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Search Results: 1 - 10 of 49960 matches for " Hou Wu "
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A trinuclear cobalt–cerium complex: bis(2,2′-bipyridine)-1κ2N,N′;3κ2N,N′-hexa-μ-methacrylato-1:2κ6O:O′;2:3κ6O:O′-nitrato-2κ2O,O′-2-cerium(III)-1,3-dicobalt(II)
Bin Wu,Tingting Hou
Acta Crystallographica Section E , 2010, DOI: 10.1107/s1600536810010299
Abstract: In the title trinuclear cobalt-cerium complex, [CeCo2(C4H5O2)6(NO3)(C10H8N2)2], the CeIII and each of the two CoII ions are bridged by three bidentate methacrylate ligands. The CeIII center is coordinated by six O atoms from six methacrylate ligands and two O atoms from the nitrate anion in a distorted square-antiprismatic geometry. Each CoII ion is coordinated by three O atoms from three methacrylate ligands and two N atoms from a 2,2′-bipyridine ligand in a distorted trigonal-pyramidal geometry. In the crystal structure, π–π interactions between the aromatic rings [centroid–centroid distances of 3.816 (8) and 3.756 (8) ] link the molecules into chains propagated in [01overline{1}]. Weak intermolecular C—H...O hydrogen bonds further stabilize the crystal packing.
The development of soy sauce from organic soy bean  [PDF]
Shoupeng Wan, Yanxiang Wu, Cong Wang, Chunling Wang, Lihua Hou
Agricultural Sciences (AS) , 2013, DOI: 10.4236/as.2013.45B022
Abstract:

Soy sauce by a high salt liquid-state fermentation process was prepared using the organic soy beans as raw material. Beidahuang organic soy bean was selected among different raw materials. Here, the best technique was determined. Firstly, the organic soy beans were soaked for 7 hours under 121℃, steamed for 15 min and mixed with the fried wheat (5:5, w/w). After inoculated with Aspergillus oryzae 3.042 (0.3%, w/w) and cultured for 36 hours, the koji was obtained. When the brine (2:1, w/w) was added, fermentation started. At the end of the fermentation, isoflavone content of organic soy sauce was 0.22 mg· g-1 higher than those in the non- organic soy beans. In addition, compared to the control there were a higher unsaturated fatty acids content, the linoleic acid content in crude fat of 51.61% and γ-linolenic acid content in crude fat of 0.55%.

Generation of a recombinant herpes simplex virus which can provide packaging function for recombinant adeno-associated virus
Zhijian Wu,Xiaobing Wu,Yunde Hou
Chinese Science Bulletin , 1999, DOI: 10.1007/BF02909710
Abstract: The generation of a recombinant HSV (rHSV) that can provide packaging function for rAAV production is described. A set of cosmids including cos48, cos28, cos6, cosl4 and cos56, which represents the HSV-1 genome was used for generation of this rHSV.Rep andcap genes of AAV-2 were inserted intoXba I site ofUL2 gene on cod, generating cos6rcΔUL2. After being digested withPac I, cos6-rcΔUL2 and the other 4 cosmids were cotransfected into BHK-21 cells. The recombinant virus HSV1-rc/ΔUL2 carryingrep andcap genes was generated due to the homologous recombination of the 5 cosmids. The results showed that the existence ofrep andcap genes on this rHSV was stable from passage to passage and the rHSV could support the packaging of rAAV either in cells transiently transfected with AAV vector or in stable cell line harboring AAV vector. Further modification of this rHSV and optimization of conditions involved in rAAV preparation may lead to a large-scale production of rAAV in the near future.
Hadoop-Based Similarity Computation System for Composed Documents  [PDF]
Xiaoming Zhang, Zhipeng Qin, Xuwei Liu, Qianyun Hou, Baishuang Zhang, Jie Wu
Journal of Computer and Communications (JCC) , 2015, DOI: 10.4236/jcc.2015.35025
Abstract:

There exist a large number of composed documents in universities in the teaching process. Most of them are required to check the similarity for validation. A kind of similarity computation system is constructed for composed documents with images and text information. Firstly, each document is split and outputs two parts as images and text information. Then, these documents are compared by computing the similarities of images and text contents independently. Through Hadoop system, the text contents are easily and quickly separated. Experimental results show that the proposed system is efficient and practical.

