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Search Results: 1 - 10 of 127076 matches for " Huixian Li "
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Virus-induced gene-silencing in wheat spikes and grains and its application in functional analysis of HMW-GS-encoding genes
Meng Ma, Yan Yan, Li Huang, Mingshun Chen, Huixian Zhao
BMC Plant Biology , 2012, DOI: 10.1186/1471-2229-12-141
Abstract: Apparent photobleaching on the spikes infected with BSMV:PDS at heading stage was observed after13 days post inoculation (dpi), and persisted until 30dpi, while the spikes inoculated with BSMV:00 remained green during the same period. Grains of BSMV:PDS infected spikes also exhibited photobleaching. Molecular analysis indicated that photobleached spikes or grains resulted from the reduction of endogenous PDS transcript abundances, suggesting that BSMV:PDS was able to induce PDS silencing in wheat spikes and grains. Inoculation onto wheat spikes from heading to flowering stage was optimal for efficient silencing of PDS in wheat spikes. Furthermore, we used the BSMV-based system to reduce the transcript level of 1Bx14, a gene encoding for High-molecular-weight glutenin subunit 1Bx14 (HMW-GS 1Bx14), by 97?% in the grains of the BSMV:1Bx14 infected spikes at 15dpi, compared with that in BSMV:00 infected spikes, and the reduction persisted until at least 25 dpi. The amount of the HMW-GS 1Bx14 was also detectably decreased. The percentage of glutenin macropolymeric proteins in total proteins was significantly reduced in the grains of 1Bx14-silenced plants as compared with that in the grains of BSMV:00 infected control plants, indicating that HMW-GS 1Bx14 is one of major components participating in the formation of glutenin macropolymers in wheat grains.This is one of the first reports of successful application of BSMV-based virus-induced-gene-silencing (VIGS) for gene knockdown in wheat spikes and grains and its application in functional analysis of the 1Bx14 gene. The established BSMV-VIGS system will be very useful in future research on functional analysis of genes contributing to grain quality and the metabolic networks in developing seeds of wheat.Wheat (Triticum aestivum L.) is one of the major staple crops for the human diet. With the increase in the global population, the shortage of foods has become more and more serious. Therefore, wheat yield and quality improve
Quantum Attack-Resistent Certificateless Multi-Receiver Signcryption Scheme
Huixian Li, Xubao Chen, Liaojun Pang, Weisong Shi
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0049141
Abstract: The existing certificateless signcryption schemes were designed mainly based on the traditional public key cryptography, in which the security relies on the hard problems, such as factor decomposition and discrete logarithm. However, these problems will be easily solved by the quantum computing. So the existing certificateless signcryption schemes are vulnerable to the quantum attack. Multivariate public key cryptography (MPKC), which can resist the quantum attack, is one of the alternative solutions to guarantee the security of communications in the post-quantum age. Motivated by these concerns, we proposed a new construction of the certificateless multi-receiver signcryption scheme (CLMSC) based on MPKC. The new scheme inherits the security of MPKC, which can withstand the quantum attack. Multivariate quadratic polynomial operations, which have lower computation complexity than bilinear pairing operations, are employed in signcrypting a message for a certain number of receivers in our scheme. Security analysis shows that our scheme is a secure MPKC-based scheme. We proved its security under the hardness of the Multivariate Quadratic (MQ) problem and its unforgeability under the Isomorphism of Polynomials (IP) assumption in the random oracle model. The analysis results show that our scheme also has the security properties of non-repudiation, perfect forward secrecy, perfect backward secrecy and public verifiability. Compared with the existing schemes in terms of computation complexity and ciphertext length, our scheme is more efficient, which makes it suitable for terminals with low computation capacity like smart cards.
Finite-Time Control for Permanent Magnet Synchronous Motor Position Tracking System
永磁同步电机伺服系统的有限时间位置控制

LIU Huixian,WANG Zhao,LI Shihua,
刘慧贤
,王钊,李世华

系统科学与数学 , 2010,
Abstract: This paper investigates the problem of position tracking of Permanent Magnet Synchronous Motor (PMSM) servo system. On the basis of analyzing the mathematical model of the PMSM, the exact linearization and decoupling of the motor model can be achieved by using the feedback-linearization technique. First, the PMSM position tracking system is transformed to two linear control subsystems by using the feedback linearization. The corresponding finite-timecontrollers are designed respectively for the two subsystems, and a load estimator based on finite time control is developed to observe and estimate the external load disturbance. The analysis of stability is given for the PMSM closed-loop system. Compared with the corresponding asymptotically stable control method, the scheme based on the finite-time control can track the desired positionsignal in finite-time and obtain a better dynamic response and anti-disturbance performance. The simulation results illustrate the effectiveness of the control scheme.
