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sauce by a high salt liquid-state fermentation process was prepared using the organic soy beans as raw material. Beidahuang
organic soy bean was selected among different raw materials. Here, the best
technique was determined. Firstly, the organic soy beans were soaked for 7
hours under 121℃, steamed for 15 min and mixed with
the fried wheat (5:5, w/w). After inoculated with Aspergillus oryzae 3.042 (0.3%, w/w) and cultured for 36 hours, the
koji was obtained. When the brine (2:1, w/w) was added, fermentation started. At
the end of the fermentation, isoflavone content of organic soy sauce was 0.22 mg· g-1 higher than those in the non- organic soy beans. In addition, compared to the
control there were a higher unsaturated fatty acids content, the linoleic acid
content in crude fat of 51.61% and
γ-linolenic acid content in crude fat of 0.55%.
There exist a large number of
composed documents in universities in the teaching process. Most of them are
required to check the similarity for validation. A kind of similarity
computation system is constructed for composed documents with images and text
information. Firstly, each document is split and outputs two parts as images
and text information. Then, these documents are compared by computing the
similarities of images and text contents independently. Through Hadoop system,
the text contents are easily and quickly separated. Experimental results show
that the proposed system is efficient and practical.
In this study, we, for the first time, tried to apply IC50 values (inhibitory concentration estimated to affect the endpoint in question by 50%) in the MTT colorimetric assay to investigate the cytotoxic effects of highly absorbent foam dressings based on silver zirconium phosphate, a newly nano-based matrix. Our results showed that silver released from dressings based on silver zirconium phosphate attributed mainly to highly cytotoxic to L929 cells cultured with MEM containing 10% fetal bovine serum. In addition, we have also compared the IC50 values among different dilutions of AgNO3 solution, silver based dressing extracts and material reference control (ZDEC) extracts using the optimized MTT assay, along with characterizing the silver content in the dressing extracts using atomic absorption spectroscopy. Results have shown that the IC50 values of AgNO50 solution, silver based dressing extracts and ZDEC extracts are 3.5 μg/mL, 3.8 μg/mL and 8.4%, respectively. And there exist some good agreements between qualitative and quantitative evaluation method as well. In conclusion, our study has led to the view that the IC50 value is a promising quantitative index for screening cytotoxicity with regard to silver based dressings.
This study was conducted to determine the effect of
different starter diets and different amounts of milk on growth performance and
gastrointestinal tract development in unweaned calves. 16 calves were assigned
to 4 groups, 4 calves in each group. These four groups received the following
treatments respectively: 1) high milk (6 L) + low starch (21%), high NDF (28%),
high molasses (10%) starter (HMLS group); 2) high milk (6L) + high starch
(40%), low NDF (14%), low molasses (5%) starter (HMHS group); 3) low milk (3 L)
+ low starch (21%), high NDF (28%), high molasses (10%) starter (LMLS group); 4)
low milk (3 L) + high starch (40%), low NDF (14%), low molasses (5%) starter
(LMHS group). The trial was of 2 × 2 factorial design. All calves had free
access to hay and water. Results showed that the low milk allowance increased
calf concentrate dry matter intake (DMI) and total DMI, reduced body height at
4 weeks of age, reduced heart girth at 6 weeks of age (P < 0.05). The low milk allowance increased complex stomach full
weight, reticulorumen full weight, and the percentages of stomach full weight
and reticulorumen full weight over body weight (P < 0.05). The low starch, high fiber, high molasses starters
reduced the complex stomach full weight, the proportion of the complex stomach
empty weight over body weight, reticulorumen empty weight, abomasum full
weight, the proportion of abomasum empty weight over body weight and
reticulorumen volume (P < 0.05).
The low milk allowance increased calf intestine length, small intestine full
weight, the proportion of small intestine full weight over body weight, but
reduced the jejunum villus width (P <
0.05), increased ruminal pH (P < 0.05).
The low milk allowance reduced papilla length in rumen anterior ventral blind
sac, but increased papilla length in the posterior ventral sac (P < 0.05).