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Search Results: 1 - 10 of 58946 matches for " HuiXian Yang "
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Individual dispersion of synthetic imogolite nanotubes via droplet evaporation
HuiXian Yang,ZhaoHui Su
Chinese Science Bulletin , 2007, DOI: 10.1007/s11434-007-0305-y
Abstract: Morphology of synthetic imogolite nanotubes formed in droplet evaporation was investigated by transmission electron microscopy and electron diffraction. The nanotubes form a dense entangled network at higher concentrations, while at lower concentrations the nanotubes are liable to form oriented bundles. Under enthanol atmosphere, individual dispersion of nanotubes was observed for the first time, which reveals the length polydispersity of synthetic imogolite nanotubes.
Approximate Inertial Manifold for a Class of the Kirchhoff Wave Equations with Nonlinear Strongly Damped Terms  [PDF]
Chengfei Ai, Huixian Zhu, Guoguang Lin
International Journal of Modern Nonlinear Theory and Application (IJMNTA) , 2016, DOI: 10.4236/ijmnta.2016.54020
Abstract: This paper is devoted to the long time behavior of the solution to the initial boundary value problems for a class of the Kirchhoff wave equations with nonlinear strongly damped terms: \"\". Firstly, in order to prove the smoothing effect of the solution, we make efficient use of the analytic property of the semigroup generated by the principal operator of the equation in the phase space. Then we obtain the regularity of the global attractor and construct the approximate inertial manifold of the equation. Finally, we prove that arbitrary trajectory of the Kirchhoff wave equations goes into a small neighbourhood of the approximate inertial manifold after large time.
Transcriptional and Post-Transcriptional Mechanisms for Oncogenic Overexpression of Ether à Go-Go K+ Channel
Huixian Lin, Zhe Li, Chang Chen, Xiaobin Luo, Jiening Xiao, Deli Dong, Yanjie Lu, Baofeng Yang, Zhiguo Wang
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0020362
Abstract: The human ether-à-go-go-1 (h-eag1) K+ channel is expressed in a variety of cell lines derived from human malignant tumors and in clinical samples of several different cancers, but is otherwise absent in normal tissues. It was found to be necessary for cell cycle progression and tumorigenesis. Specific inhibition of h-eag1 expression leads to inhibition of tumor cell proliferation. We report here that h-eag1 expression is controlled by the p53?miR-34?E2F1 pathway through a negative feed-forward mechanism. We first established E2F1 as a transactivator of h-eag1 gene through characterizing its promoter region. We then revealed that miR-34, a known transcriptional target of p53, is an important negative regulator of h-eag1 through dual mechanisms by directly repressing h-eag1 at the post-transcriptional level and indirectly silencing h-eag1 at the transcriptional level via repressing E2F1. There is a strong inverse relationship between the expression levels of miR-34 and h-eag1 protein. H-eag1antisense antagonized the growth-stimulating effects and the upregulation of h-eag1 expression in SHSY5Y cells, induced by knockdown of miR-34, E2F1 overexpression, or inhibition of p53 activity. Therefore, p53 negatively regulates h-eag1 expression by a negative feed-forward mechanism through the p53?miR-34?E2F1 pathway. Inactivation of p53 activity, as is the case in many cancers, can thus cause oncogenic overexpression of h-eag1 by relieving the negative feed-forward regulation. These findings not only help us understand the molecular mechanisms for oncogenic overexpression of h-eag1 in tumorigenesis but also uncover the cell-cycle regulation through the p53?miR-34?E2F1?h-eag1 pathway. Moreover, these findings place h-eag1 in the p53?miR-34?E2F1?h-eag1 pathway with h-eag as a terminal effecter component and with miR-34 (and E2F1) as a linker between p53 and h-eag1. Our study therefore fills the gap between p53 pathway and its cellular function mediated by h-eag1.
