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Search Results: 1 - 10 of 325587 matches for " IDALIA; SáNCHEZ-PORRAS "
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Evaluación in vivo de la actividad antimalárica de 25 plantas provenientes de una Reserva de Conservación Biológica de Costa Rica In vivo evaluation of the antimalarial activity of 25 plants from a Biological Conservation Reserve of Costa Rica
MISAEL CHINCHILLA-CARMONA,IDALIA VALERIO-CAMPOS,RONALD SáNCHEZ-PORRAS,VíCTOR MORA-CHAVES
Revista chilena de historia natural , 2011,
Abstract: Se realizó una evaluación in vivo de la actividad antimalárica de las hojas, flores, frutos, corteza y raíz de 25 plantas de la Reserva Biológica Alberto Manuel Brenes (REBAMB), situada en San Ramón, Alajuela, Costa Rica. Las plantas estudiadas fueron Aphelandra aurantiaca (Scheidw.) Lindl., Aphelandra tridentata Hemsl. (Acanthaceae), Xanthosoma undipes (K. Koch & C.D. Bouché) K. Koch. (Araceae), Iriartea deltoidea Ruiz & Pav. (Arecaceae), Neurolaena lobata (L.) Cass. (Asteraceae), Lonchocarpus pentaphyllus (Poir.) Kunth ex DC., Pterocarpus hayesii Hemsl., Senna papillosa (Britton & Rose) H.S. Irwin & Barneby., Cinnamomum chavarrianum (Hammel) Kosterm. (Fabaceae), Nectandra membranacea (Sw.) Griseb., Persea povedae W.C. Burger. (Lauraceae), Hampea appendiculata (Donn. Sm.) Standl. (Malvaceae), Guarea glabra Vahl., Ruagea glabra Triana & Planch. (Meliaceae), Psidium guajava L. (Myrtaceae), Bocconia frutescens L. (Papaveraceae), Piper friedrichsthalii C. DC. (Piperaceae), Clematis dioica L. (Ranunculaceae), Prunus annularis Koehne. (Rosaceae), Siparuna thecaphora (Poepp. & Endl.) A. DC. (Siparunaceae), Solanum arboreum Dunal., Witheringia solanacea L'Hér. (Solanaceae), Ticodendron incognitum Gómez-Laur. & L.D. Gómez. (Ticodendraceae), Heliocarpus appendiculatus Turcz. (Tiliaceae) y Myriocarpa longipes Liebm. (Urticaceae). Los extractos alcohólicos frescos y secos, fueron evaluados por su actividad inhibitoria de la parasitemia causada por Plasmodium berghei en ratones Swiss. Al realizar las prueba de CI50 las plantas en que esa actividad fue muy relevante fueron (en mg kg-1 de peso): 12 para la corteza de B. frutescens, 18 para la raíz de H. appendiculata, 14 para la raíz de I. deltoidea, 4 para el fruto inmaduro de M. longipes, 21 para la raíz de N. membranacea, 19 para las hojas tiernas de P. povedae y 16 para el fruto inmaduro de S. tecaphora. Los extractos frescos presentaron una mayor actividad antimalárica que los sometidos a desecación. Este estudio es una contribución más al conocimiento del valor potencial farmacológico de la biodiversidad botánica costarricense. An evaluation of the antimalarial activity of the leaves, flowers, fruits, bark and roots of 25 plants from the Reserve Alberto Manuel Brenes Biological (REBAMB) was performed. The reserve is located in San Ramon, Alajuela, Costa Rica and the plants studied were Aphelandra aurantiaca (Scheidw.) Lindl., Aphelandra tridentata Hemsl. (Acanthaceae), Xanthosoma undipes (K. Koch & C.D. Bouché) K. Koch. (Araceae), Iriartea deltoidea Ruiz & Pav. (Arecaceae), Neurolaena lobata (L.) Cass. (Asterace
Evaluación in vivo de la actividad antimalárica de 25 plantas provenientes de una Reserva de Conservación Biológica de Costa Rica
CHINCHILLA-CARMONA,MISAEL; VALERIO-CAMPOS,IDALIA; SáNCHEZ-PORRAS,RONALD; MORA-CHAVES,VíCTOR; BAGNARELLO-MADRIGAL,VANESSA; MARTíNEZ-ESQUIVEL,LAURA; GONZáLEZ-PANIAGUA,ANTONIETA; VANEGAS,JUAN CARLOS;
Revista chilena de historia natural , 2011, DOI: 10.4067/S0716-078X2011000100009