The Effects of Amino Acid Nutritional Deficiency on the Expression of Protein Metabolism-Related Genes in the Mammary Gland and Muscle Tissues of Lactating Mice  [PDF]
Xueyan Lin, Miaomiao Wu, Guimei Liu, Yun Wang, Qiuling Hou, Kerong Shi, Zhonghua Wang
Advances in Bioscience and Biotechnology (ABB) , 2015, DOI: 10.4236/abb.2015.69063
Abstract: The mammary gland tissue exhibits a series of responses that are different from those of muscle and other peripheral tissues under amino acid deficiency. So, this present study was designed to investigate the effects of amino acid nutritional deficiency on the expression of protein metabolism-related genes in the mammary gland and muscle tissues of lactating mice. A total of 60 postpartum, lactating Kunming white mice were selected and randomly divided into 5 groups; each group contained 12 mice. Group A was the control group. The mice in group A were maintained on a normal diet after the initiation of lactation. Group B (starved) was given normal saline via intragastric administration. Group C (energy) was given glucose solution via intragastric administration. Groups D and E received a sodium caseinate solution via intragastric administration, which provided 0.5 g protein/d and 1.5 g protein/d, respectively. The results showed the following. 1) When the mice were exposed to nutritional stress caused by dietary amino acid deficiency, the β-casein mRNA expression level was increased in the mammary gland tissue. The increase in β-casein expression was the most significant in the energy-supplemented group, followed by the starved group (P < 0.01). In addition, the expression level of myosin heavy chain 2A (MHC2a) mRNA was markedly reduced in the muscle tissue (P < 0.05). 2) Different dietary treatments significantly affected the mRNA expression of ubiquitin system enzymes such as PolyUbC, E214k and C2 (P < 0.01). The expression levels of the enzymes were upregulated. 3) The blood level of insulin-like growth factor 1 (IGF-1) was significantly decreased in the treatment groups (P < 0.01). 4) The phosphorylation level of the p70S6 kinase (p70S6K) was markedly enhanced in the mammary gland tissues collected from the treatment groups (P < 0.05). 5) The phosphorylation level of p70S6K was elevated in the muscle tissues collected from the treatment groups. However, the magnitude of the increase was far smaller compared to that in the mammary gland tissues.
In Vitro Cytotoxicity Evaluation of Highly Absorbent Foam Dressings Based on Silver Zirconium Phosphate via IC50 Value  [PDF]
Chenghu Liu, Li Hou, Lili Liu, Zhonghua Qu, Xin Wang, Xiaoxia Sun, Ping Wu, Yanping Shi
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2016, DOI: 10.4236/jbnb.2016.71005
Abstract:

In this study, we, for the first time, tried to apply IC50 values (inhibitory concentration estimated to affect the endpoint in question by 50%) in the MTT colorimetric assay to investigate the cytotoxic effects of highly absorbent foam dressings based on silver zirconium phosphate, a newly nano-based matrix. Our results showed that silver released from dressings based on silver zirconium phosphate attributed mainly to highly cytotoxic to L929 cells cultured with MEM containing 10% fetal bovine serum. In addition, we have also compared the IC50 values among different dilutions of AgNO3 solution, silver based dressing extracts and material reference control (ZDEC) extracts using the optimized MTT assay, along with characterizing the silver content in the dressing extracts using atomic absorption spectroscopy. Results have shown that the IC50 values of AgNO50 solution, silver based dressing extracts and ZDEC extracts are 3.5 μg/mL, 3.8 μg/mL and 8.4%, respectively. And there exist some good agreements between qualitative and quantitative evaluation method as well. In conclusion, our study has led to the view that the IC50 value is a promising quantitative index for screening cytotoxicity with regard to silver based dressings.