Cytological and Comparative Proteomic Analyses on Male Sterility in Brassica napus L. Induced by the Chemical Hybridization Agent Monosulphuron Ester Sodium
Yufeng Cheng, Qian Wang, Zhanjie Li, Jianmin Cui, Shengwu Hu, Huixian Zhao, Mingshun Chen
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0080191
Abstract: Male sterility induced by a chemical hybridization agent (CHA) is an important tool for utilizing crop heterosis. Monosulphuron ester sodium (MES), a new acetolactate synthase-inhibitor herbicide belonging to the sulphonylurea family, has been developed as an effective CHA to induce male sterility in rapeseed (Brassica napus L.). To understand MES-induced male sterility in rapeseed better, comparative cytological and proteomic analyses were conducted in this study. Cytological analysis indicated that defective tapetal cells and abnormal microspores were gradually generated in the developing anthers of MES-treated plants at various development stages, resulting in unviable microspores and male sterility. A total of 141 differentially expressed proteins between the MES-treated and control plants were revealed, and 131 of them were further identified by MALDI-TOF/TOF MS. Most of these proteins decreased in abundance in tissues of MES-treated rapeseed plants, and only a few increased. Notably, some proteins were absent or induced in developing anthers after MES treatment. These proteins were involved in several processes that may be crucial for tapetum and microspore development. Down-regulation of these proteins may disrupt the coordination of developmental and metabolic processes, resulting in defective tapetum and abnormal microspores that lead to male sterility in MES-treated plants. Accordingly, a simple model of CHA-MES-induced male sterility in rapeseed was established. This study is the first cytological and dynamic proteomic investigation on CHA-MES-induced male sterility in rapeseed, and the results provide new insights into the molecular events of male sterility.
The ATP-Binding Cassette Transporter ABCB19 Regulates Postembryonic Organ Separation in Arabidopsis
Hongtao Zhao, Lei Liu, Huixian Mo, Litao Qian, Ying Cao, Sujuan Cui, Xia Li, Ligeng Ma
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0060809
Abstract: The phytohormone auxin plays a critical role in plant development, including embryogenesis, organogenesis, tropism, apical dominance and in cell growth, division, and expansion. In these processes, the concentration gradient of auxin, which is established by polar auxin transport mediated by PIN-FORMED (PIN) proteins and several ATP-binding cassette/multi-drug resistance/P-glycoprotein (ABCB/MDR/PGP) transporters, is a crucial signal. Here, we characterized the function of ABCB19 in the control of Arabidopsis organ boundary development. We identified a new abcb19 allele, abcb19-5, which showed stem-cauline leaf and stem-pedicel fusion defects. By virtue of the DII-VENUS marker, the auxin level was found to be increased at the organ boundary region in the inflorescence apex. The expression of CUP-SHAPED COTYLEDON2 (CUC2) was decreased, while no obvious change in the expression of CUC3 was observed, in abcb19. In addition, the fusion defects were greatly enhanced in cuc3 abcb19-5, which was reminiscent of cuc2 cuc3. We also found that some other organ boundary genes, such as LOF1/2 were down-regulated in abcb19. Together, these results reveal a new aspect of auxin transporter ABCB19 function, which is largely dependent on the positive regulation of organ boundary genes CUC2 and LOFs at the postembryonic organ boundary.
Approximate Inertial Manifold for a Class of the Kirchhoff Wave Equations with Nonlinear Strongly Damped Terms  [PDF]
Chengfei Ai, Huixian Zhu, Guoguang Lin
International Journal of Modern Nonlinear Theory and Application (IJMNTA) , 2016, DOI: 10.4236/ijmnta.2016.54020
Abstract: This paper is devoted to the long time behavior of the solution to the initial boundary value problems for a class of the Kirchhoff wave equations with nonlinear strongly damped terms: \"\". Firstly, in order to prove the smoothing effect of the solution, we make efficient use of the analytic property of the semigroup generated by the principal operator of the equation in the phase space. Then we obtain the regularity of the global attractor and construct the approximate inertial manifold of the equation. Finally, we prove that arbitrary trajectory of the Kirchhoff wave equations goes into a small neighbourhood of the approximate inertial manifold after large time.
Individual dispersion of synthetic imogolite nanotubes via droplet evaporation
HuiXian Yang,ZhaoHui Su
Chinese Science Bulletin , 2007, DOI: 10.1007/s11434-007-0305-y
Abstract: Morphology of synthetic imogolite nanotubes formed in droplet evaporation was investigated by transmission electron microscopy and electron diffraction. The nanotubes form a dense entangled network at higher concentrations, while at lower concentrations the nanotubes are liable to form oriented bundles. Under enthanol atmosphere, individual dispersion of nanotubes was observed for the first time, which reveals the length polydispersity of synthetic imogolite nanotubes.
