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Search Results: 1 - 10 of 5997 matches for " Huangxian Ju "
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Grand challenges in analytical chemistry: towards more bright eyes for scientific research, social events and human health
Huangxian Ju
Frontiers in Chemistry , 2013, DOI: 10.3389/fchem.2013.00005
Abstract:
DNA Electrochemical Behaviors, Recognition and Sensing by Combining with PCR Technique
Yongkang Ye,Huangxian Ju
Sensors , 2003, DOI: 10.3390/s30600128
Abstract: The electrochemical studies on the behaviors and recognition of DNA have attracted cosiderable attention. DNA biosensors based on a nucleic acid hybridization process are rapidly being developed towards the goal of rapid and inexpensive diagnosis of genetic andinfectious deseases. This brief review focuses on the current state of the DNA electrochemical sensors with emphasis on recent advances, challanges and trends. The works on DNA electrochemical behaviors, recognition and detection in our group in the last three years are also introduced.
Preface for Publication of the Special Issue of I3S 2004
Hongyuan Chen,Huangxian Ju
Sensors , 2005, DOI: 10.3390/s5040160
Abstract: No abstract available
A Novel Hydrogen Peroxide Sensor via the Direct Electrochemistry of Horseradish Peroxidase Immobilized on Colloidal Gold Modified Screen-printed Electrode
Xiaoxing Xu,Songqin Liu,Huangxian Ju
Sensors , 2003, DOI: 10.3390/s30900350
Abstract: The direct electrochemistry of horseradish peroxidase (HRP) immobilized on a colloidal gold modified screen-printed carbon electrode (HRP-Au-SPCE) and its application as a disposable sensor were studied. The immobilized HRP displayed a couple of stable and well-defined redox peaks with a formal potential of –0.338 V (vs. SCE) and a heterogeneous electron transfer rate constant of (0.75±0.04) s-1 in 0.1 M pH 7.0 PBS. It showed a highly thermal stability, fast amperometric response and an electrocatalytic activity to the reduction of hydrogen peroxide (H2O2) without the aid of an electron mediator. The biosensor exhibited high sensitivity, good reproducibility, and long-term stability for the determination of H2O2 with a linear range from 0.8 μM to 1.0 mM and a detection limit of 0. 4 μM at 3σ. The variation coefficients are 2.7 % and 2.3 % for over 10 successive assays at the H2O2 concentrations of 8.0 and 20 μM, respectively. The K M app for H2O2 sensor was determined to be 1.3 mM.
A Novel Supramolecular Assembly Film of Porphyrin Bound DNA: Characterization and Catalytic Behaviors Towards Nitric Oxide
Jianping Lei,Huangxian Ju,Osamu Ikeda
Sensors , 2005, DOI: 10.3390/s5040171
Abstract: A stable Fe(4-TMPyP)-DNA-PADDA (FePyDP) film was characterized onpyrolytic graphite electrode (PGE) or an indium-tin oxide (ITO) electrode through thesupramolecular interaction between water-soluble iron porphyrin (Fe(4-TMPyP)) and DNAtemplate, where PADDA (poly(acrylamide-co-diallyldimethylammonium chloride) isemployed as a co-immobilizing polymer. Cyclic voltammetry of FePyDP film showed a pairof reversible FeIII/FeII redox peaks and an irreversible FeIV/FeIII peak at –0.13 V and 0.89vs. Ag|AgCl in pH 7.4 PBS, respectively. An excellent catalytic reduction of NO wasdisplayed at –0.61 V vs. Ag|AgCl at a FePyDP film modified electrode.Chronoamperometric experiments demonstrated a rapid response to the reduction of NOwith a linear range from 0.1 to 90 μM and a detection limit of 30 nM at a signal-to-noiseratio of 3. On the other hand, it is the first time to apply high-valent iron porphyrin ascatalyst at modified electrode for NO catalytic oxidation at 0.89 vs. Ag|AgCl. The sensorshows a high selectivity of some endogenous electroactive substances in biological systems.The mechanism of response of the sensors to NO is preliminary studied.
Supermolecular Interaction of Ferrocenium with Yeast DNA and Application in Electrochemical Sensing for Hybridization Recognition of Yeast DNA
Huangxian Ju,Baofen Ye,Jiayin Gu
Sensors , 2004, DOI: 10.3390/s40500071
Abstract: The supermolecular interactions of single-stranded yeast DNA (ssDNA) and double-stranded DNA (dsDNA) with ferrocenium (Fc+) both in solution and at the electrode/solution interface were studied with UV spectroscopy and electrochemical method. The interaction of covalently immobilized dsDNA with Fc+ resulted in a change of the electrode behavior from diffusion-controlled to surface-controlled and an increase in the current response of Fc+ reduction, which has been used to develop a novel electrochemical yeast DNA sensor for hybridization recognition of immobilized yeast ssDNA to its complementary ssDNA (cDNA). The adsorption constants of Fc+ on ssDNA and dsDNA modified gold electrode surface were (3.38±0.04)×103 M-1 and (2.02±0.02)×104 M-1, respectively, indicating a higher affinity of dsDNA to Fc+. UV spectra and the influence of ion strength showed that the interaction mode between Fc+ and dsDNA in solution might be a groove binding. The interaction between Fc+ and immobilized DNA was mainly an electrostatic model.