The Analytical Expressions for Computing the Minimum Distance between a Point and a Torus  [PDF]
Xiaowu Li, Linke Hou, Juan Liang, Zhinan Wu, Lin Wang, Chunguang Yue
Journal of Computer and Communications (JCC) , 2016, DOI: 10.4236/jcc.2016.44011
Abstract: In this paper, we present the analytical expressions for computing the minimum distance between a point and a torus, which is called the orthogonal projection point problem. If the test point is on the outside of the torus and the test point is at the center axis of the torus, we present that the orthogonal projection point set is a circle perpendicular to the center axis of the torus; if not, the analytical expression for the orthogonal projection point problem is also given. Furthermore, if the test point is in the inside of the torus, we also give the corresponding analytical expression for orthogonal projection point for two cases.
Gastrointestinal Tract Development in Unweaned Calves Feeding Different Amounts of Milk and Different Starters  [PDF]
Tian Zhang, Zhiqiang Wu, Qiuling Hou, Yun Wang, Zhiyong Hu, Xueyan Lin, Zhonghua Wang
Advances in Bioscience and Biotechnology (ABB) , 2018, DOI: 10.4236/abb.2018.97019
Abstract:

This study was conducted to determine the effect of different starter diets and different amounts of milk on growth performance and gastrointestinal tract development in unweaned calves. 16 calves were assigned to 4 groups, 4 calves in each group. These four groups received the following treatments respectively: 1) high milk (6 L) + low starch (21%), high NDF (28%), high molasses (10%) starter (HMLS group); 2) high milk (6L) + high starch (40%), low NDF (14%), low molasses (5%) starter (HMHS group); 3) low milk (3 L) + low starch (21%), high NDF (28%), high molasses (10%) starter (LMLS group); 4) low milk (3 L) + high starch (40%), low NDF (14%), low molasses (5%) starter (LMHS group). The trial was of 2 × 2 factorial design. All calves had free access to hay and water. Results showed that the low milk allowance increased calf concentrate dry matter intake (DMI) and total DMI, reduced body height at 4 weeks of age, reduced heart girth at 6 weeks of age (P < 0.05). The low milk allowance increased complex stomach full weight, reticulorumen full weight, and the percentages of stomach full weight and reticulorumen full weight over body weight (P < 0.05). The low starch, high fiber, high molasses starters reduced the complex stomach full weight, the proportion of the complex stomach empty weight over body weight, reticulorumen empty weight, abomasum full weight, the proportion of abomasum empty weight over body weight and reticulorumen volume (P < 0.05). The low milk allowance increased calf intestine length, small intestine full weight, the proportion of small intestine full weight over body weight, but reduced the jejunum villus width (P < 0.05), increased ruminal pH (P < 0.05). The low milk allowance reduced papilla length in rumen anterior ventral blind sac, but increased papilla length in the posterior ventral sac (P < 0.05).

A Haplotype Block Model for Fine Mapping of Quantitative Trait Loci Regulating HIV-1 Pathogenesis
Yun Zhu,Wei Hou,Rongling Wu
Computational and Mathematical Methods in Medicine , 2003, DOI: 10.1080/10273660412331319486
Abstract: The dynamic change of human immunodeficiency virus type-1 (HIV-1) particles that cause AIDS displays considerable variation from patients to patients. It is likely that such variation in HIV-1 pathogenesis is correlated with the genetic architecture of hosts. Traditional genetic analysis of HIV-1 infection is based on various biochemical approaches, but it has been little successful because HIV-1 dynamics, as a complex trait, is under polygenic control and sensitive to environmental changes. Here, we present a novel model for integrating mathematical functions for HIV-1 dynamics that have been well constructed into a multivariate mixture model for genetic mapping. This integrative mapping model on the foundation of linkage disequilibrium (LD)-based haplotype block analysis provides unique power to precisely detect human quantitative trait loci (QTL) determining HIV-1 dynamics and facilitates positional cloning of target QTL. The model allows for a number of hypothesis tests for the effects of the dynamic QTL on the virion clearance rate, the infected cell life-span and the average viral generation time in vivo, all of which provide theoretical principles to guide the development of efficient gene therapy strategies.
2-Methoxy-N-[(S)-3-methylbutan-2-yl]-6-{[(S)-3-methylbutan-2-yl]amino}-3,5-dinitrobenzamide
Xuefen Wu,Xi Chen,Yimin Hou
Acta Crystallographica Section E , 2011, DOI: 10.1107/s1600536811049749
Abstract: The title compound, C18H28N4O6, crystallizes with two molecules in the asymmetric unit which differ slightly in conformation. The dihedral angle between the amide plane and the benzene ring are 72.6 (2) and 66.8 (2)° in the two molecules. A strong intramolecular N—H...O hydrogen bond between the amino and nitro groups occurs in each molecule. The crystal structure features two symmetry-independent polymeric chains along [010] generated by N—H...O hydrogen bonds between the amide groups.
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