, reorder point, process quality, setup cost and lead time, constrained on a service level. We assume the explicit distributional form of lead time demand is unknown but the mean and standard deviation are given. The minimax distribution free approach is utilized to solve the problem and a numerical example is provided to illustrate the results. .
Interactive E-diagnosis: An Efficient Scheme for Medical Diagnosis Support System
Rong-Chi Chang
Journal of Networks , 2011, DOI: 10.4304/jnw.6.5.713-720
Abstract: With advancements in information technology, computer applications have now been largely implemented in health care, where medical support system or expert system is serving as a second opinion for medical personnel, underscoring its importance. The present study aims to design a prototype medical diagnostic support system, in which DICOM-based image analysis algorithms are utilized to develop an image browser and graphical user interface (GUI), allowing medical personnel to read X-ray, CT scan, MRI or other medical imaging files via a simple browser interface. Practitioners may also configure parameters for the intensity slicing and intensity analysis based on their medical expertise and experience to automatically generate converted imaging results for use as diagnostic references. Furthermore, the integration of GUI and network programming technology facilitates patient consultation and discussions among doctors in different areas, making this medical diagnosis system a telemedicine application, as well as an e-learning tool.
有机化学 , 1993,
Abstract: 发现了一些细菌血红蛋白卟啉辅基,它们的结构与无处不在的原血红蛋白素。这些新色素在芳核和侧链包含有引人注目的改性,样品有血红蛋白素α,ο和α1,讨论了这些大环的合成途径和它们可能的生物合成途径。
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