Abstract: an evaluation of the antimalarial activity of the leaves, flowers, fruits, bark and roots of 25 plants from the reserve alberto manuel brenes biological (rebamb) was performed. the reserve is located in san ramon, alajuela, costa rica and the plants studied were aphelandra aurantiaca (scheidw.) lindl., aphelandra tridentata hemsl. (acanthaceae), xanthosoma undipes (k. koch & c.d. bouché) k. koch. (araceae), iriartea deltoidea ruiz & pav. (arecaceae), neurolaena lobata (l.) cass. (asteraceae), lonchocarpus pentaphyllus (poir.) kunth ex dc., pterocarpus hayesii hemsl., senna papillosa (britton & rose) h.s. irwin & barneby., cinnamomum chavarrianum (hammel) kosterm. (fabaceae), nectandra membranacea (sw.) griseb., persea povedae w.c. burger. (lauraceae), hampea appendiculata (donn. sm.) standl. (malvaceae), guarea glabra vahl., ruagea glabra triana & planch. (meliaceae), psidium guajava l. (myrtaceae), bocconia frutescens l. (papaveraceae), piper friedrichsthalii c. dc. (piperaceae), clematis dioica l. (ranunculaceae), prunus annularis koehne. (rosaceae), siparuna thecaphora (poepp. & endl.) a. dc. (siparunaceae), solanum arboreum dunal., witheringia solanacea l'hér. (solanaceae), ticodendron incognitum gómez-laur. & l.d. gómez. (ticodendraceae), heliocarpus appendiculatus turcz. (tiliaceae) y myriocarpa longipes liebm. (urticaceae). the fresh and dry alcoholic extracts were evaluated in swiss mice for their inhibitory activity on multiplication of plasmodium berghei. when making the test ic50, the only plants whose activity (mg kg-1 of body weight) was relevant were: 12 for bark in b. frutescens, 18 for root in h. appendiculata, 14 for root in i. deltoidea, 4 for unripe fruits in m. longipes, 21 for root in n. membranacea, 19 for young leaves in p. povedae and 16 for unripe fruits in s. tecaphora. the fresh extracts showed greater antimalarial activity than those previously dried. this study is a contribution to the knowledge of potential medicinal value of botanical
In vitro antimalarial activity of extracts of some plants from a biological reserve in Costa Rica
Misael Chinchilla,Idalia Valerio,Ronald Sánchez,Víctor Mora
Revista de Biología Tropical , 2012,
Abstract: Treatment with the usual antimalarial drugs, have induced parasite resistance, reinforcing the need to finding natural antimalarial components that would be found on plants from the forest. Therefore, we decided to look for these components in Costa Rican plants from a protected forest area. Fresh and dry extracts of roots, bark, leaves, flowers and fruits of 25 plants from a biological reserve in Costa Rica, Reserva Biológica Alberto Manuel Brenes (REBAMB), were studied in vitro for the presence of substances with antimalarial activity. By studying the inhibition of P. berghei schizogony, we assessed the antimalarial activity of several plant extracts: Aphelandra aurantiaca, A. tridentata (Acanthaceae); Xanthosoma undipes (Araceae); Iriartea deltoidea (Arecaceae); Neurolaena lobata (Asteraceae); Senna papillosa, Pterocarpus hayessi, Lonchocarpus pentaphyllus (Fabaceae); Nectandra membranacea, Persea povedae, Cinamomum chavarrianum (Lauraceae); Hampea appendiculata (Malvaceae); Ruagea glabra, Guarea glabra (Meliaceae); Psidium guajava (Myrtaceae); Bocconia frutescens (Papaveraceae); Piper friedrichsthalii (Piperaceae); Clematis dioica (Ranunculaceae); Prunus annularis (Rosaceae); Siparuna thecaphora (Siparunaceae); Solanum arboreum, Witheringia solanácea (Solanaceae); Ticodendrum incognitum (Ticodendraceae); Heliocarpus appendiculatus (Tiliaceae) and Myriocarpa longipes (Urticaceae). We used different parts of the plants as well as fresh and dried extracts for testing IC50. The solid content of the extracts ranged from 1-71.9μg/mL. The fresh extracts showed