Transcriptional and Post-Transcriptional Mechanisms for Oncogenic Overexpression of Ether à Go-Go K+ Channel
Huixian Lin, Zhe Li, Chang Chen, Xiaobin Luo, Jiening Xiao, Deli Dong, Yanjie Lu, Baofeng Yang, Zhiguo Wang
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0020362
Abstract: The human ether-à-go-go-1 (h-eag1) K+ channel is expressed in a variety of cell lines derived from human malignant tumors and in clinical samples of several different cancers, but is otherwise absent in normal tissues. It was found to be necessary for cell cycle progression and tumorigenesis. Specific inhibition of h-eag1 expression leads to inhibition of tumor cell proliferation. We report here that h-eag1 expression is controlled by the p53?miR-34?E2F1 pathway through a negative feed-forward mechanism. We first established E2F1 as a transactivator of h-eag1 gene through characterizing its promoter region. We then revealed that miR-34, a known transcriptional target of p53, is an important negative regulator of h-eag1 through dual mechanisms by directly repressing h-eag1 at the post-transcriptional level and indirectly silencing h-eag1 at the transcriptional level via repressing E2F1. There is a strong inverse relationship between the expression levels of miR-34 and h-eag1 protein. H-eag1antisense antagonized the growth-stimulating effects and the upregulation of h-eag1 expression in SHSY5Y cells, induced by knockdown of miR-34, E2F1 overexpression, or inhibition of p53 activity. Therefore, p53 negatively regulates h-eag1 expression by a negative feed-forward mechanism through the p53?miR-34?E2F1 pathway. Inactivation of p53 activity, as is the case in many cancers, can thus cause oncogenic overexpression of h-eag1 by relieving the negative feed-forward regulation. These findings not only help us understand the molecular mechanisms for oncogenic overexpression of h-eag1 in tumorigenesis but also uncover the cell-cycle regulation through the p53?miR-34?E2F1?h-eag1 pathway. Moreover, these findings place h-eag1 in the p53?miR-34?E2F1?h-eag1 pathway with h-eag as a terminal effecter component and with miR-34 (and E2F1) as a linker between p53 and h-eag1. Our study therefore fills the gap between p53 pathway and its cellular function mediated by h-eag1.
Cd uptake in rice cultivars treated with organic acids and EDTA

Weihong Xu Yangrui Li Jianping He Qifu Ma Xiaojing Zhang Guiqing Chen Huixian Wang Haibo Zhang,

环境科学学报(英文版) , 2010,
Abstract: A pot experiment was conducted to examine the activity of antioxidant enzymes, the content of malondialdehyde (MDA), proline and protein, and Cd uptake in different rice cultivars exposed to Cd (0, 1 and 5 mg/kg) in the presence of organic acids and ethylenediamine tetraacetic acid (EDTA). The results showed the increase in activity of dismutase (SOD), contents of proline and protein but a decline in activities of peroxidase (POD) and catalase (CAT), and MDA content for cultivars Xiushui63 and IIyou527. The...
Outflows and Bubbles in Taurus: Star-formation Feedback Sufficient to Maintain Turbulence
Huixian Li,Di Li,Lei Qian,Duo Xu,Paul F. Goldsmith,Alberto Noriega-Crespo,Yuefang Wu,Yuzhe Song,Rendong Nan
Physics , 2015, DOI: 10.1088/0067-0049/219/2/20
Abstract: We have identified outflows and bubbles in the Taurus molecular cloud based on the $\sim 100$ deg$^2$ Five College Radio Astronomy Observatory $^{12}$CO(1-0) and $^{13}$CO(1-0) maps and the Spitzer young stellar object catalogs. In the main 44 deg$^2$ area of Taurus we found 55 outflows, of which 31 were previously unknown. We also found 37 bubbles in the entire 100 deg$^2$ area of Taurus, all of which had not been found before. The total kinetic energy of the identified outflows is estimated to be $\bf \sim 3.9 \times 10^{45}$ erg, which is \textbf{1\%} of the cloud turbulent energy. The total kinetic energy of the detected bubbles is estimated to be $\sim 9.2 \times 10^{46}$ erg, which is 29\% of the turbulent energy of Taurus. The energy injection rate from outflows is $\bf \sim 1.3 \times 10^{33}~\rm erg\ s^{-1}$, \textbf{0.4 - 2 times} the dissipation rate of the cloud turbulence. The energy injection rate from bubbles is $\sim 6.4 \times 10^{33}$ erg s$^{-1}$, \textbf{2 - 10 times} the turbulent dissipation rate of the cloud. The gravitational binding energy of the cloud is $\bf \sim 1.5 \times 10^{48}$ {\bf erg}, \textbf{385} and 16 times the energy of outflows and bubbles, respectively. We conclude that neither outflows nor bubbles can \textbf{provide enough energy to balance the overall gravitational binding energy and the turbulent energy of Taurus. However,} in the current epoch, stellar feedback is sufficient to maintain the observed turbulence in Taurus.
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