Unusual conservation among genes encoding small secreted salivary gland proteins from a gall midge
Ming-Shun Chen, Xuming Liu, Ziheng Yang, Huixian Zhao, Richard H Shukle, Jeffrey J Stuart, Scot Hulbert
BMC Evolutionary Biology , 2010, DOI: 10.1186/1471-2148-10-296
Abstract: Seven genes from the SSSGP-1 family are clustered as one inverted and six tandem repeats within a 15 kb region of the genome. Except for SSSGP-1A2, a gene that encodes a protein identical to that encoded by SSSGP-1A1, the other six genes consist of a highly diversified, mature protein-coding region as well as highly conserved regions including the promoter, 5'- and 3'-UTRs, a signal peptide coding region, and an intron. This unusual pattern of highly diversified coding regions coupled with highly conserved regions in the rest of the gene was also observed in several other groups of SSSGP-encoding genes or cDNAs. The unusual conservation pattern was also found in some of the SSSGP cDNAs from the Asian rice gall midge, but not from the orange wheat blossom midge. Strong positive selection was one of the forces driving for diversification whereas concerted homogenization was likely a mechanism for sequence conservation.Rapid diversification in mature SSSGPs suggests that the genes are under selection pressure for functional adaptation. The conservation in the noncoding regions of these genes including introns also suggested potential mechanisms for sequence homogenization that are not yet fully understood. This report should be useful for future studies on genetic mechanisms involved in evolution and functional adaptation of parasite genes.Insect salivary glands are the main organs for producing proteins that are injected into hosts [1]. Plant-feeding insects, especially those with sucking mouthparts, inject proteins and other substances into host plants to facilitate mouthpart penetration, partially digest food before ingestion, and suppress plant defense [2-4]. Substances, including proteins with regulatory roles that can alter host physiology, are referred to as effectors [5]. Pathogens, including bacteria, fungi, oomycetes, and nematodes, deliver various effector proteins into host tissues [5-8]. Substantial evidence suggests that some of the salivary proteins inje
Thermal Transport Properties of Bechgaard Salts (TMTSF)2PF6 and (TMTSF)2ClO4: Implication of Spin-Charge Separation
Yisheng Chai,Hongshun Yang,Jian Liu,Chenghai Sun,Huixian Gao,Xudong Chen,Liezhao Cao,Jean-Claude Lasjaunias
Physics , 2010, DOI: 10.1143/JPSJ.78.063602
Abstract: We report thermal transport measurements performed on the quasi-one-dimensional Bechgaard salts (TMTSF)2ClO4 and (TMTSF)2PF6 along the a-direction. For both salts, magnon-drag effects are found to contribute considerably to thermopower above 80 K. These results imply spin-charge separation in the metallic state for both salts. Moreover, a linear temperature-dependent thermal conductivity is found to be unaffected by anion disorder below an anion ordering transition temperature TAO~25 K in (TMTSF)2ClO4.
A Companion Cell–Dominant and Developmentally Regulated H3K4 Demethylase Controls Flowering Time in Arabidopsis via the Repression of FLC Expression
Hongchun Yang,Zhifu Han,Ying Cao,Di Fan,Hong Li,Huixian Mo,Yi Feng,Lei Liu,Zheng Wang,Yanling Yue,Sujuan Cui,She Chen,Jijie Chai,Ligeng Ma
PLOS Genetics , 2012, DOI: 10.1371/journal.pgen.1002664
Abstract: Flowering time relies on the integration of intrinsic developmental cues and environmental signals. FLC and its downstream target FT are key players in the floral transition in Arabidopsis. Here, we characterized the expression pattern and function of JMJ18, a novel JmjC domain-containing histone H3K4 demethylase gene in Arabidopsis. JMJ18 was dominantly expressed in companion cells; its temporal expression pattern was negatively and positively correlated with that of FLC and FT, respectively, during vegetative development. Mutations in JMJ18 resulted in a weak late-flowering phenotype, while JMJ18 overexpressors exhibited an obvious early-flowering phenotype. JMJ18 displayed demethylase activity toward H3K4me3 and H3K4me2, and bound FLC chromatin directly. The levels of H3K4me3 and H3K4me2 in chromatins of FLC clade genes and the expression of FLC clade genes were reduced, whereas FT expression was induced and the protein expression of FT increased in JMJ18 overexpressor lines. The early-flowering phenotype caused by the overexpression of JMJ18 was mainly dependent on the functional FT. Our findings suggest that the companion cell–dominant and developmentally regulated JMJ18 binds directly to the FLC locus, reducing the level of H3K4 methylation in FLC chromatin and repressing the expression of FLC, thereby promoting the expression of FT in companion cells to stimulate flowering.
Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells
Shan Tian,Yulong Bai,Lin Yang,Xinggang Wang,Yi Wu,Jie Jia,Yulian Zhu,Yong Cheng,Pengyue Zhang,Junfa Wu,Nianhong Wang,Guang Xia,Hua Liao,Yuling Zhang,Xiafeng Shen,Huixian Yu,Yongshan Hu
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms14011412
Abstract: As a therapeutic strategy for ischemic stroke, to restore or increase cerebral blood flow (CBF) is the most fundamental option. Laminar shear stress (LS), as an important force generated by CBF, mainly acts on brain microvascular endothelial cells (BMECs). In order to study whether LS was a protective factor in stroke, we investigated LS-intervented ischemic apoptosis of rat BMECs (rBMECs) through PE Annexin V/7-AAD, JC-1 and Hoechst 33258 staining to observe the membranous, mitochondrial and nuclear dysfunction. Real-time PCR and western blot were also used to test the gene and protein expressions of Tie-2, Bcl-2 and Akt, which were respectively related to maintain membranous, mitochondrial and nuclear norm. The results showed that LS could be a helpful stimulus for ischemic rBMECs survival. Simultaneously, membranous, mitochondrial and nuclear regulation played an important role in this process.