stronger activity than the dry ones. Since the plants showing the strongest antimalarial activity are very common in Central America, and some similar genera of these plants have shown positives results in South America, we considered important to present these findings for discussion. On the other hand, this is the first systematic study of this kind ever realized in a circumscribed and protected area of Costa Rica. Rev. Biol. Trop. 60 (2): 881-891. Epub 2012 June 01. El tratamiento con las drogas antimaláricas de uso común han inducido resistencia por parte del parásito, lo que obliga a buscar en las plantas de los bosques, componentes naturales con actividad en contra de esta enfermedad. Por lo tanto, decidimos buscar dichos componentes en plantas de una Reserva Forestal de Costa Rica. Extractos tanto frescos como secos de raíz, corteza, hojas, flores y frutos, de 25 plantas de la Reserva Biológica Alberto Manuel Brenes (REBAMB), fueron estudiados in vitro en busca de sustancias con actividad antim
Parásitos en monos carablanca Cebus capucinus (Primates: Cebidae) de Costa Rica
CHINCHILLA,MISAEL; GUERRERO,OLGA M; GUTIERREZ-ESPELETA,GUSTAVO A; SáNCHEZ,RONALD; VALERIO CAMPOS,IDALIA;
Parasitología latinoamericana , 2007, DOI: 10.4067/S0717-77122007000200011
Abstract: fecal samples of 53 white face monkeys (cebus capucinus) from some sites of costa rica were studied for blood and intestinal parasites. animals were anesthetized with darts containing telazol, blood and fecal samples were collected and all the material was studied in the laboratory. for blood parasites, giemsa stain andknott concentration was performed. intestinal parasites were studied by direct examination in 0,85% saline solution and a iodine solution. haematoxylin stain was used for better protozoa identification. strongyloides sp, hookworms, acanthocephalid eggs and other nematodes, as well as tritrichomonas sp (more frequent) and other protozoa were found. the presence of at least one parasites was observed in 33.3% to 100% of the fecal samples with an average of 73.6%. there was not any correlation between sex and infection rate, but the presence of parasite was higher in heavier (older) animals. microfllarias were the only blood organism detected.the reasons for these high infection rates could be explained for feed diversity, contaminated soil and water contact and sociability of these animals, among other factors.
Seguimiento a patógenos presentes en biosólido empleado como enmienda para revegetalizar un talud
López Sánchez,Idalia Jacqueline; Acevedo Cifuentes,Diana Rocío; Ordó?ez Ante,Carlos Andrés;
Revista Ingenierías Universidad de Medellín , 2010,
Abstract: in order to evaluate the feasibility of biosolids use as an organic emendation for reforestation and controlling active superficial erosive processes, a part of the highway -located near to the alternative route to caldas (antioquia). with the purpose of preventing erosion, two types of plants brachiaria decumbens and kikuyu grass (pennisetum clandestinum) were planted, using biosolids from san fernando water treatment plant, mixed with soil from the site. the behavior of some environmental risk bioindicators: total coliforms, fecal coliforms, salmonella-shiguella, were evaluated, in runoff waters and in the slope soil across the time. both plant species adapted well to soil; the coverage was complete and permanent. results show the feasibility of bio-solid as emendation. however, microbiological specifications measured during the evaluation time, showed the need for sanitizing the bio-solid before mixing it with soil, since the presence of these pathogens represents an environmental risk.