Extraction and Determination of Short-Chain Fatty Acids in Biological Samples
生物样品中短链脂肪酸的提取与测定

TAN Li,JU Huangxian,LI Jieshou,
谭力
,鞠熀先,黎介寿

色谱 , 2006,
Abstract: Short-chain fatty acids are organic acids with 1-6 carbon atoms.Their physiological functions and clinical applications have attracted considerable attention. Their high polarity,low ultraviolet(UV) absorbance,low contents in biological samples,high volatility,good(water)-solubility and easy adsorption on metal and glass surfaces result in some difficulties in their separation and determination.This review focuses on the methods for extraction of short-chain fatty acids in biological samples such as feces,urine,blood and culture solutions by(distillation),high speed centrifugation,liquid-solid extraction,solid phase microextration,(supercritical) fluid extraction and liquid phase microextraction and determination by gas chromatography,high performance liquid chromatography and capillary electrophoresis.Sixty-three references are cited.
Functionalized Graphene Oxide Mediated Adriamycin Delivery and miR-21 Gene Silencing to Overcome Tumor Multidrug Resistance In Vitro
Feng Zhi, Haifeng Dong, Xuefeng Jia, Wenjie Guo, Huiting Lu, Yilin Yang, Huangxian Ju, Xueji Zhang, Yiqiao Hu
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0060034
Abstract: Multidrug resistance (MDR) is a major impediment to successful cancer chemotherapy. Co-delivery of novel MDR-reversing agents and anticancer drugs to cancer cells holds great promise for cancer treatment. MicroRNA-21 (miR-21) overexpression is associated with the development and progression of MDR in breast cancer, and it is emerging as a novel and promising MDR-reversing target. In this study, a multifunctional nanocomplex, composed of polyethylenimine (PEI)/poly(sodium 4-styrenesulfonates) (PSS)/graphene oxide (GO) and termed PPG, was prepared using the layer-by-layer assembly method to evaluate the reversal effects of PPG as a carrier for adriamycin (ADR) along with miR-21 targeted siRNA (anti-miR-21) in cancer drug resistance. ADR was firstly loaded onto the PPG surface (PPGADR) by physical mixing and anti-miR-21 was sequentially loaded onto PPGADR through electric absorption to form anti-miR-21PPGADR. Cell experiments showed that PPG significantly enhanced the accumulation of ADR in MCF-7/ADR cells (an ADR resistant breast cancer cell line) and exhibited much higher cytotoxicity than free ADR, suggesting that PPG could effectively reverse ADR resistance of MCF-7/ADR. Furthermore, the enhanced therapeutic efficacy of PPG could be correlated with effective silencing of miR-21 and with increased accumulation of ADR in drug-resistant tumor cells. The endocytosis study confirmed that PPG could effectively carry drug molecules into cells via the caveolae and clathrin-mediated endocytosis pathways. These results suggest that this PPG could be a potential and efficient non-viral vector for reversing MDR, and the strategy of combining anticancer drugs with miRNA therapy to overcome MDR could be an attractive approach in cancer treatment.
The Enlightenment of Marxist Saving Thoughts on the Revitalization of Rural Ecology in China  [PDF]
Yang Ju
Chinese Studies (ChnStd) , 2018, DOI: 10.4236/chnstd.2018.72013
Abstract: On October 18, 2017, Comrade Jinping Xi proposed a strategy for the revitalization of villages in the report of the 19th CPC National Congress. The issue of agriculture and rural peasants is a fundamental issue related to the national economy and the people’s livelihood. The issue of solving the “three rural issues” must always be regarded as a top priority for the work of the whole Party. Therefore, the strategy of rejuvenating the country is proposed. The problems to be solved in the process of revitalization of rural ecology in China are closely related to Marx’s idea of economy in “Das Kapital”. From the perspective of Marx’s saving ideas, this article combines the content of China’s rural revitalization strategy and sums up Marx’s idea of conservation, drawing an important revelation for in the Process of Revitalizing Rural Ecosystem in China.
Study on Bifurcation and Chaos in Boost Converter Based on Energy Balance Model  [PDF]
Quanmin NIU, Zhizhong JU
Energy and Power Engineering (EPE) , 2009, DOI: 10.4236/epe.2009.11006
Abstract: Based on boost converter operating in discontinuous mode, this paper proposes an energy balance model (EBM) for analyzing bifurcation and chaos phenomena of capacitor energy and output voltage when the converter parameter is varying. It is found that the capacitor energy and output voltage dynamic behaviors exhibit the typical period-doubling route to chaos by increasing the feedback gain constant K of proportional controller. The accurate position of the first bifurcation point and the iterative diagram of the capacitor energy with every K can be derived from EBM. Finally, the underlying causes for bifurcations and chaos of a general class of nonlinear systems such as power converters are analyzed from the energy balance viewpoint. Com-paring with the discrete iterative model, EBM is simple and high accuracy. This model can be easily devel-oped on the nonlinear study of the other converters.
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