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Search Results: 1 - 10 of 36868 matches for " Huihuang Yan "
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Analysis and location of a rice BAC clone containing telomeric DNA sequences
Wenxue Zhai,Hao Chen,Huihuang Yan,Changjie Yan,Guoliang Wang,Lihuang Zhu
Science China Life Sciences , 1999, DOI: 10.1007/BF02881750
Abstract: BAC2, a rice BAC clone containing (TTTAGGG)n homologous sequences, was analyzed by Southern hybridization and DNA sequencing of its subclones. It was disclosed that there were many tandem repeated satellite DNA sequences, called TA352, as well as simple tandem repeats consisting of TTTAGGG or its variant within the BAC2 insert. A 0. 8 kb (TTTAGGG)n-containing fragment in BAC2 was mapped in the telomere regions of at least 5 pairs of rice chromosomes by using fluorescencein situ hybridization (FISH). By RFLP analysis of low copy sequences the BAC2 clone was localized in one terminal region of chromosome 6. All the results strongly suggest that the telomeric DNA sequences of rice are TTTAGGG or its variant, and the linked satellite DNA TA352 sequences belong to telomere-associated sequences.
Analysis and location of a rice BAG clone containing telomeric DNA sequences

ZHAI Wenxue CHEN Hao YAN Huihuang YAN Changjie WANG GuoliangZHU Lihuang,

中国科学C辑(英文版) , 1999,
Abstract: BAC2, a rice BAC clone containing (TTTAGGG)n homologous sequences, was analyzed by Southern hybridization and DNA sequencing of its subclones. It was disclosed that there were many tandem repeated satellite DNA sequences, called TA352, as well as simple tandem repeats consisting of TTTAGGG or its variant within the BAC2 insert. A 0. 8 kb (TTTAGGG)n-containing fragment in BAC2 was mapped in the telomere regions of at least 5 pairs of rice chromosomes by using fluorescencein situ hybridization (FISH). By RFLP analysis of low copy sequences the BAC2 clone was localized in one terminal region of chromosome 6. All the results strongly suggest that the telomeric DNA sequences of rice are TTTAGGG or its variant, and the linked satellite DNA TA352 sequences belong to telomere-associated sequences.
Mapping of a new gene for brown planthopper resistance in cultivated rice introgressed fromOryza eichingeri
Guoqing Liu,Huihuang Yan,Qiang Fu,Qian Qian,Zhitao Zhang,Wenxue Zhai,Lihuang Zhu
Chinese Science Bulletin , 2001, DOI: 10.1007/BF03187031
Abstract: Wild rice species is an important source of useful genes for cultivated rice improvement. Some accessions ofOryza eichingeri (2n = 24, CC) from Africa confer strong resistance to brown planthopper (BPH), whitebacked planthopper (WBPH) and bacterial blight (BB). In the present study, restriction fragments length polymorphism (RFLP) and simple sequence repeats (SSR) analysis were performed on disomic backcross plants betweenOryza sativa (2n = 24, AA) andO. eichingeri in order to identify the presence ofO. eichingeri segments and further to localize BPH-resistant gene. In the introgression lines, 1–6O. eichingeri segments were detected on rice chromosomes 1, 2, 6, or/and 10. The dominant BPH resistant gene, tentatively named Bph13(t), was mapped to chromosome 2, being 6.1 and 5.5 cM away from two microsatellite markers RM240 and RM250, respectively. The transfer and localization of this gene fromO. eichingeri will contribute to the improvement of BPH resistance in cultivated rice.
Intergenic Locations of Rice Centromeric Chromatin
Huihuang Yan,Paul B. Talbert,Hye-Ran Lee,Jamie Jett,Steven Henikoff,Feng Chen,Jiming Jiang
PLOS Biology , 2012, DOI: 10.1371/journal.pbio.0060286
Abstract: Centromeres are sites for assembly of the chromosomal structures that mediate faithful segregation at mitosis and meiosis. Plant and animal centromeres are typically located in megabase-sized arrays of tandem satellite repeats, making their precise mapping difficult. However, some rice centromeres are largely embedded in nonsatellite DNA, providing an excellent model to study centromere structure and evolution. We used chromatin immunoprecipitation and 454 sequencing to define the boundaries of nine of the 12 centromeres of rice. Centromere regions from chromosomes 8 and 9 were found to share synteny, most likely reflecting an ancient genome duplication. For four centromeres, we mapped discrete subdomains of binding by the centromeric histone variant CENH3. These subdomains were depleted in both intact and nonfunctional genes relative to interspersed subdomains lacking CENH3. The intergenic location of rice centromeric chromatin resembles the situation for human neocentromeres and supports a model of the evolution of centromeres from gene-poor regions.
Intergenic Locations of Rice Centromeric Chromatin
Huihuang Yan,Paul B Talbert,Hye-Ran Lee,Jamie Jett,Steven Henikoff,Feng Chen ,Jiming Jiang
PLOS Biology , 2008, DOI: 10.1371/journal.pbio.0060286
Abstract: Centromeres are sites for assembly of the chromosomal structures that mediate faithful segregation at mitosis and meiosis. Plant and animal centromeres are typically located in megabase-sized arrays of tandem satellite repeats, making their precise mapping difficult. However, some rice centromeres are largely embedded in nonsatellite DNA, providing an excellent model to study centromere structure and evolution. We used chromatin immunoprecipitation and 454 sequencing to define the boundaries of nine of the 12 centromeres of rice. Centromere regions from chromosomes 8 and 9 were found to share synteny, most likely reflecting an ancient genome duplication. For four centromeres, we mapped discrete subdomains of binding by the centromeric histone variant CENH3. These subdomains were depleted in both intact and nonfunctional genes relative to interspersed subdomains lacking CENH3. The intergenic location of rice centromeric chromatin resembles the situation for human neocentromeres and supports a model of the evolution of centromeres from gene-poor regions.