Seguimiento a patógenos presentes en biosólido empleado como enmienda para revegetalizar un talud Follow-up to pathogens present in biosolids used as emendation to reforest a slope
Idalia Jacqueline López Sánchez,Diana Rocío Acevedo Cifuentes,Carlos Andrés Ordó?ez Ante
Revista Ingenierías Universidad de Medellín , 2010,
Abstract: Con el fin de evaluar la factibilidad del uso del biosólido como enmienda orgánica para el establecimiento de vegetación y el control de procesos erosivos superficiales activos, se seleccionó un corte de carretera ubicado sobrela Variante a Caldas (Antioquia). Para darle amarre y cobertura al suelo, se sembraron dos especies vegetales tipo pasto Brachiaria Decumbens y Kikuyo (Pennisetum clandestinum), utilizando biosólido proveniente de la PTAR San Fernando, mezclado con suelo de la zona. Se evaluó el comportamiento de bioindicadores de riesgo ambiental: coliformes totales, coliformes fecales, Salmonella-Shiguella, en las aguas de escorrentía y en el suelo del talud en el tiempo. Ambas especies se adaptaron bien amarrando el suelo; la cobertura fue total y permanente. Los resultados muestran la factibilidad del uso del biosólido como enmienda. Sin embargo, la permanencia de los parámetros microbiológicos medidos durante el tiempo de evaluación evidencia la necesidad de sanitizar el biosólido antes de usarlo, ya que estos patógenos constituyen un riesgo ambiental. In order to evaluate the feasibility of biosolids use as an organic emendation for reforestation and controlling active superficial erosive processes, a part of the highway -located near to the alternative route to Caldas (Antioquia). With the purpose of preventing erosion, two types of plants Brachiaria Decumbens and kikuyu grass (Pennisetum clandestinum) were planted, using biosolids from San Fernando Water Treatment Plant, mixed with soil from the site. The behavior of some environmental risk bioindicators: total coliforms, fecal coliforms, Salmonella-Shiguella, were evaluated, in runoff waters and in the slope soil across the time. Both plant species adapted well to soil; the coverage was complete and permanent. Results show the feasibility of bio-solid as emendation. However, microbiological specifications measured during the evaluation time, showed the need for sanitizing the bio-solid before mixing it with soil, since the presence of these pathogens represents an environmental risk.
Dinamización Turística de la Cuenca Minera de Riotinto (Huelva, Espa?a)
Vargas Sánchez,Alfonso; Romero Macías,Emilio; Porras Bueno,Nuria; Plaza Mejía,Ma ángeles;
Visi?3n de futuro , 2009,
Abstract: in this paper the case of the mining river basin of the province of huelva is treated (spain), centered in the potentialities of its pyrites strip, mainly as from, the resources of the locality of minas riotinto and of the initiatives carried out there. the giving of value of that mining, tangible and intangible patrimony, with a tourist purpose has to be done as from the knowledge of the behavior of the tourist and the key matters that determine their satisfaction. to this end, the results of the field work carried out among the visitors to the riotinto parque minero are exposed.
Prevalencia de Infección por VIH en Pacientes con Patología Dermatológica en un Centro de Referencia Nacional 2005-2006
Porras de Quintana,Luisa; Tamayo-Buendía,Margarita; Sánchez-Vanegas,Guillermo;
Revista de Salud Pública , 2009, DOI: 10.1590/S0124-00642009000400012
Abstract: objective determining the prevalence of hiv infection in patients suffering from cutaneous disease in a national reference center in colombia. methods this was a cross-sectional study of patients aged 18 or older, having cutaneous pathology and no previous hiv diagnosis. the calculated sample size was 1,537 patients. a confirmed case was defined as being that of a patient having positive results in the elisa and western blot tests. prevalence was the number of confirmed cases divided by the total population. the associations were measured by using prevalence ratio. results 1,649 patients were enrolled in the study. our center’s prevalence was 1.21 % (20 cases). molluscum contagiosum and seborrheic dermatitis were the diseases having the highest prevalence of hiv infection which had not been previously diagnosed (3.91 % and 2.05 %, respectively). molluscum contagiosum and hiv infection prevalence ratio was 3.96 (p: 0.01562). conclusions the risk of hiv infection in patients aged 18 or older suffering from molluscum contagiosum was nearly 4 times higher than the risk in patients having other cutaneous pathologies.