Virus-induced gene-silencing in wheat spikes and grains and its application in functional analysis of HMW-GS-encoding genes
Meng Ma, Yan Yan, Li Huang, Mingshun Chen, Huixian Zhao
BMC Plant Biology , 2012, DOI: 10.1186/1471-2229-12-141
Abstract: Apparent photobleaching on the spikes infected with BSMV:PDS at heading stage was observed after13 days post inoculation (dpi), and persisted until 30dpi, while the spikes inoculated with BSMV:00 remained green during the same period. Grains of BSMV:PDS infected spikes also exhibited photobleaching. Molecular analysis indicated that photobleached spikes or grains resulted from the reduction of endogenous PDS transcript abundances, suggesting that BSMV:PDS was able to induce PDS silencing in wheat spikes and grains. Inoculation onto wheat spikes from heading to flowering stage was optimal for efficient silencing of PDS in wheat spikes. Furthermore, we used the BSMV-based system to reduce the transcript level of 1Bx14, a gene encoding for High-molecular-weight glutenin subunit 1Bx14 (HMW-GS 1Bx14), by 97?% in the grains of the BSMV:1Bx14 infected spikes at 15dpi, compared with that in BSMV:00 infected spikes, and the reduction persisted until at least 25 dpi. The amount of the HMW-GS 1Bx14 was also detectably decreased. The percentage of glutenin macropolymeric proteins in total proteins was significantly reduced in the grains of 1Bx14-silenced plants as compared with that in the grains of BSMV:00 infected control plants, indicating that HMW-GS 1Bx14 is one of major components participating in the formation of glutenin macropolymers in wheat grains.This is one of the first reports of successful application of BSMV-based virus-induced-gene-silencing (VIGS) for gene knockdown in wheat spikes and grains and its application in functional analysis of the 1Bx14 gene. The established BSMV-VIGS system will be very useful in future research on functional analysis of genes contributing to grain quality and the metabolic networks in developing seeds of wheat.Wheat (Triticum aestivum L.) is one of the major staple crops for the human diet. With the increase in the global population, the shortage of foods has become more and more serious. Therefore, wheat yield and quality improve
Characterization of a Polyamine Microsphere and Its Adsorption for Protein
Feng Wang,Pei Liu,Tingting Nie,Huixian Wei,Zhenggang Cui
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms14010017
Abstract: A novel polyamine microsphere, prepared from the water-in-oil emulsion of polyethylenimine, was characterized. The investigation of scanning electron microscopy showed that the polyamine microsphere is a regular ball with a smooth surface. The diameter distribution of the microsphere is 0.37–4.29 μm. The isoelectric point of the microsphere is 10.6. The microsphere can adsorb proteins through the co-effect of electrostatic and hydrophobic interactions. Among the proteins tested, the highest value of adsorption of microsphere, 127.8 mg·g ?1 microsphere, was obtained with lipase. In comparison with other proteins, the hydrophobic force is more important in promoting the adsorption of lipase. The microsphere can preferentially adsorb lipase from an even mixture of proteins. The optimum temperature and pH for the selective adsorption of lipase by the microsphere was 35 °C and pH 7.0.
IL-11 produced by breast cancer cells augments osteoclastogenesis by sustaining the pool of osteoclast progenitor cells
McCoy Erin M,Hong Huixian,Pruitt Hawley C,Feng Xu
BMC Cancer , 2013, DOI: 10.1186/1471-2407-13-16
Abstract: Background Interleukin (IL)-11, a cytokine produced by breast cancer, has been implicated in breast cancer-induced osteolysis (bone destruction) but the mechanism(s) of action remain controversial. Some studies show that IL-11 is able to promote osteoclast formation independent of the receptor activator of NF-κB ligand (RANKL), while others demonstrate IL-11 can induce osteoclast formation by inducing osteoblasts to secrete RANKL. This work aims to further investigate the role of IL-11 in metastasis-induced osteolysis by addressing a new hypothesis that IL-11 exerts effects on osteoclast progenitor cells. Methods To address the precise role of breast cancer-derived IL-11 in osteoclastogenesis, we determined the effect of breast cancer conditioned media on osteoclast progenitor cells with or without an IL-11 neutralizing antibody. We next investigated whether recombinant IL-11 exerts effects on osteoclast progenitor cells and survival of mature osteoclasts. Finally, we examined the ability of IL-11 to mediate osteoclast formation in tissue culture dishes and on bone slices in the absence of RANKL, with suboptimal levels of RANKL, or from RANKL-pretreated murine bone marrow macrophages (BMMs). Results We found that freshly isolated murine bone marrow cells cultured in the presence of breast cancer conditioned media for 6 days gave rise to a population of cells which were able to form osteoclasts upon treatment with RANKL and M-CSF. Moreover, a neutralizing anti-IL-11 antibody significantly inhibited the ability of breast cancer conditioned media to promote the development and/or survival of osteoclast progenitor cells. Similarly, recombinant IL-11 was able to sustain a population of osteoclast progenitor cells. However, IL-11 was unable to exert any effect on osteoclast survival, induce osteoclastogenesis independent of RANKL, or promote osteoclastogenesis in suboptimal RANKL conditions. Conclusions Our data indicate that a) IL-11 plays an important role in osteoclastogenesis by stimulating the development and/or survival of osteoclast progenitor cells and b) breast cancer may promote osteolysis in part by increasing the pool of osteoclast progenitor cells via tumor cell-derived IL-11. However, given the heterogeneous nature of the bone marrow cells, the precise mechanism by which IL-11 treatment gives rise to a population of osteoclast progenitor cells warrants further investigation.
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