Stereo rectification of calibrated image pairs based on geometric transformation
Huihuang Su,Bingwei He
International Journal of Modern Education and Computer Science , 2011,
Abstract: The objective of stereo rectification is to make the corresponding epipolar lines of image pairs be parallel to the horizontal direction, so that the efficiency of stereo matching is improved as the corresponding points stay in the same horizontal lines of both images. In this paper,a simple and convenient rectification method of calibrated image pairs based on geometric transformation is proposed, which can avoid the complicated calculation of many previous algorithms such as based on epipolar lines, based on fundamental matrix or directly depend on corresponding points. This method is divided into two steps including coordinate system transformation and re-projection of image points. Firstly, we establish two virtual cameras with parallel optical axis by coordinate system transformation based on the pose relationship of the two cameras from calibration result. Secondly, we re-project the points of the original image onto new image planes of the virtual cameras through geometrical method, and then realized the stereo rectification. Experiments of real stereo image pairs show that the proposed method is able to realize the rectification of stereo image pairs accurately and efficiently.
Research on the Customer Value Evaluation System of FX Science and Technology Corporation Ltd
Shujie Zhang,Huihuang Yi
International Journal of Business and Management , 2011, DOI: 10.5539/ijbm.v6n4p214
Abstract: Based on an analysis of the present situation of customer value evaluation of FX Science and Technology Corporation Ltd. in the stream media industry, this paper establishes a customer value evaluation system applicable to this company to evaluate the customer value and subdivide the customers, hence helping the company to integrate the effective resources to the most valuable core customers and the most potential sub-value customers. Through reasonably allocating the resources will a stable long-term customer relationship be established and the customers will participate in the management activities, improving the whole company’s profit-earning ability and competitive ability in an all-round way.
Genome-scale Analysis of Escherichia coli FNR Reveals Complex Features of Transcription Factor Binding
Kevin S. Myers,Huihuang Yan,Irene M. Ong,Dongjun Chung,Kun Liang,Frances Tran,Sündüz Kele?,Robert Landick ,Patricia J. Kiley
PLOS Genetics , 2013, DOI: 10.1371/journal.pgen.1003565
Abstract: FNR is a well-studied global regulator of anaerobiosis, which is widely conserved across bacteria. Despite the importance of FNR and anaerobiosis in microbial lifestyles, the factors that influence its function on a genome-wide scale are poorly understood. Here, we report a functional genomic analysis of FNR action. We find that FNR occupancy at many target sites is strongly influenced by nucleoid-associated proteins (NAPs) that restrict access to many FNR binding sites. At a genome-wide level, only a subset of predicted FNR binding sites were bound under anaerobic fermentative conditions and many appeared to be masked by the NAPs H-NS, IHF and Fis. Similar assays in cells lacking H-NS and its paralog StpA showed increased FNR occupancy at sites bound by H-NS in WT strains, indicating that large regions of the genome are not readily accessible for FNR binding. Genome accessibility may also explain our finding that genome-wide FNR occupancy did not correlate with the match to consensus at binding sites, suggesting that significant variation in ChIP signal was attributable to cross-linking or immunoprecipitation efficiency rather than differences in binding affinities for FNR sites. Correlation of FNR ChIP-seq peaks with transcriptomic data showed that less than half of the FNR-regulated operons could be attributed to direct FNR binding. Conversely, FNR bound some promoters without regulating expression presumably requiring changes in activity of condition-specific transcription factors. Such combinatorial regulation may allow Escherichia coli to respond rapidly to environmental changes and confer an ecological advantage in the anaerobic but nutrient-fluctuating environment of the mammalian gut.
Direct electroless nickel plating on silicon surface
Guanghui Hu,Huihuang Wu,Fangzu Yang
Chinese Science Bulletin , 2004, DOI: 10.1007/BF03183423
Abstract: Direct electroless nickel plating onn-Si(100) wafers in alkaline solutions was demonstrated without any activation procedure in advance, the effect of pH and temperature of the solutions on size of metal particles in deposits was examined, and also the element contents of deposits were analyzed by energy disperse spectroscopy (EDS). The results indicated that the size of metal particles increases with increasing temperature or decreasing pH. The possible mechanism of nickel deposition onn-Si(100) was discussed in terms of semiconductor electrochemistry, and the formation of nickel seed crystal on Si was mainly attributed to the generation of atomic hydrogen by electron capture of water molecule from the semiconductor in alkaline solutions.
ACTIVATED SINTERING OF NATURAL HIGH PURITY MgO-CaO SERIES MATERIALS

ZHANG Zhiping,HUANG Huihuang,LI Guangping,

金属学报 , 1986,
Abstract:
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