Cáncer de Piel: Perfil Epidemiológico de un Centro de Referencia en Colombia 2003-2005
Nova-Villanueva,John; Sánchez-Vanegas,Guillermo; Porras de Quintana,Luisa;
Revista de Salud Pública , 2007, DOI: 10.1590/S0124-00642007000400012
Abstract: skin cancer cases were identified from 2003-2005 to ascertain the epidemiological profile and incidence of skin cancer at the centro dermatológico federico lleras acosta-colombia. 168 659 confirmed diagnoses were registered between 2003 and 2005, 2 184 corresponding to skin cancer. basal cell carcinoma increased from 4 per 1 000 in 2003 to 11 per 1 000 in 2005. patients suffering from squamous cell carcinoma increased from 1 per 1 000 in 2003 to 3 per 1 000 in 2005. cases of melanoma increased from 2,7 per 10 000 in 2003 to 13 per 10 000 in 2005.
Parásitos intestinales en monos congo Alouatta palliata (Primates: Cebidae) de Costa Rica
Misael Chinchilla Carmona,Olga Guerrero Bermúdez,Gustavo A Gutiérrez- Espeleta,Ronald Sánchez Porras
Revista de Biología Tropical , 2005,
Abstract: Se estudiaron las heces de 102 monos de la especie Alouatta palliata (monos aulladores o congos) de las siguientes regiones de Costa Rica. Valle Central: San Ramón (Alajuela); Pacífico Central: Isla Chira y Parque Manuel Antonio (Puntarenas); Pacífico Norte: Palo Verde, Playa Potrero, Nicoya (Guanacaste) y Caribe: Cahuita (Limón). Los animales capturados fueron anestesiados con dardos conteniendo Telazol . La mayoría defecaron espontáneamente o se les estimulaba a defecar. Las muestras se estudiaron en el laboratorio en forma directa en solución salina (0.85%) y una solución de yodo o te idas con hematoxilina para identificar los parásitos correspondientes. El material fue cultivado en el medio de Dobell para determinar la presencia de amebas y flagelados. Se encontraron helmintos de los géneros Trypanoxyuris, Strongyloides y Controrchis y protozoarios de los géneros Endolimax, Entamoeba, Trichomonas y Giardia. Los porcentajes de infección por protozoarios oscilaron entre 16.7% y 80% y se encontraron helmintos en un 3.4% a un 80% de los animales de acuerdo con la zona estudiada. No se encontraron diferencias importantes de parasitosis relacionadas con el sexo pero si existió mayor infección en los animales más jóvenes. Se establece la relación entre los índices de infección y algunos parámetros tales como las áreas estudiadas, el peso de los animales y el contacto con el ser humano. Intestinal parasites in howler monkeys Alouatta palliata (Primates: Cebidae) of Costa Rica. Fecal samples of 102 howler monkeys (Alouatta palliata) from several sites of Costa Rica were studied for intestinal parasites. The zones studied were: Central Valley (San Ramón, Alajuela), Central Pacific (Chomes and Manuel Antonio National Park, Puntarenas), North Pacific (Palo Verde Park and Playa Potrero, Guanacaste), Chira Island in the Nicoya Gulf and Caribean area (Cahuita, Limón). Animals were anesthetized with dards containing Telazol in order to collect the fecal material; some monkeys defecated spontaneously and others by direct stimulation. Samples were studied in saline solution (0.85%) and a Iodine solution, or stained with Haematoxylin. The material was also cultured in Dobell culture medium to determine the presence of amoeba and flagellates. Strongyloides, Controrchis, Trypanoxyuris genera were found in 3.4% of the samples. In addition 16.7% to 80% of the animals showed protozoa infection with Endolimax, Entamoeba, Trichomonas and Giardia. It is discussed the relationships of parasite infection with environmental conditions, animal population and human presence, speci
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